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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHM254
Library CH
(Link to library)
Clone ID CHM254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHM254P
(Link to Original site)
Representative DNA sequence 
>CHM254 (CHM254Q) /CSM/CH/CHM2-C/CHM254Q.Seq.d/
TATTATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATG
ATCAACGTATTTTGGCATTAAAAGGAAAATGTTATTTACATTCAAATAAAAATGCATTGT
ATGATGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTANGAAATGCAACTGATT
ATAAGGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATG
GATTAACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTG
AAACCTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTT
CAGCTGATAATTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCAT
TGATTAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTG
ATTTGGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTC
AACCACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATA
TCAATGGTAATGGAATTTTTGATXXXXXXXXXXAGGTAAATGTGGTAGTCTTGTTGGTGC
CGGAGTTGATCAAAAATCACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTT
TGATAATCATAAAACAGGTCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGG
TCGTGATATTATTGTTACAACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCG
TTGGTATAATGCAACCTCTGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTC
AAATGGTCGTACCCCATCTCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTC
AGTGTATGATTCTTCTCTTCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGG
TAATGGTATTCAAGATGCTGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTA
TGGTCAACAATCAACTCCAACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATT
TGAAGTTGATTTCAGTTCAAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGG
TTCTTCCATTACAATGAGAAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTT
CTTATCAACTGTTGATAATGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTAT
TATTTTAATGGTTGTTCATTGGTATTATTCAAAAAAAATAAAATAATAAAAAAATATAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LLISSIILNTVLNISSQVYDQRILALKGKCYLHSNKNALYDEGEPGFGNVEISLXNATDY
KGNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVS
ADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQ
PPTPPPFTGSAVAGLLFTDINGNGIFD---

---GKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTG
FDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQ
YRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNY
YCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVHWY
YSKKIK**kni
Translated Amino Acid sequence (All Frames) 
Frame A:
yy*yqv*f*ily*ifhhkyminvfwh*kenviyiqikmhcmmkvnqdlemlkflxemqli
ireiniqyvihllmvhlymmd*lnvqlmnlhlpiqkiitlkpmkrhwlvhqvyhvfvqrf
qliiwqqif*lqiplpitfh*lnklvfhqahlylqlelvliwvmlsklnhqlnhllnhll
nhqhlhhllvvpslvyylpismvmefl---

---r*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yycynw
frll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ffsst
iscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fqfkl
llcnsccilpswffhynekc*snsskyk**cflinc**clchffyff*cwgyyfngcslv
lfkknkiikkyk

Frame B:
iinikynfkycikyfitsi*styfgikrkmlftfk*kciv**r*triwkc*nffxkcn*l
*gk*isntlfic*wyiyi*winstynl*iyiyqskrlll*nl*kdiglstrctmyssngf
s**fgsrsfsckylcl*hsid*tswcfirliytys*n*c*fglc*ansttnsttysttys
ttntstiyw*crrwfiiyryqw*wnf*---

---GKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTG
FDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQ
YRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNY
YCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVHWY
YSKKIK**kni

Frame C:
LLISSIILNTVLNISSQVYDQRILALKGKCYLHSNKNALYDEGEPGFGNVEISLXNATDY
KGNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVS
ADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQ
PPTPPPFTGSAVAGLLFTDINGNGIFD---

---vnvvvllvpeliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvilllqlv
sitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmillfhn
ivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklisvqii
iv*fllhssklvlplq*emliqliqiqivmlsyqllimfmsllllllmlgllf*wlfigi
iqkk*nnkki*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL582 (SHL582Q) /CSM/SH/SHL5-D/SHL582Q.Seq.d/                       1421   0.0
SHL103 (SHL103Q) /CSM/SH/SHL1-A/SHL103Q.Seq.d/                       1421   0.0
SHK816 (SHK816Q) /CSM/SH/SHK8-A/SHK816Q.Seq.d/                       1421   0.0
SHG836 (SHG836Q) /CSM/SH/SHG8-B/SHG836Q.Seq.d/                       1421   0.0
SHD836 (SHD836Q) /CSM/SH/SHD8-B/SHD836Q.Seq.d/                       1421   0.0
SHD494 (SHD494Q) /CSM/SH/SHD4-D/SHD494Q.Seq.d/                       1421   0.0
SHD486 (SHD486Q) /CSM/SH/SHD4-D/SHD486Q.Seq.d/                       1421   0.0
SHD279 (SHD279Q) /CSM/SH/SHD2-D/SHD279Q.Seq.d/                       1421   0.0
SHC823 (SHC823Q) /CSM/SH/SHC8-A/SHC823Q.Seq.d/                       1421   0.0
SHC405 (SHC405Q) /CSM/SH/SHC4-A/SHC405Q.Seq.d/                       1421   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    48   1e-08  18
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   3e-05  1
AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   3e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   3e-05  2
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   3e-05  4
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   4e-04  4
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   4e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   4e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   4e-04  2
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  4
dna update 2005. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    78   8e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    61   1e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05
protein update 2009. 4. 8
PSORT 

 psg: 0.95  gvh: 0.46  alm: 0.34  top: 0.63  tms: 0.00  mit: 0.31  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for CHM254 is cyt
5' end seq. ID CHM254F
5' end seq. 
>CHM254F.Seq
TATTATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATG
ATCAACGTATTTTGGCATTAAAAGGAAAATGTTATTTACATTCAAATAAAAATGCATTGT
ATGATGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTANGAAATGCAACTGATT
ATAAGGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATG
GATTAACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTG
AAACCTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTT
CAGCTGATAATTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCAT
TGATTAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTG
ATTTGGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTC
AACCACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATA
TCAATGGTAATGGAATTTTTGATNNNNNNNNNN
Length of 5' end seq. 633
3' end seq. ID CHM254Z
3' end seq. 
>CHM254Z.Seq
NNNNNNNNNNAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTCA
CTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTC
ATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTG
GTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTA
AACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAA
CCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCAC
AATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTG
AACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAA
GTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATT
ATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATG
CTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTT
ATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGGT
ATTATTCAAAAAAAATAAAATAATAAAAAAATATAAA
Length of 3' end seq. 757
Connected seq. ID CHM254P
Connected seq. 
>CHM254P.Seq
TATTATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATG
ATCAACGTATTTTGGCATTAAAAGGAAAATGTTATTTACATTCAAATAAAAATGCATTGT
ATGATGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTANGAAATGCAACTGATT
ATAAGGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATG
GATTAACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTG
AAACCTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTT
CAGCTGATAATTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCAT
TGATTAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTG
ATTTGGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTC
AACCACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATA
TCAATGGTAATGGAATTTTTGAT----------AGGTAAATGTGGTAGTCTTGTTGGTGC
CGGAGTTGATCAAAAATCACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTT
TGATAATCATAAAACAGGTCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGG
TCGTGATATTATTGTTACAACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCG
TTGGTATAATGCAACCTCTGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTC
AAATGGTCGTACCCCATCTCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTC
AGTGTATGATTCTTCTCTTCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGG
TAATGGTATTCAAGATGCTGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTA
TGGTCAACAATCAACTCCAACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATT
TGAAGTTGATTTCAGTTCAAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGG
TTCTTCCATTACAATGAGAAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTT
CTTATCAACTGTTGATAATGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTAT
TATTTTAATGGTTGTTCATTGGTATTATTCAAAAAAAATAAAATAATAAAAAAATATAAA
Length of connected seq. 1370
Full length Seq ID -
Full length Seq. -
Length of full length seq. -