>CHR320 (CHR320Q) /CSM/CH/CHR3-A/CHR320Q.Seq.d/
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGNGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTAGAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAXXXXXXXXX
XTTCTGGTGATCCAGTTGTTAGACCATTATTCTTTGAATATCCATCAGATCCAAATACTT
TTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAGTTCTCACTC
AAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGGTAATG
GTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGTAATCA
ACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGTTACAC
CAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATTGATTA
TTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTGGATGATGGTGAATCAA
TTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTCACCAT
CTTCATCTGCCTACAAATTACAATCAACCATTTTCAATAACAATTATAATGGTACCGCTT
CTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCATTGTTA
ATGGTAGCCCAATCAATTCATTTAATGCTGTTTCTGATTCAACTCTCTCTGTTTCAAATT
TACAACTTGCTTTAGATGAATCCTTTGAAGTTGATTTTGTATTATATTAAAAATTATCAA
CAAATAAAATTAAAATTAGTT

svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPXDCSFNGLIYSNYYLELSXSFTESNPNIYGL---

---SGDPVVRPLFFEYPSDPNTFAIDQQFLVGTGLMVSPVLTQGATTVNAYFPNDIWYEY
GNGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLPFE
LIIALDSSLQATGQLFLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTIFNNNYNG
TASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSDSTLSVSNLQLALDESFEVDFVLY*k
lstnkikis

Frame A:
svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPXDCSFNGLIYSNYYLELSXSFTESNPNIYGL---

---fw*ssc*tiil*isirskyfcn*stifswyrfngitsshsrcyhsecllpk*ylv*i
w*wfigsiswypsnfkcsircnqrsyaww*yhsnstnllicyts*wysnyh*nl*nfti*
idyclgffitsnwsiilg*w*insnlc***ilfhsircclltificlqitinhfq*ql*w
yrffnh*fypnlrfaisstshc*w*pnqfi*ccf*fnslcfkfttcfr*il*s*fciilk
iink*n*n*

Frame B:
vc*kyklklkkk*nfclh*lfflllyyfxlldhyqhhyvniqviqlkxlqkqimvmrqhl
tlfqqvhmvtixnn*ifn*llklvkflxlxlltqiikdgkshqllin*lxkixiqlii*l
nlqiihlvlqqlvfqlxkfyliqlnqxivhlmv*fiqiii*n*vhhsqrviqifmv---

---SGDPVVRPLFFEYPSDPNTFAIDQQFLVGTGLMVSPVLTQGATTVNAYFPNDIWYEY
GNGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLPFE
LIIALDSSLQATGQLFLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTIFNNNYNG
TASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSDSTLSVSNLQLALDESFEVDFVLY*k
lstnkikis

Frame C:
cvknin*n*kknetfvfinyffcyciilxcwiiisiim*isrlfnsrxyknk*wl*gnt*
pyfsrsiw*ryxtikfsinf*n*snfxsxny*pk*skmgsptnc*siswxksxfn*lyn*
iyk*siwfcsnsyfnwxsfi*ynstkxlfi*wfnlfkllfrikxiihre*skylwf---

