>CHR147 (CHR147Q) /CSM/CH/CHR1-B/CHR147Q.Seq.d/
ATAGCAAGAATCATAGACCCGAGTCAATTGAAGTTGACAAAGGTTTACAAACTGTAAATC
GTTTAATGGATTTAACTGATGCTGTCTTTAGAATGCATAATTCAATCGTATCAGCAACAC
CTTATAATGATTGTAAAACTTCTGCTTTCATTCCATTAGTTTTAGAATCTTATGCTATCT
ATATTTTAGTTGTACATTTTTTAACAAATTTAATTGATAAGGTTGATAATAAAGAAGTTA
TGGATTTTTCAATTAAAAGATTTTATGGTCAATATCAAACATTGAGAAACTTTTTTTTTA
AATGCAAGTGCAATTACAAGTGTATCTAGTGTTATTGCAGTACCAAATTTACCACCAGAT
CCTCCAAAGTTTATTAGAAAAGTTAGAGAGGCACCAAAGGCCAAACCACAGGCAATTCCA
CCACCAGTTGTAAAGGAACCACCAACACCACCACCTGAACAAATTCCAGTTCCAGTACCA
ACACCTGTATTCATTCGTCAAACGGTACCAGTGGTTTATGGCGCACCTTATGGTATGCAA
CAACAACAACAAGATAATAGTATGTGGGGTCAGTTTGGTTCCAATCAACCAXXXXXXXXX
XCTGGTATCAATAATTTGGATGCATTGGCTCAACAAGCTGACGCTGAAAGAATTGAAGCC
GAGAAGAAATATTTAGAGAATGGTTTTGATTTGGATGATGTTGCTGAACGTGAACTATTG
GCTGCCGCTAAAACCATTGAAGATGCTGCAAACTCATTATTGGCCGCAAAATCAAAGAGA
GCTCCAAAGAAGGAGGGTGATTCACCAGATGTTGCTGAGGCAATCCTTGAGGCTGCTATG
GCAATCACTGGTGCTACTTCCACATTGGTTGGTGCCGCCACTTTAGCACAAAGAGAACGT
GTCGAAAAGGGTAGAACAAGTGATGGTGGTCCACTCTATAGAAAGGATCCAACTTGGGCT
GAAGGTTTAATCTCTGCTGCAAAATCTGTTGCCCAAGCCACTAAAGCTTTGGTAGATAAT
GCCAACAAGACAGCATCTGGTCAAGAGATCCCAGGTGGTACAGAAGAAGCTCTCATTGCA
ACTTCGATGGCTGTAACTGCTGCAACTTCTCAATTGGTCTCTGCTTGTAAATCTAAATCT
GATATCAATAGTCCTTCTCAACATAAACTTTCAAATGCTGCCAAATCCGTATCCAATGCA
ACCAATCTTTTAGTGGCTGCAGCCAAAGCTGTCGCTGCTAAAGTCGCCTCTGAAGAGGAT
ATCGATTTCGATAAACTCACTGTCACTGGTTATAAAGTTAAAGAAATGGAACAACAAATG
GCAATTCTCAGATTAGAAAAAGAATTAGAAGTCGCTAGAAAAGGTTTAAGTGTTATTAGA
AAGAAACAATAGA

iariidpsqlkltkvykl*iv*wi*lmlsleciiqsyqqhlimivklllsfh*f*nlmls
if*lyif*qi*lirliikklwifqlkdfmvnikh*ETFFLNASAITSVSSVIAVPNLPPD
PPKFIRKVREAPKAKPQAIPPPVVKEPPTPPPEQIPVPVPTPVFIRQTVPVVYGAPYGMQ
QQQQDNSMWGQFGSNQP---

---GINNLDALAQQADAERIEAEKKYLENGFDLDDVAERELLAAAKTIEDAANSLLAAKS
KRAPKKEGDSPDVAEAILEAAMAITGATSTLVGAATLAQRERVEKGRTSDGGPLYRKDPT
WAEGLISAAKSVAQATKALVDNANKTASGQEIPGGTEEALIATSMAVTAATSQLVSACKS
KSDINSPSQHKLSNAAKSVSNATNLLVAAAKAVAAKVASEEDIDFDKLTVTGYKVKEMEQ
QMAILRLEKELEVARKGLSVIRKKQ*

