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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH252
Library AF
(Link to library)
Clone ID AFH252
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14990-1
Original site URL
Representative seq. ID AFH252P
(Link to Original site)
Representative DNA sequence 
>AFH252 (AFH252Q) /CSM/AF/AFH2-C/AFH252Q.Seq.d/
AAGAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGTGGAAATGGTAGTG
ATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAACCACCAAAAAAAA
CAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATAACATCATCATTAA
AGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCATCATCATCATGTT
GTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGTAGTGGTGGTGAAA
GTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAAGAAAAACAAAAAT
CAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGTAGTAGTAATAATA
ATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGTGAAAAAAGTGGAT
CATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACAAATAAACAAATAA
AAGATGATTCAAATATTTTATCAAGTGGTGCAACAATAATTGAAGGTAATATTATTAAAG
CATCAGTTGGATGTCCTTCAGATACACCATTAGCAGGATCAGAAGCAATTTGTTCAAGTT
GTCCAGGTCAAAATGCATGTAAATCACAAGCTGATAATCCTGATAAAAAGAGTATTGAAA
TTAGAATGAAAGTTATTAAAAATAAGATATTGGTAATGTCATCAAAGGGTGGTGTTGGTA
AATCAACAGTTTCATCCTTGTTATCATATGGTTTTTCAAAGAGAAATAATAACACCACCA
AAGTATCTGTATTAGATGTTGATATTTGCGGTCCGTCAATTCCAAAGCTAATGGGTGTTG
ATAAATTGCAAATTATAAATTCAGAGTATGGTTGGATTCCACCAAAGGTGCAACAAGCAA
ATCATGATATTAAAGTCATGTCTGTTGXXXXXXXXXXAAAGGTGCAACAAGCAAATCATG
ATATTAAAGTCATGTCTGTTGGTTTTTTATTGGGTACCCCTGATGCACCTGTAATTTGGA
AAGGTCCAAGAAAGACCACAATGATTCGTAGATTCCTTAAAGATACATTTTGGGGTAAAC
AAGATTATCTCATCATCGATACGCCACCAGGCACAAGTGATGAACATCTCTCCATTATCA
ATTCTCTAAAATCTTGTAATCCCGATGGTGCAGTATTGGTCACCACTCCACAAGATCTCT
CTTGTGATACTGTAAAAAAAGAGATTTCATTATGTCGTCAATTAAATGTACCAATCATAG
GTATCATTGAAAATTTATCTGGTTTTGTATGTCCATGTTGTGATGAAGTAACAGAAATTT
TCAAATCTGATGGTGGTAAAAAGTTGGCAGAACAATTTAATATTCCATTCCTTGGCAAAA
TTCCAATAGATACAAATTTAGGTAAATCAGCTGAAAATGGGGTTTGTTCAATTTGTGATC
ATCCAAATACTCCTGGTACTTTAGCAATTCATTCAATTATTNATAAAATNATCAAATAAT
AATTAAAATAAAAAAAAACTTTAAA
sequence update 2003. 5. 2
Translated Amino Acid sequence 
en*y*TNIKIKMSGCGNGSDNLEDIGNNFKKVVVQPPKKTTSIIIQQDSDDSQTITSSLK
PKSTCCSAGENKSSSSSSCCSSSNKVEEKQKSSCCSGGESKSSSSCCSGNNNKFEEKQKS
SCCGGNENKSSSSCCSSNNNNKVEEKQKSGCCGGSEKSGSSSCCKSESTTTTTTTNKQIK
DDSNILSSGATIIEGNIIKASVGCPSDTPLAGSEAICSSCPGQNACKSQADNPDKKSIEI
RMKVIKNKILVMSSKGGVGKSTVSSLLSYGFSKRNNNTTKVSVLDVDICGPSIPKLMGVD
KLQIINSEYGWIPPKVQQANHDIKVMSV---

