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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHP252
Library CH
(Link to library)
Clone ID CHP252
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID CHP252P
(Link to Original site)
Representative DNA sequence 
>CHP252 (CHP252Q) /CSM/CH/CHP2-C/CHP252Q.Seq.d/
CTGTTGGCCTACTGGNAATTACTGCCCCAGNAAAACGCTATTGGTGGTATTTACTCTGTA
CTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTC
TCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA
CTTTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAATAATAAA
AAAAAAAXXXXXXXXXXAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTA
CTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTC
TCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATTCTTGACAAATTNCCACGAAAAACTCTAAACAATTTAAGTCG
CACTTGAGTTTTCAAATATAGTAANCAANTTAAAAAAANTTTAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptgnycpxktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvp
tlpvklshnvssiiglqlvlstkirkplksl*pperekv*PQKSQILTNSTKNSKQFKSH
FEFSNIVIKSKKNFKKKIIKKK---

---ITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTA
GQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDS*qixtknskqfkshls
fqi**xx*kxfkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxllpqxnaiggiysvlnrrrgivigeerrigsplfsvkahlpvlesfgftadlrs
htagqafpqcvfdhwasigvvnkdkkatevalatrkrkglapeipdldkfhekl*ti*va
l*vfkysnqi*kkf*kknnkkk---

---nycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphc
rssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprfltnxhekl*ti*vale
fsnivxxlkkx*kk

Frame B:
cwptgnycpxktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvp
tlpvklshnvssiiglqlvlstkirkplksl*pperekv*PQKSQILTNSTKNSKQFKSH
FEFSNIVIKSKKNFKKKIIKKK---

---ITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTA
GQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDS*qixtknskqfkshls
fqi**xx*kxfkkk

Frame C:
vgllxitapxkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfp
hcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrt
lsfqi**snlkkilkkk**kk---

---llpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlp
vklshnvssiiglqlvlstkirkplksl*pperekv*pqksqildkxprktlnnlsrt*v
fkysxqxkkxlkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHP252 (CHP252Q) /CSM/CH/CHP2-C/CHP252Q.Seq.d/                        763   0.0
VSB147 (VSB147Q) /CSM/VS/VSB1-B/VSB147Q.Seq.d/                        728   0.0
VHG546 (VHG546Q) /CSM/VH/VHG5-B/VHG546Q.Seq.d/                        728   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                        722   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                        722   0.0
VHO504 (VHO504Q) /CSM/VH/VHO5-A/VHO504Q.Seq.d/                        722   0.0
VHO104 (VHO104Q) /CSM/VH/VHO1-A/VHO104Q.Seq.d/                        722   0.0
VHI806 (VHI806Q) /CSM/VH/VHI8-A/VHI806Q.Seq.d/                        722   0.0
VHD305 (VHD305Q) /CSM/VH/VHD3-A/VHD305Q.Seq.d/                        722   0.0
VHD106 (VHD106Q) /CSM/VH/VHD1-A/VHD106Q.Seq.d/                        722   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   561   0.0    3
CK811111|CK811111.1 Rasgsc6510 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   2e-12  2
CK807987|CK807987.1 Rasgsc0236 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   2e-12  2
CK808524|CK808524.1 Rasgsc1215 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   2e-12  2
CK808261|CK808261.1 Rasgsc0738 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   2e-12  2
CK808525|CK808525.1 Rasgsc1216 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   2e-12  2
BX569353|BX569353.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse99a12_p1c.    84   5e-12  1
BX557472|BX557472.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse32f02_p1c.    84   5e-12  1
BX566526|BX566526.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse82g03_p1c.    84   5e-12  1
BX565591|BX565591.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse78d05_p1c.    84   5e-12  1
dna update 2005. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   113   6e-24
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   106   8e-22
AY831447_1(AY831447|pid:none) Monocercomonoides sp. PA203 transl...   105   1e-21
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   105   1e-21
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   105   2e-21
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   105   2e-21
(A5DI11) RecName: Full=Elongation factor 2;          Short=EF-2;      105   2e-21
(Q875S0) RecName: Full=Elongation factor 2;          Short=EF-2;...   104   3e-21
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   104   4e-21
AF544973_1(AF544973|pid:none) Ctenopharyngodon idella elongation...   104   4e-21
protein update 2009. 4.10
PSORT 

 psg: 0.76  gvh: 0.29  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.11
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CHP252 is nuc
5' end seq. ID CHP252F
5' end seq. 
>CHP252F.Seq
CTGTTGGCCTACTGGNAATTACTGCCCCAGNAAAACGCTATTGGTGGTATTTACTCTGTA
CTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTC
TCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA
CTTTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAATAATAAA
AAAAAAANNNNNNNNNN
Length of 5' end seq. 437
3' end seq. ID CHP252Z
3' end seq. 
>CHP252Z.Seq
NNNNNNNNNNAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATC
GTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCA
AAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTG
CCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACA
AAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAG
AAATCCCAGATTCTTGACAAATTNCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGA
GTTTTCAAATATAGTAANCAANTTAAAAAAANTTTAAAAAAAAA
Length of 3' end seq. 404
Connected seq. ID CHP252P
Connected seq. 
>CHP252P.Seq
CTGTTGGCCTACTGGNAATTACTGCCCCAGNAAAACGCTATTGGTGGTATTTACTCTGTA
CTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTC
TCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA
CTTTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAATAATAAA
AAAAAAA----------AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTA
CTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTC
TCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATTCTTGACAAATTNCCACGAAAAACTCTAAACAATTTAAGTCG
CACTTGAGTTTTCAAATATAGTAANCAANTTAAAAAAANTTTAAAAAAAAA
Length of connected seq. 821
Full length Seq ID -
Full length Seq. -
Length of full length seq. -