>CHQ457 (CHQ457Q) /CSM/CH/CHQ4-C/CHQ457Q.Seq.d/
AAATTTTTTAAAAATGATGGCAAAACCAAATGGTAAAATTCAATTCAAGAATTTACAAAG
TCATGGAGTATTAGCGGATCCAGATTTTCCAAAGAGAACGTGGAAATTATTAAAGACAGC
AATGAGACAAATTCATCAACAAAATGCAAGTAATCTCTCATTTGAAGAGTTGTATAGAAA
CGGATACAATATGGTATTACAAAAACATGGTGATTTATTATACAATAATCTAAAGAAGAT
GGTCGATAAACATTTGAAAGCGGTTGCTAAAACTGTATCAGAATCAATCGATGAAAAGTT
TCTACTAGAATTAAACTCGAGTTGGATCAATCACAAGACTTCAATGTTAATGATTAGAGA
TATCTTGATGTATATGGATAGAAACTATGTTAAACAAAATAATCTCTCTAGTGTTTTTGA
TCTTGGACTTTACCTTTTCAGAGATAATGTGGCTCATTGTTXXXXXXXXXXACTAATATG
GGTACCGCAGAAATTAAAGCAAACTTCCTTTCGAAATCTCACGAACTTCAAGGTTCNTCC
TATCAAATGGTCATCTTATTACTCTTTAATGACCAATCGAAATTAACCTTTAAAGAGATT
GCTGATCAAACTGGTATTCCAACTATTGATTTAAAGAGAAATCTTTTAGCTTTAACAAAT
CCAAAGAATAAAATTTTAGATAGAGAATTACCATCAACAACATCATCAACAACAACAACA
ACAACAACAGCAACATCATCATCAACTTCAACTTCACCATCTAGTAGTTCATCTTCAATC
TCCACTCCAACACCAAGTAAATCAATTGATGAATCCGATGTATTCGCTTTTAATACTAAA
TTTAAGAGTAAATTATTCCGTGTTAAAGTTATGGCAGTCGTTCAAAAAGAAACACCAGTT
GAAGAGAAAGAAACTCGTGATAAAGTTGATGAAGATAGAAAACATCAAATTGAAGCTTCC
ATCGTTCGTATTATGAAAGCTCGTAAAACACTTGAACACTCTAATCTTGTCTCTGAAGTC
ATCAAGCAACTTCAATCTCGTTTTGTTCCAAATCCAGTCATTGTAAAGAAACGTATCGAA
TCTTTAATGAAAGAGA

NFLKMMAKPNGKIQFKNLQSHGVLADPDFPKRTWKLLKTAMRQIHQQNASNLSFEELYRN
GYNMVLQKHGDLLYNNLKKMVDKHLKAVAKTVSESIDEKFLLELNSSWINHKTSMLMIRD
ILMYMDRNYVKQNNLSSVFDLGLYLFRDNVAHC---

---TNMGTAEIKANFLSKSHELQGSSYQMVILLLFNDQSKLTFKEIADQTGIPTIDLKRN
LLALTNPKNKILDRELPSTTSSTTTTTTTATSSSTSTSPSSSSSSISTPTPSKSIDESDV
FAFNTKFKSKLFRVKVMAVVQKETPVEEKETRDKVDEDRKHQIEASIVRIMKARKTLEHS
NLVSEVIKQLQSRFVPNPVIVKKRIESLMKE

Frame A:
kffkndgktkw*nsiqeftkswsisgsrfskenveiikdsnetnsstkck*sli*rvv*k
riqygitktw*fiiq*skedgr*tfesgc*ncirinr*kvstrikleldqsqdfnvnd*r
yldvyg*klc*tk*sl*cf*swtlpfqr*cgsl---

---TNMGTAEIKANFLSKSHELQGSSYQMVILLLFNDQSKLTFKEIADQTGIPTIDLKRN
LLALTNPKNKILDRELPSTTSSTTTTTTTATSSSTSTSPSSSSSSISTPTPSKSIDESDV
FAFNTKFKSKLFRVKVMAVVQKETPVEEKETRDKVDEDRKHQIEASIVRIMKARKTLEHS
NLVSEVIKQLQSRFVPNPVIVKKRIESLMKE

Frame B:
NFLKMMAKPNGKIQFKNLQSHGVLADPDFPKRTWKLLKTAMRQIHQQNASNLSFEELYRN
GYNMVLQKHGDLLYNNLKKMVDKHLKAVAKTVSESIDEKFLLELNSSWINHKTSMLMIRD
ILMYMDRNYVKQNNLSSVFDLGLYLFRDNVAHC---

---liwvpqklkqtsfrnltnfkvxpikwssyyslmtnrn*plkrlliklvfqlli*rei
f*l*qiqrikf*ienyhqqhhqqqqqqqqqhhhqlqlhhlvvhlqsplqhqvnqlmnpmy
sllilnlrvnysvlklwqsfkkkhqlkrkklviklmkieniklklpsfvl*klvkhlntl
ilslksssnfnlvlfqiqsl*rnvsnl**kr

