>AFG865 (AFG865Q) /CSM/AF/AFG8-C/AFG865Q.Seq.d/
TCATTATTCAATTTTATTTTTAATNAATTCAAATTAAAAAAAAAAACAATAACTAACTAA
GTTAAAAATAAAAAAATAATAAAGGATGTTGTTTNTAAAAAATATTGGAGTATTTTTTAT
GATATTCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAANA
ATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNGAACAAAA
ATGGGCTTGCTNTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAA
TTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTAT
TAAAATTACAACTCCAACCGAGTNTTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTT
ATTAGGTAAATCATGTAGTTTCCAAAANAACTNTTGTTCAAATCCAACTTCCTGTTGCCC
AGGNGAATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAAXXXXXXXXXX

hysilflxnsn*kkkq*ltklkikk**RMLFXKNIGVFFMIFLVSKSYATDCNKITNEEX
CHKSSECIVINYTPCXGEQKWACXKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFI
KITTPTEXSCSDAKIKEXALLGKSCSFQXNXCSNPTSCCPGESICEGLSSGSS---

Frame A:
slfnfifnxfklkkktitn*vknkkiikdvvxkkywsifydipcikilcdgl**ny**rx
ms*ifgmycyklytmlxxtkmgll*ryi*ylyi*k*ll*kfiq*psc*sik*mlqs*wiy
*nynsnrvflf*cqd*rmxlir*im*fpkxllfksnfllprxinl*rfklw*f---


Frame B:
hysilflxnsn*kkkq*ltklkikk**RMLFXKNIGVFFMIFLVSKSYATDCNKITNEEX
CHKSSECIVINYTPCXGEQKWACXKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFI
KITTPTEXSCSDAKIKEXALLGKSCSFQXNXCSNPTSCCPGESICEGLSSGSS---


Frame C:
iiqfyf*xiqikkknnn*ls*k*knnkgccx*kileyfl*yslyqnpmrrivikllmkxn
vinlrnvll*iihhvxxnknglaxkvhlilvhmkivvieihpitkllkyqinasilmdll
klqlqpsxlvlmprlknxpy*vnhvvskxtxvqiqlpvaqxnqfvkv*alvv---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG501 (SLG501Q) /CSM/SL/SLG5-A/SLG501Q.Seq.d/                        823   0.0
SHJ685 (SHJ685Q) /CSM/SH/SHJ6-D/SHJ685Q.Seq.d/                        823   0.0
SFL250 (SFL250Q) /CSM/SF/SFL2-C/SFL250Q.Seq.d/                        823   0.0
SFK426 (SFK426Q) /CSM/SF/SFK4-B/SFK426Q.Seq.d/                        823   0.0
SFK212 (SFK212Q) /CSM/SF/SFK2-A/SFK212Q.Seq.d/                        823   0.0
SFJ633 (SFJ633Q) /CSM/SF/SFJ6-B/SFJ633Q.Seq.d/                        823   0.0
SFJ257 (SFJ257Q) /CSM/SF/SFJ2-C/SFJ257Q.Seq.d/                        823   0.0
SFI696 (SFI696Q) /CSM/SF/SFI6-D/SFI696Q.Seq.d/                        823   0.0
SFI632 (SFI632Q) /CSM/SF/SFI6-B/SFI632Q.Seq.d/                        823   0.0
SFH201 (SFH201Q) /CSM/SF/SFH2-A/SFH201Q.Seq.d/                        823   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.   630   0.0    5
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.   630   0.0    6
AC133864|AC133864.11 Medicago truncatula clone mth2-18d12, WORKING DRAFT SEQUENCE, 4 ordered pieces.    42   0.005  3
AX348726|AX348726.1 Sequence 184 from Patent WO0202807.            32   0.008  5
AX344319|AX344319.1 Sequence 166 from Patent WO0200926.            32   0.008  5
AX251923|AX251923.1 Sequence 184 from Patent WO0168911.            32   0.008  5
AC079408|AC079408.25 Homo sapiens 12q BAC RP11-601I4 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38   0.077  6
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    36   0.14   10
BX572090|BX572090.1 Prochlorococcus marinus MED4 complete genome; segment 1/5.    34   0.18   9
AE014840|AE014840.1 Plasmodium falciparum 3D7 chromosome 11 section 5 of 8 of the complete sequence.    30   0.20   9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   225   6e-58
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    96   5e-19
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        42   0.009
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    39   0.10
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    35   1.5
(Q99088) RecName: Full=Low-density lipoprotein receptor 2;      ...    34   2.5
QRXLL2(B40388)LDL receptor 2 precursor - African clawed frog           34   2.5
(Q99087) RecName: Full=Low-density lipoprotein receptor 1;      ...    33   3.3
BC170345_1(BC170345|pid:none) Xenopus laevis low density lipopro...    33   3.3
QRXLL1(A40388)LDL receptor 1 precursor - African clawed frog           33   3.3

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.27  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for AFG865 is exc

>AFG865F.Seq
TCATTATTCAATTTTATTTTTAATNAATTCAAATTAAAAAAAAAAACAATAACTAACTAA
GTTAAAAATAAAAAAATAATAAAGGATGTTGTTTNTAAAAAATATTGGAGTATTTTTTAT
GATATTCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAANA
ATGTCATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNGAACAAAA
ATGGGCTTGCTNTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAA
TTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTAT
TAAAATTACAACTCCAACCGAGTNTTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTT
ATTAGGTAAATCATGTAGTTTCCAAAANAACTNTTGTTCAAATCCAACTTCCTGTTGCCC
AGGNGAATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAA----------