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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHP321
Library CH
(Link to library)
Clone ID CHP321
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15525-1
Original site URL
Representative seq. ID CHP321P
(Link to Original site)
Representative DNA sequence 
>CHP321 (CHP321Q) /CSM/CH/CHP3-A/CHP321Q.Seq.d/
CTGTTGGCCTACTGGNATTTTTGAATTTGTTGTTCAAAATAAATCCTTCTATACATATAC
ACTAAAAGNATGTCTCAAATGATTAGACCACCAATTGTACTTTTAAAAGAAGGTACAGAT
ACATCACAAGGTTTACCACAATTAATTAGTAATATAAATGCATGTTGTGCAATCGTAGAT
ACAGTACGTACAACATTAGGACCACGTGGTATGGATAAATTAATCTATCAAAGCGAAAGA
CAGGTTACCATATCAAACGATGGTGCCACCGTTATGAAATTATTAGATATTGTACATCCA
GCAGCACGTACATTGGTAGATATAGCAAAGAGTCAAGATTCAGAAGTTGGTGATGGTACA
ACCAGTGTAGTGATTTTAGCAGGTGAATTCCTTAAAGCAGCCAAACCATTCCTCGAGGAG
GGTATTCATCCACAAATTATAATCCGTGCCTTTAGAAGTGCATGTGAATTGGCTAAACAA
AAGATTCAAGAGTTGAGCGTAGATATCAAACCAGAAAACATGAGAGAATTCCTCGAAAAG
TGTGCTTCAACCTCGATGAACTCTAAATTAATCGCCTCTCACAAACAATTCTTTAGTAAA
ATGGTGGTAGACGCCGTACAATTGTTGGACGACAATATCGGTCTCGATATGATTGGCATC
AAGAAGGXXXXXXXXXXTGATGATGACATTCGTCGTGTTTGTCGTGCCACCGGTGCTGCC
ATCCAAAACACTACATCCAATATCATCCCAGACGTAATTGGTACCTGCGACCTCTTTGAA
GAGGTTCAAGTAGGTGGTCAACGTTACAACTTGTTCACTGGCTGCACAATGACCCAAACC
GCCACTATCATCCTTCGTGGTGGTGGTGAACAATTCATCGATGAGGCCGAACGTTCCCTT
CACGATTCTATCATGATCGTTAGACGTGCCCGTAAACATAGATCAGTGGTTGCCGGTGGT
GGTGCTATTGAAATGGAAGTCTCTAAATATCTTCGTGACTATTCCCTCTCCATTGAAGGT
AAGAAGCAACTCCTCATCAATGCTTTCGCTAAAGCACTCGAAGTCATCCCAAGACAAATC
GCCGATAATGCTGGTTTCGACTCTACCGATATCCTCAATCAATTAAGACAAAAACATGCT
CAAGGTGAAAAATGGTTTGGTGTTGACATTGTAAACGAAGGTATTTGTGATACCTATGAA
TCTGCAATTTGGGAACCATCTCTCGTTAAACTCAATTCAATCGTTGCCGCAACTGAAGCA
ACTTGTTTAATCCTNCTNCTGTTGATGAAACCGTTCAAAATAATCAAGCTGAACAAGNTN
CAAGCTGGTNCCACAAATTAATAACCAAACACGA
sequence update 2002.10.25
Translated Amino Acid sequence 
llaywxf*iccsk*ILLYIYTKXMSQMIRPPIVLLKEGTDTSQGLPQLISNINACCAIVD
TVRTTLGPRGMDKLIYQSERQVTISNDGATVMKLLDIVHPAARTLVDIAKSQDSEVGDGT
TSVVILAGEFLKAAKPFLEEGIHPQIIIRAFRSACELAKQKIQELSVDIKPENMREFLEK
CASTSMNSKLIASHKQFFSKMVVDAVQLLDDNIGLDMIGIKK---

