>AFG687 (AFG687Q) /CSM/AF/AFG6-D/AFG687Q.Seq.d/
AATAATTAAATAGAGTAAAAAAAAAANGTATATATATATTTAAATTTTATTTAAATGGGT
AGTCCAACTATTAGTGATCAAGAAAAATCCCAAGTTGCCTTTATAGCAGGTAGTAGTTCT
GATATTCATTCAACTACTGTTGCAAGATTGTATGAAGGTAGACAAGGAAGATGGGAATTT
ATGGCCGNCGGTATTGTTTCTGTTGTTTCAAATCGNGTAGATAAAACAAATTATATCAAA
GTTTTCGATTTAAAGGCCAACTACAGAATCATTTTTGAACAAGAAATTTATGACACTTTT
GAATTTCAAAAACAAAGAGATTTCTTTTTTGCATTTGAAGGNGATTCCTGNGTTTATGGC
TTATCATTTGCTGATGTAACTGAAGCAGCTGAATTCTATGGCCAACTTTTAAATTGTAAA
CAAGGAAATATTGGTAAAACTGCTGCAATTAATCCAAATACTTNTTCAACCAAAATTACT
GCATCAACACCAGCACCAAAAGTTGAAAAGAAAAAAGAAAAGAAAGGTTTTATGTCTAAA
TTNTTTTCATCGGAAGAGAAAGATTTGGAAATTTCXXXXXXXXXXAACTTTTNCNATNTG
NTGGTATTAANAAGAGTGAATTAAAGAATNCAGNANNTGNTCAATTCATTGTCAATATCA
TTGGTGANCAAATGGTTGGTGGTTCAGCTCCACCACCAACTCAACCAGGTAGATCAGCTC
CNCCAGCTCCACCATCTTCAAACCAACCAGGTAGATCAGCTCCACCACCACCACCATCTG
TTGGTAAAAGTGCTCCACCACCACCACCACCATCCCATAAGACACCTGCAGCTCCACCAT
CAGGCGGAGGTGCTCCACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCAC
CTCCACCACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCG
GTGGAAGAAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATA
AAACCAATCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTG
ATACTTTAGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAG
ATGATGATGACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATT
CAAAAAAATCATTAAATAAAGATTT

nn*ie*kkxvyiylnfi*MGSPTISDQEKSQVAFIAGSSSDIHSTTVARLYEGRQGRWEF
MAXGIVSVVSNRVDKTNYIKVFDLKANYRIIFEQEIYDTFEFQKQRDFFFAFEGDSXVYG
LSFADVTEAAEFYGQLLNCKQGNIGKTAAINPNTXSTKITASTPAPKVEKKKEKKGFMSK
XFSSEEKDLEI---

---LXXXXGIXKSELKNXXXXQFIVNIIGXQMVGGSAPPPTQPGRSAPPAPPSSNQPGRS
APPPPPSVGKSAPPPPPPSHKTPAAPPSGGGAPPPPPPPPPPSSGPPPPPPPMASAPPSS
GGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLAAAMANRRGG
MREDDEEDDDDDDEWSDY*iil*qnsnskkslnkd

Frame A:
nn*ie*kkxvyiylnfi*MGSPTISDQEKSQVAFIAGSSSDIHSTTVARLYEGRQGRWEF
MAXGIVSVVSNRVDKTNYIKVFDLKANYRIIFEQEIYDTFEFQKQRDFFFAFEGDSXVYG
LSFADVTEAAEFYGQLLNCKQGNIGKTAAINPNTXSTKITASTPAPKVEKKKEKKGFMSK
XFSSEEKDLEI---

---nfxxxxvlxrvn*rxqxxxnslsislvxkwlvvqlhhqlnqvdqlxqlhhlqtnqvd
qlhhhhhllvkvlhhhhhhpirhlqlhhqaevlhhhhhlhhhllvvhhlhhhqwpvllhh
qevvvpaaveemifwlpfvvvqvlrllikpihsqifkvlvmkvhvl*lil*lqlwlivev
v*ekmmkkmmmtmmngqiiklffnkiqiqknh*iki

Frame B:
iik*skkkxyiyi*ilfkwvvqllvikknpklpl*qvvvlifiqlllqdcmkvdkedgnl
wpxvlfllfqix*ikqiiskfsi*rpttesflnkkfmtllnfknkeisflhlkxipxfma
yhllm*lkqlnsmanf*ivnkeilvkllqliqilxqpkllhqhqhqklkrkkkrkvlcln
xfhrkrkiwkf---

