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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHN254
Library CH
(Link to library)
Clone ID CHN254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13080-1
Original site URL
Representative seq. ID CHN254P
(Link to Original site)
Representative DNA sequence 
>CHN254 (CHN254Q) /CSM/CH/CHN2-C/CHN254Q.Seq.d/
TGGCCTACTGGAAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATA
TATATTAATATATTAATATTAATATAATGAAAATATTATATTCATTATTATTAATATCAA
GTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGG
CATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAAC
CAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAAT
ATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGG
TAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGAXX
XXXXXXXXAGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCAC
TCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTC
TTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAA
CTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTG
GTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTC
CAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTC
CACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTG
GTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAA
CAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAA
ATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAA
ATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATG
TTTATGTCACTTCTTTTACTTCTTCTGATGCTGGTGGTTATTATTTTAAGGTTGTTCATT
GGTATTATTCCAAAAAAATAAAATAATAAAAAAATATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
gllekiliivynifkyiy*yiliy*y*yneniifiiinikynfkycikyfitsi*styfg
ikrkmlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlfic*wyiyi*WINACP
PGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQP
PTQPPTQPPTQPPTPPPFTGSAVAGLLFTDINGNGIFDG---

---SGGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGGYYFKVVH
WYYSKKIK**kni
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgknfnyci*yf*iyiliyinilili**kyyihyy*yqv*f*ily*ifhhkyminvfw
h*kenvi*iqikmhcmmkvnqdlemlkfl*emqliireiniqyvihllmvhlymmd*rls
trctmyssngfs**fgsrsfsckylcl*hsid*tswcfirliytys*n*c*fglc*anst
tnsttysttysttntstiyw*crrwfiiyryqw*wnf*w---

---SGGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGGYYFKVVH
WYYSKKIK**kni

Frame B:
gllekiliivynifkyiy*yiliy*y*yneniifiiinikynfkycikyfitsi*styfg
ikrkmlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlfic*wyiyi*WINACP
PGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQP
PTQPPTQPPTQPPTPPPFTGSAVAGLLFTDINGNGIFDG---

---vevnvvvllvpeliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvilllq
lvsitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmillf
hnivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklisvq
iiiv*fllhssklvlplq*emliqliqiqivmlsyqllimfmsllllllmlvviilrlfi
giipkk*nnkki*

Frame C:
aywkkf*llyiiflniyiniy*yininimkilyslllissiilntvlnissqvydqrila
lkgkcyldsnknalydegepgfgnveislgnatdykgnkypiryssadgtfiydgltlvh
qvyhvfvqrfqliiwqqif*lqiplpitfh*lnklvfhqahlylqlelvliwvmlsklnh
qlnhllnhllnhqhlhhllvvpslvyylpismvmeflm---

---wr*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yycyn
wfrll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ffss
tiscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fqfk
lllcnsccilpswffhynekc*snsskyk**cflinc**clchffyff*cwwllf*gcsl
vlfqknkiikky
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHN254 (CHN254Q) /CSM/CH/CHN2-C/CHN254Q.Seq.d/                       2444   0.0
SHL110 (SHL110Q) /CSM/SH/SHL1-A/SHL110Q.Seq.d/                       1433   0.0
SHG853 (SHG853Q) /CSM/SH/SHG8-C/SHG853Q.Seq.d/                       1433   0.0
SHG831 (SHG831Q) /CSM/SH/SHG8-B/SHG831Q.Seq.d/                       1433   0.0
SHG581 (SHG581Q) /CSM/SH/SHG5-D/SHG581Q.Seq.d/                       1433   0.0
CHM520 (CHM520Q) /CSM/CH/CHM5-A/CHM520Q.Seq.d/                       1433   0.0
SHL135 (SHL135Q) /CSM/SH/SHL1-B/SHL135Q.Seq.d/                       1431   0.0
SHG417 (SHG417Q) /CSM/SH/SHG4-A/SHG417Q.Seq.d/                       1431   0.0
SHD420 (SHD420Q) /CSM/SH/SHD4-A/SHD420Q.Seq.d/                       1431   0.0
CHG823 (CHG823Q) /CSM/CH/CHG8-A/CHG823Q.Seq.d/                       1431   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    48   5e-07  17
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   3e-05  1
AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   3e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   3e-05  2
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  4
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   5e-04  4
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   5e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   5e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   5e-04  2
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  4
dna update 2005. 7. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    78   6e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    59   4e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05
protein update 2009. 4. 9
PSORT 

 psg: 0.86  gvh: 0.54  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.30  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for CHN254 is cyt
5' end seq. ID CHN254F
5' end seq. 
>CHN254F.Seq
TGGCCTACTGGAAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATA
TATATTAATATATTAATATTAATATAATGAAAATATTATATTCATTATTATTAATATCAA
GTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGG
CATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAAC
CAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAAT
ATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGG
TAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGANN
NNNNNNNN
Length of 5' end seq. 668
3' end seq. ID CHN254Z
3' end seq. 
>CHN254Z.Seq
NNNNNNNNNNAGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATC
ACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGG
TCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTAC
AACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTC
TGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATC
TCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCT
TCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGC
TGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCC
AACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTC
AAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAG
AAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAA
TGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGTGGTTATTATTTTAAGGTTGTTCA
TTGGTATTATTCCAAAAAAATAAAATAATAAAAAAATATAA
Length of 3' end seq. 761
Connected seq. ID CHN254P
Connected seq. 
>CHN254P.Seq
TGGCCTACTGGAAAAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATA
TATATTAATATATTAATATTAATATAATGAAAATATTATATTCATTATTATTAATATCAA
GTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGG
CATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAAC
CAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAAT
ATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGG
TAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGA--
--------AGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCAC
TCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTC
TTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAA
CTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTG
GTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTC
CAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTC
CACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTG
GTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAA
CAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAA
ATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAA
ATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATG
TTTATGTCACTTCTTTTACTTCTTCTGATGCTGGTGGTTATTATTTTAAGGTTGTTCATT
GGTATTATTCCAAAAAAATAAAATAATAAAAAAATATAA
Length of connected seq. 1409
Full length Seq ID -
Full length Seq. -
Length of full length seq. -