Frame A:
iariidpsqlkltkvykl*iv*wi*lmlsleciiqsyqqhlimivklllsfh*f*nlmls
if*lyif*qi*lirliikklwifqlkdfmvnikh*ETFFLNASAITSVSSVIAVPNLPPD
PPKFIRKVREAPKAKPQAIPPPVVKEPPTPPPEQIPVPVPTPVFIRQTVPVVYGAPYGMQ
QQQQDNSMWGQFGSNQP---

---lvsiiwmhwlnkltlkelkprrni*rmvliwmmllnvnywlplkplkmlqthywpqn
qrelqrrrvihqmllrqslrllwqslvllphwlvppl*hkenvskrveqvmvvhsieriq
lglkv*sllqnllpkplklw*imptrqhlvkrsqvvqkklslqlrwl*llqllnwsllvn
lnlisivllninfqmlpnpypmqpif*wlqpklsllksplkrisisinslslviklkkwn
nkwqfsd*kkn*kslekv*vllernnr

Frame B:
*qes*trvn*s*qrftncksfngfn*ccl*na*fnrisntl**l*nfcfhsisfrilcyl
yfsctffnkfn**g***rsygffn*kilwsisnieklff*mqvqlqvylvllqyqiyhqi
lqslleklerhqrpnhrqfhhql*rnhqhhhlnkfqfqyqhlysfvkryqwfmahlmvcn
nnnkiivcgvslvpin---

---wyq*fgcigsts*r*kn*sreeifrewf*fg*cc*t*tigcr*nh*rcckliigrki
kesskegg*ftrcc*gnp*gcygnhwcyfhigwcrhfstkrtcrkg*nk*wwstl*kgsn
lg*rfnlcckiccpsh*sfgr*cqqdsiwsrdprwyrrsshcnfdgcnccnfsiglcl*i
*i*yq*sfst*tfkccqiriqcnqsfsgcsqscrc*srl*rgyrfr*thchwl*s*rngt
tngnsqirkrirsr*krfkcy*keti

Frame C:
sknhrpesievdkglqtvnrlmdltdavfrmhnsivsatpyndcktsafiplvlesyaiy
ilvvhfltnlidkvdnkevmdfsikrfygqyqtlrnfffkckcnykci*cycstkfttrs
skvy*ks*rgtkgqttgnsttsckgttnttt*tnsssstntcihssngtsglwrtlwyat
tttr**yvgsvwfqst---