---KVQQANHDIKVMSVGFLLGTPDAPVIWKGPRKTTMIRRFLKDTFWGKQDYLIIDTPP
GTSDEHLSIINSLKSCNPDGAVLVTTPQDLSCDTVKKEISLCRQLNVPIIGIIENLSGFV
CPCCDEVTEIFKSDGGKKLAEQFNIPFLGKIPIDTNLGKSAENGVCSICDHPNTPGTLAI
HSIIXKXIK**lk*kktl
Translated Amino Acid sequence (All Frames) 
Frame A:
kkinikqi*k*kcqvvemvvii*kilaiilkkllfnhqkkqlql*ynkilmilkq*hhh*
sqnplvavlvkinhhhhhhvvvvviklkknknqvvvvvvkvnhhhhvvvviiinlkknkn
qvvveemkinhhlhvvvviiiiklkknknqvvvevvkkvdhhhvvnqnqrqqqqqqink*
kmiqifyqvvqq*lkvillkhqldvlqihh*qdqkqfvqvvqvkmhvnhkliilikrvlk
le*kllkiryw*chqrvvlvnqqfhpcyhmvfqreiitppkyly*mlifavrqfqs*wvl
inckl*iqsmvgfhqrcnkqimilkscll---

---kgatsks*y*shvcwffigyp*ctcnlerskkdhnds*ip*ryilg*trlshhryat
rhk**tslhyqfskil*srwcsighhstrsll*yckkrdfimssikctnhryh*kfiwfc
msml**snrnfqi*ww*kvgrti*ysipwqnsnrykfr*is*kwglfnl*sskyswyfsn
sfnyx*nxqiiikikknfk

Frame B:
rklilnkyknknvrlwkw***frrywq*f*ksccsttkknnfnynttrf**fsnnniiik
akihllqcw*k*iiiiiiml*****s*rktkikll*ww*k*iiiiml*w****i*rktki
kllwrk*k*iiifml*******s*rktkirllwr**kkwiiiml*irindnnnnnk*tnk
r*fkyfikwcnnn*r*yy*siswmsfrytisrirsnlfklsrskcm*its**s**key*n
*nesy*k*dignvikgwcw*insfilviiwffkek**hhqsicirc*ylrsvnskangc*
*ianykfrvwldstkgatsks*y*shvc---

---KVQQANHDIKVMSVGFLLGTPDAPVIWKGPRKTTMIRRFLKDTFWGKQDYLIIDTPP
GTSDEHLSIINSLKSCNPDGAVLVTTPQDLSCDTVKKEISLCRQLNVPIIGIIENLSGFV
CPCCDEVTEIFKSDGGKKLAEQFNIPFLGKIPIDTNLGKSAENGVCSICDHPNTPGTLAI
HSIIXKXIK**lk*kktl

Frame C:
en*y*TNIKIKMSGCGNGSDNLEDIGNNFKKVVVQPPKKTTSIIIQQDSDDSQTITSSLK
PKSTCCSAGENKSSSSSSCCSSSNKVEEKQKSSCCSGGESKSSSSCCSGNNNKFEEKQKS
SCCGGNENKSSSSCCSSNNNNKVEEKQKSGCCGGSEKSGSSSCCKSESTTTTTTTNKQIK
DDSNILSSGATIIEGNIIKASVGCPSDTPLAGSEAICSSCPGQNACKSQADNPDKKSIEI
RMKVIKNKILVMSSKGGVGKSTVSSLLSYGFSKRNNNTTKVSVLDVDICGPSIPKLMGVD
KLQIINSEYGWIPPKVQQANHDIKVMSV---

