>CHO517 (CHO517Q) /CSM/CH/CHO5-A/CHO517Q.Seq.d/
XXXXXXXXXXCCTCAGCGGCATGAACCCAATCACCACCATGCGTAGTGATGACAACCATT
TACTCTCTTTCCAATTCCTTTTGGATTGCATGGAATTTAGATCTTCAAAACTTTTAATTC
AATCTGCTCAAACCGTTAGTAAGAATTATAAAAAATCAAAAGAATCATTCACAGCATGGA
ATCAATCTTTAGAGATTTTAATTCATTTGGCTAAAGCTCACACTGAAACTATCATCCTTC
AAAAGTTTATTGAAAAAATTCAATCTGAAAAGGATCTCGAATTGGCTTCCATCCTAAAGA
AACTTTGCCAACTCTATGGTCTTTACATCATTAGAGAGGATTTCGAATTCTTTAGAAATA
GTAACTACTTAAAGAAAGGTAAAGCTAAAGCTATCATCAAATTAATCGGTCAATTATGTT
CTGATCTCTCTCAACAAGCTATGCTTTTAGTTAAAGGTTTTGGTAATGAAGAATACTTAA
TGAATTCACCTTTAGGTATGGAGGATGGTGATCTCTATGAAAATATTTGTAATCAAGTTG
GTGGTCCTT

---LSGMNPITTMRSDDNHLLSFQFLLDCMEFRSSKLLIQSAQTVSKNYKKSKESFTAWN
QSLEILIHLAKAHTETIILQKFIEKIQSEKDLELASILKKLCQLYGLYIIREDFEFFRNS
NYLKKGKAKAIIKLIGQLCSDLSQQAMLLVKGFGNEEYLMNSPLGMEDGDLYENICNQVG
GP

Frame A:
---LSGMNPITTMRSDDNHLLSFQFLLDCMEFRSSKLLIQSAQTVSKNYKKSKESFTAWN
QSLEILIHLAKAHTETIILQKFIEKIQSEKDLELASILKKLCQLYGLYIIREDFEFFRNS
NYLKKGKAKAIIKLIGQLCSDLSQQAMLLVKGFGNEEYLMNSPLGMEDGDLYENICNQVG
GP


Frame B:
---saa*tqsppcvvmttiyslsnsfwiawnldlqnf*fnllkplvriiknqknhsqhgi
nl*rf*fiwlkltlklssfksllkkfnlkrisnwlps*rnfansmvftslerisnsleiv
tt*rkvklklssn*svnyvlislnklcf*lkvlvmknt**ihl*vwrmvismkifviklv
vl


Frame C:
---qrhepnhhha***qpftlfpipfglhgi*ifktfnsicsnr**el*kikriihsmes
ifrdfnsfg*ssh*nyhpskvy*knsi*kgsrigfhpketlptlwslhh*rgfril*k**
llker*s*syhqinrsimf*slstsyafs*rfw**rilneftfryggw*sl*kyl*ssww
s

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHO517 (CHO517Q) /CSM/CH/CHO5-A/CHO517Q.Seq.d/                       1068   0.0
AHO723 (AHO723Q) /CSM/AH/AHO7-A/AHO723Q.Seq.d/                        938   0.0
CHG686 (CHG686Q) /CSM/CH/CHG6-D/CHG686Q.Seq.d/                        900   0.0
SHD580 (SHD580Q) /CSM/SH/SHD5-D/SHD580Q.Seq.d/                        668   0.0
VHF141 (VHF141Q) /CSM/VH/VHF1-B/VHF141Q.Seq.d/                        557   e-158
VFH510 (VFH510Q) /CSM/VF/VFH5-A/VFH510Q.Seq.d/                         40   0.035
SFE583 (SFE583Q) /CSM/SF/SFE5-D/SFE583Q.Seq.d/                         40   0.035
VHJ343 (VHJ343Q) /CSM/VH/VHJ3-B/VHJ343Q.Seq.d/                         38   0.14
VHB348 (VHB348Q) /CSM/VH/VHB3-B/VHB348Q.Seq.d/                         38   0.14
SHK866 (SHK866Q) /CSM/SH/SHK8-C/SHK866Q.Seq.d/                         38   0.14

