NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH247
Library AF
(Link to library)
Clone ID AFH247
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFH247Z
(Link to Original site)
Representative DNA sequence 
>AFH247 (AFH247Q) /CSM/AF/AFH2-B/AFH247Q.Seq.d/
XXXXXXXXXXAAGAGCACGTTTAGGTACTCATACTGGATTTGTAAATCAGTTAGTTACAC
CATATCATGTTGATATTAAATGTACAGTTAAATCAGAAATTTCAAAAGAGATTGTAGAGG
AAGCCGTTGCAAAAGCATCAGGTAAAGCATCAATGGTATTGAGTGATGATGCTCAATCAC
GTGGTAGTGGTGGTCGTATGACCTCAAGTTACAGTTCATCAAGTGCTTCAACTCAAGGTT
CAAACACAACCGGTAAAACATTTGCTGGTGCTGGTAGTAAATTTACAACACCATCAGCTG
TGCCAAAATCAAGTGGTTCAACACTTGGTTTCGATGATGAACAACAAGTTAGAGATACAA
TCAAAGATGTTAGATCCGATAGTACTGACACTAATTGGTGTTTAGTTGGTTATAAAAATG
ATACCACTTTAACTGTCATTGCTAAAGGAAATGGTGGTGTTGATGAATTGGTCGGCCATT
TGAATTCAAAGATCGTTGCTTATGGCTTAGTTCGTGAAGTTGAAAGATTCGATCTCTCTG
ATACCATCAAATTTGCTTTCATCGATTTCGTTGGTGAAGATATCAATCGTATGTTTAGAG
CTAAACTTGGTACTCATTCTGGTACTGTTAAACAATTATTCCATCCATATCATGTTGATT
NACATGTCTCCTCTCCTTCTNAANTATCCTCNAAATCGTTACTAAAACTATCACTCAAAA
TTACGGNACTCCTCAAGA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---RARLGTHTGFVNQLVTPYHVDIKCTVKSEISKEIVEEAVAKASGKASMVLSDDAQSR
GSGGRMTSSYSSSSASTQGSNTTGKTFAGAGSKFTTPSAVPKSSGSTLGFDDEQQVRDTI
KDVRSDSTDTNWCLVGYKNDTTLTVIAKGNGGVDELVGHLNSKIVAYGLVREVERFDLSD
TIKFAFIDFVGEDINRMFRAKLGTHSGTVKQLFHPYHVDXHVSSPSXXSSKSLLKLSLKI
TXLLK
Translated Amino Acid sequence (All Frames) 
Frame A:
---RARLGTHTGFVNQLVTPYHVDIKCTVKSEISKEIVEEAVAKASGKASMVLSDDAQSR
GSGGRMTSSYSSSSASTQGSNTTGKTFAGAGSKFTTPSAVPKSSGSTLGFDDEQQVRDTI
KDVRSDSTDTNWCLVGYKNDTTLTVIAKGNGGVDELVGHLNSKIVAYGLVREVERFDLSD
TIKFAFIDFVGEDINRMFRAKLGTHSGTVKQLFHPYHVDXHVSSPSXXSSKSLLKLSLKI
TXLLK


Frame B:
---ehv*vlildl*is*lhhimlilnvqlnqkfqkrl*rkplqkhqvkhqwy*vmmlnhv
vvvvv*pqvtvhqvlqlkvqtqpvkhllvlvvnlqhhqlcqnqvvqhlvsmmnnkleiqs
kmldpivltligv*lvikmipl*lsllkemvvlmnwsai*iqrsllma*fvklkdsisli
psnllssislvkisivclelnlvlilvllnnysihimlixmsplllxypxnry*nyhskl
rxssr


