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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHR274
Library CH
(Link to library)
Clone ID CHR274
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U11587-1|Contig-U13150-1
Original site URL
Representative seq. ID CHR274P
(Link to Original site)
Representative DNA sequence 
>CHR274 (CHR274Q) /CSM/CH/CHR2-D/CHR274Q.Seq.d/
GTAATAATAATTTTAAAAAATCAAATAGTAATGAAACTGATGGTAATAATAGTGTAAATA
TAACAAAAGATGGTCATAATTCAGTTTCACCATTAACAAAAACAACAAGAGCAACTGAAA
ATACATTAAATAGTAGTAGTGGTAATGTAAATAATACAAATACTGATGGTACTGATGGTG
GTGTAAATAATACAAATACAAATACTGATAGTGGTACAGAACCACCTCCACCACAAATCT
CTGAAGAACCAAATGTACCAAAAGAAGTATTATGGAGGGATGAAGATGTTACTTCTGATG
GTAGTGATATTGCTGATGATGATTTAGATAAAATTGATTTAGAATCAGTTACTTCTCCAG
AAAATACTACAACAACAACAACAACTACAACAACAACTACAACAAGTGAACCAAAAATTA
TTAGACCTGAATTTGATGAAAATTCAATAACTTTTGAAAATTTATCAGAAACACTCTCAA
ATTTCATTGATAATTTCGGGTCAGAATCAACTAAAGATAAATCTAAAAAACAATATATTT
CATTTGAAATTGAACCAAACTTTAACAAGGTTAAATTACCAATCTCTTATAATAATAGAA
ATTTAGATXXXXXXXXXXGGTCCTAGAATTACTTTTCATCATATAGATTGTCCACTATGT
AAGAAACAAATGAAATCTCATCCTGCAATTAATCATATTTTAGAACCAATCTTAAAGAAT
AAAGCAATCATTCAATCAAAAGCAATGGAAAGATTATCATATGAAGGTTTAGCAAAATGT
AAAGAGATTCAAGATAAAAACTCAAAGTGGTATCAAGATCCTGAAAATTACTCAATGGAT
AGATATGTGTACTATAGTTGCTTTAAATGTAAAGGTTTTTATTTTGCTGGTCAAGCACGT
TGTGATGAAGGTGTAGAAGGTGGTTACAAAGATGAAGACTTAATTTGTGGTTCTTGTAGG
GGAAGTGATGGAAAAGAATGTAAAGTACATGGTTGGGACTATATCATATGGAAATGTCAG
TTTTGTTGTTCAATAGCTCAATGGTATTGCTGGAGCAAGACGCACTTCTGTTCCCAATGC
CACGCTATTCAAAATGGCGGAAAGTACCTCAACAAAATACCCAAAACCGAAATCCCTCGA
TGTGAAGGTCCTGATAAATGTCCCCTTAAGGTTGATCATCCTCATGTCGAAGAATTTATT
TTAGGTTGTACTCTTTGTAAAAATTTAAAAGAATTTTAATCAAATCAATCAAGACCACTT
TAATCCTTTGTAAATAATATCGTTTTCAAGAACAAAAAAGTAAAAAACTTTGTAAATAAA
TAT
sequence update 2002.10.25
Translated Amino Acid sequence 
NNNFKKSNSNETDGNNSVNITKDGHNSVSPLTKTTRATENTLNSSSGNVNNTNTDGTDGG
VNNTNTNTDSGTEPPPPQISEEPNVPKEVLWRDEDVTSDGSDIADDDLDKIDLESVTSPE
NTTTTTTTTTTTTTSEPKIIRPEFDENSITFENLSETLSNFIDNFGSESTKDKSKKQYIS
FEIEPNFNKVKLPISYNNRNLD---

