NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK263
Library CH
(Link to library)
Clone ID CHK263
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16475-1|Contig-U16524-1
Original site URL
Representative seq. ID CHK263P
(Link to Original site)
Representative DNA sequence 
>CHK263 (CHK263Q) /CSM/CH/CHK2-C/CHK263Q.Seq.d/
AAAAACAACCATTTATTAATTTTTGTATGATTATTATAATTCAAATTAAAAATATATTAT
ATAAATATATATAAATATATATTAAATAAAAATAATAATAAAATAAATTTAATATAATAA
AAAATTTTTTTGAGAAAAAAAAAAANTAAAAAAAAAAAAAAAAAAAAAAAAACCCNAAAA
AACCCNAAAAAAAAAANCCCNNNAAAAAAAXXXXXXXXXXTTGTAAAATTGGTTATTGTG
AACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTATTG
AAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTGATA
ATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATGGAA
AATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAGTTT
CAACTGCTGTTATTGCAAGTGTTGTTGCAGTTGGTGCTGTTGTT
sequence update 2002.10.25
Translated Amino Acid sequence 
knnhllifv*ll*fklkiyyiniykyilnknnnkinli**KIFLRKKKXKKKKKKKKTXK
NPKKKXPXKK---

---CKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPDNSKCQEGVCVN
NTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVAVGAVV
Translated Amino Acid sequence (All Frames) 
Frame A:
knnhllifv*ll*fklkiyyiniykyilnknnnkinli**KIFLRKKKXKKKKKKKKTXK
NPKKKXPXKK---

---l*nwll*twfrl*ikg*slffrlvly*ssm*ckiwlhsir*kmcs****msrrclck
qycyr*k*wkmyf*rl*skticlskscfsfnccyckcccswccc

Frame B:
kttiy*flydyynsn*kyii*iyiniy*ikiiik*i*ynkkff*ekkkxkkkkkkkkpxk
txkkkxxxk---

---CKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPDNSKCQEGVCVN
NTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVAVGAVV

Frame C:
kqpfinfcmiiiiqiknilykyi*iyik*k***nkfniiknffekkkx*kkkkkkknpkk
pxkkxpxkk---

---vklvivnlvsvvn*riefvlpigivlklnvmqnmvafnsiedvlliivnvkkvsv*t
illsvkmmenvflkimiqnhlfvkkll*fqllllqvllqlvll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK263 (CHK263Q) /CSM/CH/CHK2-C/CHK263Q.Seq.d/                        603   e-171
CHQ874 (CHQ874Q) /CSM/CH/CHQ8-D/CHQ874Q.Seq.d/                        595   e-169
SSG816 (SSG816Q) /CSM/SS/SSG8-A/SSG816Q.Seq.d/                        595   e-169
SSD840 (SSD840Q) /CSM/SS/SSD8-B/SSD840Q.Seq.d/                        595   e-169
SSD152 (SSD152Q) /CSM/SS/SSD1-C/SSD152Q.Seq.d/                        595   e-169
SLF208 (SLF208Q) /CSM/SL/SLF2-A/SLF208Q.Seq.d/                        595   e-169
SLE246 (SLE246Q) /CSM/SL/SLE2-B/SLE246Q.Seq.d/                        595   e-169
SLD406 (SLD406Q) /CSM/SL/SLD4-A/SLD406Q.Seq.d/                        595   e-169
SLB442 (SLB442Q) /CSM/SL/SLB4-B/SLB442Q.Seq.d/                        595   e-169
SLB259 (SLB259Q) /CSM/SL/SLB2-C/SLB259Q.Seq.d/                        595   e-169
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    66   7e-07  1
CC946427|CC946427.1 BOIFV20TF BO_1.4_1.6_KB_nuc Brassica oleracea genomic clone BOIFV20, genomic survey sequence.    50   0.039  1
BH960705|BH960705.1 odd88f07.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    50   0.039  1
BF050079|BF050079.1 As_tgz_22C04_SKPL Ascaris suum adult male testis germinal zone from Alan Scott Ascaris suum cDNA clone As_tgz_22C04 5', mRNA sequence.    50   0.039  1
BM278751|BM278751.1 As_tgz_66H01_SKPL Ascaris suum adult male testis germinal zone from Alan Scott Ascaris suum cDNA clone As_tgz_66H01 5', mRNA sequence.    50   0.039  1
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    38   0.30   5
AC115502|AC115502.4 Rattus norvegicus clone CH230-291D21, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    42   1.0    3
AC019256|AC019256.4 Homo sapiens chromosome 4 clone RP11-426C17 map 4, WORKING DRAFT SEQUENCE, 24 unordered pieces.    44   1.4    5
AC150749|AC150749.1 Pan troglodytes chromosome 7 clone CH251-553P23, WORKING DRAFT SEQUENCE, 13 unordered pieces.    34   1.8    6
CR752643|CR752643.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-186G11.    44   2.4    1
dna update 2005. 2. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                191   1e-47
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   106   4e-22
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...    96   5e-19
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...    92   7e-18
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                 89   8e-17
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                 76   6e-13
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 64   3e-09
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                 40   0.026
AF501275_1(AF501275|pid:none) Entamoeba histolytica clone C3 Gal...    37   0.28
(Q1ZXC5) RecName: Full=Protein psiC; Flags: Precursor;                 37   0.28
protein update 2009. 4. 3
PSORT 

