>AFH266 (AFH266Q) /CSM/AF/AFH2-C/AFH266Q.Seq.d/
XXXXXXXXXXTGAAAAAGAAACCGGACTTGATATACCAATTCATGTCGATGCAGCAAGTG
GTGGTTTCGTTGCACCCTTTCTTCAACCAGAAATTGTTTGGGATTTTAGACTACCACGTG
TTAAATCAATTAATGGCTCTGGTCATAAATTTGGTCTATCTCCATTGGGAGTTGGCTGGG
TCGTATGGAGAGGTAAAAATGACATTCACAAGGATTTGGTATTCGATGTCAACTATCTTG
GTGGCAATATGCCAACATTTTCATTGAATTTCTCACGTCCAGGTGGTCAAATAGTATGCC
AGTACTATAACTTTTTGCGCCATGGTCGTAGTGGATATACCGCAGTTCATCAAGCTTGTC
TAGATCATGGTATTTTCATTTCCAAGCAAGTTAAAAAACACGGTATTTTTGATATTGTTT
ATGATGGTACTGGTGCACTTCCAGGTGCATGTTGGAAATTGAAAAAAAGATGCAAAAGTA
AAATTCAATCTCTTTGACCTTTCAGATAGAATGAGAAATAGAGGTTGGCAAATTGCATCC
TATACTCTACCAAACTTACCAGATATTGTAGTTCAC

---EKETGLDIPIHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKFGLSPLGVGWV
VWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRSGYTAVHQACL
DHGIFISKQVKKHGIFDIVYDGTGALPGACWKLKKRCKSKIQSL*pfr*nek*rlancil
ystkltrycss

Frame A:
---EKETGLDIPIHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKFGLSPLGVGWV
VWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRSGYTAVHQACL
DHGIFISKQVKKHGIFDIVYDGTGALPGACWKLKKRCKSKIQSL*pfr*nek*rlancil
ystkltrycss


Frame B:
---kkkpdliyqfmsmqqvvvslhpffnqklfgildyhvlnqlmalvinlvylhwelags
ygevkmtftriwysmstilvaicqhfh*ishvqvvk*yastitfcamvvvdipqfiklv*
imvfsfpsklkntvflilfmmvlvhfqvhvgn*kkdakvkfnlfdlsdrmrnrgwqiasy
tlpnlpdivvh


Frame C:
---krnrt*ytnscrcskwwfrctlsstrnclgf*tttc*in*wlws*iwsisigswlgr
mer*k*hsqgfgircqlswwqyanifiefltsrwsnsmpvl*lfapws*wiyrsssslsr
swyfhfqas*ktryf*ycl*wywctsrcmleiekkmqk*nsisltfqie*eievgklhpi
lyqtyqil*f

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH266 (AFH266Q) /CSM/AF/AFH2-C/AFH266Q.Seq.d/                       1080   0.0
AFH873 (AFH873Q) /CSM/AF/AFH8-D/AFH873Q.Seq.d/                       1078   0.0
SFH378 (SFH378Q) /CSM/SF/SFH3-D/SFH378Q.Seq.d/                       1068   0.0
SFG840 (SFG840Q) /CSM/SF/SFG8-B/SFG840Q.Seq.d/                       1068   0.0
SFG754 (SFG754Q) /CSM/SF/SFG7-C/SFG754Q.Seq.d/                       1068   0.0
SFE561 (SFE561Q) /CSM/SF/SFE5-C/SFE561Q.Seq.d/                       1068   0.0
SFD593 (SFD593Q) /CSM/SF/SFD5-D/SFD593Q.Seq.d/                       1068   0.0
SFD334 (SFD334Q) /CSM/SF/SFD3-B/SFD334Q.Seq.d/                       1068   0.0
SFC434 (SFC434Q) /CSM/SF/SFC4-B/SFC434Q.Seq.d/                       1068   0.0
SFC433 (SFC433Q) /CSM/SF/SFC4-B/SFC433Q.Seq.d/                       1068   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AR375678|AR375678.1 Sequence 684 from patent US 6605709.           56   4e-12  3
BG138757|BG138757.1 EST479199 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP11A1 5' sequence, mRNA sequence.    66   8e-10  2
CF811515|CF811515.2 NA716 cDNA non acclimated Bluecrop library Vaccinium corymbosum cDNA 5', mRNA sequence.    70   6e-09  2
AF352732|AF352732.1 Nicotiana tabacum glutamate decarboxylase isozyme 3 mRNA, complete cds.    58   2e-07  2
CK273593|CK273593.1 EST719671 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADC22 5' end, mRNA sequence.    50   2e-07  2
CD008053|CD008053.1 VVB082D05_335234 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB082D05 5, mRNA sequence.    64   4e-07  2
CD006712|CD006712.1 VVB066D06_332552 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB066D06 5, mRNA sequence.    64   4e-07  2
CD719214|CD719214.1 VVB158G03_410335 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB158G03 5, mRNA sequence.    64   4e-07  2
CA813481|CA813481.1 CA48LN10IF-E8 Cabernet Sauvignon Leaf - CA48LN Vitis vinifera cDNA clone CA48LN10IF-E8 5', mRNA sequence.    64   5e-07  2
CB341828|CB341828.2 CA32EN0002_IIIbF_E01 Cabernet Sauvignon Leaf - CA32EN Vitis vinifera cDNA clone CA32EN0002_IIIbF_E01 5', mRNA sequence.    64   5e-07  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM178379_1197(FM178379|pid:none) Aliivibrio salmonicida LFI1238 ...   244   1e-70
CP000020_1061(CP000020|pid:none) Vibrio fischeri ES114 chromosom...   241   9e-70
CP000789_2953(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 ch...   236   1e-68
CP000851_2941(CP000851|pid:none) Shewanella pealeana ATCC 700345...   236   3e-68
CP001358_45(CP001358|pid:none) Desulfovibrio desulfuricans subsp...   234   1e-67
CR522870_385(CR522870|pid:none) Desulfotalea psychrophila LSv54 ...   224   1e-65
AM942759_1390(AM942759|pid:none) Proteus mirabilis strain HI4320...   222   2e-64
CP000439_1647(CP000439|pid:none) Francisella tularensis subsp. n...   226   2e-64
CP000608_56(CP000608|pid:none) Francisella tularensis subsp. tul...   223   2e-63
AY967181_1(AY967181|pid:none) Synthetic construct isolate FTT172...   220   2e-62

 psg: 0.75  gvh: 0.40  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFH266 is cyt

>AFH266Z.Seq
----------TGAAAAAGAAACCGGACTTGATATACCAATTCATGTCGATGCAGCAAGTG
GTGGTTTCGTTGCACCCTTTCTTCAACCAGAAATTGTTTGGGATTTTAGACTACCACGTG
TTAAATCAATTAATGGCTCTGGTCATAAATTTGGTCTATCTCCATTGGGAGTTGGCTGGG
TCGTATGGAGAGGTAAAAATGACATTCACAAGGATTTGGTATTCGATGTCAACTATCTTG
GTGGCAATATGCCAACATTTTCATTGAATTTCTCACGTCCAGGTGGTCAAATAGTATGCC
AGTACTATAACTTTTTGCGCCATGGTCGTAGTGGATATACCGCAGTTCATCAAGCTTGTC
TAGATCATGGTATTTTCATTTCCAAGCAAGTTAAAAAACACGGTATTTTTGATATTGTTT
ATGATGGTACTGGTGCACTTCCAGGTGCATGTTGGAAATTGAAAAAAAGATGCAAAAGTA
AAATTCAATCTCTTTGACCTTTCAGATAGAATGAGAAATAGAGGTTGGCAAATTGCATCC
TATACTCTACCAAACTTACCAGATATTGTAGTTCAC