---tfxxxwy*xe*ikexxxxsihcqyhw*xngwwfssttnstr*issxsstifkptr*i
sstttticw*kcstttttip*dtcsstirrrcstttttstttf*wsttstttngqcssii
rrwwcqrrwkk*sfgfhs*wcks*gc**nqstprysksw**rftffs*yfscsyg*s*rw
yerr**rr***r**mvrllnysltkfkfkkiik*rf

Frame C:
*lnrvkkkxiyifkfylng*sny**srkipsclysr**f*ysfnycckiv*r*trkmgiy
grrycfccfksxr*nklyqsfrfkgqlqnhf*trnl*hf*isktkrflfci*rxflxlwl
iic*cn*ss*ilwptfkl*trkyw*nccn*skyxfnqnycintstks*kekrkerfyv*i
xfigrerfgnf---

---LXXXXGIXKSELKNXXXXQFIVNIIGXQMVGGSAPPPTQPGRSAPPAPPSSNQPGRS
APPPPPSVGKSAPPPPPPSHKTPAAPPSGGGAPPPPPPPPPPSSGPPPPPPPMASAPPSS
GGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLAAAMANRRGG
MREDDEEDDDDDDEWSDY*iil*qnsnskkslnkd

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSL103 (SSL103Q) /CSM/SS/SSL1-A/SSL103Q.Seq.d/                        902   0.0
AFG687 (AFG687Q) /CSM/AF/AFG6-D/AFG687Q.Seq.d/                        902   0.0
VSJ735 (VSJ735Q) /CSM/VS/VSJ7-B/VSJ735Q.Seq.d/                        757   0.0
CFG349 (CFG349Q) /CSM/CF/CFG3-C/CFG349Q.Seq.d/                        735   0.0
SFG565 (SFG565Q) /CSM/SF/SFG5-C/SFG565Q.Seq.d/                        478   e-133
VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/                        416   e-115
VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/                        416   e-115
VFA863 (VFA863Q) /CSM/VF/VFA8-C/VFA863Q.Seq.d/                        416   e-115
SLH341 (SLH341Q) /CSM/SL/SLH3-B/SLH341Q.Seq.d/                        416   e-115
SHE721 (SHE721Q) /CSM/SH/SHE7-A/SHE721Q.Seq.d/                        416   e-115

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF305060|AF305060.1 Dictyostelium discoideum Wiscott-Aldrich syndrome protein (wasA) gene, complete cds.   442   0.0    10
AC106880|AC106880.4 Homo sapiens BAC clone RP11-709E23 from 4, complete sequence.    40   0.007  7
AC023415|AC023415.3 Homo sapiens chromosome 3 clone RP11-200I19 map 3, WORKING DRAFT SEQUENCE, 27 unordered pieces.    52   0.019  1
AC092933|AC092933.21 Homo sapiens 3 BAC RP11-200I19 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    52   0.019  1
AC131148|AC131148.2 Homo sapiens clone RP11-622J3, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    52   0.019  1
AC117315|AC117315.4 Rattus norvegicus clone CH230-319G22, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces.    44   0.056  9
AC091221|AC091221.3 Drosophila melanogaster 3L BAC RP98-9I19 (Roswell Park Cancer Institute Drosophila BAC Library) complete sequence.    44   0.069  11
AE014847|AE014847.1 Plasmodium falciparum 3D7 chromosome 12, section 4 of 9 of the complete sequence.    36   0.070  10
AL136293|AL136293.5 Human chromosome 14 DNA sequence BAC C-2555C10 of library CalTech-D  from chromosome 14 of Homo sapiens (Human).    50   0.076  1
BX936360|BX936360.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-5I18.    50   0.076  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF305060_1(AF305060|pid:none) Dictyostelium discoideum Wiscott-A...   294   2e-84
DQ985464_1(DQ985464|pid:none) Suberites domuncula Wiskott-Aldric...    74   1e-11
CR380953_413(CR380953|pid:none) Candida glabrata strain CBS138 c...    67   9e-10
CR382137_196(CR382137|pid:none) Debaryomyces hansenii strain CBS...    65   6e-09
(Q12446) RecName: Full=Proline-rich protein LAS17;  &AX167565_1(...    64   8e-09
CP000497_454(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    64   8e-09
AE016820_654(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...    64   1e-08
BC066424_1(BC066424|pid:none) Danio rerio Wiskott-Aldrich syndro...    63   2e-08
AM295156_1(AM295156|pid:none) Homo sapiens mRNA for WASL protein.      62   4e-08
(Q95107) RecName: Full=Neural Wiskott-Aldrich syndrome protein; ...    62   4e-08

 psg: 0.75  gvh: 0.52  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for AFG687 is cyt

