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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH226
Library AF
(Link to library)
Clone ID AFH226
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFH226P
(Link to Original site)
Representative DNA sequence 
>AFH226 (AFH226Q) /CSM/AF/AFH2-B/AFH226Q.Seq.d/
ACTGTTGGCCTACTGGNATTTTTTCTCACCATATTTTTTTGTAAATAGTAATAATTAATA
ATAATTTTTTAAATTCTTTATTTTTTTAAACATATAAAAATTTTTTTTTTCCTTNGNATA
TTATTTTTTTTTCCATTTTTACATATTAATTAATTATGCAAATTTTNGTTAAAACTCTCA
CTGGNAAAACCATCACATNGGAAGTNGAAGGTAGNGACANCATTGAAAATGTCANGGCTA
AAATTCAAGACAAAGAAGGTNTXXXXXXXXXXCCCCTTGAAAGTCGNAAGGGNAGNGAAA
ACCTTTTAAAAATGNCAAAGCCCAAAAATNCAAGGCAAAGAAGGTNTTCCCCCAANATAA
ANAAGGNNTNATTTTTGNCNGGTAAACAATTAAAAGANGGCCGNACTNTCTCTGATTTCA
ACATCCAAAAAGAATCCCCTNTCCATTTAGTTNTCANANTAANAGGTGGTANGCAAATNT
TTGTAAAAACCTTNNCTGGTAANACCNTCNCATTGGAAGTNGAAGGTTNTGANAACNTTG
AAAATGTCAAAGCCNAAATTCAAGACAAANAAGGNATTCCCCCNGATCAACAAANATTAA
TTTTNGCTGGNAAACAATTAAAANATGGTNGNACTCTCTNTGACTACAANATTCAAAAGG
AATCCCCTCTCCNTTTAGTTNTCANATTAAAAGGTGGNANGCAAATNTTTGTTAAAACNT
TAACAGGNAAAACNATCCCCNTGGAAGTCGAAGGTAGTGANAACNTTGAAAATGTTAAAG
CCNAAANCCNANACAAANAAGGNATTCCCCCNGANCAACAACGNNTCATTTTCGCTGGNA
AACNATTANAANAAGGTCGGACCCCCCCNGANTACAACNTTCNAAAGGAANCCCCCCCCC
CTTTAGNTCCCAGATTAAAAGGNGGNA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
cwptgifshhifl*iviinnnflnslff*tyknfffpxxiiffsifty*LIMQIXVKTLT
GKTITXEVEGXDXIENVXAKIQDKEG---

---PLESRKGXENLLKMXKPKNXRQRRXSPXIXKXXFLXGKQLKXGRTXSDFNIQKESPX
HLVXXXXGGXQXFVKTXXGXTXXLEVEGXXNXENVKAXIQDKXGIPPDQQXLIXAGKQLK
XGXTLXDYXIQKESPLXLVXXLKGGXQXFVKTLTGKTIPXEVEGSXNXENVKAXXXXKXG
IPPXQQRXIFAGKXLXXGRTPPXYNXXKEXPPPLXPRLKGG
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxffltiffck***liiif*ilyffkhikiffflxilfffpflhin*lckfxlkls
lxkpshxkxkvxtxlkmsxlkfktkkv---

---PLESRKGXENLLKMXKPKNXRQRRXSPXIXKXXFLXGKQLKXGRTXSDFNIQKESPX
HLVXXXXGGXQXFVKTXXGXTXXLEVEGXXNXENVKAXIQDKXGIPPDQQXLIXAGKQLK
XGXTLXDYXIQKESPLXLVXXLKGGXQXFVKTLTGKTIPXEVEGSXNXENVKAXXXXKXG
IPPXQQRXIFAGKXLXXGRTPPXYNXXKEXPPPLXPRLKGG

Frame B:
llaywxffspyffvnsnn***ffkffiflni*kfffsxxyyfffhfyilinyanfx*nsh
wxnhhxgsxr*xxh*kcxg*nsrqrrx---

