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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK456
Library CH
(Link to library)
Clone ID CHK456
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15363-1
Original site URL
Representative seq. ID CHK456P
(Link to Original site)
Representative DNA sequence 
>CHK456 (CHK456Q) /CSM/CH/CHK4-C/CHK456Q.Seq.d/
ATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGATGGCCCGTACAAAACAAACCG
CACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACATTGGTTCTAAACAAGCCCATA
AACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGAAAGTACATCGTTTCAGACCAG
GTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGAGTACCGATCTCTTAATTCGTA
AACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAGAATTCAAAACCGATCTTCGTT
TCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTGAAGCTTACTTGGTTGGTTTAT
TCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTGTTACTATCATGCCAAAAGATA
TTCACTTAGCCAGACGTATCAGAGGTGAAAGATCATAAAGAGTTTATTTTTATTTTTTAA
CAAAAAAAAAAXXXXXXXXXXATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGA
TGGCCCGTACAAAACAAACCGCACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACA
TTGGTTCTAAACAAGCCCATAAACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGA
AAGTACATCGTTTCAGACCAGGTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGA
GTACCGATCTCTTAATTCGTAAACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAG
AATTCAAAACCGATCTTCGTTTCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTG
AAGCTTACTTGGTTGGTTTATTCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTG
TTACTATCATGCCAAAAGATATTCACTTAGCCAGACGTAT
sequence update 2002.10.25
Translated Amino Acid sequence 
fllhislkyilkdgpyktnrt*inwc*stt*thwf*tsp*tnsslfikwwckestsfqtr
ycrs*rnqkipkeyrslns*tpiptfsqrnrtriqnrssfpicrhrcpsrsl*sllgwfi
rryqfmchpr*tcyyhakryslsqtyqr*KIIKSLFLFFNKKK---

---FLLHISLKYILKDGPYKTNRT*inwc*stt*thwf*tsp*tnsslfikwwckestsf
qtrycrs*rnqkipkeyrslns*tpiptfsqrnrtriqnrssfpicrhrcpsrsl*sllg
wfirryqfmchpr*tcyyhakryslsqty
Translated Amino Acid sequence (All Frames) 
Frame A:
ifithia*vyfkrwpvqnkphvnqlvlkyhvntlvlnkpinklqslhqvvv*rkyivsdq
vlslleksentkrvpis*fvnshsnv*sekshknskpifvsnlppsvpfkkplkltwlvy
skipiyvpstlnvllscqkift*pdvsevkdhkefifif*qkk---

---ifithia*vyfkrwpvqnkphvnqlvlkyhvntlvlnkpinklqslhqvvv*rkyiv
sdqvlslleksentkrvpis*fvnshsnv*sekshknskpifvsnlppsvpfkkplkltw
lvyskipiyvpstlnvllscqkift*pdv

Frame B:
fllhislkyilkdgpyktnrt*inwc*stt*thwf*tsp*tnsslfikwwckestsfqtr
ycrs*rnqkipkeyrslns*tpiptfsqrnrtriqnrssfpicrhrcpsrsl*sllgwfi
rryqfmchpr*tcyyhakryslsqtyqr*KIIKSLFLFFNKKK---

---FLLHISLKYILKDGPYKTNRT*inwc*stt*thwf*tsp*tnsslfikwwckestsf
qtrycrs*rnqkipkeyrslns*tpiptfsqrnrtriqnrssfpicrhrcpsrsl*sllg
wfirryqfmchpr*tcyyhakryslsqty

Frame C:
fyytyrlsif*kmartkqtarkstgakvprkhigskqahkqtpvssssggvkkvhrfrpg
tvalreirkyqkstdllirklpfqrlvreiaqefktdlrfqsaaigalqeaseaylvglf
edtnlcaihakrvtimpkdihlarrirgers*rvyfyfltkkk---