---DDDIRRVCRATGAAIQNTTSNIIPDVIGTCDLFEEVQVGGQRYNLFTGCTMTQTATI
ILRGGGEQFIDEAERSLHDSIMIVRRARKHRSVVAGGGAIEMEVSKYLRDYSLSIEGKKQ
LLINAFAKALEVIPRQIADNAGFDSTDILNQLRQKHAQGEKWFGVDIVNEGICDTYESAI
WEPSLVKLNSIVAATEATCLILLLLMKPFKIIKLNKXQAGXTN**pnt
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxf*iccsk*ILLYIYTKXMSQMIRPPIVLLKEGTDTSQGLPQLISNINACCAIVD
TVRTTLGPRGMDKLIYQSERQVTISNDGATVMKLLDIVHPAARTLVDIAKSQDSEVGDGT
TSVVILAGEFLKAAKPFLEEGIHPQIIIRAFRSACELAKQKIQELSVDIKPENMREFLEK
CASTSMNSKLIASHKQFFSKMVVDAVQLLDDNIGLDMIGIKK---

---***hssclschrcchpkhyiqyhprrnwylrpl*rgssrwstlqlvhwlhndpnrhy
hpswww*tihr*grtfpsrfyhdr*tcp*t*isgcrwwcy*ngsl*iss*lfplh*r*ea
tphqcfr*strshpktnrr*cwfrlyrypqsiktktcsr*kmvwc*hckrryl*yl*icn
lgtisr*tqfnrcrn*snlfnpxxvdetvqnnqaeqxxswxhklitkh

Frame B:
cwptgifefvvqnksfytytlkxclk*ldhqlyf*kkvqihhkvyhn*lvi*mhvvqs*i
qyvqh*dhvvwin*sikakdrlpyqtmvppl*ny*ilyiqqhvhw*i*qrvkiqklvmvq
pv**f*qvnslkqpnhssrrvfihkl*svplevhvnwlnkrfks*a*isnqkt*enssks
vlqpr*tln*spltnnslvkww*tpyncwttisvsi*lasrr---

---DDDIRRVCRATGAAIQNTTSNIIPDVIGTCDLFEEVQVGGQRYNLFTGCTMTQTATI
ILRGGGEQFIDEAERSLHDSIMIVRRARKHRSVVAGGGAIEMEVSKYLRDYSLSIEGKKQ
LLINAFAKALEVIPRQIADNAGFDSTDILNQLRQKHAQGEKWFGVDIVNEGICDTYESAI
WEPSLVKLNSIVAATEATCLILLLLMKPFKIIKLNKXQAGXTN**pnt

Frame C:
vgllxflnllfkinpsihih*kxvsnd*ttnctfkrryryitrfttin**ykcmlcnrry
stynirttwyg*inlskrktgyhikrwchryeiiryctssstyigryskesrfrsw*wyn
qcsdfsr*ip*ssqtiprggysstnynpcl*kcm*ig*tkdsrverryqtrkheriprkv
cfnldel*inrlsqtil**nggrrrtivgrqyrsrydwhqe---

