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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK330
Library CH
(Link to library)
Clone ID CHK330
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID CHK330P
(Link to Original site)
Representative DNA sequence 
>CHK330 (CHK330Q) /CSM/CH/CHK3-B/CHK330Q.Seq.d/
CTCATGTACTGTTGATACCTGTGATGACTCAACCGGTTGTTGCAATACTCCAATCAATGT
TGATGATAATAATCCATGTACCGTAGATGCCTGTACCAAATCAACAGGTGTCACTCATAC
CCCAGTAAATGTAGATGATAATAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGG
TGTAACTCATACTCCAGTCAATACTGATGATAACAATGCCTGTACTCTTGATNNCTGTTC
ACCATNAACNGGTGTNACTCATACCCCAATAAATTGTGATGATAANAANGCCTGTACTGT
CGACTCATGTTCAAACTCAACTGGTTGCGTAAATACTCCAATNANNTGTGATGATAATAA
TCCATGTACTGTTGATTCTTGTGATGACATAACTGGTGTGANGCAATACTCCAATCAATG
TTGATGATAATAACNCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTCACCTCA
TACCCCAGTTCAATGTGNATGATAATAACAAATGTACAATTGATGCATGTNCCAAAAAAN
GTGGTGTAACTCATACTCCAATCAGTACTGATGATAACAATGCCTGGTACNCTTGATTCC
TGGTTCAAACATNAACTGGTGGTTTNNCACNCCCCCAATTAANNCGTGATGATAATAAAT
ACCTGXXXXXXXXXXAAAACCAAAGAATAAGTGTCAAGTTGCAAAATGTGATTTAATTAA
AGGTTGTACCGTCTCAAATGTAGTATGTGATGATGGTAATGCTTGTACCGAAGATTCATG
TTGTTCAGACACTGGTAAATGTCAATTCGAACCAATCAAACTTCCAAAGAATAAAAACAA
ATGTATCATTTCAAAATGTGATCCAATTAAAGGTACAATCACCAACAGTACCGTAAACTG
TGAATGTGATGACCTTTGTAACATTGGTGAATGTTGTGAAGATACAGGAAAATGTAATTA
CAGACAAAAAGATTGTGATGATAATAATCCAAAAACAGCTGATAGTTGCGATTCCAAGAC
TGGTAAATGTATTAACAAACCATATAATGTTATCACAAGTGGTTCTAATTTAATCTCTGG
TTTAATTGGTGGTCTCATTGGTGGTGGTACAGGAGGTAAAGGTGATTGCAAAACTTGTAA
AAATTAAATAACTCTTTTTATTTAATTATATTTTAAAAAAATTCAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
hvllipvmtqpvvailqsmlmiiihvp*mpvpnqqvslipq*m*miitnvqlmhvpkkvv
*lilqsilmitmpvllxxvhhxxvxlipq*ivmixxpvlsthvqtqlva*ilqxxvmiii
hvllilvmt*lv*xntpinvddnnxmyrrclyqinrchlipqfnvxdnnkctidacxkkx
gvthtpistddnnawyx*FLVQTXTGGXXXPQLXRDDNKYL---

---KPKNKCQVAKCDLIKGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCI
ISKCDPIKGTITNSTVNCECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGK
CINKPYNVITSGSNLISGLIGGLIGGGTGGKGDCKTCKN*itlfi*lyfkkiqkk
Translated Amino Acid sequence (All Frames) 
Frame A:
lmyc*yl**lnrllqysnqc****smyrrclyqinrchsypskcr***qmyn*cmyqrrw
cnsyssqy***qclys*xlftxnxcxsypnkl***xxlycrlmfklnwlrkysnxx****
smyc*fl**hnwcxailqsmlmiitpctvdactkstgvtsypssmxmiitnvqlmhvpkx
vv*lilqsvlmitmpgtldswfkhxlvvxhxpn*xvmiint---

---ktke*vssckm*fn*rlyrlkcsm**w*clyrrfmlfrhw*msirtnqtske*kqmy
hfkm*sn*rynhqqyrkl*m**pl*hw*ml*ryrkm*lqtkrl****skns**lrfqdw*
my*qti*cyhkwf*fnlwfnwwshwwwyrr*r*lqnl*klnnsfyliif*knskk

Frame B:
sctvdtcddstgccntpinvddnnpctvdactkstgvthtpvnvddnnkctidactkegg
vthtpvntddnnactldxcspxtgvthtpincddxxactvdscsnstgcvntpxxcddnn
pctvdscdditgvxqysnqc****xhvpsmpvpnqqvsphtpvqcx***qmyn*cmxqkx
wcnsysnqy***qclvxlipgsnxnwwfxtppixx****ip---

---KPKNKCQVAKCDLIKGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCI
ISKCDPIKGTITNSTVNCECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGK
CINKPYNVITSGSNLISGLIGGLIGGGTGGKGDCKTCKN*itlfi*lyfkkiqkk

Frame C:
hvllipvmtqpvvailqsmlmiiihvp*mpvpnqqvslipq*m*miitnvqlmhvpkkvv
*lilqsilmitmpvllxxvhhxxvxlipq*ivmixxpvlsthvqtqlva*ilqxxvmiii
hvllilvmt*lv*xntpinvddnnxmyrrclyqinrchlipqfnvxdnnkctidacxkkx
gvthtpistddnnawyx*FLVQTXTGGXXXPQLXRDDNKYL---

---nqrisvklqnvi*lkvvpsqm*yvmmvmlvpkihvvqtlvnvnsnqsnfqriktnvs
fqnviqlkvqsptvp*tvnvmtfvtlvnvvkiqenvitdkkivmiiiqkqlivaiprlvn
vltnhimlsqvvli*slv*lvvslvvvqevkviaklvkik*lflfnyilkkfkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK330 (CHK330Q) /CSM/CH/CHK3-B/CHK330Q.Seq.d/                       2137   0.0
SLB280 (SLB280Q) /CSM/SL/SLB2-D/SLB280Q.Seq.d/                        989   0.0
SHG408 (SHG408Q) /CSM/SH/SHG4-A/SHG408Q.Seq.d/                        989   0.0
SHC394 (SHC394Q) /CSM/SH/SHC3-D/SHC394Q.Seq.d/                        989   0.0
SFC263 (SFC263Q) /CSM/SF/SFC2-C/SFC263Q.Seq.d/                        989   0.0
CFF356 (CFF356Q) /CSM/CF/CFF3-C/CFF356Q.Seq.d/                        989   0.0
CHR684 (CHR684Q) /CSM/CH/CHR6-D/CHR684Q.Seq.d/                        975   0.0
SLE718 (SLE718Q) /CSM/SL/SLE7-A/SLE718Q.Seq.d/                        973   0.0
SLC872 (SLC872Q) /CSM/SL/SLC8-C/SLC872Q.Seq.d/                        973   0.0
SHF321 (SHF321Q) /CSM/SH/SHF3-A/SHF321Q.Seq.d/                        969   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               66   9e-25  5
AF134662|AF134662.1 Plasmodium falciparum strain UPV4 from Burkina Faso CG2 omega repeat (cg2) gene, partial cds.    32   2e-06  5
AF134671|AF134671.1 Plasmodium falciparum strain TOG1 from Togo CG2 omega repeat (cg2) gene, partial cds.    32   3e-06  5
AF134676|AF134676.1 Plasmodium falciparum strain CAE6 from Cameroon CG2 omega repeat (cg2) gene, partial cds.    32   4e-06  5
AF134679|AF134679.1 Plasmodium falciparum strain COM4 from Comoros CG2 omega repeat (cg2) gene, partial cds.    32   4e-06  5
AF134677|AF134677.1 Plasmodium falciparum strain IVC20 from Cote d' Ivoire CG2 omega repeat (cg2) gene, partial cds.    32   4e-06  5
AF134686|AF134686.1 Plasmodium falciparum strain IVC10 from Cote d' Ivoire CG2 omega repeat (cg2) gene, partial cds.    32   5e-06  5
AF134684|AF134684.1 Plasmodium falciparum strain SEN22 from Senegal CG2 omega repeat (cg2) gene, partial cds.    32   5e-06  5
AF134687|AF134687.1 Plasmodium falciparum strain SEN13 from Senegal CG2 omega repeat (cg2) gene, partial cds.    32   5e-06  5
AF134693|AF134693.1 Plasmodium falciparum strain EQG3 from Equatorial Guinea CG2 omega repeat (cg2) gene, partial cds.    32   6e-06  5
dna update 2005. 2. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   347   6e-94
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   276   8e-73
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   276   1e-72
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   226   9e-58
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   170   9e-52
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   167   1e-43
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                107   8e-22
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                105   2e-21
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 97   8e-19
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                 96   2e-18
protein update 2009. 4. 3
PSORT 

