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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH258
Library AF
(Link to library)
Clone ID AFH258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14990-1
Original site URL
Representative seq. ID AFH258P
(Link to Original site)
Representative DNA sequence 
>AFH258 (AFH258Q) /CSM/AF/AFH2-C/AFH258Q.Seq.d/
TGGCCTACTGGNAAGTAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGT
GGAAATGGTAGTGATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAA
CCACCAAAAAAAACAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATA
ACATCATCATTAAAGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCA
TCATCATCATGTTGTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGT
AGTGGTGGTGAAAGTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAA
GAAAAACAAAAATCAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGT
AGTAGTAATAATAATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGT
GAAAAAAGTGGATCATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACA
AATAAACAAATANAAGATGATTCAAATATTTTTATCAAGTGGTGCAACAATAATTGAAGG
TAATATTATTAAAGCATNANNTGGATGTCXXXXXXXXXXGAAATAATAACACCACCAAAG
TATCTGTATTAGATGTTGATATTTGCGGTCCGTCAATTCCAAAGCTAATGGGTGTTGATA
AATTGCAAATTATAAATTCAGAGTATGGTTGGATTCCACCAAAGGTGCAACAAGCAAATC
ATGATATTAAAGTCATGTCTGTTGGTTTTTTATTGGGTACCCCTGATGCACCTGTAATTT
GGAAAGGTCCAAGAAAGACCACAATGATTCGTAGATTCCTTAAAGATACATTTTGGGGTA
AACAAGATTATCTCATCATCGATACGCCACCAGGCACAAGTGATGAACATCTCTCCATTA
TCAATTCTCTAAAATCTTGTAATCCCGATGGTGCAGTATTGGTCACCACTCCACAAGATC
TCTCTTGTGATACTGTAAAAAAAGAGATTTCATTATGTCGTCAATTAAATGTACCAATCA
TAGGTATCATTGAAAATTTATCTGGTTTTGTATGTCCATGTTGTGATGAAGTAACAGAAA
TTTTCAAATCTGATGGTGGTAAAAAGTTGGCAGAACAATTTAATATTCCATTCCTTGGCA
AAATTCCAATAGATACAAATTTAGGTAAATCAGCTGAAAATGGGGTTTGTTCAATTTGTG
ATCATCCAAATACTCCTGGTACTTTAGCAATTCATTCAATTATTTATAAAATNATCAAAT
AATAATTAAAATAAAAAAAAACTTTAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
gllxskinikqi*k*kcqvvemvvii*kilaiilkkllfnhqkkqlql*ynkilmilkq*
hhh*SQNPLVAVLVKINHHHHHHVVVVVIKLKKNKNQVVVVVVKVNHHHHVVVVIIINLK
KNKNQVVVEEMKINHHLHVVVVIIIIKLKKNKNQVVVEVVKKVDHHHVVNQNQRQQQQQQ
INKXKMIQIFLSSGATIIEGNIIKAXXGC---

---NNNTTKVSVLDVDICGPSIPKLMGVDKLQIINSEYGWIPPKVQQANHDIKVMSVGFL
LGTPDAPVIWKGPRKTTMIRRFLKDTFWGKQDYLIIDTPPGTSDEHLSIINSLKSCNPDG
AVLVTTPQDLSCDTVKKEISLCRQLNVPIIGIIENLSGFVCPCCDEVTEIFKSDGGKKLA
EQFNIPFLGKIPIDTNLGKSAENGVCSICDHPNTPGTLAIHSIIYKXIK**lk*kktl
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgk*n*y*tnikikmsgcgngsdnledignnfkkvvvqppkkttsiiiqqdsddsqti
tsslkpkstccsagenkssssssccsssnkveekqkssccsggeskssssccsgnnnkfe
ekqkssccggnenkssssccssnnnnkveekqksgccggseksgsssccksesttttttt
nkqixddsnifikwcnnn*r*yy*sxxwm---

---eiitppkyly*mlifavrqfqs*wvlinckl*iqsmvgfhqrcnkqimilkscllvf
ywvplmhl*fgkvqerpq*fvdslkihfgvnkiisssirhqaqvmnisplsil*nlvipm
vqywsplhkislvil*kkrfhyvvn*myqs*vslkiylvlyvhvvmk*qkfsnlmvvksw
qnnlifhslakfq*iqi*vnqlkmgfvqfviiqillvl*qfiqlfikxsnnn*nkkkl*

Frame B:
gllxskinikqi*k*kcqvvemvvii*kilaiilkkllfnhqkkqlql*ynkilmilkq*
hhh*SQNPLVAVLVKINHHHHHHVVVVVIKLKKNKNQVVVVVVKVNHHHHVVVVIIINLK
KNKNQVVVEEMKINHHLHVVVVIIIIKLKKNKNQVVVEVVKKVDHHHVVNQNQRQQQQQQ
INKXKMIQIFLSSGATIIEGNIIKAXXGC---

