>AFG591 (AFG591Q) /CSM/AF/AFG5-D/AFG591Q.Seq.d/
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAAXXXX
XXXXXXAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACA
AATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGA
CCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGA
AGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCAT
CGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAA
AGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTA
CCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCAT
CTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGAC
CTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGC
TGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAGAGAAGC
CGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGA
CAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCAGACGAACTTTAAANAAATA
A

hiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKE
DGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKV
DCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKV
ETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWE---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEREAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIRRTLXK
*

Frame A:
iifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigik
kmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkk
livvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstk
lkpin*lintlwsfhqitptkyssitkrskqvtslmig---

---rdpsr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*s
rscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*t
lpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrq
s*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinqksdqtnfkx
i

Frame B:
syl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*r
rw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks
*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs
*nrsinssihfghftr*hlqstrr*qrdpsr*pr**lg---

---EIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDP
EAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNEL
YLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAK
AEERKKADEKLAAEKAAEREAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVIRRTLXK
*

Frame C:
hiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKE
DGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKV
DCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKV
ETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWE---

---rskqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliq
klsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmns
tslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppk
lkrerkltkn*lpkkllkekpkklmkkkkkslkki*lklmtkkkksknqpkk*sdel*xn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG591 (AFG591Q) /CSM/AF/AFG5-D/AFG591Q.Seq.d/                       2415   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1300   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1300   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1300   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1300   0.0
AFJ522 (AFJ522Q) /CSM/AF/AFJ5-A/AFJ522Q.Seq.d/                       1300   0.0
AFG840 (AFG840Q) /CSM/AF/AFG8-B/AFG840Q.Seq.d/                       1300   0.0
AFE866 (AFE866Q) /CSM/AF/AFE8-C/AFE866Q.Seq.d/                       1300   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       1300   0.0
AFD458 (AFD458Q) /CSM/AF/AFD4-C/AFD458Q.Seq.d/                       1300   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1277   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   1e-27  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-10  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
AL750674|AL750674.1 Pinus pinaster root EST, clone RN06A11 similar to CALRETICULIN.    46   1e-09  4
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   5e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   5e-09  3
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   6e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   6e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   384   e-105
AY395247_1(AY395247|pid:none) Amblyomma brasiliense calreticulin...   211   e-101
AY395246_1(AY395246|pid:none) Amblyomma americanum calreticulin ...   209   e-100
U07708_1(U07708|pid:none) Amblyomma americanum calreticulin (crt...   208   e-100
AY395250_1(AY395250|pid:none) Amblyomma maculatum calreticulin g...   207   3e-99
AY395252_1(AY395252|pid:none) Amblyomma scutatum calreticulin ge...   205   7e-99
AY271304_1(AY271304|pid:none) Haemaphysalis longicornis calretic...   204   1e-97
AF052978_1(AF052978|pid:none) Dirofilaria immitis calreticulin p...   191   1e-97
AY395261_1(AY395261|pid:none) Haemaphysalis leporispalustris cal...   196   2e-96
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   242   2e-62

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFG591 is exc

>AFG591F.Seq
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAA----
------

>AFG591Z.Seq
----------AGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAA
AACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCG
ATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATC
CAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAA
CCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAAT
ACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAAC
TCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTT
CCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAA
AGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCA
AAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAGAG
AAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTG
ATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCAGACGAACTTTAAANA
AATAA

>AFG591P.Seq
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAA----
------AGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACA
AATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGA
CCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGA
AGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCAT
CGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAA
AGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTA
CCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCAT
CTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGAC
CTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGC
TGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAGAGAAGC
CGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGA
CAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTGATCAGACGAACTTTAAANAAATA
A