>AFG486 (AFG486Q) /CSM/AF/AFG4-D/AFG486Q.Seq.d/
XXXXXXXXXXTTTACAATTAGCATCTGAAGGTGCACTCTCACTCAAAGAGATTAAACAAT
TCATTCTTGATGAATGTGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTACAAA
AGATCTTCAAACTCATCCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCTCTG
ATACCATTCGTCCAATCTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCAATG
ATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAGAAC
AAAAGAATAAAAAACTCATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTATTT
TCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTTTCC
CATCCATCTGTATTCATCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTAAAT
TCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCGATA
TTGAACGTGTCAATGTCGTCATCAACTACGATATGGCTGAATCTGCC

---LQLASEGALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLSD
TIRPICKKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVIF
VKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGIDI
ERVNVVINYDMAESA

Frame A:
---LQLASEGALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLSD
TIRPICKKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVIF
VKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGIDI
ERVNVVINYDMAESA


Frame B:
---yn*hlkvhshskrlnnsflmnvipcsnhwi*ermykrssnssqptnkl*cfqlpsli
pfvqsvrsl*tihwkstsmmvvnslsmvfnnttyqslknkriknslnswih*tlikplfs
snlldvlmp*irsskilvshpsvfivtsikrivsnntvnsktlnlvswlplislvvvsil
nvsmsssttiwlnl


Frame C:
---tisi*rctltqrd*tihs**m*yhariigyekgctkdlqthpsqqtsydvfsypl*y
hssnl*evyeqsignlhq*w**thspwsstilrtnh*rtke*kth*tlgfirl*ssryfr
qic*tc*clk*dpprywfpihlyss*prskgsyrtip*iqkl*isyhgch*ylwswyry*
tcqcrhqlryg*ic

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE359 (VFE359Q) /CSM/VF/VFE3-C/VFE359Q.Seq.d/                       1144   0.0
FC-AI06 (FC-AI06Q) /CSM/FC/FC-AI/FC-AI06Q.Seq.d/                     1144   0.0
AFO646 (AFO646Q) /CSM/AF/AFO6-B/AFO646Q.Seq.d/                       1144   0.0
AFN644 (AFN644Q) /CSM/AF/AFN6-B/AFN644Q.Seq.d/                       1144   0.0
AFM640 (AFM640Q) /CSM/AF/AFM6-B/AFM640Q.Seq.d/                       1144   0.0
AFL501 (AFL501Q) /CSM/AF/AFL5-A/AFL501Q.Seq.d/                       1144   0.0
AFG486 (AFG486Q) /CSM/AF/AFG4-D/AFG486Q.Seq.d/                       1144   0.0
AFD666 (AFD666Q) /CSM/AF/AFD6-C/AFD666Q.Seq.d/                       1144   0.0
VFA442 (VFA442Q) /CSM/VF/VFA4-B/VFA442Q.Seq.d/                       1142   0.0
AFG357 (AFG357Q) /CSM/AF/AFG3-C/AFG357Q.Seq.d/                       1142   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AW759959|AW759959.1 sl56c07.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-5317 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56   9e-10  2
BF071471|BF071471.1 st59b09.y1 Gm-c1053 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1053-857 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56   9e-10  2
AL408654|AL408654.1 T7 end of clone AV0AA009H09 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    38   2e-09  4
AX488925|AX488925.1 Sequence 6225 from Patent WO02053728.          40   2e-09  5
CN208744|CN208744.1 Tor9242 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    74   3e-09  1
CA251168|CA251168.1 SCQSFL1122D07.g FL1 Saccharum officinarum cDNA clone SCQSFL1122D07 5', mRNA sequence.    48   9e-09  3
CA169742|CA169742.1 SCQGSB1066A04.g SB1 Saccharum officinarum cDNA clone SCQGSB1066A04 5', mRNA sequence.    48   1e-08  3
BI974435|BI974435.1 saj01e10.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-9068 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    52   1e-08  2
CA201831|CA201831.1 SCRLFL1008H03.g FL1 Saccharum officinarum cDNA clone SCRLFL1008H03 5', mRNA sequence.    48   1e-08  3
CA252463|CA252463.1 SCVPFL1138E12.g FL1 Saccharum officinarum cDNA clone SCVPFL1138E12 5', mRNA sequence.    48   2e-08  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ010466_1(AJ010466|pid:none) Arabidopsis thaliana mRNA for DEAD...   258   1e-67
GM694469_1(GM694469|pid:none) Sequence 25 from Patent EP1953235.      256   4e-67
(O13792) RecName: Full=ATP-dependent RNA helicase uap56;        ...   255   5e-67
D89270_1(D89270|pid:none) Schizosaccharomyces pombe mRNA, partia...   255   7e-67
GM694505_1(GM694505|pid:none) Sequence 61 from Patent EP1953235....   255   7e-67
BT068295_1(BT068295|pid:none) Zea mays full-length cDNA clone ZM...   254   9e-67
(Q56XG6) RecName: Full=DEAD-box ATP-dependent RNA helicase 15;  ...   254   1e-66
(A1DL85) RecName: Full=ATP-dependent RNA helicase sub2;         ...   254   1e-66
AL360314_6(AL360314|pid:none) Arabidopsis thaliana DNA chromosom...   254   1e-66
EU963228_1(EU963228|pid:none) Zea mays clone 259532 spliceosome ...   254   2e-66

 psg: 0.75  gvh: 0.46  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFG486 is cyt

>AFG486Z.Seq
----------TTTACAATTAGCATCTGAAGGTGCACTCTCACTCAAAGAGATTAAACAAT
TCATTCTTGATGAATGTGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTACAAA
AGATCTTCAAACTCATCCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCTCTG
ATACCATTCGTCCAATCTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCAATG
ATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAGAAC
AAAAGAATAAAAAACTCATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTATTT
TCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTTTCC
CATCCATCTGTATTCATCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTAAAT
TCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCGATA
TTGAACGTGTCAATGTCGTCATCAACTACGATATGGCTGAATCTGCC