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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH224
Library AF
(Link to library)
Clone ID AFH224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFH224Z
(Link to Original site)
Representative DNA sequence 
>AFH224 (AFH224Q) /CSM/AF/AFH2-A/AFH224Q.Seq.d/
XXXXXXXXXXCCATTATATGGTTTAGGGGANATTACCACAAGCATTTGCCCGTTGATCTG
CTATCTATGGGTGGNACATATATGCTTNATAAACCANATTGAAAATATTGTTTTCGATGA
AAAAAANAAAATTATTAAAGTTACATCTGAAGGTNAAACTGTAACATGTGATAAAATTGT
TTGTGATCCATCATATTTCCCAAATAAAGTTCAAAAGAAAGGTAAAATTATTCGTTCAAT
TTGTATTTTGAATGGTCCAATTCCATCTGCTGATAAGAAATTTGAATCACTCCAAATCAT
TATCCCACAAAAACAAGTTAAACGTCAATCAGATATGTACGTTGGTGTTATGTCATCCGC
TCATTGTGTCTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCACTGTTGAAACCAA
TGAACCAGAGAAAGAATTAGCACCAGCTTACGCTTTATTAGGTCCAATTCAAGAGAAATT
TGTCTATATTTCTGATTTCATTGAACCAATTGCTGATGGTACTGCCGACAATGTTTTCAT
CTCAAAATCATATGATGCTACCTCTCACTTTGAAACCACTTGTGAAGATGTCATGGATAT
GTACAAGAGAATCACNGGTAAAGATT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---LYGLGXITTSICPLICYLWVXHICXINXIENIVFDEKXKIIKVTSEGXTVTCDKIVC
DPSYFPNKVQKKGKIIRSICILNGPIPSADKKFESLQIIIPQKQVKRQSDMYVGVMSSAH
CVCPKGKYIAIVSATVETNEPEKELAPAYALLGPIQEKFVYISDFIEPIADGTADNVFIS
KSYDATSHFETTCEDVMDMYKRITGKD
Translated Amino Acid sequence (All Frames) 
Frame A:
---hymv*gxlpqafar*saiygwxiyax*txlkilfsmkkxkllklhlkvkl*hviklf
vihhisqikfkrkvklfvqfvf*mvqfhllirnlnhskslshknklnvnqictlvlchpl
ivsvqrvntlpsfqpllkpmnqrkn*hqltly*vqfkrnlsiflislnqllmvlptmfss
qnhmmlpltlkplvkmswictresxvki


Frame B:
---iiwfrgxyhkhlpvdllsmggtymlxkpx*kycfr*kkxny*syi*rxncnm**ncl
*siifpk*ssker*nysfnlyfewsnsic**ei*itpnhyptkts*tsiryvrwcyvirs
lclskg*ihchrfshc*nq*treristslrfirsnsreiclyf*fh*tnc*wycrqcfhl
kii*cylsl*nhl*rchgyvqenhx*r