---GINNLDALAQQADAERIEAEKKYLENGFDLDDVAERELLAAAKTIEDAANSLLAAKS
KRAPKKEGDSPDVAEAILEAAMAITGATSTLVGAATLAQRERVEKGRTSDGGPLYRKDPT
WAEGLISAAKSVAQATKALVDNANKTASGQEIPGGTEEALIATSMAVTAATSQLVSACKS
KSDINSPSQHKLSNAAKSVSNATNLLVAAAKAVAAKVASEEDIDFDKLTVTGYKVKEMEQ
QMAILRLEKELEVARKGLSVIRKKQ*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR147 (CHR147Q) /CSM/CH/CHR1-B/CHR147Q.Seq.d/                       2648   0.0
SHA557 (SHA557Q) /CSM/SH/SHA5-C/SHA557Q.Seq.d/                       1570   0.0
SHA409 (SHA409Q) /CSM/SH/SHA4-A/SHA409Q.Seq.d/                       1570   0.0
CHS178 (CHS178Q) /CSM/CH/CHS1-D/CHS178Q.Seq.d/                       1570   0.0
SHK510 (SHK510Q) /CSM/SH/SHK5-A/SHK510Q.Seq.d/                       1566   0.0
CHI561 (CHI561Q) /CSM/CH/CHI5-C/CHI561Q.Seq.d/                       1566   0.0
CHN144 (CHN144Q) /CSM/CH/CHN1-B/CHN144Q.Seq.d/                       1564   0.0
CHR474 (CHR474Q) /CSM/CH/CHR4-D/CHR474Q.Seq.d/                       1562   0.0
SHL395 (SHL395Q) /CSM/SH/SHL3-D/SHL395Q.Seq.d/                       1560   0.0
VHQ296 (VHQ296Q) /CSM/VH/VHQ2-D/VHQ296Q.Seq.d/                       1556   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U14576|U14576.2 Dictyostelium discoideum filopodin (talA) gene, complete cds.    56   0.002  2
AC144481|AC144481.4 Medicago truncatula clone mth2-35e10, complete sequence.    38   0.28   2
AC008051|AC008051.3 Sequence of BAC F19C14 from Arabidopsis thaliana chromosome 1, complete sequence.    34   0.29   2
AI440613|AI440613.1 sa68d09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-4458 5', mRNA sequence.    40   1.2    2
AC117854|AC117854.4 Rattus norvegicus clone CH230-158L11, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    46   2.0    1
AC127056|AC127056.5 Rattus norvegicus clone CH230-21O22, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    46   2.0    1
AX079353|AX079353.1 Sequence 97 from Patent WO0107611.             46   2.0    1
CZ509360|CZ509360.1 GMW2-38N17a.g1 GMW2 Glycine max genomic, genomic survey sequence.    46   2.0    1
CP000040|CP000040.1 Bacillus cereus E33L plasmid pE33L466, complete sequence.    46   2.0    1
AL845327|AL845327.5 Mouse DNA sequence from clone RP23-213J9 on chromosome X.    46   2.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K81) RecName: Full=Talin-B;                                       173   1e-41
AB023655_1(AB023655|pid:none) Dictyostelium discoideum talB mRNA...   173   1e-41
(P54633) RecName: Full=Talin-A; AltName: Full=Filopodin;              153   2e-35
A57036(A57036)talin - slime mold (Dictyostelium discoideum)           153   2e-35
AE017345_306(AE017345|pid:none) Cryptococcus neoformans var. neo...   145   4e-33
EF107688_1(EF107688|pid:none) Emericella nidulans SlaB (slaB) ge...   140   8e-32
EF156414_1(EF156414|pid:none) Ascosphaera apis cytoskeleton asse...   139   3e-31
CP000501_90(CP000501|pid:none) Pichia stipitis CBS 6054 chromoso...   138   4e-31
EF363034_3(EF363034|pid:none) Neosartorya fischeri apn1 (APN1) a...   135   3e-30
AM920435_781(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   135   3e-30

 psg: 0.75  gvh: 0.57  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for CHR147 is cyt

>CHR147F.Seq
ATAGCAAGAATCATAGACCCGAGTCAATTGAAGTTGACAAAGGTTTACAAACTGTAAATC
GTTTAATGGATTTAACTGATGCTGTCTTTAGAATGCATAATTCAATCGTATCAGCAACAC
CTTATAATGATTGTAAAACTTCTGCTTTCATTCCATTAGTTTTAGAATCTTATGCTATCT
ATATTTTAGTTGTACATTTTTTAACAAATTTAATTGATAAGGTTGATAATAAAGAAGTTA
TGGATTTTTCAATTAAAAGATTTTATGGTCAATATCAAACATTGAGAAACTTTTTTTTTA
AATGCAAGTGCAATTACAAGTGTATCTAGTGTTATTGCAGTACCAAATTTACCACCAGAT
CCTCCAAAGTTTATTAGAAAAGTTAGAGAGGCACCAAAGGCCAAACCACAGGCAATTCCA
CCACCAGTTGTAAAGGAACCACCAACACCACCACCTGAACAAATTCCAGTTCCAGTACCA
ACACCTGTATTCATTCGTCAAACGGTACCAGTGGTTTATGGCGCACCTTATGGTATGCAA
CAACAACAACAAGATAATAGTATGTGGGGTCAGTTTGGTTCCAATCAACCANNNNNNNNN
N

