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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK630
Library CH
(Link to library)
Clone ID CHK630
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10823-1
Original site URL
Representative seq. ID CHK630P
(Link to Original site)
Representative DNA sequence 
>CHK630 (CHK630Q) /CSM/CH/CHK6-B/CHK630Q.Seq.d/
GGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTXXXXXXXXXXAATGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCT
CCATTCGATGTAATCAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACC
TCCTCATATGTTACACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTA
CCATTTGAATTGATTATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTG
GATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGAT
GTTGTCTCCTCACCATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAAT
TATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTT
CAACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCGATTCAACTC
TCTCTGTTTCAAATTTACAACTGCTTTAGAGAATCCTT
sequence update 2002.10.25
Translated Amino Acid sequence 
gvlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYY---

---NGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLP
FELIIALDSSLQATGQLFLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTILNNNY
NGTASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSIQLSLFQIYNCFRES
Translated Amino Acid sequence (All Frames) 
Frame A:
gvlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYY---

---NGSLVQSVGTHQTLNAPFDVINVHMRGGNIIPTQPTSSYVTPVDGIPITTKISRTLP
FELIIALDSSLQATGQLFLDDGESIQTYVDNKYSFIQFDVVSSPSSSAYKLQSTILNNNY
NGTASLIINSIQIYGSPSVQQVIVNGSPINSFNAVSIQLSLFQIYNCFRES

Frame B:
vc*kyklklkkk*nfclh*lfflllyyfxlldhyqhhyvniqviqlkxlqkqimvmrqhl
tlfqqvhmvtixnn*ifn*llklvkflxlxlltqiikdgkshqllin*lxkixiqlii*l
nlqiihlvlqqlvfqlxkfyliqlnqvivhlmv*fiqiii---

---mvhwfnqlvpikl*mlhsm*stficvvvisfqlnqpphmlhqlmvfqlplkslelyh
ln*llpwilhykqlvnyswmmvnqfkpmliintlsfnsmlsphhlhlptnynqpfsitii
mvpll*slilskstvrhqfnksllmvaqsihlmlfrfnslcfkfttalenp

Frame C:
cvknin*n*kknetfvfinyffcyciilxcwiiisiim*isrlfnsrxyknk*wl*gnt*
pyfsrsiw*ryxtikfsinf*n*snfxsxny*pk*skmgsptnc*siswxksxfn*lyn*
iyk*siwfcsnsyfnwxsfi*ynstk*lfi*wfnlfkllf---

---wfigsiswypsnfkcsircnqrsyaww*yhsnstnllicyts*wysnyh*nl*nfti
*idyclgffitsnwsiilg*w*insnlc***ilfhsircclltificlqitinhsq*ql*
wyrffnh*fypnlrfaisstshc*w*pnqfi*ccfdstlsvsnlqll*ril
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK630 (CHK630Q) /CSM/CH/CHK6-B/CHK630Q.Seq.d/                       1764   0.0
VHJ526 (VHJ526Q) /CSM/VH/VHJ5-B/VHJ526Q.Seq.d/                        989   0.0
CHN654 (CHN654Q) /CSM/CH/CHN6-C/CHN654Q.Seq.d/                        989   0.0
AHM675 (AHM675Q) /CSM/AH/AHM6-D/AHM675Q.Seq.d/                        989   0.0
AHJ427 (AHJ427Q) /CSM/AH/AHJ4-B/AHJ427Q.Seq.d/                        979   0.0
SHG203 (SHG203Q) /CSM/SH/SHG2-A/SHG203Q.Seq.d/                        975   0.0
CHS485 (CHS485Q) /CSM/CH/CHS4-D/CHS485Q.Seq.d/                        975   0.0
CHR138 (CHR138Q) /CSM/CH/CHR1-B/CHR138Q.Seq.d/                        975   0.0
CHO239 (CHO239Q) /CSM/CH/CHO2-B/CHO239Q.Seq.d/                        975   0.0
CHL607 (CHL607Q) /CSM/CH/CHL6-A/CHL607Q.Seq.d/                        975   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    40   3e-05  19
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           42   3e-04  24
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   6e-04  15
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    32   8e-04  15
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    32   0.002  17
CP000029|CP000029.1 Staphylococcus epidermidis RP62A, complete genome.    32   0.005  28
BA000026|BA000026.2 Mycoplasma penetrans HF-2 DNA, complete genome.    32   0.005  24
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   0.008  16
AC151956|AC151956.3 Medicago truncatula clone mth2-52p17, WORKING DRAFT SEQUENCE, 2 ordered pieces.    38   0.009  10
AE014841|AE014841.1 Plasmodium falciparum 3D7 chromosome 11 section 6 of 8 of the complete sequence.    34   0.012  13
dna update 2005. 2.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU937530_1(EU937530|pid:none) Mus musculus sucrase-isomaltase mR...    87   1e-15
L25926_1(L25926|pid:none) Rat sucrase-isomaltase (SI) mRNA, comp...    83   1e-14
(P23739) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    83   1e-14
AF118226_1(AF118226|pid:none) Hordeum vulgare high pI alpha-gluc...    77   9e-13
U49351_1(U49351|pid:none) Mus musculus lysosomal alpha-glucosida...    76   2e-12
(P70699) RecName: Full=Lysosomal alpha-glucosidase;          EC=...    75   3e-12
(Q43763) RecName: Full=Alpha-glucosidase;          EC=3.2.1.20; ...    75   3e-12
BC010210_1(BC010210|pid:none) Mus musculus glucosidase, alpha, a...    75   3e-12
BC115034_1(BC115034|pid:none) Homo sapiens sucrase-isomaltase (a...    75   3e-12
BC116452_1(BC116452|pid:none) Homo sapiens sucrase-isomaltase (a...    75   3e-12
protein update 2009. 4. 7
PSORT 

 psg: 1.02  gvh: 0.72  alm: 0.41  top: 0.27  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for CHK630 is exc
5' end seq. ID CHK630F
5' end seq. 
>CHK630F.Seq
GGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTNNNNNNNNNN
Length of 5' end seq. 492
3' end seq. ID CHK630Z
3' end seq. 
>CHK630Z.Seq
NNNNNNNNNNAATGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCTCC
ATTCGATGTAATCAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACCTC
CTCATATGTTACACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTACC
ATTTGAATTGATTATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTGGA
TGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGATGT
TGTCTCCTCACCATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAATTA
TAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTTCA
ACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCGATTCAACTCTC
TCTGTTTCAAATTTACAACTGCTTTAGAGAATCCTT
Length of 3' end seq. 516
Connected seq. ID CHK630P
Connected seq. 
>CHK630P.Seq
GGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TT----------AATGGTTCATTGGTTCAATCAGTTGGTACCCATCAAACTTTAAATGCT
CCATTCGATGTAATCAACGTTCATATGCGTGGTGGTAATATCATTCCAACTCAACCAACC
TCCTCATATGTTACACCAGTTGATGGTATTCCAATTACCACTAAAATCTCTAGAACTTTA
CCATTTGAATTGATTATTGCCTTGGATTCTTCATTACAAGCAACTGGTCAATTATTCTTG
GATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTTCATTCAATTCGAT
GTTGTCTCCTCACCATCTTCATCTGCCTACAAATTACAATCAACCATTCTCAATAACAAT
TATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATCTACGGTTCGCCATCAGTT
CAACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTGTTTCGATTCAACTC
TCTCTGTTTCAAATTTACAACTGCTTTAGAGAATCCTT
Length of connected seq. 988
Full length Seq ID -
Full length Seq. -
Length of full length seq. -