Frame C:
---stfrysywicksvsytisc*y*mys*irnfkrdcrgsrcksir*singie**csitw
*wwsydlklqfikcfnsrfkhnr*nicwcw**iyntiscakikwfntwfr**tts*rynq
rc*ir*y*h*lvfswl*k*yhfnchc*rkwwc**igrpfefkdrclwlss*s*kirsl*y
hqicfhrfrw*ryqsyv*s*twysfwyc*tiipsisc*xtcllsfxxilxivtktitqny
gtpq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH247 (AFH247Q) /CSM/AF/AFH2-B/AFH247Q.Seq.d/                       1413   0.0
SFA542 (SFA542Q) /CSM/SF/SFA5-B/SFA542Q.Seq.d/                       1392   0.0
AFL615 (AFL615Q) /CSM/AF/AFL6-A/AFL615Q.Seq.d/                       1382   0.0
VHH653 (VHH653Q) /CSM/VH/VHH6-C/VHH653Q.Seq.d/                       1380   0.0
VFI640 (VFI640Q) /CSM/VF/VFI6-B/VFI640Q.Seq.d/                       1380   0.0
SFI143 (SFI143Q) /CSM/SF/SFI1-B/SFI143Q.Seq.d/                       1380   0.0
SFF593 (SFF593Q) /CSM/SF/SFF5-D/SFF593Q.Seq.d/                       1380   0.0
SFE348 (SFE348Q) /CSM/SF/SFE3-B/SFE348Q.Seq.d/                       1380   0.0
CHB476 (CHB476Q) /CSM/CH/CHB4-D/CHB476Q.Seq.d/                       1380   0.0
CFC366 (CFC366Q) /CSM/CF/CFC3-C/CFC366Q.Seq.d/                       1380   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116922|AC116922.2 Dictyostelium discoideum chromosome 2 map 6061442-6097630 strain AX4, complete sequence.  1324   0.0    2
AQ651762|AQ651762.1 Sheared DNA-20H15.TR Sheared DNA Trypanosoma brucei genomic clone Sheared DNA-20H15, DNA sequence.    50   0.046  1
X58243|X58243.1 C.ruminantium (clone pCR9) DNA for unknown ORF.    36   0.50   3
CC312353|CC312353.1 TAM32-15K10_Sp6.1 TAM32 Gallus gallus genomic clone TAM32-15K10, DNA sequence.    30   1.1    4
CE586668|CE586668.1 tigr-gss-dog-17000366347460 Dog Library Canis familiaris genomic, genomic survey sequence.    38   1.3    2
CL251802|CL251802.1 ZMMBBb0604N14f ZMMBBb (HindIII) Zea mays subsp. mays genomic clone ZMMBBb0604N14 5', genomic survey sequence.    44   2.8    1
CF192909|CF192909.1 l5g03j2.r1 Cryptococcus neoformans strain B3501 Cryptococcus neoformans var. neoformans cDNA clone l5g03j2 5', mRNA sequence.    44   2.8    1
AL132841|AL132841.1 Caenorhabditis elegans YAC Y15E3A.             38   6.8    4
CQ330218|CQ330218.1 Sequence 4312 from Patent WO0157275.           30   9.0    3
CQ293263|CQ293263.1 Sequence 4368 from Patent WO0186003.           30   9.0    3
dna update 2004. 4. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116922_4(AC116922|pid:none) Dictyostelium discoideum chromosom...   373   e-103
AY122215_1(AY122215|pid:none) Drosophila melanogaster LP08941 fu...    54   4e-06
AE017342_92(AE017342|pid:none) Cryptococcus neoformans var. neof...    51   3e-05
BC095818_1(BC095818|pid:none) Danio rerio zgc:112450, mRNA (cDNA...    49   2e-04
AK153389_1(AK153389|pid:none) Mus musculus bone marrow macrophag...    48   3e-04
DQ443285_1(DQ443285|pid:none) Bombyx mori cyclic AMP-regulated p...    48   3e-04
AB038364_1(AB038364|pid:none) Rattus norvegicus mRNA for SH3P7r4...    47   4e-04
AB039818_1(AB039818|pid:none) Rattus norvegicus mRNA for SH3P7r2...    47   4e-04
(Q9JHL4) RecName: Full=Drebrin-like protein; AltName: Full=SH3 d...    47   4e-04
(Q80WP1) RecName: Full=Drebrin-like protein; AltName: Full=SH3 d...    47   4e-04
protein update 2009. 4.21
PSORT 

 psg: 0.86  gvh: 0.47  alm: 0.48  top: 0.30  tms: 0.00  mit: 0.33  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 20.0 %: extracellular, including cell wall
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFH247 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFH247Z
3' end seq. 
>AFH247Z.Seq
----------AAGAGCACGTTTAGGTACTCATACTGGATTTGTAAATCAGTTAGTTACAC
CATATCATGTTGATATTAAATGTACAGTTAAATCAGAAATTTCAAAAGAGATTGTAGAGG
AAGCCGTTGCAAAAGCATCAGGTAAAGCATCAATGGTATTGAGTGATGATGCTCAATCAC
GTGGTAGTGGTGGTCGTATGACCTCAAGTTACAGTTCATCAAGTGCTTCAACTCAAGGTT
CAAACACAACCGGTAAAACATTTGCTGGTGCTGGTAGTAAATTTACAACACCATCAGCTG
TGCCAAAATCAAGTGGTTCAACACTTGGTTTCGATGATGAACAACAAGTTAGAGATACAA
TCAAAGATGTTAGATCCGATAGTACTGACACTAATTGGTGTTTAGTTGGTTATAAAAATG
ATACCACTTTAACTGTCATTGCTAAAGGAAATGGTGGTGTTGATGAATTGGTCGGCCATT
TGAATTCAAAGATCGTTGCTTATGGCTTAGTTCGTGAAGTTGAAAGATTCGATCTCTCTG
ATACCATCAAATTTGCTTTCATCGATTTCGTTGGTGAAGATATCAATCGTATGTTTAGAG
CTAAACTTGGTACTCATTCTGGTACTGTTAAACAATTATTCCATCCATATCATGTTGATT
NACATGTCTCCTCTCCTTCTNAANTATCCTCNAAATCGTTACTAAAACTATCACTCAAAA
TTACGGNACTCCTCAAGA
Length of 3' end seq. 728
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -