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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHL445
Library CH
(Link to library)
Clone ID CHL445
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15897-1
Original site URL
Representative seq. ID CHL445P
(Link to Original site)
Representative DNA sequence 
>CHL445 (CHL445Q) /CSM/CH/CHL4-B/CHL445Q.Seq.d/
AAAACATAAAAGAAAAAGAATCGTAAAGAGGTTAACACTGCACCATTAATCAATAATATT
CGCTTATTATTAAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGANAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTNTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTXXXXXXXXXXTCCAAGAATTTTACCA
CCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTTATCTCCCAGGTAGTGAAGGT
GGTAAACCAATCGCCAACATTTTCATGGGTAATGTAAATCCATCTGGTCGTNTTCCTTTA
ACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAAT
GGTGTCACCACTCCACTNTTCCAATTTGGTGATGGTCTTTNTTATACCACTTTTAACTAT
ACCAATNTCGCCTGTAGCAATTGTAAACCAATCTNTGGTCAATNTGGTAACTACACTGGT
GTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGT
AAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCANAAGTTAAA
ATGTTAANAGGTTTCCAAAAAGTTGATNTTATGCCAGCTAAATCTCAACAAATCTCTTTT
ACCCNCAATGCCTATGAATTCTCCNTTATTGGTGTCGATAATAAAATTACTTTAGAATCG
GTCAATTCATCATTANGGTTGGTAATCAACAATTAGGTTTATATTTACAATAAAAAAAAA
AATTAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
nikekes*rg*hctinq*YSLIIKYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTY
GSSIRVSIVGGEEAEVIEKPRTFGNKRXLKLEYSQIYPKKQLNQENINFMSARDTXVDNL
MSKMSITEKIGQMTQLDITTLTSPNTITINETTLAYYAKTYYIGSYLNSP---

---PRILPPDLVYSCAAVLMAYLPGSEGGKPIANIFMGNVNPSGRXPLTYPGTTGDIGVP
YYHKYSENGVTTPLFQFGDGLXYTTFNYTNXACSNCKPIXGQXGNYTGVLGQSYTFTVTV
TNNGNVQGKDSVLLYLSDLWAQVTPXVKMLXGFQKVDXMPAKSQQISFTXNAYEFSXIGV
DNKITLESVNSSLXLVINN*vyiynkkkn*k
Translated Amino Acid sequence (All Frames) 
Frame A:
kt*kkknrkevntaplinnirlllni*lnsilyiyikr*kllkvyfychc***iy**vqh
mevqle*allevkklkslknhvlleikxnsn*sivkfiqrnn*ikkilisclpeilxlii
*cqkcqslkklvk*lnwilqh*hhqiqlqlmkqh*himlkliildhi*ihq---

---sknfttrsrlqlccsfnglspr**rw*tnrqhfhg*cksiwsxsfnlsryyw*yrcs
ilpqiq*kwchhstxpiw*wsxlyhf*lyqxrl*ql*tnlwsxw*lhwcfrsilyfhchc
hkqw*rsr*rlcslisl*slgssysxs*nvxrfpks*xyas*istnlfypqcl*ilxywc
r**nyfrigqfiixvgnqqlglylq*kkklk

Frame B:
khkrkrivkrltlhh*siifayy*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfni
wkfn*skhcwr*rs*sh*kttyfwk*kxtqirv*snlsketiksrky*fhvcqryxc**f
nvknvnh*knwsndsigyyninitkynyn**nnisilc*nllywiifkfts---

---PRILPPDLVYSCAAVLMAYLPGSEGGKPIANIFMGNVNPSGRXPLTYPGTTGDIGVP
YYHKYSENGVTTPLFQFGDGLXYTTFNYTNXACSNCKPIXGQXGNYTGVLGQSYTFTVTV
TNNGNVQGKDSVLLYLSDLWAQVTPXVKMLXGFQKVDXMPAKSQQISFTXNAYEFSXIGV
DNKITLESVNSSLXLVINN*vyiynkkkn*k

Frame C:
nikekes*rg*hctinq*YSLIIKYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTY
GSSIRVSIVGGEEAEVIEKPRTFGNKRXLKLEYSQIYPKKQLNQENINFMSARDTXVDNL
MSKMSITEKIGQMTQLDITTLTSPNTITINETTLAYYAKTYYIGSYLNSP---