---mmtfvvfvvppvlpsktlhpissqt*lvpatslkrfk*vvnvttcslaaq*pkppls
sfvvvvnnssmrpnvpftils*sldvpvnidqwlpvvvllkwkslnifvtipsplkvrsn
sssmlslkhskssqdkspimlvstlpissin*dknmlkvknglvltl*tkvfvipmnlqf
gnhlslnsiqslpqlkqlv*sxxc**nrsk*ss*txxklvpqinnqtr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHP321 (CHP321Q) /CSM/CH/CHP3-A/CHP321Q.Seq.d/                       2583   0.0
SFB523 (SFB523Q) /CSM/SF/SFB5-A/SFB523Q.Seq.d/                       1271   0.0
CFI715 (CFI715Q) /CSM/CF/CFI7-A/CFI715Q.Seq.d/                       1271   0.0
SFK263 (SFK263Q) /CSM/SF/SFK2-C/SFK263Q.Seq.d/                       1267   0.0
AFN374 (AFN374Q) /CSM/AF/AFN3-D/AFN374Q.Seq.d/                       1265   0.0
CFI614 (CFI614Q) /CSM/CF/CFI6-A/CFI614Q.Seq.d/                       1263   0.0
AFC815 (AFC815Q) /CSM/AF/AFC8-A/AFC815Q.Seq.d/                       1263   0.0
AFA623 (AFA623Q) /CSM/AF/AFA6-A/AFA623Q.Seq.d/                       1263   0.0
CFJ411 (CFJ411Q) /CSM/CF/CFJ4-A/CFJ411Q.Seq.d/                       1261   0.0
SFK806 (SFK806Q) /CSM/SF/SFK8-A/SFK806Q.Seq.d/                       1257   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U43896|U43896.1 Dictyostelium discoideum molecular chaperone Dd-TCP1 mRNA, complete cds.  1144   0.0    3
G65009|G65009.1 DH0182 Dictyostelium discoideum ( P.Dear ) Dictyostelium discoideum STS genomic 5', sequence tagged site.   270   7e-83  2
U46030|U46030.1 Tetrahymena pyriformis CCTeta chaperonin subunit (TpCCTeta) gene, complete cds.    46   2e-14  4
AZ931214|AZ931214.1 474.dhz63d04.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz63d04.s1, DNA sequence.    56   1e-13  3
AX489069|AX489069.1 Sequence 6369 from Patent WO02053728.          46   3e-11  4
AR550554|AR550554.1 Sequence 5685 from patent US 6747137.          46   2e-10  4
DT740965|DT740965.1 ---.    64   6e-08  2
AF427597|AF427597.1 Artemia franciscana chaperonin subunit 1 (CHP) mRNA, complete cds.    40   7e-08  4
BU719561|BU719561.1 SJM2AAE07 SJM Schistosoma japonicum cDNA, mRNA sequence.    44   3e-07  4
DN636147| chaperonin contain, mRNA sequence.    36   2e-06  4
dna update 2005. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U43896_1(U43896|pid:none) Dictyostelium discoideum molecular cha...   389   e-107
AF494046_1(AF494046|pid:none) Physarum polycephalum chaperonin c...   301   3e-80
BC042312_1(BC042312|pid:none) Xenopus laevis hypothetical LOC495...   273   1e-71
AK077781_1(AK077781|pid:none) Mus musculus adult male thymus cDN...   271   4e-71
AK168272_1(AK168272|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...   271   4e-71
(P80313) RecName: Full=T-complex protein 1 subunit eta;         ...   271   4e-71
A45921_1(A45921|pid:none) Sequence 60 from Patent WO9520654.          271   4e-71
AF506229_1(AF506229|pid:none) Danio rerio chaperonin-containing ...   269   1e-70
BC077927_1(BC077927|pid:none) Xenopus laevis chaperonin containi...   269   1e-70
BC045074_1(BC045074|pid:none) Xenopus laevis chaperonin containi...   269   1e-70
protein update 2009. 4.10
PSORT 

