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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHP324
Library CH
(Link to library)
Clone ID CHP324
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16255-1|Contig-U16524-1
Original site URL
Representative seq. ID CHP324P
(Link to Original site)
Representative DNA sequence 
>CHP324 (CHP324Q) /CSM/CH/CHP3-A/CHP324Q.Seq.d/
CTGTTGGCCTACTGGNAACATTAAATATTAATAATTAGNATACATTGAAAAAAAAGNAAA
TAATAATAATTAAGAATAAAAAAAAAAAAAAANCTTTANTANCTTTTCANTNGTTTTTAA
AAAAAAAAXXXXXXXXXXCTGGCAATTACACCCAGAACATTGTGGCGGACGTCCAAATAA
GATTAAAGTCATCTGTGTTCCATCACCAAAAGGTACTTGCAAGACTGTTCAATGTCCAAA
AGGTTACAAATGCAAACTCTATGCTGATGGTCCAACATGTGTAAAGATTGAAAAACCAAA
ATGTCTCACTTGTAAAGATATGCATTGTGAGTCAAATGGATTACTCTGTGTCTTGACTCC
ACAAAAGAAGACTGATGAAGAATGTTGTCCAATTATTCCAATCTGTATCAATCCATCAAC
TATTGCCGCATCCACTATTGCCACTACAACTGCATCAACTCGTCACTCAACTGCCTCAAC
AATTGCCTCATTAGTCACTGGTACCACAAGTGGTGGTGGTGGTATGGGTTCATTTGGTGG
TCGATCCGATGAAGAATCAAGTGATCCAAATGCTATACTTGGTCTCTTTGAAGACGATAT
TTTCTGGGGTGATAATGATGAATACTTTAGTGATAACTACCGTTATGTTGATGAAGAAGA
TATTGATGAAGATTTCAATGGTAATGATGAATTTGAAATTGATGTTGAAGGTGATTT
sequence update 2002.10.25
Translated Amino Acid sequence 
vgllxtlninn*XTLKKKXIIIIKNKKKKKXLXXFXXFLKKK---

---WQLHPEHCGGRPNKIKVICVPSPKGTCKTVQCPKGYKCKLYADGPTCVKIEKPKCLT
CKDMHCESNGLLCVLTPQKKTDEECCPIIPICINPSTIAASTIATTTASTRHSTASTIAS
LVTGTTSGGGGMGSFGGRSDEESSDPNAILGLFEDDIFWGDNDEYFSDNYRYVDEEDIDE
DFNGNDEFEIDVEGD
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxh*iliixih*kkxk***lrikkkkxlxxlfxxf*kk---

---laitprtlwrtsk*d*shlcsitkrylqdcsmskrlqmqtlc*wsnmckd*ktkmsh
l*ryal*vkwitlcldstked**rmlsnysnlyqsinycrihychyncinsslnclnncl
ishwyhkwwwwygfiwwsir*rik*skcytwsl*rryflg****il***lplc**rry**
rfqw***i*n*c*r*f

Frame B:
cwptgniky**lxyiekkxnnnn*e*kkkkxfxxfsxvfkkk---

---WQLHPEHCGGRPNKIKVICVPSPKGTCKTVQCPKGYKCKLYADGPTCVKIEKPKCLT
CKDMHCESNGLLCVLTPQKKTDEECCPIIPICINPSTIAASTIATTTASTRHSTASTIAS
LVTGTTSGGGGMGSFGGRSDEESSDPNAILGLFEDDIFWGDNDEYFSDNYRYVDEEDIDE
DFNGNDEFEIDVEGD

