>CHO216 (CHO216Q) /CSM/CH/CHO2-A/CHO216Q.Seq.d/
ATTTTTTCAGAATAATTTGAAATTCAATTTTATTAAATAATAATTTATCTCATTTTATAT
ATTTTTTTTTTTAGTACTACATAACCCACCTNTAAAAAAAAAANTAAAAAAANTAAAAAA
AACCCCNATTATTTATTTATTNATTNATTTNAXXXXXXXXXX

ffqnnlkfnfik**FISFYIFFFLVLHNPPXKKKXKKXKKTPIIYLXIXX---

Frame A:
ifse*feiqfy*iiiylilyifffstt*ptxkkkxkkxkknpxylfixxf---


Frame B:
ffqnnlkfnfik**FISFYIFFFLVLHNPPXKKKXKKXKKTPIIYLXIXX---


Frame C:
ffrii*nsillnnnlshfiyfff*yyithl*kkx*kx*kkpxlfiyxxi---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG668 (SHG668Q) /CSM/SH/SHG6-C/SHG668Q.Seq.d/                         38   0.037
SHD540 (SHD540Q) /CSM/SH/SHD5-B/SHD540Q.Seq.d/                         38   0.037
CHQ423 (CHQ423Q) /CSM/CH/CHQ4-A/CHQ423Q.Seq.d/                         38   0.037
CHO216 (CHO216Q) /CSM/CH/CHO2-A/CHO216Q.Seq.d/                         38   0.037
CHN408 (CHN408Q) /CSM/CH/CHN4-A/CHN408Q.Seq.d/                         38   0.037
CHN319 (CHN319Q) /CSM/CH/CHN3-A/CHN319Q.Seq.d/                         38   0.037
CHL750 (CHL750Q) /CSM/CH/CHL7-C/CHL750Q.Seq.d/                         38   0.037
CHL505 (CHL505Q) /CSM/CH/CHL5-A/CHL505Q.Seq.d/                         38   0.037
CHL488 (CHL488Q) /CSM/CH/CHL4-D/CHL488Q.Seq.d/                         38   0.037
CHL262 (CHL262Q) /CSM/CH/CHL2-C/CHL262Q.Seq.d/                         38   0.037

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    34   0.028  2
BX178728|BX178728.1 Danio rerio genomic clone DKEY-181B17, genomic survey sequence.    34   0.035  2
AC110309|AC110309.5 Rattus norvegicus clone CH230-144A3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    32   0.099  2
AC117137|AC117137.4 Rattus norvegicus clone CH230-385F19, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    32   0.10   2
CR450847|CR450847.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-235J21.    32   0.10   2
CK415321|CK415321.1 AUF_IpPit_32_g04 Pituitary cDNA library Ictalurus punctatus cDNA 5', mRNA sequence.    32   0.13   2
CZ206264|CZ206264.1 AIAA-aac83d14.b1 Ancylostoma caninum whole genome shotgun library (AIAAGSS 001) Ancylostoma caninum genomic, genomic survey sequence.    34   0.13   2
CG726828|CG726828.1 1119091G10.y1 1119 - RescueMu Grid AA Zea mays genomic, genomic survey sequence.    34   0.13   2
CG721744|CG721744.1 1119068G10.x1 1119 - RescueMu Grid AA Zea mays genomic, genomic survey sequence.    34   0.13   2
CG731059|CG731059.1 1119133A07.y1 1119 - RescueMu Grid AA Zea mays genomic, genomic survey sequence.    34   0.14   2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 86,716,131

 psg: 0.99  gvh: 0.41  alm: 0.40  top: 0.97  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal

>> prediction for CHO216 is mit

>CHO216F.Seq
ATTTTTTCAGAATAATTTGAAATTCAATTTTATTAAATAATAATTTATCTCATTTTATAT
ATTTTTTTTTTTAGTACTACATAACCCACCTNTAAAAAAAAAANTAAAAAAANTAAAAAA
AACCCCNATTATTTATTTATTNATTNATTTNANNNNNNNNNN