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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK548
Library CH
(Link to library)
Clone ID CHK548
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID CHK548P
(Link to Original site)
Representative DNA sequence 
>CHK548 (CHK548Q) /CSM/CH/CHK5-B/CHK548Q.Seq.d/
AGGTGGTGTAACCCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGC
ATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCTTG
TACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTGTGATGA
TAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAAT
CAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGTCAC
TCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGA
AGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCTTGATTC
CTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAATCCATG
TACCATAGATTCATGCAATAATTCAACTGGTTGTTGCAACACTCCAATCAATGTTGATGA
TAATAATCCATGTACCGTAGATTCATGTACTAAATCAAGTGGTGTCACTCATACTCCAGT
CAATGTCGATGATAATAACAAATGTACCACTGATGCATGTACTAAAGAAAGTGGTGTAAC
TCATACTCCAATCTCTTGTGATGATAATAATGCCTGTACAATTGATTCATGTTCAAACTC
AACTGGTTGTGTAAACACTCCCNTTAGCTGTGATGATANAAACCCATGTACTGGTTGATA
CATGTACAAAAXXXXXXXXXXACNAACCNTATAATGTTNTCCCNAGTGGNTTTAATTTAA
TNTNTGGNTTAANTGGNGGTNTCNTTGGNGGTGGNACAGGAGGTAAANGNGATTGCNAAA
CNTGTAAAAATTAAANAACTCNTTTTATTTAATTATATTTTAAAAAAATTCNAAAAAAAT
TCNAAAAAAAATTCNAGAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATT
TTTAAAAATAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
vv*pipqsilmitmpvqlmhvqkkvv*lilqsilmitmlvpltpvhhqlafptpqltvmi
vihvp*thvqiqpvvatlqsmlmiiihvl*ihvpnqqvslipqsm*mittnvqlmhvpkk
vv*lilqsilmitmhvplipvhhqlvfptlqltvmivihvp*ihaiiqlvvatlqsmlmi
iihvp*ihvlnqvvslilqsmsmiitnvplmhvlkkvv*lilqslvmiimpvqlihvqtq
lvv*TLPLAVMIXTHVLVDTCTK---

---XPYNVXPSGFNLXXGLXGGXXGGGTGGKXDCXTCKN*xtxfi*lyfkkixkkfxkkf
xkkkklfvflsniynf*k*k
Translated Amino Acid sequence (All Frames) 
Frame A:
rwcnpypsqy***qclyn*cmykrrwcnsyssqy***qclyp*llftinwrfphpn*l**
**smyrrlmfkfnrllqhsnqc****smycrfmyqtnrchsypsqcr**qqmyn*cmyqr
rwcnsyssqy***qcmyp*flftinwyfprsn*l****smyhrfmq*fnwllqhsnqc**
**smyrrfmy*ikwchsyssqcr***qmyh*cmy*rkwcnsysnll****clyn*fmfkl
nwlckhsx*l**xkpmywlihvq---

---tnximxsxvxli*xxx*xxvslxvxqevxxiakxvkikxlxlfnyilkkfxknskkn
srkkknylfsylifiifknkn

Frame B:
ggvthtpvntddnnactidactkeggvthtpvntddnnactldscspstgvshtpincdd
snpctvdscsnstgccntpinvddnnpctvdsctkptgvthtpvnvddnnkctidactke
ggvthtpvntddnnactldscspstgishapincddsnpctidscnnstgccntpinvdd
nnpctvdsctkssgvthtpvnvddnnkcttdactkesgvthtpiscddnnactidscsns
tgcvntpxscddxnpctg*ymyk---

---xtx*cxpxwx*fnxwxxwxxxwxwxrr*xxlxnx*klxnsfyliif*knskkixkki
xekkkticfli*yl*flkiki

Frame C:
vv*pipqsilmitmpvqlmhvqkkvv*lilqsilmitmlvpltpvhhqlafptpqltvmi
vihvp*thvqiqpvvatlqsmlmiiihvl*ihvpnqqvslipqsm*mittnvqlmhvpkk
vv*lilqsilmitmhvplipvhhqlvfptlqltvmivihvp*ihaiiqlvvatlqsmlmi
iihvp*ihvlnqvvslilqsmsmiitnvplmhvlkkvv*lilqslvmiimpvqlihvqtq
lvv*TLPLAVMIXTHVLVDTCTK---