Frame C:
if*k*wqnqmvkfnsriykvmey*riqifqrergny*rqq*dkfinkmqvishlksciet
dtiwyyknmviyytii*rrwsini*krllklyqnqsmksfy*n*trvgsitrlqc**lei
s*ciwietmlnkiislvflildftfseimwliv---

---*ygyrrn*sklpfeisrtsrfxlsnghlitl**pieinl*rdc*snwysny*fkeks
fsfnkske*nfr*ritinniinnnnnnnsniiinfnfti**fifnlhsntk*in**irci
rf*y*i*e*iipc*sygsrskrnts*rerns**s**r*ktsn*sfhrsyyess*nt*tl*
scl*shqatsisfcsksshcketyrifner

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHQ457 (CHQ457Q) /CSM/CH/CHQ4-C/CHQ457Q.Seq.d/                       1310   0.0
VHQ327 (VHQ327Q) /CSM/VH/VHQ3-B/VHQ327Q.Seq.d/                        716   0.0
VHB307 (VHB307Q) /CSM/VH/VHB3-A/VHB307Q.Seq.d/                        702   0.0
SSI609 (SSI609Q) /CSM/SS/SSI6-A/SSI609Q.Seq.d/                        702   0.0
AFG826 (AFG826Q) /CSM/AF/AFG8-B/AFG826Q.Seq.d/                        702   0.0
VHH207 (VHH207Q) /CSM/VH/VHH2-A/VHH207Q.Seq.d/                        700   0.0
SSC509 (SSC509Q) /CSM/SS/SSC5-A/SSC509Q.Seq.d/                        700   0.0
CHC421 (CHC421Q) /CSM/CH/CHC4-A/CHC421Q.Seq.d/                        700   0.0
CFK287 (CFK287Q) /CSM/CF/CFK2-D/CFK287Q.Seq.d/                        700   0.0
VSK353 (VSK353Q) /CSM/VS/VSK3-C/VSK353Q.Seq.d/                        630   e-179

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF144717|AF144717.1 Dictyostelium discoideum CulB mRNA, complete cds.    50   5e-07  2
AW398090|AW398090.1 EST297973 L. pennellii trichome, Cornell University Lycopersicon pennellii cDNA clone cLPT1D6 5', mRNA sequence.    54   0.006  1
DT270276|DT270276.1 JGI_CAAV3409.fwd CAAV Pimephales promelas testis 7-8 month adults, males and females pooled (H) Pimephales promelas cDNA clone CAAV3409 5', mRNA sequence.    54   0.006  1
DT298153|DT298153.1 JGI_CAAW9022.fwd CAAW Pimephales promelas testis 7-8 month adults, males and females pooled (M) Pimephales promelas cDNA clone CAAW9022 5', mRNA sequence.    54   0.006  1
DT342033|DT342033.1 JGI_CAAX7432.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX7432 5', mRNA sequence.    54   0.006  1
DT145646|DT145646.1 JGI_ANNO26747.fwd ANNO Pimephales promelas Whole (M) Pimephales promelas cDNA clone ANNO26747 5', mRNA sequence.    54   0.006  1
DT199084|DT199084.1 JGI_CAAS6447.fwd CAAS Pimephales promelas brain 7-8 month adults, males and females pooled (H) Pimephales promelas cDNA clone CAAS6447 5', mRNA sequence.    54   0.006  1
DT136086|DT136086.1 JGI_ANNO16238.fwd ANNO Pimephales promelas Whole (M) Pimephales promelas cDNA clone ANNO16238 5', mRNA sequence.    54   0.006  1
DT155403|DT155403.1 JGI_ANNO32950.fwd ANNO Pimephales promelas Whole (M) Pimephales promelas cDNA clone ANNO32950 5', mRNA sequence.    54   0.006  1
DT302783|DT302783.1 JGI_CAAW11666.fwd CAAW Pimephales promelas testis 7-8 month adults, males and females pooled (M) Pimephales promelas cDNA clone CAAW11666 5', mRNA sequence.    54   0.006  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NZ5) RecName: Full=Cullin-3;          Short=CUL-3; AltName: ...   306   6e-82
AL606454_2(AL606454|pid:none) Oryza sativa genomic DNA, chromoso...   167   7e-40
AK072402_1(AK072402|pid:none) Oryza sativa Japonica Group cDNA c...   166   1e-39
FB802359_1(FB802359|pid:none) Sequence 21632 from Patent WO20080...   165   3e-39
AM484063_2(AM484063|pid:none) Vitis vinifera contig VV78X066416....   164   6e-39
BT069454_1(BT069454|pid:none) Zea mays full-length cDNA clone ZM...   160   7e-38
FB802477_1(FB802477|pid:none) Sequence 21750 from Patent WO20080...   160   7e-38
AK230392_1(AK230392|pid:none) Arabidopsis thaliana mRNA for cull...   158   3e-37
A86395(A86395) hypothetical protein T2P11.2 [imported] - Arabido...   158   3e-37
AJ319540_1(AJ319540|pid:none) Arabidopsis thaliana partial mRNA ...   158   3e-37

 psg: 0.56  gvh: 0.49  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for CHQ457 is cyt