---lviqlldhyslnihqiqillqlinnf*lvqv*wyhqfslkvlpq*mltsqmisgmnm
vmvhwfnqlvpikl*mlhsm*stficvvvisfqlnqpphmlhqlmvfqlplkslelyhln
*llpwilhykqlvnyswmmvnqfkpmliintlsfnsmlsphhlhlptnynqpfsitiimv
pll*slilskstvrhqfnksllmvaqsihlmlfliqlslfqiynll*mnplklilyyikn
yqqiklklv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR320 (CHR320Q) /CSM/CH/CHR3-A/CHR320Q.Seq.d/                       2313   0.0
CHR858 (CHR858Q) /CSM/CH/CHR8-C/CHR858Q.Seq.d/                       1445   0.0
AHA841 (AHA841Q) /CSM/AH/AHA8-B/AHA841Q.Seq.d/                       1443   0.0
SHJ811 (SHJ811Q) /CSM/SH/SHJ8-A/SHJ811Q.Seq.d/                       1427   0.0
SHG509 (SHG509Q) /CSM/SH/SHG5-A/SHG509Q.Seq.d/                       1419   0.0
AHR217 (AHR217Q) /CSM/AH/AHR2-A/AHR217Q.Seq.d/                       1415   0.0
CHM276 (CHM276Q) /CSM/CH/CHM2-D/CHM276Q.Seq.d/                       1409   0.0
AHM502 (AHM502Q) /CSM/AH/AHM5-A/AHM502Q.Seq.d/                       1409   0.0
SHJ667 (SHJ667Q) /CSM/SH/SHJ6-C/SHJ667Q.Seq.d/                       1405   0.0
AHF873 (AHF873Q) /CSM/AH/AHF8-D/AHF873Q.Seq.d/                       1403   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    40   0.012  11
CR962454|CR962454.1 mth4-17M20RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula, genomic survey sequence.    36   0.16   3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    42   0.16   16
AE014841|AE014841.1 Plasmodium falciparum 3D7 chromosome 11 section 6 of 8 of the complete sequence.    34   0.17   11
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   0.19   13
AX344560|AX344560.1 Sequence 11 from Patent WO0200932.             34   0.24   9
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    34   0.26   10
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   0.36   14
CG939950|CG939950.1 MBEMM28TF mth2 Medicago truncatula genomic clone 3E7, genomic survey sequence.    36   0.37   3
AE014821|AE014821.1 Plasmodium falciparum 3D7 chromosome 14 section 6 of 13 of the complete sequence.    36   0.38   10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF118226_1(AF118226|pid:none) Hordeum vulgare high pI alpha-gluc...   146   1e-33
AB000967_1(AB000967|pid:none) Coturnix japonica GAAI mRNA for ac...   146   2e-33
(Q43763) RecName: Full=Alpha-glucosidase;          EC=3.2.1.20; ...   143   1e-32
AB057452_1(AB057452|pid:none) Physcomitrella patens subsp. paten...   140   7e-32
AF016833_1(AF016833|pid:none) Homo sapiens maltase-glucoamylase ...   138   4e-31
(O43451) RecName: Full=Maltase-glucoamylase, intestinal; Include...   136   1e-30
AB006754_1(AB006754|pid:none) Coturnix japonica GAAII mRNA for a...   135   3e-30
(Q653V7) RecName: Full=Probable alpha-glucosidase Os06g0675700; ...   134   7e-30
EU955937_1(EU955937|pid:none) Zea mays clone 1553282 unknown mRNA.    134   9e-30
AM430903_1(AM430903|pid:none) Vitis vinifera contig VV78X052784....   132   3e-29

 psg: 1.02  gvh: 0.72  alm: 0.40  top: 0.27  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for CHR320 is exc

>CHR320F.Seq
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGNGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTAGAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTANNNNNNNNN
N

>CHR320Z.Seq
NNNNNNNNNNTTCTGGTGATCCAGTTGTTAGACCATTATTCTTTGAATATCCATCAGATC
CAAATACTTTTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAG
TTCTCACTCAAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAAT
ATGGTAATGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCG
ATGTAATCAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCAT
ATGTTACACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTG
AATTGATTATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTGGATGATG
GTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCT
CCTCACCATCTTCATCTGCCTACAAATTACAATCAACCATTTTCAATAACAATTATAATG
GTACCGCTTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAG
TCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCTGATTCAACTCTCTCTG
TTTCAAATTTACAACTTGCTTTAGATGAATCCTTTGAAGTTGATTTTGTATTATATTAAA
AATTATCAACAAATAAAATTAAAATTAGTT

>CHR320P.Seq
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGNGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTAGAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTA---------
-TTCTGGTGATCCAGTTGTTAGACCATTATTCTTTGAATATCCATCAGATCCAAATACTT
TTGCAATTGATCAACAATTTTTAGTTGGTACAGGTTTAATGGTATCACCAGTTCTCACTC
AAGGTGCTACCACAGTGAATGCTTACTTCCCAAATGATATCTGGTATGAATATGGTAATG
GTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCCATTCGATGTAATCA
ACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTCCTCATATGTTACAC
CAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACCATTTGAATTGATTA
TTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTGGATGATGGTGAATCAA
TTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGTTGTCTCCTCACCAT
CTTCATCTGCCTACAAATTACAATCAACCATTTTCAATAACAATTATAATGGTACCGCTT
CTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCAACAAGTCATTGTTA
ATGGTAGCCCAATCAATTCATTTAATGCTGTTTCTGATTCAACTCTCTCTGTTTCAAATT
TACAACTTGCTTTAGATGAATCCTTTGAAGTTGATTTTGTATTATATTAAAAATTATCAA
CAAATAAAATTAAAATTAGTT