>AFG687F.Seq
AATAATTAAATAGAGTAAAAAAAAAANGTATATATATATTTAAATTTTATTTAAATGGGT
AGTCCAACTATTAGTGATCAAGAAAAATCCCAAGTTGCCTTTATAGCAGGTAGTAGTTCT
GATATTCATTCAACTACTGTTGCAAGATTGTATGAAGGTAGACAAGGAAGATGGGAATTT
ATGGCCGNCGGTATTGTTTCTGTTGTTTCAAATCGNGTAGATAAAACAAATTATATCAAA
GTTTTCGATTTAAAGGCCAACTACAGAATCATTTTTGAACAAGAAATTTATGACACTTTT
GAATTTCAAAAACAAAGAGATTTCTTTTTTGCATTTGAAGGNGATTCCTGNGTTTATGGC
TTATCATTTGCTGATGTAACTGAAGCAGCTGAATTCTATGGCCAACTTTTAAATTGTAAA
CAAGGAAATATTGGTAAAACTGCTGCAATTAATCCAAATACTTNTTCAACCAAAATTACT
GCATCAACACCAGCACCAAAAGTTGAAAAGAAAAAAGAAAAGAAAGGTTTTATGTCTAAA
TTNTTTTCATCGGAAGAGAAAGATTTGGAAATTTC----------

>AFG687Z.Seq
----------AACTTTTNCNATNTGNTGGTATTAANAAGAGTGAATTAAAGAATNCAGNA
NNTGNTCAATTCATTGTCAATATCATTGGTGANCAAATGGTTGGTGGTTCAGCTCCACCA
CCAACTCAACCAGGTAGATCAGCTCCNCCAGCTCCACCATCTTCAAACCAACCAGGTAGA
TCAGCTCCACCACCACCACCATCTGTTGGTAAAAGTGCTCCACCACCACCACCACCATCC
CATAAGACACCTGCAGCTCCACCATCAGGCGGAGGTGCTCCACCACCACCACCACCTCCA
CCACCACCTTCTAGTGGTCCACCACCTCCACCACCACCAATGGCCAGTGCTCCTCCATCA
TCAGGAGGTGGTGGTGCCAGCGGCGGTGGAAGAAATGATCTTTTGGCTTCCATTCGTAGT
GGTGCAAGTCTTAAGGCTGTTGATAAAACCAATCCACTCCCAGATATTCAAAGTCTTGGT
AATGAAGGTTCACGTTCTTTAGCTGATACTTTAGCTGCAGCTATGGCTAATCGTAGAGGT
GGTATGAGAGAAGATGATGAAGAAGATGATGATGACGATGATGAATGGTCAGATTATTAA
ATTATTCTTTAACAAAATTCAAATTCAAAAAAATCATTAAATAAAGATTT

>AFG687P.Seq
AATAATTAAATAGAGTAAAAAAAAAANGTATATATATATTTAAATTTTATTTAAATGGGT
AGTCCAACTATTAGTGATCAAGAAAAATCCCAAGTTGCCTTTATAGCAGGTAGTAGTTCT
GATATTCATTCAACTACTGTTGCAAGATTGTATGAAGGTAGACAAGGAAGATGGGAATTT
ATGGCCGNCGGTATTGTTTCTGTTGTTTCAAATCGNGTAGATAAAACAAATTATATCAAA
GTTTTCGATTTAAAGGCCAACTACAGAATCATTTTTGAACAAGAAATTTATGACACTTTT
GAATTTCAAAAACAAAGAGATTTCTTTTTTGCATTTGAAGGNGATTCCTGNGTTTATGGC
TTATCATTTGCTGATGTAACTGAAGCAGCTGAATTCTATGGCCAACTTTTAAATTGTAAA
CAAGGAAATATTGGTAAAACTGCTGCAATTAATCCAAATACTTNTTCAACCAAAATTACT
GCATCAACACCAGCACCAAAAGTTGAAAAGAAAAAAGAAAAGAAAGGTTTTATGTCTAAA
TTNTTTTCATCGGAAGAGAAAGATTTGGAAATTTC----------AACTTTTNCNATNTG
NTGGTATTAANAAGAGTGAATTAAAGAATNCAGNANNTGNTCAATTCATTGTCAATATCA
TTGGTGANCAAATGGTTGGTGGTTCAGCTCCACCACCAACTCAACCAGGTAGATCAGCTC
CNCCAGCTCCACCATCTTCAAACCAACCAGGTAGATCAGCTCCACCACCACCACCATCTG
TTGGTAAAAGTGCTCCACCACCACCACCACCATCCCATAAGACACCTGCAGCTCCACCAT
CAGGCGGAGGTGCTCCACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCAC
CTCCACCACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCG
GTGGAAGAAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATA
AAACCAATCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTG
ATACTTTAGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAG
ATGATGATGACGATGATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATT
CAAAAAAATCATTAAATAAAGATTT