 psg: 0.22  gvh: 0.42  alm: 0.21  top: 0.60  tms: 0.07  mit: 0.35  mip: 0.02
 nuc: 0.69  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for CHK263 is cyt
5' end seq. ID CHK263F
5' end seq. 
>CHK263F.Seq
AAAAACAACCATTTATTAATTTTTGTATGATTATTATAATTCAAATTAAAAATATATTAT
ATAAATATATATAAATATATATTAAATAAAAATAATAATAAAATAAATTTAATATAATAA
AAAATTTTTTTGAGAAAAAAAAAAANTAAAAAAAAAAAAAAAAAAAAAAAAACCCNAAAA
AACCCNAAAAAAAAAANCCCNNNAAAAAAANNNNNNNNNN
Length of 5' end seq. 220
3' end seq. ID CHK263Z
3' end seq. 
>CHK263Z.Seq
NNNNNNNNNNTTGTAAAATTGGTTATTGTGAACCTGGTTTCGGTTGTAAATTAAAGGATA
GAGTTTGTTCTTCCGATTGGTATTGTATTGAAGCTCAATGTGATGCAAAATATGGTTGCA
TTCAATTCGATAGAAGATGTGCTCCTGATAATAGTAAATGTCAAGAAGGTGTCTGTGTAA
ACAATACTGCTATCGGTAAAAATGATGGAAAATGTATTTCTGAAGATTATGATCCAAAAC
CATTTGTTTGTCAAAAAGCTGCTTTAGTTTCAACTGCTGTTATTGCAAGTGTTGTTGCAG
TTGGTGCTGTTGTT
Length of 3' end seq. 314
Connected seq. ID CHK263P
Connected seq. 
>CHK263P.Seq
AAAAACAACCATTTATTAATTTTTGTATGATTATTATAATTCAAATTAAAAATATATTAT
ATAAATATATATAAATATATATTAAATAAAAATAATAATAAAATAAATTTAATATAATAA
AAAATTTTTTTGAGAAAAAAAAAAANTAAAAAAAAAAAAAAAAAAAAAAAAACCCNAAAA
AACCCNAAAAAAAAAANCCCNNNAAAAAAA----------TTGTAAAATTGGTTATTGTG
AACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTATTG
AAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTGATA
ATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATGGAA
AATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAGTTT
CAACTGCTGTTATTGCAAGTGTTGTTGCAGTTGGTGCTGTTGTT
Length of connected seq. 514
Full length Seq ID -
Full length Seq. -
Length of full length seq. -