---rcnkqimilkscllvfywvplmhl*fgkvqerpq*fvdslkihfgvnkiisssirhq
aqvmnisplsil*nlvipmvqywsplhkislvil*kkrfhyvvn*myqs*vslkiylvly
vhvvmk*qkfsnlmvvkswqnnlifhslakfq*iqi*vnqlkmgfvqfviiqillvl*qf
iqlxikxsnnn*nkkkl*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH252 (AFH252Q) /CSM/AF/AFH2-C/AFH252Q.Seq.d/                       1951   0.0
AFH258 (AFH258Q) /CSM/AF/AFH2-C/AFH258Q.Seq.d/                       1080   0.0
VFL193 (VFL193Q) /CSM/VF/VFL1-D/VFL193Q.Seq.d/                       1074   0.0
AFH315 (AFH315Q) /CSM/AF/AFH3-A/AFH315Q.Seq.d/                       1059   0.0
VFC456 (VFC456Q) /CSM/VF/VFC4-C/VFC456Q.Seq.d/                       1031   0.0
VSC152 (VSC152Q) /CSM/VS/VSC1-C/VSC152Q.Seq.d/                         50   1e-04
VHH281 (VHH281Q) /CSM/VH/VHH2-D/VHH281Q.Seq.d/                         50   1e-04
SSE358 (SSE358Q) /CSM/SS/SSE3-C/SSE358Q.Seq.d/                         50   1e-04
SSD430 (SSD430Q) /CSM/SS/SSD4-B/SSD430Q.Seq.d/                         50   1e-04
SFL154 (SFL154Q) /CSM/SF/SFL1-C/SFL154Q.Seq.d/                         50   1e-04
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  42   9e-10  15
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    46   1e-09  8
AE014820|AE014820.1 Plasmodium falciparum 3D7 chromosome 14 section 5 of 13 of the complete sequence.    42   2e-09  13
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           44   2e-09  12
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    44   4e-09  10
AC116919|AC116919.2 Dictyostelium discoideum chromosome 2 map complement(6245035-6206343) strain AX4, complete sequence.    44   6e-09  12
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    42   8e-09  11
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    48   1e-08  6
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    48   8e-08  18
AC116989|AC116989.3 Dictyostelium discoideum chromosome 2 map 4545040-4559643 strain AX4, complete sequence.    44   1e-07  6
dna update 2004. 4. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC109323_1(BC109323|pid:none) Homo sapiens nucleotide binding pr...   254   8e-66
AK223204_1(AK223204|pid:none) Homo sapiens mRNA for nucleotide b...   252   2e-65
BC055436_1(BC055436|pid:none) Mus musculus nucleotide binding pr...   252   3e-65
(P53384) RecName: Full=Nucleotide-binding protein 1;          Sh...   252   3e-65
(Q9R060) RecName: Full=Nucleotide-binding protein 1;          Sh...   251   4e-65
BC064668_1(BC064668|pid:none) Danio rerio nucleotide binding pro...   251   4e-65
BC093447_1(BC093447|pid:none) Danio rerio nucleotide binding pro...   251   4e-65
(Q5I0L4) RecName: Full=Nucleotide binding protein 1;          Sh...   250   8e-65
BC094205_1(BC094205|pid:none) Xenopus laevis hypothetical LOC494...   250   1e-64
BC088708_1(BC088708|pid:none) Xenopus laevis hypothetical LOC496...   249   1e-64
protein update 2009. 3.28
PSORT 

