>AFA156 (AFA156Q) /CSM/AF/AFA1-C/AFA156Q.Seq.d/
TGTTGGCCTACTGGNATTTTTATTTTTTTTCAAATATTTGTTTAACTCATATAAAGTGAG
AAAGACAAGTGTACATAAAAACTTGATCTTTTTTATTTTATATCACACACATACATAATA
AAAAACTATGGCAACTCAACAACAACAACAACAACAACAACAACAACAACAGCAAATTAA
GGCTAGAAAAGATATACAAATTCAACAAGCACAATCTGCTTCTGATATATTAGGACCACC
AGAAATTTCAGAAACTGAAATTACAACAGAATCAATACTTGGTGATGGAAGTTTTGGTAC
TGTTTATAAAGGTCGTTGTAGATTAAAGGATGTAGCAGTAAAGGTTATGTTAAAACAAGT
CGATCAAAAGACTTTAACTGATTTCAGAAAGGAAGTTGCAATTATGAGTAAAATTTTTCA
TCCAAATATTGTACTTTTTTTAGGTGCATGTACATCTACACCAGGTAAACTTATGATTTG
TACAGAATTAATGAAAGGTAATCTTGAATCATTATTATTAGACCCAATGGTTAAACTCCC
ATTGATCACTCGTATGAGAATGGCAAAAGATGCAGCTTTAGGTGTTTTATGGTTACATTC
TTCAXXXXXXXXXX

vgllxflfffkylfnsykvrktsvhknliffilyhtht**KTMATQQQQQQQQQQQQQIK
ARKDIQIQQAQSASDILGPPEISETEITTESILGDGSFGTVYKGRCRLKDVAVKVMLKQV
DQKTLTDFRKEVAIMSKIFHPNIVLFLGACTSTPGKLMICTELMKGNLESLLLDPMVKLP
LITRMRMAKDAALGVLWLHS---

Frame A:
cwptgififfqifv*li*sekdkct*kldlfyfishtyiiknygnstttttttttttan*
g*krytnststicf*yirttrnfrn*nynrintw*wkfwycl*rsl*ikgcsskgyvkts
rskdfn*fqkgscnye*nfsskyctffrcmyiytr*tydlyriner*s*iiiirpng*tp
idhsyengkrcsfrcfmvtff---


Frame B:
vgllxflfffkylfnsykvrktsvhknliffilyhtht**KTMATQQQQQQQQQQQQQIK
ARKDIQIQQAQSASDILGPPEISETEITTESILGDGSFGTVYKGRCRLKDVAVKVMLKQV
DQKTLTDFRKEVAIMSKIFHPNIVLFLGACTSTPGKLMICTELMKGNLESLLLDPMVKLP
LITRMRMAKDAALGVLWLHS---