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          36   0.006  17
AF494278|AF494278.1 Chaetosphaeridium globosum chloroplast, complete genome.    34   0.007  9
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    38   0.016  7
AJ010592|AJ010592.2 Guillardia theta DNA for complete sequence of nucleomorph chromosome 2.    30   0.018  12
AE009951|AE009951.2 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome.    36   0.043  21
AC023072|AC023072.4 Homo sapiens chromosome 11 clone RP11-580I19 map 11, WORKING DRAFT SEQUENCE, 40 unordered pieces.    36   0.075  9
CP000013|CP000013.1 Borrelia garinii PBi, complete genome.         36   0.10   14
AL353696|AL353696.8 Human DNA sequence from clone RP11-560G8 on chromosome 9.    48   0.18   1
AW829533|AW829533.1 ra41c11.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to contains element MER33 repetitive element ;, mRNA sequence.    32   0.51   2
AW830038|AW830038.1 ra48h04.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to contains element MER33 repetitive element ;, mRNA sequence.    32   0.51   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF051203_1(AF051203|pid:none) Picea mariana acyl-CoA oxidase hom...   100   2e-20
U66299_1(U66299|pid:none) Phalaenopsis sp. 'True Lady' acyl-CoA ...    90   3e-17
U77053_1(U77053|pid:none) Phalaenopsis cv. 'True Lady' acyl-CoA ...    90   3e-17
(O64894) RecName: Full=Acyl-coenzyme A oxidase, peroxisomal;    ...    88   1e-16
(O65201) RecName: Full=Acyl-coenzyme A oxidase 2, peroxisomal;  ...    87   2e-16
AF057043_1(AF057043|pid:none) Arabidopsis thaliana acyl-CoA oxid...    87   2e-16
AF202987_1(AF202987|pid:none) Petroselinum crispum acyl-CoA oxid...    82   7e-15
AK317051_1(AK317051|pid:none) Arabidopsis thaliana AT5G65110 mRN...    80   3e-14
AX155021_1(AX155021|pid:none) Sequence 67 from Patent WO0138484.       77   2e-13
(Q15067) RecName: Full=Peroxisomal acyl-coenzyme A oxidase 1;   ...    68   1e-10

 psg: 0.55  gvh: 0.45  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for CHO517 is cyt

>CHO517Z.Seq
NNNNNNNNNNCCTCAGCGGCATGAACCCAATCACCACCATGCGTAGTGATGACAACCATT
TACTCTCTTTCCAATTCCTTTTGGATTGCATGGAATTTAGATCTTCAAAACTTTTAATTC
AATCTGCTCAAACCGTTAGTAAGAATTATAAAAAATCAAAAGAATCATTCACAGCATGGA
ATCAATCTTTAGAGATTTTAATTCATTTGGCTAAAGCTCACACTGAAACTATCATCCTTC
AAAAGTTTATTGAAAAAATTCAATCTGAAAAGGATCTCGAATTGGCTTCCATCCTAAAGA
AACTTTGCCAACTCTATGGTCTTTACATCATTAGAGAGGATTTCGAATTCTTTAGAAATA
GTAACTACTTAAAGAAAGGTAAAGCTAAAGCTATCATCAAATTAATCGGTCAATTATGTT
CTGATCTCTCTCAACAAGCTATGCTTTTAGTTAAAGGTTTTGGTAATGAAGAATACTTAA
TGAATTCACCTTTAGGTATGGAGGATGGTGATCTCTATGAAAATATTTGTAATCAAGTTG
GTGGTCCTT