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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH123
Library AF
(Link to library)
Clone ID AFH123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFH123P
(Link to Original site)
Representative DNA sequence 
>AFH123 (AFH123Q) /CSM/AF/AFH1-A/AFH123Q.Seq.d/
TGCGGNAATTCCTACGTGNCACTGTTGGCCTACTGGACTTTATTTTTTTTTATCTCTATC
TATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCAGXXXXXXXXXXAATTCAAAGTTGAAAAATGGTTACGATCTTNACTTCAAAACTAAA
AACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTC
ATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCA
TACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACC
AACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAGGCATGTAATGCTCTTTTATTAAAA
GTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCA
TCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGAT
TTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGA
TTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
AXIPTXHCWPTGLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVV
SSFRAAVPSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAI
DKLMIDLDGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFN
VINGGSHAGNKLAMQEFMILP---

---NSKLKNGYDLXFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYT
KLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASW
GVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKT
Translated Amino Acid sequence (All Frames) 
Frame A:
cgnsyvxllaywtlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwcc
fiiqscssircinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscy
r*indrsrwypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiq
cyqwwftcr**isyariydft---

---NSKLKNGYDLXFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYT
KLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASW
GVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKT

Frame B:
AXIPTXHCWPTGLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVV
SSFRAAVPSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAI
DKLMIDLDGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFN
VINGGSHAGNKLAMQEFMILP---

---iqs*kmvtixtsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtl
n*qhqlifkllvmiyllptqkelrlvlkrrhvmlfy*kltklvqslnqfvplltqrmhhg
vswlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vkp

Frame C:
rxflrxtvglldfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlf
hhselqfhqvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpiklls
in**sismvpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsm
lsmvvhmqvin*lcknl*fyq---

---fkvekwlrsxlqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*
insis*ysncw**ftcyqprkn*dwy*kegm*csfiks*pnwfsh*inscrs*lkecimg
chg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH123 (AFH123Q) /CSM/AF/AFH1-A/AFH123Q.Seq.d/                       2113   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1098   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1098   0.0
SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                       1098   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1098   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1098   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1098   0.0
SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/                       1098   0.0
CFD322 (CFD322Q) /CSM/CF/CFD3-A/CFD322Q.Seq.d/                       1098   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1098   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressinggrowth of plasmodium.    76   8e-56  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   8e-56  9
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   8e-56  9
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   7e-54  8
X80474|X80474.1 N.frontalis enolase gene.                          64   2e-53  8
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   5e-48  8
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   8e-47  6
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   3e-43  4
AE014831|AE014831.1 Plasmodium falciparum 3D7 chromosome 10 section 3 of 7 of the complete sequence.    76   9e-42  14
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   2e-38  9
dna update 2004. 3.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   334   4e-90
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   263   6e-69
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   258   3e-67
AF100985_1(AF100985|pid:none) Penaeus monodon phosphopyruvate hy...   256   1e-66
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   254   3e-66
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   254   5e-66
(P42896) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   253   6e-66
FJ664264_1(FJ664264|pid:none) Annona cherimola enolase mRNA, com...   253   8e-66
EF148215_1(EF148215|pid:none) Populus trichocarpa clone WS0128_M...   252   1e-65
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   252   2e-65
protein update 2009. 4.21
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.33  top: 0.47  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: cytoskeletal

>> prediction for AFH123 is cyt
5' end seq. ID AFH123F
5' end seq. 
>AFH123F.Seq
TGCGGNAATTCCTACGTGNCACTGTTGGCCTACTGGACTTTATTTTTTTTTATCTCTATC
TATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCAG----------
Length of 5' end seq. 605
3' end seq. ID AFH123Z
3' end seq. 
>AFH123Z.Seq
----------AATTCAAAGTTGAAAAATGGTTACGATCTTNACTTCAAAACTAAAAACAA
TGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAA
AGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACAC
TAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCC
AGAAAGAATTAAGACTGGTATTGAAAAGAAGGCATGTAATGCTCTTTTATTAAAAGTTAA
CCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATG
GGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGT
TGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGC
CAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCA
Length of 3' end seq. 517
Connected seq. ID AFH123P
Connected seq. 
>AFH123P.Seq
TGCGGNAATTCCTACGTGNCACTGTTGGCCTACTGGACTTTATTTTTTTTTATCTCTATC
TATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTT
AGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGT
TTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGA
ATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAA
CATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTAT
CGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGC
TATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACT
TTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAA
TGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTT
ACCAG----------AATTCAAAGTTGAAAAATGGTTACGATCTTNACTTCAAAACTAAA
AACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTC
ATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCA
TACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACC
AACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAGGCATGTAATGCTCTTTTATTAAAA
GTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCA
TCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGAT
TTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGA
TTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCA
Length of connected seq. 1122
Full length Seq ID -
Full length Seq. -
Length of full length seq. -