 psg: 0.69  gvh: 0.49  alm: 0.27  top: 0.87  tms: 0.07  mit: 0.34  mip: 0.04
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: Golgi
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CHP321 is cyt
5' end seq. ID CHP321F
5' end seq. 
>CHP321F.Seq
CTGTTGGCCTACTGGNATTTTTGAATTTGTTGTTCAAAATAAATCCTTCTATACATATAC
ACTAAAAGNATGTCTCAAATGATTAGACCACCAATTGTACTTTTAAAAGAAGGTACAGAT
ACATCACAAGGTTTACCACAATTAATTAGTAATATAAATGCATGTTGTGCAATCGTAGAT
ACAGTACGTACAACATTAGGACCACGTGGTATGGATAAATTAATCTATCAAAGCGAAAGA
CAGGTTACCATATCAAACGATGGTGCCACCGTTATGAAATTATTAGATATTGTACATCCA
GCAGCACGTACATTGGTAGATATAGCAAAGAGTCAAGATTCAGAAGTTGGTGATGGTACA
ACCAGTGTAGTGATTTTAGCAGGTGAATTCCTTAAAGCAGCCAAACCATTCCTCGAGGAG
GGTATTCATCCACAAATTATAATCCGTGCCTTTAGAAGTGCATGTGAATTGGCTAAACAA
AAGATTCAAGAGTTGAGCGTAGATATCAAACCAGAAAACATGAGAGAATTCCTCGAAAAG
TGTGCTTCAACCTCGATGAACTCTAAATTAATCGCCTCTCACAAACAATTCTTTAGTAAA
ATGGTGGTAGACGCCGTACAATTGTTGGACGACAATATCGGTCTCGATATGATTGGCATC
AAGAAGGNNNNNNNNNN
Length of 5' end seq. 677
3' end seq. ID CHP321Z
3' end seq. 
>CHP321Z.Seq
NNNNNNNNNNTGATGATGACATTCGTCGTGTTTGTCGTGCCACCGGTGCTGCCATCCAAA
ACACTACATCCAATATCATCCCAGACGTAATTGGTACCTGCGACCTCTTTGAAGAGGTTC
AAGTAGGTGGTCAACGTTACAACTTGTTCACTGGCTGCACAATGACCCAAACCGCCACTA
TCATCCTTCGTGGTGGTGGTGAACAATTCATCGATGAGGCCGAACGTTCCCTTCACGATT
CTATCATGATCGTTAGACGTGCCCGTAAACATAGATCAGTGGTTGCCGGTGGTGGTGCTA
TTGAAATGGAAGTCTCTAAATATCTTCGTGACTATTCCCTCTCCATTGAAGGTAAGAAGC
AACTCCTCATCAATGCTTTCGCTAAAGCACTCGAAGTCATCCCAAGACAAATCGCCGATA
ATGCTGGTTTCGACTCTACCGATATCCTCAATCAATTAAGACAAAAACATGCTCAAGGTG
AAAAATGGTTTGGTGTTGACATTGTAAACGAAGGTATTTGTGATACCTATGAATCTGCAA
TTTGGGAACCATCTCTCGTTAAACTCAATTCAATCGTTGCCGCAACTGAAGCAACTTGTT
TAATCCTNCTNCTGTTGATGAAACCGTTCAAAATAATCAAGCTGAACAAGNTNCAAGCTG
GTNCCACAAATTAATAACCAAACACGA
Length of 3' end seq. 687
Connected seq. ID CHP321P
Connected seq. 
>CHP321P.Seq
CTGTTGGCCTACTGGNATTTTTGAATTTGTTGTTCAAAATAAATCCTTCTATACATATAC
ACTAAAAGNATGTCTCAAATGATTAGACCACCAATTGTACTTTTAAAAGAAGGTACAGAT
ACATCACAAGGTTTACCACAATTAATTAGTAATATAAATGCATGTTGTGCAATCGTAGAT
ACAGTACGTACAACATTAGGACCACGTGGTATGGATAAATTAATCTATCAAAGCGAAAGA
CAGGTTACCATATCAAACGATGGTGCCACCGTTATGAAATTATTAGATATTGTACATCCA
GCAGCACGTACATTGGTAGATATAGCAAAGAGTCAAGATTCAGAAGTTGGTGATGGTACA
ACCAGTGTAGTGATTTTAGCAGGTGAATTCCTTAAAGCAGCCAAACCATTCCTCGAGGAG
GGTATTCATCCACAAATTATAATCCGTGCCTTTAGAAGTGCATGTGAATTGGCTAAACAA
AAGATTCAAGAGTTGAGCGTAGATATCAAACCAGAAAACATGAGAGAATTCCTCGAAAAG
TGTGCTTCAACCTCGATGAACTCTAAATTAATCGCCTCTCACAAACAATTCTTTAGTAAA
ATGGTGGTAGACGCCGTACAATTGTTGGACGACAATATCGGTCTCGATATGATTGGCATC
AAGAAGG----------TGATGATGACATTCGTCGTGTTTGTCGTGCCACCGGTGCTGCC
ATCCAAAACACTACATCCAATATCATCCCAGACGTAATTGGTACCTGCGACCTCTTTGAA
GAGGTTCAAGTAGGTGGTCAACGTTACAACTTGTTCACTGGCTGCACAATGACCCAAACC
GCCACTATCATCCTTCGTGGTGGTGGTGAACAATTCATCGATGAGGCCGAACGTTCCCTT
CACGATTCTATCATGATCGTTAGACGTGCCCGTAAACATAGATCAGTGGTTGCCGGTGGT
GGTGCTATTGAAATGGAAGTCTCTAAATATCTTCGTGACTATTCCCTCTCCATTGAAGGT
AAGAAGCAACTCCTCATCAATGCTTTCGCTAAAGCACTCGAAGTCATCCCAAGACAAATC
GCCGATAATGCTGGTTTCGACTCTACCGATATCCTCAATCAATTAAGACAAAAACATGCT
CAAGGTGAAAAATGGTTTGGTGTTGACATTGTAAACGAAGGTATTTGTGATACCTATGAA
TCTGCAATTTGGGAACCATCTCTCGTTAAACTCAATTCAATCGTTGCCGCAACTGAAGCA
ACTTGTTTAATCCTNCTNCTGTTGATGAAACCGTTCAAAATAATCAAGCTGAACAAGNTN
CAAGCTGGTNCCACAAATTAATAACCAAACACGA
Length of connected seq. 1344
Full length Seq ID -
Full length Seq. -
Length of full length seq. -