Frame C:
---LYGLGXITTSICPLICYLWVXHICXINXIENIVFDEKXKIIKVTSEGXTVTCDKIVC
DPSYFPNKVQKKGKIIRSICILNGPIPSADKKFESLQIIIPQKQVKRQSDMYVGVMSSAH
CVCPKGKYIAIVSATVETNEPEKELAPAYALLGPIQEKFVYISDFIEPIADGTADNVFIS
KSYDATSHFETTCEDVMDMYKRITGKD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH224 (AFH224Q) /CSM/AF/AFH2-A/AFH224Q.Seq.d/                       1138   0.0
SFL394 (SFL394Q) /CSM/SF/SFL3-D/SFL394Q.Seq.d/                       1086   0.0
SFJ795 (SFJ795Q) /CSM/SF/SFJ7-D/SFJ795Q.Seq.d/                       1086   0.0
SFJ449 (SFJ449Q) /CSM/SF/SFJ4-C/SFJ449Q.Seq.d/                       1086   0.0
SFI510 (SFI510Q) /CSM/SF/SFI5-A/SFI510Q.Seq.d/                       1086   0.0
SFE141 (SFE141Q) /CSM/SF/SFE1-B/SFE141Q.Seq.d/                       1086   0.0
SFD219 (SFD219Q) /CSM/SF/SFD2-A/SFD219Q.Seq.d/                       1086   0.0
CHD258 (CHD258Q) /CSM/CH/CHD2-C/CHD258Q.Seq.d/                       1086   0.0
CHB577 (CHB577Q) /CSM/CH/CHB5-D/CHB577Q.Seq.d/                       1086   0.0
CFC760 (CFC760Q) /CSM/CF/CFC7-C/CFC760Q.Seq.d/                       1086   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI315670|BI315670.1 saf75f12.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONEINHIBITOR. ;, mRNA sequence.    56   5e-06  2
AI437629|AI437629.1 sa37e09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-1505 5' similar to TR:O24653 O24653 GDI2. ;, mRNA sequence.    56   5e-06  2
BU964562|BU964562.1 sas99a12.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-12311 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56   6e-04  1
BE800797|BE800797.1 sq98c12.y1 Gm-c1049 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1049-1199 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56   6e-04  1
AW397479|AW397479.1 sg79d05.y1 Gm-c1007 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1007-2626 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56   6e-04  1
BG790353|BG790353.1 sae68c10.y1 Gm-c1064 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1064-3212 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56   6e-04  1
BI310160|BI310160.1 EST5311910 GESD Medicago truncatula cDNA clone pGESD7E12 5' end, mRNA sequence.    40   0.002  2
BJ128689|BJ128689.1 Caenorhabditis elegans cDNA clone:yk1021g02 : 3' end, single read.    54   0.002  1
AU216378|AU216378.1 Caenorhabditis elegans cDNA clone:yk837a10 : 3' end, single read.    54   0.002  1
AU212562|AU212562.1 Caenorhabditis elegans cDNA clone:yk786h01 : 3' end, single read.    54   0.002  1
dna update 2004. 3.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF676817_1(EF676817|pid:none) Picea sitchensis clone WS02753_I09...   202   8e-51
EF085049_1(EF085049|pid:none) Picea sitchensis clone WS0297_P09 ...   202   8e-51
BT071197_1(BT071197|pid:none) Picea sitchensis clone WS0279_A10 ...   198   9e-50
AF251042_1(AF251042|pid:none) Mus musculus rab GDP dissociation ...   198   9e-50
AF130987_1(AF130987|pid:none) Rattus norvegicus RAB GDP dissocia...   197   3e-49
(O97555) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   197   3e-49
(P50398) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   197   3e-49
X74402_1(X74402|pid:none) R.norvegicus rab GDI alpha mRNA.            197   3e-49
BC095329_1(BC095329|pid:none) Danio rerio GDP dissociation inhib...   197   3e-49
A54091(A54091;S35964;A37996)rab GDP dissociation inhibitor alpha...   196   3e-49
protein update 2009. 4.21
PSORT 

 psg: 0.91  gvh: 0.50  alm: 0.34  top: 0.30  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH224 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFH224Z
3' end seq. 
>AFH224Z.Seq
----------CCATTATATGGTTTAGGGGANATTACCACAAGCATTTGCCCGTTGATCTG
CTATCTATGGGTGGNACATATATGCTTNATAAACCANATTGAAAATATTGTTTTCGATGA
AAAAAANAAAATTATTAAAGTTACATCTGAAGGTNAAACTGTAACATGTGATAAAATTGT
TTGTGATCCATCATATTTCCCAAATAAAGTTCAAAAGAAAGGTAAAATTATTCGTTCAAT
TTGTATTTTGAATGGTCCAATTCCATCTGCTGATAAGAAATTTGAATCACTCCAAATCAT
TATCCCACAAAAACAAGTTAAACGTCAATCAGATATGTACGTTGGTGTTATGTCATCCGC
TCATTGTGTCTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCACTGTTGAAACCAA
TGAACCAGAGAAAGAATTAGCACCAGCTTACGCTTTATTAGGTCCAATTCAAGAGAAATT
TGTCTATATTTCTGATTTCATTGAACCAATTGCTGATGGTACTGCCGACAATGTTTTCAT
CTCAAAATCATATGATGCTACCTCTCACTTTGAAACCACTTGTGAAGATGTCATGGATAT
GTACAAGAGAATCACNGGTAAAGATT
Length of 3' end seq. 616
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -