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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH110
Library AF
(Link to library)
Clone ID AFH110
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15597-1
Original site URL
Representative seq. ID AFH110P
(Link to Original site)
Representative DNA sequence 
>AFH110 (AFH110Q) /CSM/AF/AFH1-A/AFH110Q.Seq.d/
AATAAACTTTTCAAAGTATTATTCGTGATACAGTTAGAGCAAACCTTTTGAAAGCAAATT
CCGATCCTGAAACCATTCATGAACTTTATAGTGAGATTGCATCTGGTTATTCCGATACAC
CAATCATTAGAATCGATTGGTTGAATATTTTAGCAAATAAACATATCGAAGAGGAGAATT
TCTTTGAGGCANCAGTTTGTAAACTTAGAATAGCTCTATTGATCATXXXXXXXXXXGTGT
TAATTGGCCCGCTCATCATATTGAAACTTTGGCTCAGGAATGTACAATCTTTTTAAAACA
TTGTAAACGTGCTTTGGATATCAATAAGAATAAGATTCAAATGAATCAAATCTCTTTTCA
TCAAGAATTGGAATTGGGTTATGCTAAACTTTACTTTAAAATGGATCGTTATATTCGTGC
TGAAATTAATAATGAATCTGATGAAGAAGCTATCGATATAGATCAACAACCTGAAACTGT
TCAATCTTCATATGGAGCCGAGGATGAATCTTCTCTAATCACTGATTTATCATATGATGA
TGATATCTCTATTAAAGAATCTTCACCAAATAATATTTTAGATAATTTATTACCAAATTT
AAAAAAGAAATCAATGGCTAATTTTTTAACAATTAAAGAAGATAAACGTATTAAAAGAGG
TTCTGCTGAATTTGATAATAATTCAACAACATTAAATAATAATAATAGTAATAATAATAA
TAATAATAATTCAAATAGTAATAATAATTCACCATTACCAATCCAAACTCCAAGATCAAN
AANNATAAAAAATCTTCAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
*TFQSIIRDTVRANLLKANSDPETIHELYSEIASGYSDTPIIRIDWLNILANKHIEEENF
FEAXVCKLRIALLI---

---VNWPAHHIETLAQECTIFLKHCKRALDINKNKIQMNQISFHQELELGYAKLYFKMDR
YIRAEINNESDEEAIDIDQQPETVQSSYGAEDESSLITDLSYDDDISIKESSPNNILDNL
LPNLKKKSMANFLTIKEDKRIKRGSAEFDNNSTTLNNNNSNNNNNNNSNSNNNSPLPIQT
PRSXXIKNLQ
Translated Amino Acid sequence (All Frames) 
Frame A:
nklfkvlfviqleqtf*kqipilkpfmnfivrlhlvipihqslesig*if*qiniskrri
slrxqfvnle*ly*s---

---vligpliilklwlrnvqsf*nivnvlwisirirfk*ikslfiknwnwvmlnftlkwi
vifvlklimnlmkklsi*innlklfnlhmeprmnll*sliyhmmmisllknlhqiif*ii
yyqi*krnqwlif*qlkkinvlkevllnliiiqqh*iiiiviiiiiiiqiviiihhyqsk
lqdqxx*kifk

Frame B:
infskyys*ys*skpfeskfrs*nhs*tl**dciwlfrytnh*nrlveyfsk*tyrrgef
l*gxsl*t*nssidh---

---c*larssy*nfgsgmynlfktl*tcfgyq*e*dsnesnlfssrigiglc*tll*ngs
lysc*n***i**rsyryrstt*ncsifiwsrg*ifsnh*fii***yly*riftk*yfr*f
itkfkkeing*ffnn*rr*ty*krfc*i***fnnik***********fk****ftitnpn
skixxxkkss

