>CHO519 (CHO519Q) /CSM/CH/CHO5-A/CHO519Q.Seq.d/
TTTTTTTTTNGGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAANNNNAAAAAAAAAAAAAAAAAAAA
AAANNNTTTTTTTTTTTTTTTTTTNAAAAAAAAAXXXXXXXXXX

FFXGFFFFFFFFFFFFFFFFFFFFFFKKKKKXXKKKKKKKXXFFFFFXKK---

Frame A:
fffxxfffffffffffffffffffff*kkkkkxxkkkkkkkxffffffxkk---


Frame B:
FFXGFFFFFFFFFFFFFFFFFFFFFFKKKKKXXKKKKKKKXXFFFFFXKK---


Frame C:
ffxxffffffffffffffffffffflkkkkkxxkkkkkkkxxfffffxkk---

 ***** No hits found ******

Lambda     K      H
   -1.00    -1.00    -1.00

[blastall] WARNING:  [000.000]  1124669155.dictycdb.dna: SetUpBlastSearch failed.
[blastall] ERROR:  [000.000]  1124669155.dictycdb.dna: BLASTSetUpSearch: Unable to calculate Karlin-Altschul params, check query sequence

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

 ***** No hits found ******

Lambda     K      H
   0.284    0.106    0.239

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 5,306,232

 psg: 1.06  gvh: 0.27  alm: 0.17  top: 1.27  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.36  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CHO519 is cyt

>CHO519F.Seq
TTTTTTTTTNGGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAANNNNAAAAAAAAAAAAAAAAAAAA
AAANNNTTTTTTTTTTTTTTTTTTNAAAAAAAAANNNNNNNNNN