>CHR110 (CHR110Q) /CSM/CH/CHR1-A/CHR110Q.Seq.d/
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGGAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TCATTTATTGCTCCACCTGGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGC
AAGGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCGXXXXXXXXXXGATAGTATT
ACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTAT
TTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAAT
GATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCA
GCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCT
GCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGC
TCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTC
ATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCA
TTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTC
AATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACC
ACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAA
TATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTNCTT
TTTTAATAAAAAAAAAAAAAATAA

*f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgis
ss*l**lwstrkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnercheki
rw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wi
iycstwevvlyik*i*SILQGEVMNGTGNLLYP---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*xlf**kkkk*

Frame A:
infnlyklpyylkmttnnpfdkvkdvlksqdqtynfynlsklqdprieklpysirilles
avrncdnfgvhekdvenilnwentankveipfkparvllqdftgvpavvdlaamrdamkr
lggdpakinplvpvdlvidhsvqvdvsrtvdaleqnqkiefhrnherfsflkwgaqafdg
sfiappgkwyctsskfrvyckekl*melvtsyi---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*xlf**kkkk*

Frame B:
liliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnq
qfvivitleytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd
*vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmd
hlllhlgsgivhqvnleyiarrsyewnw*plis---

---ivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvl
linwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtf
kelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklv
kp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinxffnkkkkn

Frame C:
*f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgis
ss*l**lwstrkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnercheki
rw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wi
iycstwevvlyik*i*SILQGEVMNGTGNLLYP---

---*yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc
**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsips
rn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nws
nrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlixflikkkki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHR110 (CHR110Q) /CSM/CH/CHR1-A/CHR110Q.Seq.d/                       2565   0.0
VHE337 (VHE337Q) /CSM/VH/VHE3-B/VHE337Q.Seq.d/                       1334   0.0
VHA182 (VHA182Q) /CSM/VH/VHA1-D/VHA182Q.Seq.d/                       1334   0.0
VHA161 (VHA161Q) /CSM/VH/VHA1-C/VHA161Q.Seq.d/                       1334   0.0
SHG221 (SHG221Q) /CSM/SH/SHG2-A/SHG221Q.Seq.d/                       1334   0.0
SHA509 (SHA509Q) /CSM/SH/SHA5-A/SHA509Q.Seq.d/                       1334   0.0
CHQ778 (CHQ778Q) /CSM/CH/CHQ7-D/CHQ778Q.Seq.d/                       1334   0.0
CHM233 (CHM233Q) /CSM/CH/CHM2-B/CHM233Q.Seq.d/                       1334   0.0
CHL182 (CHL182Q) /CSM/CH/CHL1-D/CHL182Q.Seq.d/                       1334   0.0
AHO185 (AHO185Q) /CSM/AH/AHO1-D/AHO185Q.Seq.d/                       1334   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    56   2e-30  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-25  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   7e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   2e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   2e-18  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   3e-18  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   5e-18  4
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   5e-17  4
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   5e-17  6
D29629|D29629.1 Cucurbita cv. Kurokawa Amakuri mRNA for aconitase, complete cds.    54   1e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   443   e-123
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   291   3e-77
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   290   8e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   288   3e-76
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   287   5e-76
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   287   5e-76
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   284   4e-75
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   283   7e-75
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   283   9e-75
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   282   2e-74

 psg: 0.75  gvh: 0.33  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHR110 is nuc

>CHR110F.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGGAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TCATTTATTGCTCCACCTGGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGC
AAGGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCGNNNNNNNNNN

>CHR110Z.Seq
NNNNNNNNNNGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAA
ATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTA
TGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTAT
TGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTT
AATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTT
GGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCT
TCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGT
TGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCT
CACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGG
TCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGA
TACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAA
ACTTTTACATTAATTNCTTTTTTAATAAAAAAAAAAAAAATAA

>CHR110P.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGGAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TCATTTATTGCTCCACCTGGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGC
AAGGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCG----------GATAGTATT
ACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTAT
TTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAAT
GATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCA
GCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCT
GCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGC
TCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTC
ATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCA
TTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTC
AATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACC
ACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAA
TATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTNCTT
TTTTAATAAAAAAAAAAAAAATAA