---qefyhqisstvvlqf*wlisqvvkvvnqsptfswvm*ihlvvfl*liqvllvisvfh
ittntvkmvsplhxsnlvmvfxiplltipxspvaivnqsxvnxvttlvf*vnpilslsls
qtmvtfkvktlfsyislifglkllxklkc*xvskklxlcqlnlnksllpxmpmnspllvs
iikll*nrsihhxgw*stirfiftikkkikk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHL445 (CHL445Q) /CSM/CH/CHL4-B/CHL445Q.Seq.d/                       1885   0.0
VHJ645 (VHJ645Q) /CSM/VH/VHJ6-B/VHJ645Q.Seq.d/                       1110   0.0
CFJ426 (CFJ426Q) /CSM/CF/CFJ4-B/CFJ426Q.Seq.d/                       1092   0.0
VHD430 (VHD430Q) /CSM/VH/VHD4-B/VHD430Q.Seq.d/                       1088   0.0
VHJ687 (VHJ687Q) /CSM/VH/VHJ6-D/VHJ687Q.Seq.d/                       1080   0.0
VHH715 (VHH715Q) /CSM/VH/VHH7-A/VHH715Q.Seq.d/                       1078   0.0
VHJ550 (VHJ550Q) /CSM/VH/VHJ5-C/VHJ550Q.Seq.d/                       1074   0.0
VHD344 (VHD344Q) /CSM/VH/VHD3-B/VHD344Q.Seq.d/                       1074   0.0
VHC403 (VHC403Q) /CSM/VH/VHC4-A/VHC403Q.Seq.d/                       1074   0.0
CHR280 (CHR280Q) /CSM/CH/CHR2-D/CHR280Q.Seq.d/                       1074   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.   793   0.0    5
AE014017|AE014017.1 Buchnera aphidicola str. Bp (Baizongia pistaciae), section 2 of 2 of the complete genome.    38   1e-04  16
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    44   1e-04  14
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   3e-04  18
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   3e-04  23
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   5e-04  18
BA000016|BA000016.3 Clostridium perfringens str. 13 DNA, complete genome.    32   6e-04  28
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    40   0.001  14
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    36   0.001  16
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    38   0.001  12
dna update 2005. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   330   4e-89
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   106   1e-21
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...    97   8e-19
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...    92   3e-17
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...    91   7e-17
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...    91   9e-17
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...    89   4e-16
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...    89   4e-16
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...    87   1e-15
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...    86   3e-15
protein update 2009. 4. 8
PSORT 

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.40  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.91  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: Golgi
	 16.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane

>> prediction for CHL445 is cyt
5' end seq. ID CHL445F
5' end seq. 
>CHL445F.Seq
AAAACATAAAAGAAAAAGAATCGTAAAGAGGTTAACACTGCACCATTAATCAATAATATT
CGCTTATTATTAAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGANAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTNTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTNNNNNNNNNN
Length of 5' end seq. 524
3' end seq. ID CHL445Z
3' end seq. 
>CHL445Z.Seq
NNNNNNNNNNTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAA
TGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTTTCATGGGTAATG
TAAATCCATCTGGTCGTNTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTC
CATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTNTTCCAATTTGGTGATG
GTCTTTNTTATACCACTTTTAACTATACCAATNTCGCCTGTAGCAATTGTAAACCAATCT
NTGGTCAATNTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTG
TCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTT
GGGCTCAAGTTACTCCANAAGTTAAAATGTTAANAGGTTTCCAAAAAGTTGATNTTATGC
CAGCTAAATCTCAACAAATCTCTTTTACCCNCAATGCCTATGAATTCTCCNTTATTGGTG
TCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTANGGTTGGTAATCAACAATT
AGGTTTATATTTACAATAAAAAAAAAAATTAAAAAA
Length of 3' end seq. 636
Connected seq. ID CHL445P
Connected seq. 
>CHL445P.Seq
AAAACATAAAAGAAAAAGAATCGTAAAGAGGTTAACACTGCACCATTAATCAATAATATT
CGCTTATTATTAAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGANAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTNTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGT----------TCCAAGAATTTTACCA
CCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTTATCTCCCAGGTAGTGAAGGT
GGTAAACCAATCGCCAACATTTTCATGGGTAATGTAAATCCATCTGGTCGTNTTCCTTTA
ACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAAT
GGTGTCACCACTCCACTNTTCCAATTTGGTGATGGTCTTTNTTATACCACTTTTAACTAT
ACCAATNTCGCCTGTAGCAATTGTAAACCAATCTNTGGTCAATNTGGTAACTACACTGGT
GTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGT
AAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCANAAGTTAAA
ATGTTAANAGGTTTCCAAAAAGTTGATNTTATGCCAGCTAAATCTCAACAAATCTCTTTT
ACCCNCAATGCCTATGAATTCTCCNTTATTGGTGTCGATAATAAAATTACTTTAGAATCG
GTCAATTCATCATTANGGTTGGTAATCAACAATTAGGTTTATATTTACAATAAAAAAAAA
AATTAAAAAA
Length of connected seq. 1140
Full length Seq ID -
Full length Seq. -
Length of full length seq. -