>AFA152 (AFA152Q) /CSM/AF/AFA1-C/AFA152Q.Seq.d/
XXXXXXXXXXAGTAGTGCTCCATCCTACTGCTTCAAGTAGCGCTCCATCCNCTGCCTCAA
GTAGTGCTACCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGNCNCAGA
AAGANCAGAAAGCTCATCAGCCA

---VVLHPTASSSAPSXASSSATIIISFKFSCIISCKXQKXQKAHQP

Frame A:
---VVLHPTASSSAPSXASSSATIIISFKFSCIISCKXQKXQKAHQP


Frame B:
---*csilllqvalhplpqvvlpsssasssaassaaxxrkxrklis


Frame C:
---sapsycfk*rsixclk*cyhhhqlqvqlhhqlqxxerxesssa

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA152 (AFA152Q) /CSM/AF/AFA1-C/AFA152Q.Seq.d/                        240   4e-63
SFK592 (SFK592Q) /CSM/SF/SFK5-D/SFK592Q.Seq.d/                        200   4e-51
SFJ601 (SFJ601Q) /CSM/SF/SFJ6-A/SFJ601Q.Seq.d/                        200   4e-51
SFI786 (SFI786Q) /CSM/SF/SFI7-D/SFI786Q.Seq.d/                        200   4e-51
SFI514 (SFI514Q) /CSM/SF/SFI5-A/SFI514Q.Seq.d/                        200   4e-51
CHB243 (CHB243Q) /CSM/CH/CHB2-B/CHB243Q.Seq.d/                        200   4e-51
CFC483 (CFC483Q) /CSM/CF/CFC4-D/CFC483Q.Seq.d/                        200   4e-51
AFM763 (AFM763Q) /CSM/AF/AFM7-C/AFM763Q.Seq.d/                        200   4e-51
AFM577 (AFM577Q) /CSM/AF/AFM5-D/AFM577Q.Seq.d/                        200   4e-51
AFM556 (AFM556Q) /CSM/AF/AFM5-C/AFM556Q.Seq.d/                        200   4e-51

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.    88  4e-38  4
BY320491|BY320491.1 Mus musculus synovial fibroblasts cDNA, RIKEN full-length enriched library, clone:L030001I10, 5' end partial sequence.    42  1.6  1
BY182490|BY182490.1 Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630102F15, 5' end partial sequence.    42  1.6  1
BG077191|BG077191.1 H3012A12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3012A12 5', mRNA sequence.    42  1.6  1
BE030910|BE030910.1 129127 MARC 1PIG Sus scrofa cDNA 5', mRNA sequence.    42  1.6  1
AA682117|AA682117.1 vu13a07.r1 Barstead mouse myotubes MPLRB5 Mus musculus cDNA clone IMAGE:1180500 5', mRNA sequence.    42  1.6  1
BY725935|BY725935.1 Mus musculus 2 cells egg cDNA, RIKEN full-length enriched library, clone:B020023O15, 5' end partial sequence.    42  1.6  1
BQ604465|BQ604465.1 MI-P-CP1-nzc-a-05-0-UI.s1 MI-P-CP1 Sus scrofa cDNA clone MI-P-CP1-nzc-a-05-0-UI 3', mRNA sequence.    42  1.6  1
BY189497|BY189497.1 Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630305B15, 5' end partial sequence.    42  1.6  1
BY742063|BY742063.1 Mus musculus 2 days neonate sympathetic ganglion cDNA, RIKEN full-length enriched library, clone:7120491N23, 5' end partial sequence.    42  1.6  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 68,265,014

 psg: 0.93  gvh: 0.55  alm: 0.36  top: 0.70  tms: 0.00  mit: 0.50  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: extracellular, including cell wall

>> prediction for AFA152 is mit

>AFA152Z.Seq
----------AGTAGTGCTCCATCCTACTGCTTCAAGTAGCGCTCCATCCNCTGCCTCAA
GTAGTGCTACCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGNCNCAGA
AAGANCAGAAAGCTCATCAGCCA