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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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AFH387
Library AF
(Link to library)
Clone ID AFH387
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14923-1
Original site URL
Representative seq. ID AFH387P
(Link to Original site)
Representative DNA sequence 
>AFH387 (AFH387Q) /CSM/AF/AFH3-D/AFH387Q.Seq.d/
ATAAAAAAAATTTTCTAAAAAAACACACACACCTACTTTGATATATATTATATTTAATAT
TCAGAGTAAAAAATAAAAGAATTTTTATTTGTGTTTTATTAAAAAAAAAAAGAAATAAAA
TAAATAAAAAAAAAAXXXXXXXXXXACATCCAGAGTTATTTGAAAGTTTAGGTATCGCTC
AACCAAAGGGTGTACTCTTGTATGGTCCACCAGGTACTGGTAAAACTTTGTTAGCTCGTG
CCGTTGCTCATCATACCGATTGCACATTCATTAGAGTTAGTGGTTCCGAATTGGTTCAAA
AATATATTGGTGAAGGTAGTCGTATGGTTCGTGAATTATTCATTATGGCTAGAGAACATG
CTCCTTCAATCATTTTCATGGATGAAATCGATTCCATTGGTTCATCTCGTGGTGAATCTG
GTTCCGGTGGTGGTGATAGTGAAGTTCAACGTACTATGTTAGAGTTACTCAATCAATTGG
ATGGTTTTGAAAGTACTAAAAACATTAAAGTACTCATGTGTACAAATCGTATTGATATTT
TAGATCCAGCTTTATTAAGACCAGGTAGAATTGATAGAAAAATTGAATTTCCAAATCCAG
GTGATGCTGGTCGTTTAGATATTTTAAAGATCCANTCAACNANAATGNAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
kknflkkhthll*YILYLIFRVKNKRIFICVLLKKKRNKINKKK---

---HPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEG
SRMVRELFIMAREHAPSIIFMDEIDSIGSSRGESGSGGGDSEVQRTMLELLNQLDGFEST
KNIKVLMCTNRIDILDPALLRPGRIDRKIEFPNPGDAGRLDILKIXSTXMX
Translated Amino Acid sequence (All Frames) 
Frame A:
ikkif*knthtyfdiyyi*yse*kikeflfvfy*kkkeik*ikkk---

---tsrvi*kfryrstkgctlvwstryw*nfvsscrcssyrlhih*s*wfrigskiyw*r
*sygs*iihyg*rtcsfnhfhg*nrfhwfisw*iwfrww***sstyyvrvtqsigwf*ky
*kh*sthvyksy*yfrssfiktr*n**kn*isksr*cwsfryfkdpxnxnx

Frame B:
*kkfskkthtptliyiifniqskk*knfylcfikkkkk*nk*kk---

---HPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEG
SRMVRELFIMAREHAPSIIFMDEIDSIGSSRGESGSGGGDSEVQRTMLELLNQLDGFEST
KNIKVLMCTNRIDILDPALLRPGRIDRKIEFPNPGDAGRLDILKIXSTXMX

Frame C:
kknflkkhthll*YILYLIFRVKNKRIFICVLLKKKRNKINKKK---

---iqsylkv*vslnqrvyscmvhqvlvklc*lvplliipiahslelvvpnwfknilvkv
vvwfvnyslwlenmllqsfswmksiplvhlvvnlvpvvvivkfnvlc*sysinwmvlkvl
ktlkyscvqivlif*iqly*dqvelieklnfqiqvmlvv*if*rsxqxxx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH387 (AFH387Q) /CSM/AF/AFH3-D/AFH387Q.Seq.d/                        977   0.0
VFI701 (VFI701Q) /CSM/VF/VFI7-A/VFI701Q.Seq.d/                        973   0.0
AFD596 (AFD596Q) /CSM/AF/AFD5-D/AFD596Q.Seq.d/                        973   0.0
VFH603 (VFH603Q) /CSM/VF/VFH6-A/VFH603Q.Seq.d/                        969   0.0
CHB582 (CHB582Q) /CSM/CH/CHB5-D/CHB582Q.Seq.d/                        969   0.0
AFJ867 (AFJ867Q) /CSM/AF/AFJ8-C/AFJ867Q.Seq.d/                        969   0.0
AFF418 (AFF418Q) /CSM/AF/AFF4-A/AFF418Q.Seq.d/                        969   0.0
AFC851 (AFC851Q) /CSM/AF/AFC8-C/AFC851Q.Seq.d/                        969   0.0
AFB296 (AFB296Q) /CSM/AF/AFB2-D/AFB296Q.Seq.d/                        969   0.0
SSF362 (SSF362Q) /CSM/SS/SSF3-C/SSF362Q.Seq.d/                        965   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L16579|L16579.1 Dictyostelium discoideum HIV1 TAT-binding protein homologue (TBP1O) mRNA, 3' end.   973   0.0    1
AL407935|AL407935.1 T7 end of clone AV0AA005E11 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.   137   6e-61  3
AX489025|AX489025.1 Sequence 6325 from Patent WO02053728.          98   3e-60  5
AZ926397|AZ926397.1 476.dis06d12.s1 Saccharomyces castellii NRRL Y-12630 Saccharomyces castellii genomic clone 476.dis06d12.s1, DNA sequence.    96   2e-50  3
BG101557|BG101557.2 QW25 Apis mellifera larval caste mRNA Apis mellifera cDNA clone QW25 3' similar to Drosophila Pros45, mRNA sequence.    82   1e-42  4
U41812|U41812.1 Naegleria fowleri Tat-binding protein homolog mRNA, complete cds.    66   5e-42  5
AZ539996|AZ539996.1 ENTGG47TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    82   5e-38  3
BH161295|BH161295.1 ENTQX52TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    82   5e-38  3
BH147344|BH147344.1 ENTPF31TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    82   5e-38  3
AL403948|AL403948.1 T3 end of clone AT0AA012A06 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    78   4e-37  4
dna update 2004. 5.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34124) RecName: Full=26S protease regulatory subunit 8; AltNam...   280   2e-74
(Q25544) RecName: Full=26S protease regulatory subunit 8 homolog...   263   4e-69
FB781822_1(FB781822|pid:none) Sequence 1095 from Patent WO200803...   258   1e-67
AY011124_1(AY011124|pid:none) Dactylis glomerata 26S proteasome ...   257   2e-67
AK226206_1(AK226206|pid:none) Arabidopsis thaliana mRNA for 26S ...   256   3e-67
AB035272_1(AB035272|pid:none) Matricaria chamomilla McTBP1 mRNA ...   256   3e-67
EU162611_6(EU162611|pid:none) Capsella rubella clone contig46 BA...   256   3e-67
AF123395_1(AF123395|pid:none) Arabidopsis thaliana 26S proteasom...   256   3e-67
EF678275_1(EF678275|pid:none) Picea sitchensis clone WS02911_P12...   256   3e-67
AB033537_1(AB033537|pid:none) Oryza sativa Japonica Group OsRPT6...   256   4e-67
protein update 2009. 4.21
PSORT 