 psg: 0.84  gvh: 0.39  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar

>> prediction for CHK330 is cyt
5' end seq. ID CHK330F
5' end seq. 
>CHK330F.Seq
CTCATGTACTGTTGATACCTGTGATGACTCAACCGGTTGTTGCAATACTCCAATCAATGT
TGATGATAATAATCCATGTACCGTAGATGCCTGTACCAAATCAACAGGTGTCACTCATAC
CCCAGTAAATGTAGATGATAATAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGG
TGTAACTCATACTCCAGTCAATACTGATGATAACAATGCCTGTACTCTTGATNNCTGTTC
ACCATNAACNGGTGTNACTCATACCCCAATAAATTGTGATGATAANAANGCCTGTACTGT
CGACTCATGTTCAAACTCAACTGGTTGCGTAAATACTCCAATNANNTGTGATGATAATAA
TCCATGTACTGTTGATTCTTGTGATGACATAACTGGTGTGANGCAATACTCCAATCAATG
TTGATGATAATAACNCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTCACCTCA
TACCCCAGTTCAATGTGNATGATAATAACAAATGTACAATTGATGCATGTNCCAAAAAAN
GTGGTGTAACTCATACTCCAATCAGTACTGATGATAACAATGCCTGGTACNCTTGATTCC
TGGTTCAAACATNAACTGGTGGTTTNNCACNCCCCCAATTAANNCGTGATGATAATAAAT
ACCTGNNNNNNNNNN
Length of 5' end seq. 675
3' end seq. ID CHK330Z
3' end seq. 
>CHK330Z.Seq
NNNNNNNNNNAAAACCAAAGAATAAGTGTCAAGTTGCAAAATGTGATTTAATTAAAGGTT
GTACCGTCTCAAATGTAGTATGTGATGATGGTAATGCTTGTACCGAAGATTCATGTTGTT
CAGACACTGGTAAATGTCAATTCGAACCAATCAAACTTCCAAAGAATAAAAACAAATGTA
TCATTTCAAAATGTGATCCAATTAAAGGTACAATCACCAACAGTACCGTAAACTGTGAAT
GTGATGACCTTTGTAACATTGGTGAATGTTGTGAAGATACAGGAAAATGTAATTACAGAC
AAAAAGATTGTGATGATAATAATCCAAAAACAGCTGATAGTTGCGATTCCAAGACTGGTA
AATGTATTAACAAACCATATAATGTTATCACAAGTGGTTCTAATTTAATCTCTGGTTTAA
TTGGTGGTCTCATTGGTGGTGGTACAGGAGGTAAAGGTGATTGCAAAACTTGTAAAAATT
AAATAACTCTTTTTATTTAATTATATTTTAAAAAAATTCAAAAAAAA
Length of 3' end seq. 527
Connected seq. ID CHK330P
Connected seq. 
>CHK330P.Seq
CTCATGTACTGTTGATACCTGTGATGACTCAACCGGTTGTTGCAATACTCCAATCAATGT
TGATGATAATAATCCATGTACCGTAGATGCCTGTACCAAATCAACAGGTGTCACTCATAC
CCCAGTAAATGTAGATGATAATAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGG
TGTAACTCATACTCCAGTCAATACTGATGATAACAATGCCTGTACTCTTGATNNCTGTTC
ACCATNAACNGGTGTNACTCATACCCCAATAAATTGTGATGATAANAANGCCTGTACTGT
CGACTCATGTTCAAACTCAACTGGTTGCGTAAATACTCCAATNANNTGTGATGATAATAA
TCCATGTACTGTTGATTCTTGTGATGACATAACTGGTGTGANGCAATACTCCAATCAATG
TTGATGATAATAACNCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTCACCTCA
TACCCCAGTTCAATGTGNATGATAATAACAAATGTACAATTGATGCATGTNCCAAAAAAN
GTGGTGTAACTCATACTCCAATCAGTACTGATGATAACAATGCCTGGTACNCTTGATTCC
TGGTTCAAACATNAACTGGTGGTTTNNCACNCCCCCAATTAANNCGTGATGATAATAAAT
ACCTG----------AAAACCAAAGAATAAGTGTCAAGTTGCAAAATGTGATTTAATTAA
AGGTTGTACCGTCTCAAATGTAGTATGTGATGATGGTAATGCTTGTACCGAAGATTCATG
TTGTTCAGACACTGGTAAATGTCAATTCGAACCAATCAAACTTCCAAAGAATAAAAACAA
ATGTATCATTTCAAAATGTGATCCAATTAAAGGTACAATCACCAACAGTACCGTAAACTG
TGAATGTGATGACCTTTGTAACATTGGTGAATGTTGTGAAGATACAGGAAAATGTAATTA
CAGACAAAAAGATTGTGATGATAATAATCCAAAAACAGCTGATAGTTGCGATTCCAAGAC
TGGTAAATGTATTAACAAACCATATAATGTTATCACAAGTGGTTCTAATTTAATCTCTGG
TTTAATTGGTGGTCTCATTGGTGGTGGTACAGGAGGTAAAGGTGATTGCAAAACTTGTAA
AAATTAAATAACTCTTTTTATTTAATTATATTTTAAAAAAATTCAAAAAAAA
Length of connected seq. 1182
Full length Seq ID -
Full length Seq. -
Length of full length seq. -