 psg: 0.80  gvh: 0.38  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFH252 is cyt
5' end seq. ID AFH252F
5' end seq. 
>AFH252F.Seq
AAGAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGTGGAAATGGTAGTG
ATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAACCACCAAAAAAAA
CAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATAACATCATCATTAA
AGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCATCATCATCATGTT
GTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGTAGTGGTGGTGAAA
GTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAAGAAAAACAAAAAT
CAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGTAGTAGTAATAATA
ATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGTGAAAAAAGTGGAT
CATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACAAATAAACAAATAA
AAGATGATTCAAATATTTTATCAAGTGGTGCAACAATAATTGAAGGTAATATTATTAAAG
CATCAGTTGGATGTCCTTCAGATACACCATTAGCAGGATCAGAAGCAATTTGTTCAAGTT
GTCCAGGTCAAAATGCATGTAAATCACAAGCTGATAATCCTGATAAAAAGAGTATTGAAA
TTAGAATGAAAGTTATTAAAAATAAGATATTGGTAATGTCATCAAAGGGTGGTGTTGGTA
AATCAACAGTTTCATCCTTGTTATCATATGGTTTTTCAAAGAGAAATAATAACACCACCA
AAGTATCTGTATTAGATGTTGATATTTGCGGTCCGTCAATTCCAAAGCTAATGGGTGTTG
ATAAATTGCAAATTATAAATTCAGAGTATGGTTGGATTCCACCAAAGGTGCAACAAGCAA
ATCATGATATTAAAGTCATGTCTGTTGNNNNNNNNNN
Length of 5' end seq. 997
3' end seq. ID AFH252Z
3' end seq. 
>AFH252Z.Seq
----------AAAGGTGCAACAAGCAAATCATGATATTAAAGTCATGTCTGTTGGTTTTT
TATTGGGTACCCCTGATGCACCTGTAATTTGGAAAGGTCCAAGAAAGACCACAATGATTC
GTAGATTCCTTAAAGATACATTTTGGGGTAAACAAGATTATCTCATCATCGATACGCCAC
CAGGCACAAGTGATGAACATCTCTCCATTATCAATTCTCTAAAATCTTGTAATCCCGATG
GTGCAGTATTGGTCACCACTCCACAAGATCTCTCTTGTGATACTGTAAAAAAAGAGATTT
CATTATGTCGTCAATTAAATGTACCAATCATAGGTATCATTGAAAATTTATCTGGTTTTG
TATGTCCATGTTGTGATGAAGTAACAGAAATTTTCAAATCTGATGGTGGTAAAAAGTTGG
CAGAACAATTTAATATTCCATTCCTTGGCAAAATTCCAATAGATACAAATTTAGGTAAAT
CAGCTGAAAATGGGGTTTGTTCAATTTGTGATCATCCAAATACTCCTGGTACTTTAGCAA
TTCATTCAATTATTNATAAAATNATCAAATAATAATTAAAATAAAAAAAAACTTTAAA
Length of 3' end seq. 588
Connected seq. ID AFH252P
Connected seq. 
>AFH252P.Seq
AAGAAAATTAATATTAAACAAATATAAAAATAAAAATGTCAGGTTGTGGAAATGGTAGTG
ATAATTTAGAAGATATTGGCAATAATTTTAAAAAAGTTGTTGTTCAACCACCAAAAAAAA
CAACTTCAATTATAATACAACAAGATTCTGATGATTCTCAAACAATAACATCATCATTAA
AGCCAAAATCCACTTGTTGCAGTGCTGGTGAAAATAAATCATCATCATCATCATCATGTT
GTAGTAGTAGTAATAAAGTTGAAGAAAAACAAAAATCAAGTTGTTGTAGTGGTGGTGAAA
GTAAATCATCATCATCATGTTGTAGTGGTAATAATAATAAATTTGAAGAAAAACAAAAAT
CAAGTTGTTGTGGAGGAAATGAAAATAAATCATCATCTTCATGTTGTAGTAGTAATAATA
ATAATAAAGTTGAAGAAAAACAAAAATCAGGTTGTTGTGGAGGTAGTGAAAAAAGTGGAT
CATCATCATGTTGTAAATCAGAATCAACGACAACAACAACAACAACAAATAAACAAATAA
AAGATGATTCAAATATTTTATCAAGTGGTGCAACAATAATTGAAGGTAATATTATTAAAG
CATCAGTTGGATGTCCTTCAGATACACCATTAGCAGGATCAGAAGCAATTTGTTCAAGTT
GTCCAGGTCAAAATGCATGTAAATCACAAGCTGATAATCCTGATAAAAAGAGTATTGAAA
TTAGAATGAAAGTTATTAAAAATAAGATATTGGTAATGTCATCAAAGGGTGGTGTTGGTA
AATCAACAGTTTCATCCTTGTTATCATATGGTTTTTCAAAGAGAAATAATAACACCACCA
AAGTATCTGTATTAGATGTTGATATTTGCGGTCCGTCAATTCCAAAGCTAATGGGTGTTG
ATAAATTGCAAATTATAAATTCAGAGTATGGTTGGATTCCACCAAAGGTGCAACAAGCAA
ATCATGATATTAAAGTCATGTCTGTTG----------AAAGGTGCAACAAGCAAATCATG
ATATTAAAGTCATGTCTGTTGGTTTTTTATTGGGTACCCCTGATGCACCTGTAATTTGGA
AAGGTCCAAGAAAGACCACAATGATTCGTAGATTCCTTAAAGATACATTTTGGGGTAAAC
AAGATTATCTCATCATCGATACGCCACCAGGCACAAGTGATGAACATCTCTCCATTATCA
ATTCTCTAAAATCTTGTAATCCCGATGGTGCAGTATTGGTCACCACTCCACAAGATCTCT
CTTGTGATACTGTAAAAAAAGAGATTTCATTATGTCGTCAATTAAATGTACCAATCATAG
GTATCATTGAAAATTTATCTGGTTTTGTATGTCCATGTTGTGATGAAGTAACAGAAATTT
TCAAATCTGATGGTGGTAAAAAGTTGGCAGAACAATTTAATATTCCATTCCTTGGCAAAA
TTCCAATAGATACAAATTTAGGTAAATCAGCTGAAAATGGGGTTTGTTCAATTTGTGATC
ATCCAAATACTCCTGGTACTTTAGCAATTCATTCAATTATTNATAAAATNATCAAATAAT
AATTAAAATAAAAAAAAACTTTAAA
Length of connected seq. 1575
Full length Seq ID -
Full length Seq. -
Length of full length seq. -