 psg: 0.64  gvh: 0.37  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.05
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 24.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFH387 is mit
5' end seq. ID AFH387F
5' end seq. 
>AFH387F.Seq
ATAAAAAAAATTTTCTAAAAAAACACACACACCTACTTTGATATATATTATATTTAATAT
TCAGAGTAAAAAATAAAAGAATTTTTATTTGTGTTTTATTAAAAAAAAAAAGAAATAAAA
TAAATAAAAAAAAAA----------
Length of 5' end seq. 135
3' end seq. ID AFH387Z
3' end seq. 
>AFH387Z.Seq
----------ACATCCAGAGTTATTTGAAAGTTTAGGTATCGCTCAACCAAAGGGTGTAC
TCTTGTATGGTCCACCAGGTACTGGTAAAACTTTGTTAGCTCGTGCCGTTGCTCATCATA
CCGATTGCACATTCATTAGAGTTAGTGGTTCCGAATTGGTTCAAAAATATATTGGTGAAG
GTAGTCGTATGGTTCGTGAATTATTCATTATGGCTAGAGAACATGCTCCTTCAATCATTT
TCATGGATGAAATCGATTCCATTGGTTCATCTCGTGGTGAATCTGGTTCCGGTGGTGGTG
ATAGTGAAGTTCAACGTACTATGTTAGAGTTACTCAATCAATTGGATGGTTTTGAAAGTA
CTAAAAACATTAAAGTACTCATGTGTACAAATCGTATTGATATTTTAGATCCAGCTTTAT
TAAGACCAGGTAGAATTGATAGAAAAATTGAATTTCCAAATCCAGGTGATGCTGGTCGTT
TAGATATTTTAAAGATCCANTCAACNANAATGNAT
Length of 3' end seq. 505
Connected seq. ID AFH387P
Connected seq. 
>AFH387P.Seq
ATAAAAAAAATTTTCTAAAAAAACACACACACCTACTTTGATATATATTATATTTAATAT
TCAGAGTAAAAAATAAAAGAATTTTTATTTGTGTTTTATTAAAAAAAAAAAGAAATAAAA
TAAATAAAAAAAAAA----------ACATCCAGAGTTATTTGAAAGTTTAGGTATCGCTC
AACCAAAGGGTGTACTCTTGTATGGTCCACCAGGTACTGGTAAAACTTTGTTAGCTCGTG
CCGTTGCTCATCATACCGATTGCACATTCATTAGAGTTAGTGGTTCCGAATTGGTTCAAA
AATATATTGGTGAAGGTAGTCGTATGGTTCGTGAATTATTCATTATGGCTAGAGAACATG
CTCCTTCAATCATTTTCATGGATGAAATCGATTCCATTGGTTCATCTCGTGGTGAATCTG
GTTCCGGTGGTGGTGATAGTGAAGTTCAACGTACTATGTTAGAGTTACTCAATCAATTGG
ATGGTTTTGAAAGTACTAAAAACATTAAAGTACTCATGTGTACAAATCGTATTGATATTT
TAGATCCAGCTTTATTAAGACCAGGTAGAATTGATAGAAAAATTGAATTTCCAAATCCAG
GTGATGCTGGTCGTTTAGATATTTTAAAGATCCANTCAACNANAATGNAT
Length of connected seq. 640
Full length Seq ID -
Full length Seq. -
Length of full length seq. -