>AFH245 (AFH245Q) /CSM/AF/AFH2-B/AFH245Q.Seq.d/
CAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAACATCCT
AACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAATTATT
AAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTTGGTGG
TAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAATAAAGT
ATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAATTCCAT
GGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTCCTTAA
AGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATGCTTGG
TCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGATTTCCT
CCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAATTCTGC
AGGCTGTTGGAACATTTTAACCTATCAACCATTAXXXXXXXXXXGTTCAGCTTGTTCACG
TGCTTACATTCCACAATCTTTATGGCCCCAAATTAAAGATAGATTAGTTACTGGTGTTAA
ATCAATGAAAATGGGTCAATCTGATGATTTCTCTTCATTCGTTTCAGCTGTCATCGATAA
AAACTCTTTCAACAATATTCAATCATACATTGAACATGCTAAAGCTTCACCAGATGCTGA
AATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACTATCAT
CCTTGCCAAAGATCCACATTACAAATCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAAC
CATTTATGTTTATGAAGATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAAACTTC
ACCATATGCTTTAACTGGTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACCGCTCA
CAAATACTTGAAGAATGCTGCTGGTAACTTTTATATCAATGATAAATGCACTGGTGCCGT
CGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTC
ATCATTAAATCTTTTAAGATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTCCCACT
CACTAGTTTCACTTATCCATACATGATGATCCAGAAAACTAAATAATTTNAACTAAATAA
ATACAAAANA

kh*KNTSRVLMSHFARANILTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCVVGG
KEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAVFLK
AADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPANSA
GCWNILTYQPL---

---SACSRAYIPQSLWPQIKDRLVTGVKSMKMGQSDDFSSFVSAVIDKNSFNNIQSYIEH
AKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVLTIYVYEDSKFEET
LKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGAVVGQQPFGGSRAS
GTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYMMIQKTK*fxlnkykx

Frame A:
qtlkkhiksthvslcks*hpntg*ptnvkl*swfnt*kiikrsmc*i*khnnrysmccww
*rnsyw*cskainll*s**sighlpssqcritqisr*kfhgi*spmgiiti*skkcsfp*
scrfvkyqvpl*clsfyyawsr*nclasrn*cssrry*fppfqc*irsrnlpttttsqfc
rllehfnlsti---

---vqlvhvltfhnlygpklkid*llvlnq*kwvnlmislhsfqlssiktlstifnhtln
mlklhqmlkslsvvnviallvgllnqlsslpkihitnqwkkkfsvqs*pfmfmkilnlkk
h*ksvmklhhml*lvqsfqlvdmllkpltnt*rmllvtfisminalvpslvnnhlvvlvl
qvqtiklvhh*if*dgsapeqskktlshslvsliht**srklnnxn*intk

Frame B:
kh*KNTSRVLMSHFARANILTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCVVGG
KEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAVFLK
AADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPANSA
GCWNILTYQPL---

---fslftclhstifmapn*r*isywc*inengsi**flfirfschr*klfqqysiih*t
c*sftrc*nhcrw*m**lcwlvc*tnyhpcqrstlqingrrnfrsslnhlcl*rf*i*rn
iknl**nfticfnwfnlfnl*icy*nrsqileeccw*llyq**mhwcrrwsttiwwfscf
rykr*swfiiksfkmdqrqnnqrklcpth*fhlsihddpen*iixtk*iqx

Frame C:
nikkthqeyscltlqelts*hrltnqc*imklvqhieny*kkhvlnletqqsifhvllvv
kkfvlvmfksn*faliiikywppsikpmpnysn*plkipwnlkpngnhyhlkqevqfslk
lqic*ipstvmms*lllclvkvklfgkqklmqqqkvlissvsmlntlkkftnnnhqpilq
avgtf*pinh---

