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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHO182
Library CH
(Link to library)
Clone ID CHO182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHO182P
(Link to Original site)
Representative DNA sequence 
>CHO182 (CHO182Q) /CSM/CH/CHO1-D/CHO182Q.Seq.d/
TAACTAAGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTAT
TTTTTATGATATTCCTTGTATCAAAATCCTATGCNACGGATTGTAATAAAATTACTAATG
AANAAAAATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNG
AACAAAAATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATNTGAAAATAGTTGTT
ATAGAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATG
GATTTATTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAAT
GTGCCTTATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCT
GTTGCCCAGGTGAATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTG
GTTCATCAGXXXXXXXXXXTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGA
CCACCACCAAAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTT
GGTGAAGAATGCTGTGTTAAAAGTAGAAATGATTGTT
sequence update 2002.10.25
Translated Amino Acid sequence 
tklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXKCHKSSECIVINYTPCXGE
QKWACSKGTFDTCTXENSCYRNSSNNQVVEVSNKCFNLDGFIKITTPTEYSCSDAXIKXC
ALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSS---

---DQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEECCVKSRNDC
Translated Amino Acid sequence (All Frames) 
Frame A:
*ls*k*knnkgccf*kileyfl*yslyqnpmxrivikllmxknvinlrnvll*iihhvxx
nknglalkvhlilvhxkivvieihpitkllkyqinasilmdllklqlqpsilvlmpxlxn
vpy*vnhvvskxtlvqiqlpvaqvnqfvkv*alvvqhlvvvhq---

---linmeknvallpidhhqnah*dvhqnmnvesitlvknavlkvemiv

Frame B:
n*vknkkiikdvvskkywsifydipcikilcxgl**ny**xkms*ifgmycyklytmlxx
tkmgll*ryi*ylyx*k*ll*kfiq*psc*sik*mlqs*wiy*nynsnrvflf*cqx*xm
clir*im*fpkxllfksnfllpr*inl*rfklw*fniwwwfi---

---*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rmlc*k*k*l

Frame C:
tklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEXKCHKSSECIVINYTPCXGE
QKWACSKGTFDTCTXENSCYRNSSNNQVVEVSNKCFNLDGFIKITTPTEYSCSDAXIKXC
ALLGKSCSFQXNSCSNPTSCCPGESICEGLSSGSSTSGGGSS---

---DQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEECCVKSRNDC
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG501 (SLG501Q) /CSM/SL/SLG5-A/SLG501Q.Seq.d/                        799   0.0
SHJ685 (SHJ685Q) /CSM/SH/SHJ6-D/SHJ685Q.Seq.d/                        799   0.0
SFL250 (SFL250Q) /CSM/SF/SFL2-C/SFL250Q.Seq.d/                        799   0.0
SFK426 (SFK426Q) /CSM/SF/SFK4-B/SFK426Q.Seq.d/                        799   0.0
SFJ257 (SFJ257Q) /CSM/SF/SFJ2-C/SFJ257Q.Seq.d/                        799   0.0
SFH185 (SFH185Q) /CSM/SF/SFH1-D/SFH185Q.Seq.d/                        799   0.0
SFG607 (SFG607Q) /CSM/SF/SFG6-A/SFG607Q.Seq.d/                        799   0.0
SFE825 (SFE825Q) /CSM/SF/SFE8-B/SFE825Q.Seq.d/                        799   0.0
SFE812 (SFE812Q) /CSM/SF/SFE8-A/SFE812Q.Seq.d/                        799   0.0
SFB513 (SFB513Q) /CSM/SF/SFB5-A/SFB513Q.Seq.d/                        799   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.   490   0.0    6
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.   490   0.0    7
AX348726|AX348726.1 Sequence 184 from Patent WO0202807.            32   0.47   4
AX344319|AX344319.1 Sequence 166 from Patent WO0200926.            32   0.47   4
AX251923|AX251923.1 Sequence 184 from Patent WO0168911.            32   0.47   4
BD460387|BD460387.1 Diagnosis of Diseases Associated with Cell Cycle.    32   0.47   4
BD452309|BD452309.1 Diagnosis of Diseases Associated with Cell Cycle.    32   0.47   4
AC132507|AC132507.3 Rattus norvegicus clone CH230-197J3, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34   0.64   7
AC124167|AC124167.2 Tetraodon nigroviridis clone GSTNB-42J2, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38   0.68   2
AC021039|AC021039.12 Homo sapiens chromosome 10 clone RP11-478K18, complete sequence.    34   1.2    5
dna update 2005. 8.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   218   1e-55
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    95   1e-18
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    56   9e-07
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    52   2e-05
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        49   1e-04
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    45   0.002
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    33   0.005
AF176515_1(AF176515|pid:none) Mus musculus EIG3 protein (Eig3) m...    37   0.59
(Q9BYS1) RecName: Full=Keratin-associated protein 1-5; AltName: ...    33   8.5
AK131904_1(AK131904|pid:none) Mus musculus adult male tongue cDN...    33   8.5
protein update 2009. 4. 9
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.40  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for CHO182 is exc
5' end seq. ID CHO182F
5' end seq. 
>CHO182F.Seq
TAACTAAGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTAT
TTTTTATGATATTCCTTGTATCAAAATCCTATGCNACGGATTGTAATAAAATTACTAATG
AANAAAAATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNG
AACAAAAATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATNTGAAAATAGTTGTT
ATAGAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATG
GATTTATTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAAT
GTGCCTTATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCT
GTTGCCCAGGTGAATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTG
GTTCATCAGNNNNNNNNNN
Length of 5' end seq. 499
3' end seq. ID CHO182Z
3' end seq. 
>CHO182Z.Seq
NNNNNNNNNNTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCA
AAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAA
TGCTGTGTTAAAAGTAGAAATGATTGTT
Length of 3' end seq. 148
Connected seq. ID CHO182P
Connected seq. 
>CHO182P.Seq
TAACTAAGTTAAAAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTAT
TTTTTATGATATTCCTTGTATCAAAATCCTATGCNACGGATTGTAATAAAATTACTAATG
AANAAAAATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNG
AACAAAAATGGGCTTGCTCTAAAGGTACATTTGATACTTGTACATNTGAAAATAGTTGTT
ATAGAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATG
GATTTATTAAAATTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAANATTAAANAAT
GTGCCTTATTAGGTAAATCATGTAGTTTCCAAAANAACTCTTGTTCAAATCCAACTTCCT
GTTGCCCAGGTGAATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAACATCTGGTGGTG
GTTCATCAG----------TTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGA
CCACCACCAAAATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTT
GGTGAAGAATGCTGTGTTAAAAGTAGAAATGATTGTT
Length of connected seq. 627
Full length Seq ID -
Full length Seq. -
Length of full length seq. -