---k**hhqsicirc*ylrsvnskangc**ianykfrvwldstkgatsks*y*shvcwff
igyp*ctcnlerskkdhnds*ip*ryilg*trlshhryatrhk**tslhyqfskil*srw
csighhstrsll*yckkrdfimssikctnhryh*kfiwfcmsml**snrnfqi*ww*kvg
rti*ysipwqnsnrykfr*is*kwglfnl*sskyswyfsnsfnyl*nxqiiikikknfk

Frame C:
aywxvklilnkyknknvrlwkw***frrywq*f*ksccsttkknnfnynttrf**fsnnn
iiikakihllqcw*k*iiiiiiml*****s*rktkikll*ww*k*iiiiml*w****i*r
ktkikllwrk*k*iiifml*******s*rktkirllwr**kkwiiiml*irindnnnnnk
*tnxr*fkyfyqvvqq*lkvillkhxxdv---

---NNNTTKVSVLDVDICGPSIPKLMGVDKLQIINSEYGWIPPKVQQANHDIKVMSVGFL
LGTPDAPVIWKGPRKTTMIRRFLKDTFWGKQDYLIIDTPPGTSDEHLSIINSLKSCNPDG
AVLVTTPQDLSCDTVKKEISLCRQLNVPIIGIIENLSGFVCPCCDEVTEIFKSDGGKKLA
EQFNIPFLGKIPIDTNLGKSAENGVCSICDHPNTPGTLAIHSIIYKXIK**lk*kktl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH258 (AFH258Q) /CSM/AF/AFH2-C/AFH258Q.Seq.d/                       1415   0.0
AFH315 (AFH315Q) /CSM/AF/AFH3-A/AFH315Q.Seq.d/                       1304   0.0
VFL193 (VFL193Q) /CSM/VF/VFL1-D/VFL193Q.Seq.d/                       1217   0.0
VFC456 (VFC456Q) /CSM/VF/VFC4-C/VFC456Q.Seq.d/                       1168   0.0
AFH252 (AFH252Q) /CSM/AF/AFH2-C/AFH252Q.Seq.d/                       1080   0.0
VSC152 (VSC152Q) /CSM/VS/VSC1-C/VSC152Q.Seq.d/                         50   9e-05
VHH281 (VHH281Q) /CSM/VH/VHH2-D/VHH281Q.Seq.d/                         50   9e-05
SSE358 (SSE358Q) /CSM/SS/SSE3-C/SSE358Q.Seq.d/                         50   9e-05
SSD430 (SSD430Q) /CSM/SS/SSD4-B/SSD430Q.Seq.d/                         50   9e-05
SFL154 (SFL154Q) /CSM/SF/SFL1-C/SFL154Q.Seq.d/                         50   9e-05
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014820|AE014820.1 Plasmodium falciparum 3D7 chromosome 14 section 5 of 13 of the complete sequence.    42   7e-10  12
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  42   1e-09  14
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    44   1e-09  10
AC116919|AC116919.2 Dictyostelium discoideum chromosome 2 map complement(6245035-6206343) strain AX4, complete sequence.    44   1e-09  12
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           44   1e-09  12
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    40   2e-09  14
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    48   6e-09  6
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   8e-09  7
AE014819|AE014819.1 Plasmodium falciparum 3D7 chromosome 14 section 4 of 13 of the complete sequence.    46   2e-08  15
AL929351|AL929351.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 1/4.    42   2e-08  16
dna update 2004. 4. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NZ7) RecName: Full=Nucleotide-binding protein 2 homolog;    ...   248   4e-64
BC064668_1(BC064668|pid:none) Danio rerio nucleotide binding pro...   241   3e-62
BC093447_1(BC093447|pid:none) Danio rerio nucleotide binding pro...   241   3e-62
AK223204_1(AK223204|pid:none) Homo sapiens mRNA for nucleotide b...   237   6e-61
BC109323_1(BC109323|pid:none) Homo sapiens nucleotide binding pr...   237   6e-61
BC088708_1(BC088708|pid:none) Xenopus laevis hypothetical LOC496...   237   6e-61
BC094205_1(BC094205|pid:none) Xenopus laevis hypothetical LOC494...   236   2e-60
BC090123_1(BC090123|pid:none) Xenopus tropicalis MGC97800 protei...   236   2e-60
(P53384) RecName: Full=Nucleotide-binding protein 1;          Sh...   235   2e-60
BC106243_1(BC106243|pid:none) Xenopus laevis hypothetical LOC494...   235   3e-60
protein update 2009. 4.21
PSORT 