Frame C:
*TFQSIIRDTVRANLLKANSDPETIHELYSEIASGYSDTPIIRIDWLNILANKHIEEENF
FEAXVCKLRIALLI---

---VNWPAHHIETLAQECTIFLKHCKRALDINKNKIQMNQISFHQELELGYAKLYFKMDR
YIRAEINNESDEEAIDIDQQPETVQSSYGAEDESSLITDLSYDDDISIKESSPNNILDNL
LPNLKKKSMANFLTIKEDKRIKRGSAEFDNNSTTLNNNNSNNNNNNNSNSNNNSPLPIQT
PRSXXIKNLQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH110 (AFH110Q) /CSM/AF/AFH1-A/AFH110Q.Seq.d/                       1306   0.0
CFE516 (CFE516Q) /CSM/CF/CFE5-A/CFE516Q.Seq.d/                        896   0.0
SHE765 (SHE765Q) /CSM/SH/SHE7-C/SHE765Q.Seq.d/                        880   0.0
AHL160 (AHL160Q) /CSM/AH/AHL1-C/AHL160Q.Seq.d/                        880   0.0
AFC222 (AFC222Q) /CSM/AF/AFC2-A/AFC222Q.Seq.d/                         42   0.013
VFC837 (VFC837Q) /CSM/VF/VFC8-B/VFC837Q.Seq.d/                         40   0.051
SSA107 (SSA107Q) /CSM/SS/SSA1-A/SSA107Q.Seq.d/                         40   0.051
SLF127 (SLF127Q) /CSM/SL/SLF1-B/SLF127Q.Seq.d/                         40   0.051
SLE771 (SLE771Q) /CSM/SL/SLE7-C/SLE771Q.Seq.d/                         40   0.051
SLD434 (SLD434Q) /CSM/SL/SLD4-B/SLD434Q.Seq.d/                         40   0.051
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   880   0.0    14
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   4e-06  22
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    38   6e-06  14
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   2e-05  16
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   1e-04  16
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34   3e-04  10
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           32   5e-04  21
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           46   0.001  26
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    32   0.002  13
BX323576|BX323576.7 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEYP-77A12.    38   0.005  10
dna update 2004. 3.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014134_17(AE014134|pid:none) Drosophila melanogaster chromosom...    52   2e-05
BC043470_1(BC043470|pid:none) Mus musculus dedicator of cytokine...    51   4e-05
BC024823_1(BC024823|pid:none) Mus musculus dedicator of cytokine...    50   6e-05
DQ118679_1(DQ118679|pid:none) Homo sapiens dedicator of cytokine...    50   6e-05
AK091479_1(AK091479|pid:none) Homo sapiens cDNA FLJ34160 fis, cl...    50   6e-05
DQ118680_1(DQ118680|pid:none) Homo sapiens dedicator of cytokine...    50   6e-05
AK122554_1(AK122554|pid:none) Mus musculus mRNA for mKIAA1771 pr...    50   6e-05
(Q8R1A4) RecName: Full=Dedicator of cytokinesis protein 7;             50   6e-05
AK055905_1(AK055905|pid:none) Homo sapiens cDNA FLJ31343 fis, cl...    50   6e-05
(Q8VDR9) RecName: Full=Dedicator of cytokinesis protein 6;             50   6e-05
protein update 2009. 4.21
PSORT 

 psg: 0.66  gvh: 0.30  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFH110 is nuc
5' end seq. ID AFH110F
5' end seq. 
>AFH110F.Seq
AATAAACTTTTCAAAGTATTATTCGTGATACAGTTAGAGCAAACCTTTTGAAAGCAAATT
CCGATCCTGAAACCATTCATGAACTTTATAGTGAGATTGCATCTGGTTATTCCGATACAC
CAATCATTAGAATCGATTGGTTGAATATTTTAGCAAATAAACATATCGAAGAGGAGAATT
TCTTTGAGGCANCAGTTTGTAAACTTAGAATAGCTCTATTGATCAT----------
Length of 5' end seq. 226
3' end seq. ID AFH110Z
3' end seq. 
>AFH110Z.Seq
----------GTGTTAATTGGCCCGCTCATCATATTGAAACTTTGGCTCAGGAATGTACA
ATCTTTTTAAAACATTGTAAACGTGCTTTGGATATCAATAAGAATAAGATTCAAATGAAT
CAAATCTCTTTTCATCAAGAATTGGAATTGGGTTATGCTAAACTTTACTTTAAAATGGAT
CGTTATATTCGTGCTGAAATTAATAATGAATCTGATGAAGAAGCTATCGATATAGATCAA
CAACCTGAAACTGTTCAATCTTCATATGGAGCCGAGGATGAATCTTCTCTAATCACTGAT
TTATCATATGATGATGATATCTCTATTAAAGAATCTTCACCAAATAATATTTTAGATAAT
TTATTACCAAATTTAAAAAAGAAATCAATGGCTAATTTTTTAACAATTAAAGAAGATAAA
CGTATTAAAAGAGGTTCTGCTGAATTTGATAATAATTCAACAACATTAAATAATAATAAT
AGTAATAATAATAATAATAATAATTCAAATAGTAATAATAATTCACCATTACCAATCCAA
ACTCCAAGATCAANAANNATAAAAAATCTTCAAA
Length of 3' end seq. 564
Connected seq. ID AFH110P
Connected seq. 
>AFH110P.Seq
AATAAACTTTTCAAAGTATTATTCGTGATACAGTTAGAGCAAACCTTTTGAAAGCAAATT
CCGATCCTGAAACCATTCATGAACTTTATAGTGAGATTGCATCTGGTTATTCCGATACAC
CAATCATTAGAATCGATTGGTTGAATATTTTAGCAAATAAACATATCGAAGAGGAGAATT
TCTTTGAGGCANCAGTTTGTAAACTTAGAATAGCTCTATTGATCAT----------GTGT
TAATTGGCCCGCTCATCATATTGAAACTTTGGCTCAGGAATGTACAATCTTTTTAAAACA
TTGTAAACGTGCTTTGGATATCAATAAGAATAAGATTCAAATGAATCAAATCTCTTTTCA
TCAAGAATTGGAATTGGGTTATGCTAAACTTTACTTTAAAATGGATCGTTATATTCGTGC
TGAAATTAATAATGAATCTGATGAAGAAGCTATCGATATAGATCAACAACCTGAAACTGT
TCAATCTTCATATGGAGCCGAGGATGAATCTTCTCTAATCACTGATTTATCATATGATGA
TGATATCTCTATTAAAGAATCTTCACCAAATAATATTTTAGATAATTTATTACCAAATTT
AAAAAAGAAATCAATGGCTAATTTTTTAACAATTAAAGAAGATAAACGTATTAAAAGAGG
TTCTGCTGAATTTGATAATAATTCAACAACATTAAATAATAATAATAGTAATAATAATAA
TAATAATAATTCAAATAGTAATAATAATTCACCATTACCAATCCAAACTCCAAGATCAAN
AANNATAAAAAATCTTCAAA
Length of connected seq. 790
Full length Seq ID -
Full length Seq. -
Length of full length seq. -