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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH403
Library AF
(Link to library)
Clone ID AFH403
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16463-1
Original site URL
Representative seq. ID AFH403P
(Link to Original site)
Representative DNA sequence 
>AFH403 (AFH403Q) /CSM/AF/AFH4-A/AFH403Q.Seq.d/
ACTGTTGGCCTACTGGNATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATA
TGTCAGCTCCAGTTTTAACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAA
TGACCGCAGGTGTTAATGGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTG
CTCATTTCGATCGTGAACGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCC
ATGGTTATTTCGAAGTTACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAA
ATAAAGTTGGTAAGAGAACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAG
GTTCATCCGATTCTGAAAGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAG
GTAACTTTGATATGGTCGGTAATAATACTCCAGTTTTCTTTATTCGNGATCCATCAAAAT
TCCCAGATTTCATTCATACACAXXXXXXXXXXCTTTGCTGAAGTTGAACAAGCTGCCTTC
TCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGT
CTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCA
GTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATG
GCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCA
GAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAA
CCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCT
GAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATC
AAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCT
CGTTTATGTAAGGGTCTTGGTATTGATGTTAANATGTCATCAAATTCGCTGCAGATCAAA
CTNTAATCAAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
cwptgiyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLA
HFDRERIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKG
SSDSERDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHT---

---FAEVEQAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVK
GGVKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDD
FVQPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGI
DVXMSSNSLQIKL*s
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxyilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linl
lisivnvsqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkk
vhpilkeiqevslsnfilkkvtliwsviilqfslfxihqnsqisfih---

---lc*s*tsclltiphgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrk
gwc*klpt*wfhgcqw*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*r
lcstr*fi*thv*rcqisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gswy
*c*xvikfaadqtxin

Frame B:
llaywxifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tc
sfrs*typrtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlhpflycww*kr
firf*krskrfrcqily*rr*l*ygr**yssflysxsikiprfhsyt---

---FAEVEQAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVK
GGVKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDD
FVQPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGI
DVXMSSNSLQIKL*s

Frame C:
cwptgiyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLA
HFDRERIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKG
SSDSERDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHT---

---llklnklpshhptwfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*r
vvlkttnvmvswlsmvmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmtt
lfnqviyidsclkmpnlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvl
mlxchqircrsnxnq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH403 (AFH403Q) /CSM/AF/AFH4-A/AFH403Q.Seq.d/                       1966   0.0
CFA669 (CFA669Q) /CSM/CF/CFA6-C/CFA669Q.Seq.d/                       1122   0.0
AFO414 (AFO414Q) /CSM/AF/AFO4-A/AFO414Q.Seq.d/                       1122   0.0
CFI634 (CFI634Q) /CSM/CF/CFI6-B/CFI634Q.Seq.d/                       1112   0.0
AFI691 (AFI691Q) /CSM/AF/AFI6-D/AFI691Q.Seq.d/                       1112   0.0
AFE708 (AFE708Q) /CSM/AF/AFE7-A/AFE708Q.Seq.d/                       1112   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1110   0.0
AFE574 (AFE574Q) /CSM/AF/AFE5-D/AFE574Q.Seq.d/                       1110   0.0
AFE294 (AFE294Q) /CSM/AF/AFE2-D/AFE294Q.Seq.d/                       1110   0.0
AFE110 (AFE110Q) /CSM/AF/AFE1-A/AFE110Q.Seq.d/                       1110   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1070   0.0    2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1070   0.0    2
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111   9e-59  6
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.          101   1e-26  2
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    94   3e-24  2
AR375090|AR375090.1 Sequence 96 from patent US 6605709.            76   4e-24  3
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76   2e-23  3
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76   2e-23  3
U40704|U40704.1 Candida albicans catalase (Cat) gene, complete cds.    86   7e-22  2
AY378080|AY378080.1 Tetrahymena thermophila putative catalase (CAT) mRNA, complete cds.    78   2e-20  4
dna update 2004. 5.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   381   e-104
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   246   1e-63
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   228   3e-58
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   228   3e-58
(Q9XZD5) RecName: Full=Peroxisomal catalase;          EC=1.11.1....   227   6e-58
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   226   1e-57
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   226   1e-57
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   225   2e-57
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   218   2e-55
(P90682) RecName: Full=Catalase;          EC=1.11.1.6;  &Y10611_...   217   6e-55
protein update 2009. 4.21
PSORT 

 psg: 0.89  gvh: 0.57  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFH403 is nuc
5' end seq. ID AFH403F
5' end seq. 
>AFH403F.Seq
ACTGTTGGCCTACTGGNATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATA
TGTCAGCTCCAGTTTTAACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAA
TGACCGCAGGTGTTAATGGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTG
CTCATTTCGATCGTGAACGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCC
ATGGTTATTTCGAAGTTACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAA
ATAAAGTTGGTAAGAGAACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAG
GTTCATCCGATTCTGAAAGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAG
GTAACTTTGATATGGTCGGTAATAATACTCCAGTTTTCTTTATTCGNGATCCATCAAAAT
TCCCAGATTTCATTCATACACA----------
Length of 5' end seq. 502
3' end seq. ID AFH403Z
3' end seq. 
>AFH403Z.Seq
----------CTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAG
GTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTC
ATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAA
AGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTA
AAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTC
AACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACG
ACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCG
TCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCG
TTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTA
TTGATGTTAANATGTCATCAAATTCGCTGCAGATCAAACTNTAATCAAT
Length of 3' end seq. 579
Connected seq. ID AFH403P
Connected seq. 
>AFH403P.Seq
ACTGTTGGCCTACTGGNATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATA
TGTCAGCTCCAGTTTTAACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAA
TGACCGCAGGTGTTAATGGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTG
CTCATTTCGATCGTGAACGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCC
ATGGTTATTTCGAAGTTACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAA
ATAAAGTTGGTAAGAGAACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAG
GTTCATCCGATTCTGAAAGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAG
GTAACTTTGATATGGTCGGTAATAATACTCCAGTTTTCTTTATTCGNGATCCATCAAAAT
TCCCAGATTTCATTCATACACA----------CTTTGCTGAAGTTGAACAAGCTGCCTTC
TCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGT
CTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCA
GTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATG
GCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCA
GAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAA
CCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCT
GAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATC
AAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCT
CGTTTATGTAAGGGTCTTGGTATTGATGTTAANATGTCATCAAATTCGCTGCAGATCAAA
CTNTAATCAAT
Length of connected seq. 1081
Full length Seq ID -
Full length Seq. -
Length of full length seq. -