---plkvxrxxktf*kxqspkxqgkegxppx*xrxxfxxvnn*kxaxlslistskknpls
i*xsx*xvvxkxl*kpxlvxpshwkxkvxxtlkmskpkfktxkxfpxinkx*fxlxnn*x
mvxlsxttxfkrnplsx*xsx*kvxxkxllkx*qxkxspwkskvvxtlkmlkpkxxtxkx
fpxxnnxsfslxnxxxkvgppxxttfxrxpppl*xpd*kxx

Frame C:
cwptgifshhifl*iviinnnflnslff*tyknfffpxxiiffsifty*LIMQIXVKTLT
GKTITXEVEGXDXIENVXAKIQDKEG---

---p*ksxgxxkpfknxkaqkxkakkvfpqxkxgxifxx*tikrxpxxl*fqhpkripxp
fsxxxxrwxanxcknlxw*xxxigsxrx*xx*kcqsxnsrqxrxspxstxinfxwxtikx
wxxsl*lqxskgipspfsxxikrwxanxc*nxnrxnxpxgsrr**xx*kc*sxxpxqxrx
spxxttxhfrwxtixxrsdppxxqxskgxpppfxsqikrx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH226 (AFH226Q) /CSM/AF/AFH2-B/AFH226Q.Seq.d/                        906   0.0
CFG807 (CFG807Q) /CSM/CF/CFG8-A/CFG807Q.Seq.d/                        559   e-158
SFA506 (SFA506Q) /CSM/SF/SFA5-A/SFA506Q.Seq.d/                        543   e-153
CFD217 (CFD217Q) /CSM/CF/CFD2-A/CFD217Q.Seq.d/                        543   e-153
AFE384 (AFE384Q) /CSM/AF/AFE3-D/AFE384Q.Seq.d/                        539   e-152
VFF893 (VFF893Q) /CSM/VF/VFF8-D/VFF893Q.Seq.d/                        531   e-150
SLC239 (SLC239Q) /CSM/SL/SLC2-B/SLC239Q.Seq.d/                        531   e-150
SFJ530 (SFJ530Q) /CSM/SF/SFJ5-B/SFJ530Q.Seq.d/                        531   e-150
CFK385 (CFK385Q) /CSM/CF/CFK3-D/CFK385Q.Seq.d/                        531   e-150
CFG825 (CFG825Q) /CSM/CF/CFG8-B/CFG825Q.Seq.d/                        531   e-150
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   531   0.0    3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   141   8e-80  5
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   141   2e-79  5
M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   139   7e-79  5
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   214   1e-72  4
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   230   4e-71  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   230   8e-71  3
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   157   2e-55  4
CB887059|CB887059.1 Lice_02_J02_T3 Body louse cDNA library Pediculus humanus cDNA, mRNA sequence.   113   2e-39  3
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.    92   4e-39  4
dna update 2004. 3.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   256   5e-67
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   256   5e-67
A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   253   5e-66
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   253   5e-66
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   253   5e-66
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   245   2e-63
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   245   2e-63
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   245   2e-63
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   245   2e-63
AK056939_1(AK056939|pid:none) Homo sapiens cDNA FLJ32377 fis, cl...   244   3e-63
protein update 2009. 4.21
PSORT 