---XPYNVXPSGFNLXXGLXGGXXGGGTGGKXDCXTCKN*xtxfi*lyfkkixkkfxkkf
xkkkklfvflsniynf*k*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK548 (CHK548Q) /CSM/CH/CHK5-B/CHK548Q.Seq.d/                       1675   0.0
SHF749 (SHF749Q) /CSM/SH/SHF7-C/SHF749Q.Seq.d/                       1376   0.0
CHM421 (CHM421Q) /CSM/CH/CHM4-A/CHM421Q.Seq.d/                       1330   0.0
CHF469 (CHF469Q) /CSM/CH/CHF4-C/CHF469Q.Seq.d/                       1318   0.0
SHG235 (SHG235Q) /CSM/SH/SHG2-B/SHG235Q.Seq.d/                       1253   0.0
CHQ124 (CHQ124Q) /CSM/CH/CHQ1-A/CHQ124Q.Seq.d/                       1231   0.0
CHR337 (CHR337Q) /CSM/CH/CHR3-B/CHR337Q.Seq.d/                       1116   0.0
SHF211 (SHF211Q) /CSM/SH/SHF2-A/SHF211Q.Seq.d/                       1076   0.0
CHI784 (CHI784Q) /CSM/CH/CHI7-D/CHI784Q.Seq.d/                       1021   0.0
CHE515 (CHE515Q) /CSM/CH/CHE5-A/CHE515Q.Seq.d/                       1009   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               72   1e-23  7
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    62   6e-09  11
AE014845|AE014845.1 Plasmodium falciparum 3D7 chromosome 12, section 2 of 9 of the complete sequence.    34   5e-08  16
AC100096|AC100096.1 Mus musculus clone RP23-38A11, LOW-PASS SEQUENCE SAMPLING.    48   8e-06  3
CR382399|CR382399.1 Plasmodium falciparum chromosome 6, complete sequence; segment 2/5.    36   5e-05  18
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34   1e-04  13
AE014819|AE014819.1 Plasmodium falciparum 3D7 chromosome 14 section 4 of 13 of the complete sequence.    38   1e-04  10
AG913288|AG913288.1 Drosophila auraria DNA, clone: DAB1-008N15.F.fa, genomic survey sequence.    46   3e-04  2
AJ548837|AJ548837.1 Dictyostelium discoideum mRNA for extracellular signalling molecule  DicA (dicA gene).    44   4e-04  3
AJ548838|AJ548838.1 Dictyostelium discoideum dicA gene for extracellular signalling  molecule DicA, exons 1-2.    44   6e-04  3
dna update 2005. 2.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   428   e-118
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   428   e-118
A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   419   e-116
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   357   4e-97
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   320   6e-86
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   305   1e-81
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                147   4e-34
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                145   3e-33
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                137   4e-31
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                120   6e-26
protein update 2009. 4. 7
PSORT 

 psg: 0.93  gvh: 0.60  alm: 0.38  top: 0.50  tms: 0.00  mit: 0.04  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 12.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: peroxisomal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for CHK548 is cyt
5' end seq. ID CHK548F
5' end seq. 
>CHK548F.Seq
AGGTGGTGTAACCCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGC
ATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCTTG
TACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTGTGATGA
TAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAAT
CAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGTCAC
TCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGA
AGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCTTGATTC
CTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAATCCATG
TACCATAGATTCATGCAATAATTCAACTGGTTGTTGCAACACTCCAATCAATGTTGATGA
TAATAATCCATGTACCGTAGATTCATGTACTAAATCAAGTGGTGTCACTCATACTCCAGT
CAATGTCGATGATAATAACAAATGTACCACTGATGCATGTACTAAAGAAAGTGGTGTAAC
TCATACTCCAATCTCTTGTGATGATAATAATGCCTGTACAATTGATTCATGTTCAAACTC
AACTGGTTGTGTAAACACTCCCNTTAGCTGTGATGATANAAACCCATGTACTGGTTGATA
CATGTACAAAANNNNNNNNNN
Length of 5' end seq. 801
3' end seq. ID CHK548Z
3' end seq. 
>CHK548Z.Seq
NNNNNNNNNNACNAACCNTATAATGTTNTCCCNAGTGGNTTTAATTTAATNTNTGGNTTA
ANTGGNGGTNTCNTTGGNGGTGGNACAGGAGGTAAANGNGATTGCNAAACNTGTAAAAAT
TAAANAACTCNTTTTATTTAATTATATTTTAAAAAAATTCNAAAAAAATTCNAAAAAAAA
TTCNAGAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAATAA
AAATT
Length of 3' end seq. 245
Connected seq. ID CHK548P
Connected seq. 
>CHK548P.Seq
AGGTGGTGTAACCCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGC
ATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCTTG
TACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTGTGATGA
TAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAAT
CAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGTCAC
TCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGA
AGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCTTGATTC
CTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAATCCATG
TACCATAGATTCATGCAATAATTCAACTGGTTGTTGCAACACTCCAATCAATGTTGATGA
TAATAATCCATGTACCGTAGATTCATGTACTAAATCAAGTGGTGTCACTCATACTCCAGT
CAATGTCGATGATAATAACAAATGTACCACTGATGCATGTACTAAAGAAAGTGGTGTAAC
TCATACTCCAATCTCTTGTGATGATAATAATGCCTGTACAATTGATTCATGTTCAAACTC
AACTGGTTGTGTAAACACTCCCNTTAGCTGTGATGATANAAACCCATGTACTGGTTGATA
CATGTACAAAA----------ACNAACCNTATAATGTTNTCCCNAGTGGNTTTAATTTAA
TNTNTGGNTTAANTGGNGGTNTCNTTGGNGGTGGNACAGGAGGTAAANGNGATTGCNAAA
CNTGTAAAAATTAAANAACTCNTTTTATTTAATTATATTTTAAAAAAATTCNAAAAAAAT
TCNAAAAAAAATTCNAGAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATT
TTTAAAAATAAAAATT
Length of connected seq. 1026
Full length Seq ID -
Full length Seq. -
Length of full length seq. -