---GPRITFHHIDCPLCKKQMKSHPAINHILEPILKNKAIIQSKAMERLSYEGLAKCKEI
QDKNSKWYQDPENYSMDRYVYYSCFKCKGFYFAGQARCDEGVEGGYKDEDLICGSCRGSD
GKECKVHGWDYIIWKCQFCCSIAQWYCWSKTHFCSQCHAIQNGGKYLNKIPKTEIPRCEG
PDKCPLKVDHPHVEEFILGCTLCKNLKEF*snqsrpl*sfvnnivfknkkvknfvnky
Translated Amino Acid sequence (All Frames) 
Frame A:
viiilknqivmklmviiv*i*qkmviiqfhh*qkqqeqlkih*ivvvvm*iiqilmvlmv
v*iiqiqilivvqnhlhhkslknqmyqkkyyggmkmlllmvvillmmi*ikli*nqlllq
kilqqqqqlqqqlqqvnqklldlnlmkiq*llkiyqkhsqisliisgqnqlkinlknnif
hlklnqtltrlnyqsliiiei*---

---GPRITFHHIDCPLCKKQMKSHPAINHILEPILKNKAIIQSKAMERLSYEGLAKCKEI
QDKNSKWYQDPENYSMDRYVYYSCFKCKGFYFAGQARCDEGVEGGYKDEDLICGSCRGSD
GKECKVHGWDYIIWKCQFCCSIAQWYCWSKTHFCSQCHAIQNGGKYLNKIPKTEIPRCEG
PDKCPLKVDHPHVEEFILGCTLCKNLKEF*snqsrpl*sfvnnivfknkkvknfvnky

Frame B:
***f*kik***n*w***ckynkrws*fsftinknnksn*kyik***w*ck*yky*wy*ww
ck*ykyky**wyrttsttnl*rtkctkrsimeg*rcyf*w**yc***fr*n*frisyfsr
kyynnnnnynnnynk*tkny*t*i**kfnnf*kfirntlkfh**frvrin*r*i*ktiyf
i*n*tkl*qg*itnll***kfr---

---vlellfii*ivhyvrnk*nlilqliif*nqs*rikqsfnqkqwkdyhmkv*qnvkrf
kiktqsgikilkitqwidmctivalnvkvfillvkhvvmkv*kvvtkmkt*fvvlvgevm
eknvkymvgtisygnvsfvvq*lngiagarrtsvpnatlfkmaeststkypkpksldvkv
linvplrliilmsknlf*vvlfvki*knfnqinqdhfnpl*iisfsrtkk*ktl*in

Frame C:
NNNFKKSNSNETDGNNSVNITKDGHNSVSPLTKTTRATENTLNSSSGNVNNTNTDGTDGG
VNNTNTNTDSGTEPPPPQISEEPNVPKEVLWRDEDVTSDGSDIADDDLDKIDLESVTSPE
NTTTTTTTTTTTTTSEPKIIRPEFDENSITFENLSETLSNFIDNFGSESTKDKSKKQYIS
FEIEPNFNKVKLPISYNNRNLD---

