>AFH142 (AFH142Q) /CSM/AF/AFH1-B/AFH142Q.Seq.d/
TATCATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAG
TTATATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATA
GATTTCTTTTTTTTTTTTTTTTTCCCCXXXXXXXXXXTTCTCCTTTTGCCATCTCTAATG
CTGTAATACCCATACTCCAAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTT
CAGGTGCCATCCAAAATGGGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTA
ATTGACCACTAACACCAAAATCAGCTAATTTAACATCACCGCTTGCTGAAAGTAAAACAT
TTGCAGCTTTAATATCTCTGTGAATTTTACCTTCTGAATGTAAATATTCCAAACCTTTTA
ATAACTCTCTTAAAATAATTGCAATATATCCTTCGTCGAATGGACCTGGTTTCATCAAAT
CTAAAACTGAACCACCTGCAAGATATTCCATAATAATCCATAATTTTGAACCTTTTAAAA
ATGAACCAAAATATTTTGTTACAAATGGACTTTCACATTGAGAGAGTACATTGNTTTCTT
GTTGAATATCTTCAATTTCATCCTCTGCATCTTCAAGATCAANTGTTTTTATTGCTATTG
TTTCATTTGTTTTTTTATTGATACCTTTGAATACTTCACCAAATGAACCTNNACCAATCT
TCTCGTGTCAGCACATAGAGNTCTTCTGGANCACCTTTGTGTTGCNTGCCATGNTTAGTT
CTNACATTTNACAAATTNGNCATTNATATTCGT

yhkrtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFFFFFFFP---

---SPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFLFVI*svn*pltpksan
ltsplaesktfaalisl*ilpseckyskpfnnslkiiaiypssngpgfiksktepparys
iiihnfepfknepkyfvtnglsh*estlxsc*ississsassrsxvfiaivsfvfllipl
ntspnepxpifscqhiexfwxtfvlxamxssxixqixhxys

Frame A:
yhkrtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFFFFFFFP---

---fsfchl*ccnthtpnisf*iisslfnnfrchpkwgtnkcislchlis*lttntkis*
fnitac*k*nicsfnisvnftf*m*ifqtf**ls*nncnisfvewtwfhqi*n*ttckif
hnnp*f*tf*k*tkifcykwtftlreyixfllnifnfilcifkixcfycycficffidtf
eyftk*txtnllvsahrxllxhlcvachx*fxhxtnxxxif

Frame B:
iinvql*yknnhtfcnssnsyi*iyr*knsnikekkni**isfffffs---

---SPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFLFVI*svn*pltpksan
ltsplaesktfaalisl*ilpseckyskpfnnslkiiaiypssngpgfiksktepparys
iiihnfepfknepkyfvtnglsh*estlxsc*ississsassrsxvfiaivsfvfllipl
ntspnepxpifscqhiexfwxtfvlxamxssxixqixhxys

Frame C:
s*tynyniktithfvivvtvifkyigkktvt*rkrktynrflfffffp---

---lllpslml*ypyskyqllnhiqfv**lqvpskmgyqqmyfslsfdqlidh*hqnqli
*hhrllkvkhlql*ylcefyllnvnipnllitllk*lqyilrrmdlvssnlklnhlqdip
**siilnllkmnqnillqmdfhiervhxflveylqfhplhlqdqxfllllfhlffy*yl*
ilhqmnlxqssrvst*xssgxplccxpxlvltfxkxxixir