Frame C:
vgllxtlninn*XTLKKKXIIIIKNKKKKKXLXXFXXFLKKK---

---gnytqnivadvqirlkssvfhhqkvlarlfnvqkvtnansmlmvqhv*rlknqnvsl
vkicivsqmdysvs*lhkrrlmknvvqlfqsvsihqllphpllplqlhqlvtqlpqqlph
*slvpqvvvvvwvhlvvdpmknqviqmlylvslktifsgvimmntlvittvmlmkkilmk
ismvmmnlklmlkvi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSM572 (SSM572Q) /CSM/SS/SSM5-C/SSM572Q.Seq.d/                       1148   0.0
SSC714 (SSC714Q) /CSM/SS/SSC7-A/SSC714Q.Seq.d/                       1148   0.0
SLA758 (SLA758Q) /CSM/SL/SLA7-C/SLA758Q.Seq.d/                       1148   0.0
SFH118 (SFH118Q) /CSM/SF/SFH1-A/SFH118Q.Seq.d/                       1148   0.0
SFF843 (SFF843Q) /CSM/SF/SFF8-B/SFF843Q.Seq.d/                       1148   0.0
SFF467 (SFF467Q) /CSM/SF/SFF4-C/SFF467Q.Seq.d/                       1148   0.0
SFF412 (SFF412Q) /CSM/SF/SFF4-A/SFF412Q.Seq.d/                       1148   0.0
CHP324 (CHP324Q) /CSM/CH/CHP3-A/CHP324Q.Seq.d/                       1148   0.0
CFH395 (CFH395Q) /CSM/CF/CFH3-D/CFH395Q.Seq.d/                       1148   0.0
CFH359 (CFH359Q) /CSM/CF/CFH3-C/CFH359Q.Seq.d/                       1148   0.0
own update 2004. 5. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26238|M26238.1 D.discoideum spore coat protein SP70 gene, complete cds.  1148   0.0    2
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.  1148   0.0    10
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   9e-08  14
M98546|M98546.1 Dictyostelium discoideum spore coat protein (cotB) gene, 5' end and promoter region.    44   1e-06  2
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   9e-05  15
AC116919|AC116919.2 Dictyostelium discoideum chromosome 2 map complement(6245035-6206343) strain AX4, complete sequence.    32   3e-04  9
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    34   0.001  12
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   0.004  14
AC151520|AC151520.3 Medicago truncatula chromosome 2 BAC clone mte1-34o12, complete sequence.    38   0.004  6
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   0.005  15
dna update 2005. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   289   6e-77
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    60   8e-08
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    60   8e-08
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    50   7e-05
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        49   1e-04
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    49   1e-04
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    44   0.006
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...    41   0.030
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    41   0.030
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    40   0.068
protein update 2009. 4.10
PSORT 

 psg: 0.28  gvh: 0.35  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.08
 nuc: 0.33  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for CHP324 is nuc
5' end seq. ID CHP324F
5' end seq. 
>CHP324F.Seq
CTGTTGGCCTACTGGNAACATTAAATATTAATAATTAGNATACATTGAAAAAAAAGNAAA
TAATAATAATTAAGAATAAAAAAAAAAAAAAANCTTTANTANCTTTTCANTNGTTTTTAA
AAAAAAAANNNNNNNNNN
Length of 5' end seq. 138
3' end seq. ID CHP324Z
3' end seq. 
>CHP324Z.Seq
NNNNNNNNNNCTGGCAATTACACCCAGAACATTGTGGCGGACGTCCAAATAAGATTAAAG
TCATCTGTGTTCCATCACCAAAAGGTACTTGCAAGACTGTTCAATGTCCAAAAGGTTACA
AATGCAAACTCTATGCTGATGGTCCAACATGTGTAAAGATTGAAAAACCAAAATGTCTCA
CTTGTAAAGATATGCATTGTGAGTCAAATGGATTACTCTGTGTCTTGACTCCACAAAAGA
AGACTGATGAAGAATGTTGTCCAATTATTCCAATCTGTATCAATCCATCAACTATTGCCG
CATCCACTATTGCCACTACAACTGCATCAACTCGTCACTCAACTGCCTCAACAATTGCCT
CATTAGTCACTGGTACCACAAGTGGTGGTGGTGGTATGGGTTCATTTGGTGGTCGATCCG
ATGAAGAATCAAGTGATCCAAATGCTATACTTGGTCTCTTTGAAGACGATATTTTCTGGG
GTGATAATGATGAATACTTTAGTGATAACTACCGTTATGTTGATGAAGAAGATATTGATG
AAGATTTCAATGGTAATGATGAATTTGAAATTGATGTTGAAGGTGATTT
Length of 3' end seq. 589
Connected seq. ID CHP324P
Connected seq. 
>CHP324P.Seq
CTGTTGGCCTACTGGNAACATTAAATATTAATAATTAGNATACATTGAAAAAAAAGNAAA
TAATAATAATTAAGAATAAAAAAAAAAAAAAANCTTTANTANCTTTTCANTNGTTTTTAA
AAAAAAAA----------CTGGCAATTACACCCAGAACATTGTGGCGGACGTCCAAATAA
GATTAAAGTCATCTGTGTTCCATCACCAAAAGGTACTTGCAAGACTGTTCAATGTCCAAA
AGGTTACAAATGCAAACTCTATGCTGATGGTCCAACATGTGTAAAGATTGAAAAACCAAA
ATGTCTCACTTGTAAAGATATGCATTGTGAGTCAAATGGATTACTCTGTGTCTTGACTCC
ACAAAAGAAGACTGATGAAGAATGTTGTCCAATTATTCCAATCTGTATCAATCCATCAAC
TATTGCCGCATCCACTATTGCCACTACAACTGCATCAACTCGTCACTCAACTGCCTCAAC
AATTGCCTCATTAGTCACTGGTACCACAAGTGGTGGTGGTGGTATGGGTTCATTTGGTGG
TCGATCCGATGAAGAATCAAGTGATCCAAATGCTATACTTGGTCTCTTTGAAGACGATAT
TTTCTGGGGTGATAATGATGAATACTTTAGTGATAACTACCGTTATGTTGATGAAGAAGA
TATTGATGAAGATTTCAATGGTAATGATGAATTTGAAATTGATGTTGAAGGTGATTT
Length of connected seq. 707
Full length Seq ID -
Full length Seq. -
Length of full length seq. -