 psg: 0.86  gvh: 0.16  alm: 0.36  top: 0.73  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for AFH258 is nuc
5' end seq. ID AFH258F
5' end seq. 
>AFH258F.Seq
TGGCCTACTGGNAAGTAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGT
GGAAATGGTAGTGATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAA
CCACCAAAAAAAACAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATA
ACATCATCATTAAAGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCA
TCATCATCATGTTGTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGT
AGTGGTGGTGAAAGTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAA
GAAAAACAAAAATCAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGT
AGTAGTAATAATAATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGT
GAAAAAAGTGGATCATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACA
AATAAACAAATANAAGATGATTCAAATATTTTTATCAAGTGGTGCAACAATAATTGAAGG
TAATATTATTAAAGCATNANNTGGATGTC----------
Length of 5' end seq. 629
3' end seq. ID AFH258Z
3' end seq. 
>AFH258Z.Seq
----------GAAATAATAACACCACCAAAGTATCTGTATTAGATGTTGATATTTGCGGT
CCGTCAATTCCAAAGCTAATGGGTGTTGATAAATTGCAAATTATAAATTCAGAGTATGGT
TGGATTCCACCAAAGGTGCAACAAGCAAATCATGATATTAAAGTCATGTCTGTTGGTTTT
TTATTGGGTACCCCTGATGCACCTGTAATTTGGAAAGGTCCAAGAAAGACCACAATGATT
CGTAGATTCCTTAAAGATACATTTTGGGGTAAACAAGATTATCTCATCATCGATACGCCA
CCAGGCACAAGTGATGAACATCTCTCCATTATCAATTCTCTAAAATCTTGTAATCCCGAT
GGTGCAGTATTGGTCACCACTCCACAAGATCTCTCTTGTGATACTGTAAAAAAAGAGATT
TCATTATGTCGTCAATTAAATGTACCAATCATAGGTATCATTGAAAATTTATCTGGTTTT
GTATGTCCATGTTGTGATGAAGTAACAGAAATTTTCAAATCTGATGGTGGTAAAAAGTTG
GCAGAACAATTTAATATTCCATTCCTTGGCAAAATTCCAATAGATACAAATTTAGGTAAA
TCAGCTGAAAATGGGGTTTGTTCAATTTGTGATCATCCAAATACTCCTGGTACTTTAGCA
ATTCATTCAATTATTTATAAAATNATCAAATAATAATTAAAATAAAAAAAAACTTTAAA
Length of 3' end seq. 709
Connected seq. ID AFH258P
Connected seq. 
>AFH258P.Seq
TGGCCTACTGGNAAGTAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGT
GGAAATGGTAGTGATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAA
CCACCAAAAAAAACAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATA
ACATCATCATTAAAGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCA
TCATCATCATGTTGTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGT
AGTGGTGGTGAAAGTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAA
GAAAAACAAAAATCAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGT
AGTAGTAATAATAATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGT
GAAAAAAGTGGATCATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACA
AATAAACAAATANAAGATGATTCAAATATTTTTATCAAGTGGTGCAACAATAATTGAAGG
TAATATTATTAAAGCATNANNTGGATGTC----------GAAATAATAACACCACCAAAG
TATCTGTATTAGATGTTGATATTTGCGGTCCGTCAATTCCAAAGCTAATGGGTGTTGATA
AATTGCAAATTATAAATTCAGAGTATGGTTGGATTCCACCAAAGGTGCAACAAGCAAATC
ATGATATTAAAGTCATGTCTGTTGGTTTTTTATTGGGTACCCCTGATGCACCTGTAATTT
GGAAAGGTCCAAGAAAGACCACAATGATTCGTAGATTCCTTAAAGATACATTTTGGGGTA
AACAAGATTATCTCATCATCGATACGCCACCAGGCACAAGTGATGAACATCTCTCCATTA
TCAATTCTCTAAAATCTTGTAATCCCGATGGTGCAGTATTGGTCACCACTCCACAAGATC
TCTCTTGTGATACTGTAAAAAAAGAGATTTCATTATGTCGTCAATTAAATGTACCAATCA
TAGGTATCATTGAAAATTTATCTGGTTTTGTATGTCCATGTTGTGATGAAGTAACAGAAA
TTTTCAAATCTGATGGTGGTAAAAAGTTGGCAGAACAATTTAATATTCCATTCCTTGGCA
AAATTCCAATAGATACAAATTTAGGTAAATCAGCTGAAAATGGGGTTTGTTCAATTTGTG
ATCATCCAAATACTCCTGGTACTTTAGCAATTCATTCAATTATTTATAAAATNATCAAAT
AATAATTAAAATAAAAAAAAACTTTAAA
Length of connected seq. 1338
Full length Seq ID -
Full length Seq. -
Length of full length seq. -