Frame C:
laywxfyffsniclthik*erqvyikt*sflfyithihnkklwqlnnnnnnnnnnnsklr
lekiykfnkhnllliy*dhqkfqklklqqnqylvmevlvlfikvvvd*rm*q*rlc*nks
ikrl*liserklql*vkffiqilyff*vhvhlhqvnl*fvqn**kvilnhyy*tqwlnsh
*slv*ewqkmql*vfygyil---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO835 (VFO835Q) /CSM/VF/VFO8-B/VFO835Q.Seq.d/                        817   0.0
AFM648 (AFM648Q) /CSM/AF/AFM6-B/AFM648Q.Seq.d/                        817   0.0
AFM201 (AFM201Q) /CSM/AF/AFM2-A/AFM201Q.Seq.d/                        817   0.0
AFK731 (AFK731Q) /CSM/AF/AFK7-B/AFK731Q.Seq.d/                        817   0.0
AFF486 (AFF486Q) /CSM/AF/AFF4-D/AFF486Q.Seq.d/                        817   0.0
AFE822 (AFE822Q) /CSM/AF/AFE8-A/AFE822Q.Seq.d/                        817   0.0
AFA156 (AFA156Q) /CSM/AF/AFA1-C/AFA156Q.Seq.d/                        817   0.0
VFK734 (VFK734Q) /CSM/VF/VFK7-B/VFK734Q.Seq.d/                        809   0.0
VFD621 (VFD621Q) /CSM/VF/VFD6-A/VFD621Q.Seq.d/                        809   0.0
CHR241 (CHR241Q) /CSM/CH/CHR2-B/CHR241Q.Seq.d/                        809   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ297966|AJ297966.1 Dictyostelium discoideum mRNA for SHK1 protein.   490  0.0  2
BU582405|BU582405.1 PL N82 P.lunula ZAP-cDNA library Pyrocystis lunula cDNA clone 731 5' similar to SHK1 protein kinase, mRNA sequence.    50  9e-05  2
AC020632|AC020632.16 Homo sapiens 3 BAC RP11-333H9 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    44  0.028  6
AP001777|AP001777.2 Homo sapiens genomic DNA, chromosome 18q22 clone:RP11-716B20, WORKING DRAFT SEQUENCE, 22 unordered pieces.    50  0.094  2
AC022275|AC022275.17 Homo sapiens chromosome 3 clone RP11-48F15, WORKING DRAFT SEQUENCE, 7 unordered pieces.    44  0.18  4
AC087534|AC087534.8 Homo sapiens chromosome 18, clone RP11-716B20, complete sequence.    50  0.27  3
AC128253|AC128253.3 Rattus norvegicus clone CH230-468I11, WORKING DRAFT SEQUENCE, 2 unordered pieces.    38  0.31  2
AQ688945|AQ688945.1 nbxb0078L09r CUGI Rice BAC Library Oryza sativa genomic clone nbxb0078L09r, genomic survey sequence.    46  0.50  1
AC105221|AC105221.3 Homo sapiens chromosome 18, clone RP11-480N3, complete sequence.    50  0.82  3
AL928607|AL928607.8 Mouse DNA sequence from clone RP23-326J18 on chromosome 2.    36  0.85  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RB7) RecName: Full=Dual specificity protein kinase shkA;    ...   223   3e-57
(Q54Y55) RecName: Full=Dual specificity protein kinase shkC;    ...   138   1e-31
(Q54IP4) RecName: Full=Dual specificity protein kinase shkB;    ...    91   3e-17
AC012679_7(AC012679|pid:none) Arabidopsis thaliana chromosome 1 ...    82   1e-14
EF014094_1(EF014094|pid:none) Synthetic construct HA-tagged prot...    82   1e-14
AC139526_35(AC139526|pid:none) Medicago truncatula clone mth2-9b...    82   1e-14
AM435166_2(AM435166|pid:none) Vitis vinifera contig VV78X053282....    81   2e-14
EF014090_1(EF014090|pid:none) Synthetic construct flag-tagged pr...    81   2e-14
AY059769_1(AY059769|pid:none) Arabidopsis thaliana putative prot...    80   3e-14
AC012563_26(AC012563|pid:none) Arabidopsis thaliana chromosome 1...    80   3e-14

 psg: 0.66  gvh: 0.44  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for AFA156 is cyt

>AFA156F.Seq
TGTTGGCCTACTGGNATTTTTATTTTTTTTCAAATATTTGTTTAACTCATATAAAGTGAG
AAAGACAAGTGTACATAAAAACTTGATCTTTTTTATTTTATATCACACACATACATAATA
AAAAACTATGGCAACTCAACAACAACAACAACAACAACAACAACAACAACAGCAAATTAA
GGCTAGAAAAGATATACAAATTCAACAAGCACAATCTGCTTCTGATATATTAGGACCACC
AGAAATTTCAGAAACTGAAATTACAACAGAATCAATACTTGGTGATGGAAGTTTTGGTAC
TGTTTATAAAGGTCGTTGTAGATTAAAGGATGTAGCAGTAAAGGTTATGTTAAAACAAGT
CGATCAAAAGACTTTAACTGATTTCAGAAAGGAAGTTGCAATTATGAGTAAAATTTTTCA
TCCAAATATTGTACTTTTTTTAGGTGCATGTACATCTACACCAGGTAAACTTATGATTTG
TACAGAATTAATGAAAGGTAATCTTGAATCATTATTATTAGACCCAATGGTTAAACTCCC
ATTGATCACTCGTATGAGAATGGCAAAAGATGCAGCTTTAGGTGTTTTATGGTTACATTC
TTCA----------