---s*nyfssyrlstm*etneisscn*syfrtnlke*snhsiksngkiii*rfskm*rds
r*klkvvsrs*kllng*icvl*ll*m*rflfcwsstl**rcrrwlqr*rlnlwfl*gk*w
krm*stwlglyhmemsvllfnssmvlleqdallfpmpryskwrkvpqqntqnrnpsm*rs
**msp*g*ssscrriyfrlysl*kfkriliksikttlilck*yrfqeqkskklck*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR274 (CHR274Q) /CSM/CH/CHR2-D/CHR274Q.Seq.d/                       1701   0.0
SHG322 (SHG322Q) /CSM/SH/SHG3-A/SHG322Q.Seq.d/                        728   0.0
CHJ854 (CHJ854Q) /CSM/CH/CHJ8-C/CHJ854Q.Seq.d/                        728   0.0
CFF246 (CFF246Q) /CSM/CF/CFF2-B/CFF246Q.Seq.d/                        728   0.0
CFG704 (CFG704Q) /CSM/CF/CFG7-A/CFG704Q.Seq.d/                        714   0.0
CFH544 (CFH544Q) /CSM/CF/CFH5-B/CFH544Q.Seq.d/                        700   0.0
SLC336 (SLC336Q) /CSM/SL/SLC3-B/SLC336Q.Seq.d/                        666   0.0
AFO132 (AFO132Q) /CSM/AF/AFO1-B/AFO132Q.Seq.d/                        351   2e-95
AFB548 (AFB548Q) /CSM/AF/AFB5-B/AFB548Q.Seq.d/                        299   6e-80
AFK642 (AFK642Q) /CSM/AF/AFK6-B/AFK642Q.Seq.d/                        285   9e-76
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    42   1e-05  12
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    34   2e-04  11
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   5e-04  18
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    40   0.011  13
BX323828|BX323828.7 Zebrafish DNA sequence from clone DKEY-102M7 in linkage group 14.    52   0.030  1
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    36   0.031  11
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    32   0.037  16
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   0.039  14
CR354589|CR354589.15 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-93L13.    44   0.040  6
BX927161|BX927161.7 Zebrafish DNA sequence from clone DKEY-181G6 in linkage group 19.    40   0.065  6
dna update 2005.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB172863_1(AB172863|pid:none) Macaca fascicularis brain cDNA clo...   184   6e-45
(O75592) RecName: Full=Probable E3 ubiquitin-protein ligase MYCB...   184   6e-45
AF075587_1(AF075587|pid:none) Homo sapiens protein associated wi...   184   6e-45
AL359257_2(AL359257|pid:none) Human DNA sequence from clone RP11...   184   6e-45
BC037971_1(BC037971|pid:none) Homo sapiens MYC binding protein 2...   184   6e-45
AY325887_1(AY325887|pid:none) Mus musculus highwire (Phr1) mRNA,...   184   8e-45
AB172733_1(AB172733|pid:none) Macaca fascicularis brain cDNA clo...   182   3e-44
BC059257_1(BC059257|pid:none) Mus musculus MYC binding protein 2...   181   5e-44
AF083244_1(AF083244|pid:none) Homo sapiens HSPC026 mRNA, complet...   180   8e-44
AY113376_1(AY113376|pid:none) Drosophila melanogaster LP10764 fu...   174   5e-42
protein update 2009. 4.12
PSORT 