>CHQ457F.Seq
AAATTTTTTAAAAATGATGGCAAAACCAAATGGTAAAATTCAATTCAAGAATTTACAAAG
TCATGGAGTATTAGCGGATCCAGATTTTCCAAAGAGAACGTGGAAATTATTAAAGACAGC
AATGAGACAAATTCATCAACAAAATGCAAGTAATCTCTCATTTGAAGAGTTGTATAGAAA
CGGATACAATATGGTATTACAAAAACATGGTGATTTATTATACAATAATCTAAAGAAGAT
GGTCGATAAACATTTGAAAGCGGTTGCTAAAACTGTATCAGAATCAATCGATGAAAAGTT
TCTACTAGAATTAAACTCGAGTTGGATCAATCACAAGACTTCAATGTTAATGATTAGAGA
TATCTTGATGTATATGGATAGAAACTATGTTAAACAAAATAATCTCTCTAGTGTTTTTGA
TCTTGGACTTTACCTTTTCAGAGATAATGTGGCTCATTGTTNNNNNNNNNN

>CHQ457Z.Seq
NNNNNNNNNNACTAATATGGGTACCGCAGAAATTAAAGCAAACTTCCTTTCGAAATCTCA
CGAACTTCAAGGTTCNTCCTATCAAATGGTCATCTTATTACTCTTTAATGACCAATCGAA
ATTAACCTTTAAAGAGATTGCTGATCAAACTGGTATTCCAACTATTGATTTAAAGAGAAA
TCTTTTAGCTTTAACAAATCCAAAGAATAAAATTTTAGATAGAGAATTACCATCAACAAC
ATCATCAACAACAACAACAACAACAACAGCAACATCATCATCAACTTCAACTTCACCATC
TAGTAGTTCATCTTCAATCTCCACTCCAACACCAAGTAAATCAATTGATGAATCCGATGT
ATTCGCTTTTAATACTAAATTTAAGAGTAAATTATTCCGTGTTAAAGTTATGGCAGTCGT
TCAAAAAGAAACACCAGTTGAAGAGAAAGAAACTCGTGATAAAGTTGATGAAGATAGAAA
ACATCAAATTGAAGCTTCCATCGTTCGTATTATGAAAGCTCGTAAAACACTTGAACACTC
TAATCTTGTCTCTGAAGTCATCAAGCAACTTCAATCTCGTTTTGTTCCAAATCCAGTCAT
TGTAAAGAAACGTATCGAATCTTTAATGAAAGAGA

>CHQ457P.Seq
AAATTTTTTAAAAATGATGGCAAAACCAAATGGTAAAATTCAATTCAAGAATTTACAAAG
TCATGGAGTATTAGCGGATCCAGATTTTCCAAAGAGAACGTGGAAATTATTAAAGACAGC
AATGAGACAAATTCATCAACAAAATGCAAGTAATCTCTCATTTGAAGAGTTGTATAGAAA
CGGATACAATATGGTATTACAAAAACATGGTGATTTATTATACAATAATCTAAAGAAGAT
GGTCGATAAACATTTGAAAGCGGTTGCTAAAACTGTATCAGAATCAATCGATGAAAAGTT
TCTACTAGAATTAAACTCGAGTTGGATCAATCACAAGACTTCAATGTTAATGATTAGAGA
TATCTTGATGTATATGGATAGAAACTATGTTAAACAAAATAATCTCTCTAGTGTTTTTGA
TCTTGGACTTTACCTTTTCAGAGATAATGTGGCTCATTGTT----------ACTAATATG
GGTACCGCAGAAATTAAAGCAAACTTCCTTTCGAAATCTCACGAACTTCAAGGTTCNTCC
TATCAAATGGTCATCTTATTACTCTTTAATGACCAATCGAAATTAACCTTTAAAGAGATT
GCTGATCAAACTGGTATTCCAACTATTGATTTAAAGAGAAATCTTTTAGCTTTAACAAAT
CCAAAGAATAAAATTTTAGATAGAGAATTACCATCAACAACATCATCAACAACAACAACA
ACAACAACAGCAACATCATCATCAACTTCAACTTCACCATCTAGTAGTTCATCTTCAATC
TCCACTCCAACACCAAGTAAATCAATTGATGAATCCGATGTATTCGCTTTTAATACTAAA
TTTAAGAGTAAATTATTCCGTGTTAAAGTTATGGCAGTCGTTCAAAAAGAAACACCAGTT
GAAGAGAAAGAAACTCGTGATAAAGTTGATGAAGATAGAAAACATCAAATTGAAGCTTCC
ATCGTTCGTATTATGAAAGCTCGTAAAACACTTGAACACTCTAATCTTGTCTCTGAAGTC
ATCAAGCAACTTCAATCTCGTTTTGTTCCAAATCCAGTCATTGTAAAGAAACGTATCGAA
TCTTTAATGAAAGAGA