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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHP591
Library CH
(Link to library)
Clone ID CHP591
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID CHP591P
(Link to Original site)
Representative DNA sequence 
>CHP591 (CHP591Q) /CSM/CH/CHP5-D/CHP591Q.Seq.d/
ACTGTTGGCCTACTGGNACTTTTTTATTTTTTGTTAAAGTACAAAATGGTTAATTTCACA
ATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATT
GCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATT
ATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAG
CGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGAC
AAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGT
CACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTC
GTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTC
GCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTC
CAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTC
ATTGTTGGTAACACTGAAGXXXXXXXXXXCCAATGGGCCACCAAAGAAGGGTGTTGTTTT
GTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATG
CCATCCACANAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGCTG
AATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAG
AAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTG
AAGAACGTCGTATCGGTTCCCCNTTATTNTCTGTCAAAGCTCATTTACCAGTATTANAAA
CNNTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTG
TNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCG
CTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATTCCCAGAT
sequence update 2002.10.25
Translated Amino Acid sequence 
TVGLLXLFYFLLKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGI
IADKVSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPG
HVDFSSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLEL
QLNTEEAYLSFRRAIESVNVIVGNTE---

---PMGHQRRVLFCDENMRGIRFNLYDVTLHTDAIHXGGGQIIPTARRVLYAAELTASPT
XLEPXYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLXSVKAHLPVLXTXGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRXRKGLAPEIPR
Translated Amino Acid sequence (All Frames) 
Frame A:
TVGLLXLFYFLLKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGI
IADKVSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPG
HVDFSSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLEL
QLNTEEAYLSFRRAIESVNVIVGNTE---

---PMGHQRRVLFCDENMRGIRFNLYDVTLHTDAIHXGGGQIIPTARRVLYAAELTASPT
XLEPXYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLXSVKAHLPVLXTXGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRXRKGLAPEIPR

Frame B:
llaywxffifc*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvl
lpikflvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqv
tlisvqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*ns
n*tlkklislsvellnpsmsllvtlk---

---qwatkegccfvmkt*evsvsifmmspstlmpstxvvvkssqllvvyxmlln*lppql
x*nqxt**kllpqktllvvftlysivvevlllvknvvsvpxyxlskliyqyxkxsvspli
yvptlpvklshnvxsiiglqlvlstkirkplksl*ppexekv*pqkfpd

Frame C:
cwptgtflffvkvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyy
cr*sfw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprs
r*fqfrsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtp
ikh*rslslfp*sy*irqchcw*h*---

---ngppkkgvvl**kherypfqsl*chpph*chpxrwwsnhpncssctxcc*inclpnx
xrtnxlsrnycprkrywwyllctqss*rycyw*rtsyrfpxixcqssftsixnxrfhr*f
tfphcrssfptmcxrslgfnwccqqr*esh*srfshqkxkrfsprnsq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHP591 (CHP591Q) /CSM/CH/CHP5-D/CHP591Q.Seq.d/                       2042   0.0
CHS236 (CHS236Q) /CSM/CH/CHS2-B/CHS236Q.Seq.d/                       1164   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1160   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1160   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1160   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1160   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1160   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1160   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1160   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1160   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1162   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    66   2e-51  10
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   4e-48  7
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   7e-48  7
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    62   2e-42  7
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   2e-33  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   3e-33  4
CX154971|CX154971.1 TfEST089A07 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   7e-33  4
CX154356|CX154356.1 TfEST029E10 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   1e-32  4
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    62   1e-31  5
dna update 2005.10. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   2e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   256   7e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   7e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   1e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   1e-66
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   254   5e-66
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   253   8e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   253   8e-66
protein update 2009. 4.11
PSORT 

 psg: 0.94  gvh: 0.35  alm: 0.34  top: 0.60  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for CHP591 is cyt
5' end seq. ID CHP591F
5' end seq. 
>CHP591F.Seq
ACTGTTGGCCTACTGGNACTTTTTTATTTTTTGTTAAAGTACAAAATGGTTAATTTCACA
ATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATT
GCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATT
ATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAG
CGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGAC
AAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGT
CACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTC
GTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTC
GCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTC
CAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTC
ATTGTTGGTAACACTGAAGNNNNNNNNNN
Length of 5' end seq. 629
3' end seq. ID CHP591Z
3' end seq. 
>CHP591Z.Seq
NNNNNNNNNNCCAATGGGCCACCAAAGAAGGGTGTTGTTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACANAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGCTGAATTAACTGCCTCCCCAAC
TNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTAT
TTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTC
CCCNTTATTNTCTGTCAAAGCTCATTTACCAGTATTANAAACNNTCGGTTTCACCGCTGA
TTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTTC
AATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAG
AAAAGGTTTAGCCCCAGAAATTCCCAGAT
Length of 3' end seq. 509
Connected seq. ID CHP591P
Connected seq. 
>CHP591P.Seq
ACTGTTGGCCTACTGGNACTTTTTTATTTTTTGTTAAAGTACAAAATGGTTAATTTCACA
ATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATT
GCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATT
ATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAG
CGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGAC
AAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGT
CACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTC
GTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTC
GCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTC
CAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTC
ATTGTTGGTAACACTGAAG----------CCAATGGGCCACCAAAGAAGGGTGTTGTTTT
GTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATG
CCATCCACANAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGCTG
AATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAG
AAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTG
AAGAACGTCGTATCGGTTCCCCNTTATTNTCTGTCAAAGCTCATTTACCAGTATTANAAA
CNNTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTG
TNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCG
CTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATTCCCAGAT
Length of connected seq. 1118
Full length Seq ID -
Full length Seq. -
Length of full length seq. -