 psg: 0.57  gvh: 0.34  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHR274 is cyt
5' end seq. ID CHR274F
5' end seq. 
>CHR274F.Seq
GTAATAATAATTTTAAAAAATCAAATAGTAATGAAACTGATGGTAATAATAGTGTAAATA
TAACAAAAGATGGTCATAATTCAGTTTCACCATTAACAAAAACAACAAGAGCAACTGAAA
ATACATTAAATAGTAGTAGTGGTAATGTAAATAATACAAATACTGATGGTACTGATGGTG
GTGTAAATAATACAAATACAAATACTGATAGTGGTACAGAACCACCTCCACCACAAATCT
CTGAAGAACCAAATGTACCAAAAGAAGTATTATGGAGGGATGAAGATGTTACTTCTGATG
GTAGTGATATTGCTGATGATGATTTAGATAAAATTGATTTAGAATCAGTTACTTCTCCAG
AAAATACTACAACAACAACAACAACTACAACAACAACTACAACAAGTGAACCAAAAATTA
TTAGACCTGAATTTGATGAAAATTCAATAACTTTTGAAAATTTATCAGAAACACTCTCAA
ATTTCATTGATAATTTCGGGTCAGAATCAACTAAAGATAAATCTAAAAAACAATATATTT
CATTTGAAATTGAACCAAACTTTAACAAGGTTAAATTACCAATCTCTTATAATAATAGAA
ATTTAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID CHR274Z
3' end seq. 
>CHR274Z.Seq
NNNNNNNNNNGGTCCTAGAATTACTTTTCATCATATAGATTGTCCACTATGTAAGAAACA
AATGAAATCTCATCCTGCAATTAATCATATTTTAGAACCAATCTTAAAGAATAAAGCAAT
CATTCAATCAAAAGCAATGGAAAGATTATCATATGAAGGTTTAGCAAAATGTAAAGAGAT
TCAAGATAAAAACTCAAAGTGGTATCAAGATCCTGAAAATTACTCAATGGATAGATATGT
GTACTATAGTTGCTTTAAATGTAAAGGTTTTTATTTTGCTGGTCAAGCACGTTGTGATGA
AGGTGTAGAAGGTGGTTACAAAGATGAAGACTTAATTTGTGGTTCTTGTAGGGGAAGTGA
TGGAAAAGAATGTAAAGTACATGGTTGGGACTATATCATATGGAAATGTCAGTTTTGTTG
TTCAATAGCTCAATGGTATTGCTGGAGCAAGACGCACTTCTGTTCCCAATGCCACGCTAT
TCAAAATGGCGGAAAGTACCTCAACAAAATACCCAAAACCGAAATCCCTCGATGTGAAGG
TCCTGATAAATGTCCCCTTAAGGTTGATCATCCTCATGTCGAAGAATTTATTTTAGGTTG
TACTCTTTGTAAAAATTTAAAAGAATTTTAATCAAATCAATCAAGACCACTTTAATCCTT
TGTAAATAATATCGTTTTCAAGAACAAAAAAGTAAAAAACTTTGTAAATAAATAT
Length of 3' end seq. 715
Connected seq. ID CHR274P
Connected seq. 
>CHR274P.Seq
GTAATAATAATTTTAAAAAATCAAATAGTAATGAAACTGATGGTAATAATAGTGTAAATA
TAACAAAAGATGGTCATAATTCAGTTTCACCATTAACAAAAACAACAAGAGCAACTGAAA
ATACATTAAATAGTAGTAGTGGTAATGTAAATAATACAAATACTGATGGTACTGATGGTG
GTGTAAATAATACAAATACAAATACTGATAGTGGTACAGAACCACCTCCACCACAAATCT
CTGAAGAACCAAATGTACCAAAAGAAGTATTATGGAGGGATGAAGATGTTACTTCTGATG
GTAGTGATATTGCTGATGATGATTTAGATAAAATTGATTTAGAATCAGTTACTTCTCCAG
AAAATACTACAACAACAACAACAACTACAACAACAACTACAACAAGTGAACCAAAAATTA
TTAGACCTGAATTTGATGAAAATTCAATAACTTTTGAAAATTTATCAGAAACACTCTCAA
ATTTCATTGATAATTTCGGGTCAGAATCAACTAAAGATAAATCTAAAAAACAATATATTT
CATTTGAAATTGAACCAAACTTTAACAAGGTTAAATTACCAATCTCTTATAATAATAGAA
ATTTAGAT----------GGTCCTAGAATTACTTTTCATCATATAGATTGTCCACTATGT
AAGAAACAAATGAAATCTCATCCTGCAATTAATCATATTTTAGAACCAATCTTAAAGAAT
AAAGCAATCATTCAATCAAAAGCAATGGAAAGATTATCATATGAAGGTTTAGCAAAATGT
AAAGAGATTCAAGATAAAAACTCAAAGTGGTATCAAGATCCTGAAAATTACTCAATGGAT
AGATATGTGTACTATAGTTGCTTTAAATGTAAAGGTTTTTATTTTGCTGGTCAAGCACGT
TGTGATGAAGGTGTAGAAGGTGGTTACAAAGATGAAGACTTAATTTGTGGTTCTTGTAGG
GGAAGTGATGGAAAAGAATGTAAAGTACATGGTTGGGACTATATCATATGGAAATGTCAG
TTTTGTTGTTCAATAGCTCAATGGTATTGCTGGAGCAAGACGCACTTCTGTTCCCAATGC
CACGCTATTCAAAATGGCGGAAAGTACCTCAACAAAATACCCAAAACCGAAATCCCTCGA
TGTGAAGGTCCTGATAAATGTCCCCTTAAGGTTGATCATCCTCATGTCGAAGAATTTATT
TTAGGTTGTACTCTTTGTAAAAATTTAAAAGAATTTTAATCAAATCAATCAAGACCACTT
TAATCCTTTGTAAATAATATCGTTTTCAAGAACAAAAAAGTAAAAAACTTTGTAAATAAA
TAT
Length of connected seq. 1313
Full length Seq ID -
Full length Seq. -
Length of full length seq. -