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH142 (AFH142Q) /CSM/AF/AFH1-B/AFH142Q.Seq.d/                       1181   0.0
VFH696 (VFH696Q) /CSM/VF/VFH6-D/VFH696Q.Seq.d/                       1082   0.0
SFJ867 (SFJ867Q) /CSM/SF/SFJ8-C/SFJ867Q.Seq.d/                       1055   0.0
SFL213 (SFL213Q) /CSM/SF/SFL2-A/SFL213Q.Seq.d/                       1023   0.0
SFD665 (SFD665Q) /CSM/SF/SFD6-C/SFD665Q.Seq.d/                       1007   0.0
SFH469 (SFH469Q) /CSM/SF/SFH4-C/SFH469Q.Seq.d/                        892   0.0
AFN655 (AFN655Q) /CSM/AF/AFN6-C/AFN655Q.Seq.d/                        860   0.0
SFG377 (SFG377Q) /CSM/SF/SFG3-D/SFG377Q.Seq.d/                        841   0.0
SLG682 (SLG682Q) /CSM/SL/SLG6-D/SLG682Q.Seq.d/                        785   0.0
AFB293 (AFB293Q) /CSM/AF/AFB2-D/AFB293Q.Seq.d/                        676   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF059534|AF059534.1 Dictyostelium discoideum severin kinase mRNA, complete cds.  1003   0.0    2
AF061281|AF061281.1 Dictyostelium discoideum kinase responsive to stress 1-like kinase mRNA, complete cds.    66   7e-25  4
AL547396|AL547396.3 human full-length cDNA 5-PRIME end of clone CS0DI007YP04 of PLACENTA COT 25-NORMALIZED of Homo sapiens (human).    62   2e-23  6
CK578018|CK578018.1 IST_WI5_18384 AD-wrmcDNA library Caenorhabditis elegans cDNA 5' similar to T19A5.2, mRNA sequence.    64   4e-22  3
BJ095939|BJ095939.1 Xenopus laevis cDNA clone:XL152b02, 5' end, single read.    48   4e-19  4
BD196965|BD196965.1 Human signal transduction serine/threonine kinase.    58   3e-18  4
A94741|A94741.1 Sequence 2 from Patent WO9932637.                  58   3e-18  4
AR369644|AR369644.1 Sequence 2 from patent US 6300098.             58   3e-18  4
AR077733|AR077733.1 Sequence 2 from patent US 5962265.             58   3e-18  4
BD196968|BD196968.1 Human signal transduction serine/threonine kinase.    58   3e-18  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O61122) RecName: Full=Serine/threonine-protein kinase svkA;    ...   337   3e-91
BC045867_1(BC045867|pid:none) Danio rerio serine/threonine kinas...   280   5e-74
BT045853_1(BT045853|pid:none) Salmo salar clone ssal-rgf-534-302...   280   5e-74
(Q3SWY6) RecName: Full=Serine/threonine-protein kinase 25;      ...   272   8e-72
BC133508_1(BC133508|pid:none) Bos taurus serine/threonine kinase...   271   1e-71
BC167296_1(BC167296|pid:none) Xenopus tropicalis hypothetical pr...   271   1e-71
BC015793_1(BC015793|pid:none) Homo sapiens serine/threonine kina...   271   2e-71
BT001630_1(BT001630|pid:none) Drosophila melanogaster RE38276 fu...   271   2e-71
AK148041_1(AK148041|pid:none) Mus musculus B16 F10Y cells cDNA, ...   271   2e-71
AK152917_1(AK152917|pid:none) Mus musculus bone marrow macrophag...   271   2e-71

 psg: 1.02  gvh: 0.52  alm: 0.33  top: 0.23  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH142 is cyt

>AFH142F.Seq
TATCATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAG
TTATATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATA
GATTTCTTTTTTTTTTTTTTTTTCCCC----------

>AFH142Z.Seq
----------TTCTCCTTTTGCCATCTCTAATGCTGTAATACCCATACTCCAAATATCAG
CTTTTGAATCATATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAAATGGGGTACCAA
CAAATGTATTTCTCTTTGTCATTTGATCAGTTAATTGACCACTAACACCAAAATCAGCTA
ATTTAACATCACCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATATCTCTGTGAATTT
TACCTTCTGAATGTAAATATTCCAAACCTTTTAATAACTCTCTTAAAATAATTGCAATAT
ATCCTTCGTCGAATGGACCTGGTTTCATCAAATCTAAAACTGAACCACCTGCAAGATATT
CCATAATAATCCATAATTTTGAACCTTTTAAAAATGAACCAAAATATTTTGTTACAAATG
GACTTTCACATTGAGAGAGTACATTGNTTTCTTGTTGAATATCTTCAATTTCATCCTCTG
CATCTTCAAGATCAANTGTTTTTATTGCTATTGTTTCATTTGTTTTTTTATTGATACCTT
TGAATACTTCACCAAATGAACCTNNACCAATCTTCTCGTGTCAGCACATAGAGNTCTTCT
GGANCACCTTTGTGTTGCNTGCCATGNTTAGTTCTNACATTTNACAAATTNGNCATTNAT
ATTCGT

>AFH142P.Seq
TATCATAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAG
TTATATTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATA
GATTTCTTTTTTTTTTTTTTTTTCCCC----------TTCTCCTTTTGCCATCTCTAATG
CTGTAATACCCATACTCCAAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTT
CAGGTGCCATCCAAAATGGGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTA
ATTGACCACTAACACCAAAATCAGCTAATTTAACATCACCGCTTGCTGAAAGTAAAACAT
TTGCAGCTTTAATATCTCTGTGAATTTTACCTTCTGAATGTAAATATTCCAAACCTTTTA
ATAACTCTCTTAAAATAATTGCAATATATCCTTCGTCGAATGGACCTGGTTTCATCAAAT
CTAAAACTGAACCACCTGCAAGATATTCCATAATAATCCATAATTTTGAACCTTTTAAAA
ATGAACCAAAATATTTTGTTACAAATGGACTTTCACATTGAGAGAGTACATTGNTTTCTT
GTTGAATATCTTCAATTTCATCCTCTGCATCTTCAAGATCAANTGTTTTTATTGCTATTG
TTTCATTTGTTTTTTTATTGATACCTTTGAATACTTCACCAAATGAACCTNNACCAATCT
TCTCGTGTCAGCACATAGAGNTCTTCTGGANCACCTTTGTGTTGCNTGCCATGNTTAGTT
CTNACATTTNACAAATTNGNCATTNATATTCGT