>CHR147Z.Seq
NNNNNNNNNNCTGGTATCAATAATTTGGATGCATTGGCTCAACAAGCTGACGCTGAAAGA
ATTGAAGCCGAGAAGAAATATTTAGAGAATGGTTTTGATTTGGATGATGTTGCTGAACGT
GAACTATTGGCTGCCGCTAAAACCATTGAAGATGCTGCAAACTCATTATTGGCCGCAAAA
TCAAAGAGAGCTCCAAAGAAGGAGGGTGATTCACCAGATGTTGCTGAGGCAATCCTTGAG
GCTGCTATGGCAATCACTGGTGCTACTTCCACATTGGTTGGTGCCGCCACTTTAGCACAA
AGAGAACGTGTCGAAAAGGGTAGAACAAGTGATGGTGGTCCACTCTATAGAAAGGATCCA
ACTTGGGCTGAAGGTTTAATCTCTGCTGCAAAATCTGTTGCCCAAGCCACTAAAGCTTTG
GTAGATAATGCCAACAAGACAGCATCTGGTCAAGAGATCCCAGGTGGTACAGAAGAAGCT
CTCATTGCAACTTCGATGGCTGTAACTGCTGCAACTTCTCAATTGGTCTCTGCTTGTAAA
TCTAAATCTGATATCAATAGTCCTTCTCAACATAAACTTTCAAATGCTGCCAAATCCGTA
TCCAATGCAACCAATCTTTTAGTGGCTGCAGCCAAAGCTGTCGCTGCTAAAGTCGCCTCT
GAAGAGGATATCGATTTCGATAAACTCACTGTCACTGGTTATAAAGTTAAAGAAATGGAA
CAACAAATGGCAATTCTCAGATTAGAAAAAGAATTAGAAGTCGCTAGAAAAGGTTTAAGT
GTTATTAGAAAGAAACAATAGA

>CHR147P.Seq
ATAGCAAGAATCATAGACCCGAGTCAATTGAAGTTGACAAAGGTTTACAAACTGTAAATC
GTTTAATGGATTTAACTGATGCTGTCTTTAGAATGCATAATTCAATCGTATCAGCAACAC
CTTATAATGATTGTAAAACTTCTGCTTTCATTCCATTAGTTTTAGAATCTTATGCTATCT
ATATTTTAGTTGTACATTTTTTAACAAATTTAATTGATAAGGTTGATAATAAAGAAGTTA
TGGATTTTTCAATTAAAAGATTTTATGGTCAATATCAAACATTGAGAAACTTTTTTTTTA
AATGCAAGTGCAATTACAAGTGTATCTAGTGTTATTGCAGTACCAAATTTACCACCAGAT
CCTCCAAAGTTTATTAGAAAAGTTAGAGAGGCACCAAAGGCCAAACCACAGGCAATTCCA
CCACCAGTTGTAAAGGAACCACCAACACCACCACCTGAACAAATTCCAGTTCCAGTACCA
ACACCTGTATTCATTCGTCAAACGGTACCAGTGGTTTATGGCGCACCTTATGGTATGCAA
CAACAACAACAAGATAATAGTATGTGGGGTCAGTTTGGTTCCAATCAACCA---------
-CTGGTATCAATAATTTGGATGCATTGGCTCAACAAGCTGACGCTGAAAGAATTGAAGCC
GAGAAGAAATATTTAGAGAATGGTTTTGATTTGGATGATGTTGCTGAACGTGAACTATTG
GCTGCCGCTAAAACCATTGAAGATGCTGCAAACTCATTATTGGCCGCAAAATCAAAGAGA
GCTCCAAAGAAGGAGGGTGATTCACCAGATGTTGCTGAGGCAATCCTTGAGGCTGCTATG
GCAATCACTGGTGCTACTTCCACATTGGTTGGTGCCGCCACTTTAGCACAAAGAGAACGT
GTCGAAAAGGGTAGAACAAGTGATGGTGGTCCACTCTATAGAAAGGATCCAACTTGGGCT
GAAGGTTTAATCTCTGCTGCAAAATCTGTTGCCCAAGCCACTAAAGCTTTGGTAGATAAT
GCCAACAAGACAGCATCTGGTCAAGAGATCCCAGGTGGTACAGAAGAAGCTCTCATTGCA
ACTTCGATGGCTGTAACTGCTGCAACTTCTCAATTGGTCTCTGCTTGTAAATCTAAATCT
GATATCAATAGTCCTTCTCAACATAAACTTTCAAATGCTGCCAAATCCGTATCCAATGCA
ACCAATCTTTTAGTGGCTGCAGCCAAAGCTGTCGCTGCTAAAGTCGCCTCTGAAGAGGAT
ATCGATTTCGATAAACTCACTGTCACTGGTTATAAAGTTAAAGAAATGGAACAACAAATG
GCAATTCTCAGATTAGAAAAAGAATTAGAAGTCGCTAGAAAAGGTTTAAGTGTTATTAGA
AAGAAACAATAGA