---fyytyrlsif*kmartkqtarkstgakvprkhigskqahkqtpvssssggvkkvhrf
rpgtvalreirkyqkstdllirklpfqrlvreiaqefktdlrfqsaaigalqeaseaylv
glfedtnlcaihakrvtimpkdihlarr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHN431 (CHN431Q) /CSM/CH/CHN4-B/CHN431Q.Seq.d/                        916   0.0
CHK456 (CHK456Q) /CSM/CH/CHK4-C/CHK456Q.Seq.d/                        916   0.0
SSE866 (SSE866Q) /CSM/SS/SSE8-C/SSE866Q.Seq.d/                        896   0.0
AHJ541 (AHJ541Q) /CSM/AH/AHJ5-B/AHJ541Q.Seq.d/                        892   0.0
CHS113 (CHS113Q) /CSM/CH/CHS1-A/CHS113Q.Seq.d/                        888   0.0
SSM623 (SSM623Q) /CSM/SS/SSM6-A/SSM623Q.Seq.d/                        886   0.0
VSC276 (VSC276Q) /CSM/VS/VSC2-D/VSC276Q.Seq.d/                        882   0.0
SSM830 (SSM830Q) /CSM/SS/SSM8-B/SSM830Q.Seq.d/                        882   0.0
SSK765 (SSK765Q) /CSM/SS/SSK7-C/SSK765Q.Seq.d/                        882   0.0
SSL709 (SSL709Q) /CSM/SS/SSL7-A/SSL709Q.Seq.d/                        880   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U76664|U76664.1 Dictyostelium discoideum histone H3 (DdH3) mRNA, complete cds.   833   0.0    1
CK811270|CK811270.1 Rasgsc6766 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.   135   2e-35  2
CK809234|CK809234.1 Rasgsc2618 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.   135   2e-35  2
CK808367|CK808367.1 Rasgsc0933 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.   131   4e-34  2
CO643994|CO643994.1 USDA-FP_112733 Adult Glassy-winged Sharpshooter Homalodisca coagulata cDNA clone WHHC095_E08 5', mRNA sequence.    74   4e-33  3
AF378198|AF378198.1 Rhynchosciara americana histone H1 (rah1), histone H3 (rah3), histone H4 (rah4), histone H2A (rah2a), and histone H2B (rah2b) genes, complete cds.   113   2e-32  3
BU099641|BU099641.1 WHE3309_G08_M15ZS Chinese Spring wheat drought stressed root cDNA library Triticum aestivum cDNA clone WHE3309_G08_M15, mRNA sequence.   149   1e-31  1
CK811838|CK811838.1 Rasgsc7876 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.   113   1e-31  3
CK809333|CK809333.1 Rasgsc2796 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    80   5e-31  3
CK810967|CK810967.1 Rasgsc6283 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.   117   5e-31  3
dna update 2005. 2. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P84243) RecName: Full=Histone H3.3;  &(P84245) RecName: Fu &(P8...   215   2e-54
(Q10453) RecName: Full=Histone H3.3 type 1;  &FB335644_1(FB33564...   215   2e-54
AY813016_1(AY813016|pid:none) Schistosoma japonicum SJCHGC01198 ...   214   2e-54
(Q8WSF1) RecName: Full=Histone H3.3;  &AF323993_1(AF323993|pid:n...   214   4e-54
AY533212_1(AY533212|pid:none) Chinchilla lanigera histone H3.3B ...   214   4e-54
(Q9U281) RecName: Full=Histone H3.3 type 2;  &FB335642_1(FB33564...   214   4e-54
AJ277902_1(AJ277902|pid:none) Paracentrotus lividus partial h3.3...   213   5e-54
CS560304_1(CS560304|pid:none) Sequence 891 from Patent WO2006032...   213   5e-54
AK291102_1(AK291102|pid:none) Homo sapiens cDNA FLJ78487 complet...   213   5e-54
BC021768_1(BC021768|pid:none) Mus musculus H3 histone, family 3B...   213   7e-54
protein update 2009. 4. 7
PSORT 

 psg: 0.79  gvh: 0.22  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for CHK456 is nuc
5' end seq. ID CHK456F
5' end seq. 
>CHK456F.Seq
ATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGATGGCCCGTACAAAACAAACCG
CACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACATTGGTTCTAAACAAGCCCATA
AACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGAAAGTACATCGTTTCAGACCAG
GTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGAGTACCGATCTCTTAATTCGTA
AACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAGAATTCAAAACCGATCTTCGTT
TCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTGAAGCTTACTTGGTTGGTTTAT
TCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTGTTACTATCATGCCAAAAGATA
TTCACTTAGCCAGACGTATCAGAGGTGAAAGATCATAAAGAGTTTATTTTTATTTTTTAA
CAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 501
3' end seq. ID CHK456Z
3' end seq. 
>CHK456Z.Seq
NNNNNNNNNNATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGATGGCCCGTACA
AAACAAACCGCACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACATTGGTTCTAAA
CAAGCCCATAAACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGAAAGTACATCGT
TTCAGACCAGGTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGAGTACCGATCTC
TTAATTCGTAAACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAGAATTCAAAACC
GATCTTCGTTTCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTGAAGCTTACTTG
GTTGGTTTATTCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTGTTACTATCATG
CCAAAAGATATTCACTTAGCCAGACGTAT
Length of 3' end seq. 449
Connected seq. ID CHK456P
Connected seq. 
>CHK456P.Seq
ATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGATGGCCCGTACAAAACAAACCG
CACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACATTGGTTCTAAACAAGCCCATA
AACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGAAAGTACATCGTTTCAGACCAG
GTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGAGTACCGATCTCTTAATTCGTA
AACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAGAATTCAAAACCGATCTTCGTT
TCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTGAAGCTTACTTGGTTGGTTTAT
TCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTGTTACTATCATGCCAAAAGATA
TTCACTTAGCCAGACGTATCAGAGGTGAAAGATCATAAAGAGTTTATTTTTATTTTTTAA
CAAAAAAAAAA----------ATTTTTATTACACATATCGCTTAAGTATATTTTAAAAGA
TGGCCCGTACAAAACAAACCGCACGTAAATCAACTGGTGCTAAAGTACCACGTAAACACA
TTGGTTCTAAACAAGCCCATAAACAAACTCCAGTCTCTTCATCAAGTGGTGGTGTAAAGA
AAGTACATCGTTTCAGACCAGGTACTGTCGCTCTTAGAGAAATCAGAAAATACCAAAAGA
GTACCGATCTCTTAATTCGTAAACTCCCATTCCAACGTTTAGTCAGAGAAATCGCACAAG
AATTCAAAACCGATCTTCGTTTCCAATCTGCCGCCATCGGTGCCCTTCAAGAAGCCTCTG
AAGCTTACTTGGTTGGTTTATTCGAAGATACCAATTTATGTGCCATCCACGCTAAACGTG
TTACTATCATGCCAAAAGATATTCACTTAGCCAGACGTAT
Length of connected seq. 930
Full length Seq ID -
Full length Seq. -
Length of full length seq. -