 psg: 0.78  gvh: 0.37  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for AFH226 is nuc
5' end seq. ID AFH226F
5' end seq. 
>AFH226F.Seq
ACTGTTGGCCTACTGGNATTTTTTCTCACCATATTTTTTTGTAAATAGTAATAATTAATA
ATAATTTTTTAAATTCTTTATTTTTTTAAACATATAAAAATTTTTTTTTTCCTTNGNATA
TTATTTTTTTTTCCATTTTTACATATTAATTAATTATGCAAATTTTNGTTAAAACTCTCA
CTGGNAAAACCATCACATNGGAAGTNGAAGGTAGNGACANCATTGAAAATGTCANGGCTA
AAATTCAAGACAAAGAAGGTNT----------
Length of 5' end seq. 262
3' end seq. ID AFH226Z
3' end seq. 
>AFH226Z.Seq
----------CCCCTTGAAAGTCGNAAGGGNAGNGAAAACCTTTTAAAAATGNCAAAGCC
CAAAAATNCAAGGCAAAGAAGGTNTTCCCCCAANATAAANAAGGNNTNATTTTTGNCNGG
TAAACAATTAAAAGANGGCCGNACTNTCTCTGATTTCAACATCCAAAAAGAATCCCCTNT
CCATTTAGTTNTCANANTAANAGGTGGTANGCAAATNTTTGTAAAAACCTTNNCTGGTAA
NACCNTCNCATTGGAAGTNGAAGGTTNTGANAACNTTGAAAATGTCAAAGCCNAAATTCA
AGACAAANAAGGNATTCCCCCNGATCAACAAANATTAATTTTNGCTGGNAAACAATTAAA
ANATGGTNGNACTCTCTNTGACTACAANATTCAAAAGGAATCCCCTCTCCNTTTAGTTNT
CANATTAAAAGGTGGNANGCAAATNTTTGTTAAAACNTTAACAGGNAAAACNATCCCCNT
GGAAGTCGAAGGTAGTGANAACNTTGAAAATGTTAAAGCCNAAANCCNANACAAANAAGG
NATTCCCCCNGANCAACAACGNNTCATTTTCGCTGGNAAACNATTANAANAAGGTCGGAC
CCCCCCNGANTACAACNTTCNAAAGGAANCCCCCCCCCCTTTAGNTCCCAGATTAAAAGG
NGGNA
Length of 3' end seq. 655
Connected seq. ID AFH226P
Connected seq. 
>AFH226P.Seq
ACTGTTGGCCTACTGGNATTTTTTCTCACCATATTTTTTTGTAAATAGTAATAATTAATA
ATAATTTTTTAAATTCTTTATTTTTTTAAACATATAAAAATTTTTTTTTTCCTTNGNATA
TTATTTTTTTTTCCATTTTTACATATTAATTAATTATGCAAATTTTNGTTAAAACTCTCA
CTGGNAAAACCATCACATNGGAAGTNGAAGGTAGNGACANCATTGAAAATGTCANGGCTA
AAATTCAAGACAAAGAAGGTNT----------CCCCTTGAAAGTCGNAAGGGNAGNGAAA
ACCTTTTAAAAATGNCAAAGCCCAAAAATNCAAGGCAAAGAAGGTNTTCCCCCAANATAA
ANAAGGNNTNATTTTTGNCNGGTAAACAATTAAAAGANGGCCGNACTNTCTCTGATTTCA
ACATCCAAAAAGAATCCCCTNTCCATTTAGTTNTCANANTAANAGGTGGTANGCAAATNT
TTGTAAAAACCTTNNCTGGTAANACCNTCNCATTGGAAGTNGAAGGTTNTGANAACNTTG
AAAATGTCAAAGCCNAAATTCAAGACAAANAAGGNATTCCCCCNGATCAACAAANATTAA
TTTTNGCTGGNAAACAATTAAAANATGGTNGNACTCTCTNTGACTACAANATTCAAAAGG
AATCCCCTCTCCNTTTAGTTNTCANATTAAAAGGTGGNANGCAAATNTTTGTTAAAACNT
TAACAGGNAAAACNATCCCCNTGGAAGTCGAAGGTAGTGANAACNTTGAAAATGTTAAAG
CCNAAANCCNANACAAANAAGGNATTCCCCCNGANCAACAACGNNTCATTTTCGCTGGNA
AACNATTANAANAAGGTCGGACCCCCCCNGANTACAACNTTCNAAAGGAANCCCCCCCCC
CTTTAGNTCCCAGATTAAAAGGNGGNA
Length of connected seq. 917
Full length Seq ID -
Full length Seq. -
Length of full length seq. -