---SACSRAYIPQSLWPQIKDRLVTGVKSMKMGQSDDFSSFVSAVIDKNSFNNIQSYIEH
AKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVLTIYVYEDSKFEET
LKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGAVVGQQPFGGSRAS
GTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYMMIQKTK*fxlnkykx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH245 (AFH245Q) /CSM/AF/AFH2-B/AFH245Q.Seq.d/                       2418   0.0
AFM489 (AFM489Q) /CSM/AF/AFM4-D/AFM489Q.Seq.d/                       1342   0.0
VFG887 (VFG887Q) /CSM/VF/VFG8-D/VFG887Q.Seq.d/                       1336   0.0
VFE114 (VFE114Q) /CSM/VF/VFE1-A/VFE114Q.Seq.d/                       1336   0.0
CFI826 (CFI826Q) /CSM/CF/CFI8-B/CFI826Q.Seq.d/                       1336   0.0
CFF617 (CFF617Q) /CSM/CF/CFF6-A/CFF617Q.Seq.d/                       1336   0.0
AFG621 (AFG621Q) /CSM/AF/AFG6-A/AFG621Q.Seq.d/                       1336   0.0
AFE247 (AFE247Q) /CSM/AF/AFE2-B/AFE247Q.Seq.d/                       1336   0.0
SFK660 (SFK660Q) /CSM/SF/SFK6-C/SFK660Q.Seq.d/                       1334   0.0
AFD340 (AFD340Q) /CSM/AF/AFD3-B/AFD340Q.Seq.d/                       1334   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL031786|AL031786.1 S.pombe chromosome II cosmid c24C6.            88   1e-17  2
D89230|D89230.1 Schizosaccharomyces pombe mRNA, partial cds, clone: SY 1373.    80   9e-11  1
CF192429|CF192429.1 l4g02j2.f1 Cryptococcus neoformans strain B3501 Cryptococcus neoformans var. neoformans cDNA clone l4g02j2 3', mRNA sequence.    70   9e-08  1
AL416406|AL416406.1 T3 end of clone AX0AA014C08 of library AX0AA from strain CBS 7064 of Pichia farinosa.    56   3e-05  2
AC090078|AC090078.2 Trypanosoma cruzi clone GAMFS45 strain CL Brener, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    60   8e-05  1
AL393468|AL393468.1 T3 end of clone AR0AA011B08 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    60   8e-05  1
AL416312|AL416312.1 T7 end of clone AX0AA013D07 of library AX0AA from strain CBS 7064 of Pichia farinosa.    48   1e-04  2
AZ932439|AZ932439.1 474.dhz99a06.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz99a06.s1, DNA sequence.    58   3e-04  1
BH391341|BH391341.1 AG-ND-107E4.TF ND-TAM Anopheles gambiae genomic clone AG-ND-107E4, DNA sequence.    56   0.001  1
AX620724|AX620724.1 Sequence 3687 from Patent WO02094868.          36   0.002  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RA2) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   436   e-120
EF157670_5(EF157670|pid:none) Uncultured bacterium CBNPD1 BAC cl...   291   4e-77
CP001087_1672(CP001087|pid:none) Desulfobacterium autotrophicum ...   286   1e-75
CP000140_2188(CP000140|pid:none) Parabacteroides distasonis ATCC...   284   4e-75
CU466930_1098(CU466930|pid:none) Candidatus Cloacamonas acidamin...   268   3e-70
AP009380_1401(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...   268   3e-70
CU207366_389(CU207366|pid:none) Gramella forsetii KT0803 complet...   267   6e-70
CP000251_828(CP000251|pid:none) Anaeromyxobacter dehalogenans 2C...   266   8e-70
CP001359_872(CP001359|pid:none) Anaeromyxobacter dehalogenans 2C...   266   1e-69
CP001034_1725(CP001034|pid:none) Natranaerobius thermophilus JW/...   265   2e-69

 psg: 0.75  gvh: 0.46  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for AFH245 is mit

>AFH245F.Seq
CAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAACATCCT
AACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAATTATT
AAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTTGGTGG
TAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAATAAAGT
ATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAATTCCAT
GGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTCCTTAA
AGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATGCTTGG
TCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGATTTCCT
CCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAATTCTGC
AGGCTGTTGGAACATTTTAACCTATCAACCATTA----------

>AFH245Z.Seq
----------GTTCAGCTTGTTCACGTGCTTACATTCCACAATCTTTATGGCCCCAAATT
AAAGATAGATTAGTTACTGGTGTTAAATCAATGAAAATGGGTCAATCTGATGATTTCTCT
TCATTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCATACATTGAA
CATGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTT
GGTTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAATCAATGGAA
GAAGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAA
ACATTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACT
TGTAGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTAT
ATCAATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCT
TCAGGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGA
ACAATCAAAGAAAACTTTGTCCCACTCACTAGTTTCACTTATCCATACATGATGATCCAG
AAAACTAAATAATTTNAACTAAATAAATACAAAANA

>AFH245P.Seq
CAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAACATCCT
AACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAATTATT
AAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTTGGTGG
TAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAATAAAGT
ATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAATTCCAT
GGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTCCTTAA
AGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATGCTTGG
TCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGATTTCCT
CCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAATTCTGC
AGGCTGTTGGAACATTTTAACCTATCAACCATTA----------GTTCAGCTTGTTCACG
TGCTTACATTCCACAATCTTTATGGCCCCAAATTAAAGATAGATTAGTTACTGGTGTTAA
ATCAATGAAAATGGGTCAATCTGATGATTTCTCTTCATTCGTTTCAGCTGTCATCGATAA
AAACTCTTTCAACAATATTCAATCATACATTGAACATGCTAAAGCTTCACCAGATGCTGA
AATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACTATCAT
CCTTGCCAAAGATCCACATTACAAATCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAAC
CATTTATGTTTATGAAGATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAAACTTC
ACCATATGCTTTAACTGGTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACCGCTCA
CAAATACTTGAAGAATGCTGCTGGTAACTTTTATATCAATGATAAATGCACTGGTGCCGT
CGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTC
ATCATTAAATCTTTTAAGATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTCCCACT
CACTAGTTTCACTTATCCATACATGATGATCCAGAAAACTAAATAATTTNAACTAAATAA
ATACAAAANA