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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHR254
Library CH
(Link to library)
Clone ID CHR254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11845-1|Contig-U13149-1
Original site URL
Representative seq. ID CHR254P
(Link to Original site)
Representative DNA sequence 
>CHR254 (CHR254Q) /CSM/CH/CHR2-C/CHR254Q.Seq.d/
CNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCNTTTTTTTT
TTNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAANNGGGGCCCCCCCCNTNNNNNAAAAA
XXXXXXXXXXAACTTTAGACATTTTCTCTGTCGATGAAGAAGAAATCGCTAGACAATTGA
CCTTGATGGACTTTGAAATCTTTTCAGCCATTAAATCAACCGAATTGTTGAATCAAAGTT
GGAATAAACCAAAACTACGTCACCGTTCACCAAATGTATTGACTTTGATCAATAGATTCA
ATGAGATCTCTCAATGGACTGCAACTTCGATTCTCTCTTATCCAAAGGTTAAGGATAGAG
CTAGAATCATGGCTAAGTTCATAAAGATCGCTGAATATTGTATGAGACATTTAAATAATT
TCAATACTTCAATGGCAATTCTTTCAGGTTTAAACGCTTCCTCTGTTCATAGATTAAAAT
TCACCAAAGAAGAGTTACCAAAACATACTCAACAAGTTTACACAGAGTTACAATTTCATC
TAAGTTCAGCTCAAGCTTACACCANAGTATCGTGCTCCCTTGGCAAAAGCNCATCCACCT
NGTTTACCTTATCTTGGTGNNTATTTGGCAGATTTNACTTTCTTTGAAGAAGGTAATCNT
GATTTCATTCAAGGTTTCATCAATTTTGGTAAAAGNNAGTTAATCTATGGNNCAATTTCA
AACGTTCAAAGTTTTCAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
XKKKKKKKKKKKKKPPPXFFXXXKKKKKKKKXGAPPXXXK---

---TLDIFSVDEEEIARQLTLMDFEIFSAIKSTELLNQSWNKPKLRHRSPNVLTLINRFN
EISQWTATSILSYPKVKDRARIMAKFIKIAEYCMRHLNNFNTSMAILSGLNASSVHRLKF
TKEELPKHTQQVYTELQFHLSSAQAYTXVSCSLGKSXSTXFTLSWXXFGRFXFL*rr*s*
fhsrfhqfw*kxvnlwxnfkrskfsk
Translated Amino Acid sequence (All Frames) 
Frame A:
XKKKKKKKKKKKKKPPPXFFXXXKKKKKKKKXGAPPXXXK---

---nfrhflcr*rrnr*tidldgl*nlfsh*inriveskle*tkttspftkcidfdq*iq
*dlsmdcnfdsllskg*g*s*nhg*vhkdr*ilyetfk*fqyfngnsfrfkrflcs*iki
hqrrvtktystslhrvtisskfssslhxsivlpwqkxihlvylilvxiwqixlslkkvix
isfkvssilvkxs*smxqfqtfkvfk

Frame B:
xkkkkkkkkkkkknpppfffxxkkkkkkkkkxgpppxxk---

---TLDIFSVDEEEIARQLTLMDFEIFSAIKSTELLNQSWNKPKLRHRSPNVLTLINRFN
EISQWTATSILSYPKVKDRARIMAKFIKIAEYCMRHLNNFNTSMAILSGLNASSVHRLKF
TKEELPKHTQQVYTELQFHLSSAQAYTXVSCSLGKSXSTXFTLSWXXFGRFXFL*rr*s*
fhsrfhqfw*kxvnlwxnfkrskfsk

Frame C:
xkkkkkkkkkkkktppxfffxxkkkkkkkkxxgppxxxk---

---l*tfslsmkkksldn*p*wtlksfqplnqpnc*ikvginqnyvtvhqmy*l*sidsm
rslnglqlrfsliqrlrieleswlss*rslniv*di*iisilqwqffqv*tlplfid*ns
pkksyqnilnkftqsynfi*vqlkltpxyraplakahppxlpylgxyladxtffeegnxd
fiqgfinfgkxxliygxisnvqsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR254 (CHR254Q) /CSM/CH/CHR2-C/CHR254Q.Seq.d/                       1144   0.0
CHM362 (CHM362Q) /CSM/CH/CHM3-C/CHM362Q.Seq.d/                       1043   0.0
AHK701 (AHK701Q) /CSM/AH/AHK7-A/AHK701Q.Seq.d/                        718   0.0
VHF325 (VHF325Q) /CSM/VH/VHF3-B/VHF325Q.Seq.d/                         46   8e-04
VHB802 (VHB802Q) /CSM/VH/VHB8-A/VHB802Q.Seq.d/                         46   8e-04
SSL484 (SSL484Q) /CSM/SS/SSL4-D/SSL484Q.Seq.d/                         46   8e-04
SFI279 (SFI279Q) /CSM/SF/SFI2-D/SFI279Q.Seq.d/                         46   8e-04
CHJ817 (CHJ817Q) /CSM/CH/CHJ8-A/CHJ817Q.Seq.d/                         46   8e-04
CFJ525 (CFJ525Q) /CSM/CF/CFJ5-B/CFJ525Q.Seq.d/                         46   8e-04
CFA234 (CFA234Q) /CSM/CF/CFA2-B/CFA234Q.Seq.d/                         46   8e-04
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY160099|AY160099.1 Dictyostelium discoideum nucleotide exchange factor RasGEF J (gefJ) gene, complete cds.   854   0.0    2
AY160098|AY160098.1 Dictyostelium discoideum nucleotide exchange factor RasGEF I (gefI) gene, complete cds.   343   0.0    3
AF474377|AF474377.1 Dictyostelium discoideum Ras GTP exchange factor K (gefK) mRNA, complete cds.    46   3e-11  3
AY160100|AY160100.1 Dictyostelium discoideum nucleotide exchange factor RasGEF K (gefK) gene, complete cds.    46   5e-11  3
BQ546593|BQ546593.1 EST630220 TpMugugaSh01 Theileria parva cDNA clone TPFAX53, mRNA sequence.    50   0.066  1
U53884|U53884.1 Dictyostelium discoideum aimless RasGEF (aleA) mRNA, complete cds.    38   1.3    3
AY160090|AY160090.1 Dictyostelium discoideum nucleotide exchange factor RasGEF A (gefA) gene, complete cds.    38   1.5    3
AW013549|AW013549.1 pc096A Winter flounder pyloric caeca Pleuronectes americanus cDNA clone pc096A 5', mRNA sequence.    44   4.1    1
U00055|U00055.2 Caenorhabditis elegans cosmid R02F2, complete sequence.    44   4.1    1
dna update 2003. 4.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY160099_1(AY160099|pid:none) Dictyostelium discoideum nucleotid...   286   4e-89
AY160098_1(AY160098|pid:none) Dictyostelium discoideum nucleotid...   268   8e-84
U53884_1(U53884|pid:none) Dictyostelium discoideum aimless RasGE...   162   7e-39
AY160090_1(AY160090|pid:none) Dictyostelium discoideum nucleotid...   162   7e-39
AY160100_1(AY160100|pid:none) Dictyostelium discoideum nucleotid...   129   3e-34
AF474377_1(AF474377|pid:none) Dictyostelium discoideum Ras GTP e...   129   3e-34
AC115592_17(AC115592|pid:none) Dictyostelium discoideum chromoso...   115   2e-30
AF275723_1(AF275723|pid:none) Dictyostelium discoideum Ras guani...   108   4e-28
AC115682_8(AC115682|pid:none) Dictyostelium discoideum chromosom...   115   1e-24
AC116987_20(AC116987|pid:none) Dictyostelium discoideum chromoso...   115   1e-24
protein update 2009. 4.12
PSORT 

 psg: 0.64  gvh: 0.24  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 1.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for CHR254 is nuc
5' end seq. ID CHR254F
5' end seq. 
>CHR254F.Seq
CNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCNTTTTTTTT
TTNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAANNGGGGCCCCCCCCNTNNNNNAAAAA
NNNNNNNNNN
Length of 5' end seq. 130
3' end seq. ID CHR254Z
3' end seq. 
>CHR254Z.Seq
NNNNNNNNNNAACTTTAGACATTTTCTCTGTCGATGAAGAAGAAATCGCTAGACAATTGA
CCTTGATGGACTTTGAAATCTTTTCAGCCATTAAATCAACCGAATTGTTGAATCAAAGTT
GGAATAAACCAAAACTACGTCACCGTTCACCAAATGTATTGACTTTGATCAATAGATTCA
ATGAGATCTCTCAATGGACTGCAACTTCGATTCTCTCTTATCCAAAGGTTAAGGATAGAG
CTAGAATCATGGCTAAGTTCATAAAGATCGCTGAATATTGTATGAGACATTTAAATAATT
TCAATACTTCAATGGCAATTCTTTCAGGTTTAAACGCTTCCTCTGTTCATAGATTAAAAT
TCACCAAAGAAGAGTTACCAAAACATACTCAACAAGTTTACACAGAGTTACAATTTCATC
TAAGTTCAGCTCAAGCTTACACCANAGTATCGTGCTCCCTTGGCAAAAGCNCATCCACCT
NGTTTACCTTATCTTGGTGNNTATTTGGCAGATTTNACTTTCTTTGAAGAAGGTAATCNT
GATTTCATTCAAGGTTTCATCAATTTTGGTAAAAGNNAGTTAATCTATGGNNCAATTTCA
AACGTTCAAAGTTTTCAAAA
Length of 3' end seq. 620
Connected seq. ID CHR254P
Connected seq. 
>CHR254P.Seq
CNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCNTTTTTTTT
TTNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAANNGGGGCCCCCCCCNTNNNNNAAAAA
----------AACTTTAGACATTTTCTCTGTCGATGAAGAAGAAATCGCTAGACAATTGA
CCTTGATGGACTTTGAAATCTTTTCAGCCATTAAATCAACCGAATTGTTGAATCAAAGTT
GGAATAAACCAAAACTACGTCACCGTTCACCAAATGTATTGACTTTGATCAATAGATTCA
ATGAGATCTCTCAATGGACTGCAACTTCGATTCTCTCTTATCCAAAGGTTAAGGATAGAG
CTAGAATCATGGCTAAGTTCATAAAGATCGCTGAATATTGTATGAGACATTTAAATAATT
TCAATACTTCAATGGCAATTCTTTCAGGTTTAAACGCTTCCTCTGTTCATAGATTAAAAT
TCACCAAAGAAGAGTTACCAAAACATACTCAACAAGTTTACACAGAGTTACAATTTCATC
TAAGTTCAGCTCAAGCTTACACCANAGTATCGTGCTCCCTTGGCAAAAGCNCATCCACCT
NGTTTACCTTATCTTGGTGNNTATTTGGCAGATTTNACTTTCTTTGAAGAAGGTAATCNT
GATTTCATTCAAGGTTTCATCAATTTTGGTAAAAGNNAGTTAATCTATGGNNCAATTTCA
AACGTTCAAAGTTTTCAAAA
Length of connected seq. 730
Full length Seq ID -
Full length Seq. -
Length of full length seq. -