>CHK244 (CHK244Q) /CSM/CH/CHK2-B/CHK244Q.Seq.d/
XXXXXXXXXXCAATTCATGTCGATGCAGCAAGTGGTGGTTTCGTTGCACCCTTTCTTCAA
CCAGAAATTGTTTGGGATTTTAGACTACCACGTGTTAAATCAATTAATGGCTCTGGTCAT
AAATTTGGTCTATCTCCATTGGGAGTTGGCTGGGTCGTATGGAGAGGTAAAAATGACATT
CACAAGGATTTGGTATTCGATGTCAACTATCTTGGTGGCAATATGCCAACATTTTCATTG
AATTTCTCACGTCCAGGTGGTCAAATAGTATGCCAGTACTATAACTTTTTGCGCCATGGT
CGTAGTGGATATACCGCAGTTCATCAAGCTTGTCTAGATCATGGTATTTTCATTTCCAAG
CAAGTTAAAAAACACGGTATTTTTGATATTGTTTATGATGGTACTGGTGCACTTCCAGGT
GCATGTTGGAAATTGAAAAAAGATGCAAAAGTAAAATTCAATCTCTTTGACCTTTCAGAT
AGAATGAGAAATAGAGGTTGGCAAATTGCATCCTATACTCTACCAAACTTACCAGATATT
GTAGTTCAAAGAGTATTAATTAGACATGGTTTCAGTCATG

---IHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKFGLSPLGVGWVVWRGKNDIH
KDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRSGYTAVHQACLDHGIFISKQ
VKKHGIFDIVYDGTGALPGACWKLKKDAKVKFNLFDLSDRMRNRGWQIASYTLPNLPDIV
VQRVLIRHGFSH

Frame A:
---nscrcskwwfrctlsstrnclgf*tttc*in*wlws*iwsisigswlgrmer*k*hs
qgfgircqlswwqyanifiefltsrwsnsmpvl*lfapws*wiyrsssslsrswyfhfqa
s*ktryf*ycl*wywctsrcmleiekrckskiqsl*pfr*nek*rlancilystkltryc
ssksin*twfqs


Frame B:
---IHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKFGLSPLGVGWVVWRGKNDIH
KDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRSGYTAVHQACLDHGIFISKQ
VKKHGIFDIVYDGTGALPGACWKLKKDAKVKFNLFDLSDRMRNRGWQIASYTLPNLPDIV
VQRVLIRHGFSH


Frame C:
---fmsmqqvvvslhpffnqklfgildyhvlnqlmalvinlvylhwelagsygevkmtft
riwysmstilvaicqhfh*ishvqvvk*yastitfcamvvvdipqfiklv*imvfsfpsk
lkntvflilfmmvlvhfqvhvgn*kkmqk*nsisltfqie*eievgklhpilyqtyqil*
fkey*ldmvsvm

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB722 (SLB722Q) /CSM/SL/SLB7-A/SLB722Q.Seq.d/                       1130   0.0
SFL288 (SFL288Q) /CSM/SF/SFL2-D/SFL288Q.Seq.d/                       1130   0.0
SFK622 (SFK622Q) /CSM/SF/SFK6-A/SFK622Q.Seq.d/                       1130   0.0
SFJ796 (SFJ796Q) /CSM/SF/SFJ7-D/SFJ796Q.Seq.d/                       1130   0.0
SFJ789 (SFJ789Q) /CSM/SF/SFJ7-D/SFJ789Q.Seq.d/                       1130   0.0
SFJ443 (SFJ443Q) /CSM/SF/SFJ4-B/SFJ443Q.Seq.d/                       1130   0.0
SFH461 (SFH461Q) /CSM/SF/SFH4-C/SFH461Q.Seq.d/                       1130   0.0
SFG840 (SFG840Q) /CSM/SF/SFG8-B/SFG840Q.Seq.d/                       1130   0.0
SFG479 (SFG479Q) /CSM/SF/SFG4-D/SFG479Q.Seq.d/                       1130   0.0
SFF524 (SFF524Q) /CSM/SF/SFF5-A/SFF524Q.Seq.d/                       1130   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG138757|BG138757.1 EST479199 wild tomato pollen Lycopersicon pennellii cDNA clonecLPP11A1 5' sequence, mRNA sequence.    64   4e-09  2
CF811515|CF811515.2 NA716 cDNA non acclimated Bluecrop library Vaccinium corymbosum cDNA 5', mRNA sequence.    64   4e-07  2
AF352732|AF352732.1 Nicotiana tabacum glutamate decarboxylase isozyme 3 mRNA, complete cds.    56   8e-07  2
CK273593|CK273593.1 EST719671 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADC22 5' end, mRNA sequence.    48   8e-07  2
BP750112|BP750112.1 Nicotiana sylvestris mRNA, expressed in root, clone: R-032_C05.    56   8e-07  2
CN547180|CN547180.1 EST 15169 Ripe Grape Berry LambdaZapII Library Vitis vinifera cDNA clone M5SY00RB007H04 5', mRNA sequence.    62   9e-07  2
CD008053|CD008053.1 VVB082D05_335234 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB082D05 5, mRNA sequence.    62   2e-06  2
CD006712|CD006712.1 VVB066D06_332552 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB066D06 5, mRNA sequence.    62   2e-06  2
CD719214|CD719214.1 VVB158G03_410335 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB158G03 5, mRNA sequence.    62   2e-06  2
CA813481|CA813481.1 CA48LN10IF-E8 CA48LU Vitis vinifera cDNA clone CA48LN10IF-E8 5', mRNA sequence.    62   2e-06  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM178379_1197(FM178379|pid:none) Aliivibrio salmonicida LFI1238 ...   284   1e-75
CP000020_1061(CP000020|pid:none) Vibrio fischeri ES114 chromosom...   281   9e-75
CP001358_45(CP001358|pid:none) Desulfovibrio desulfuricans subsp...   277   1e-73
CP000789_2953(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 ch...   277   1e-73
CP000851_2941(CP000851|pid:none) Shewanella pealeana ATCC 700345...   275   8e-73
CR522870_385(CR522870|pid:none) Desulfotalea psychrophila LSv54 ...   266   3e-70
CP000439_1647(CP000439|pid:none) Francisella tularensis subsp. n...   259   5e-68
AM942759_1390(AM942759|pid:none) Proteus mirabilis strain HI4320...   257   1e-67
CP000608_56(CP000608|pid:none) Francisella tularensis subsp. tul...   256   3e-67
AJ749949_1722(AJ749949|pid:none) Francisella tularensis subsp. t...   253   3e-66

 psg: 0.75  gvh: 0.40  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for CHK244 is nuc

>CHK244Z.Seq
NNNNNNNNNNCAATTCATGTCGATGCAGCAAGTGGTGGTTTCGTTGCACCCTTTCTTCAA
CCAGAAATTGTTTGGGATTTTAGACTACCACGTGTTAAATCAATTAATGGCTCTGGTCAT
AAATTTGGTCTATCTCCATTGGGAGTTGGCTGGGTCGTATGGAGAGGTAAAAATGACATT
CACAAGGATTTGGTATTCGATGTCAACTATCTTGGTGGCAATATGCCAACATTTTCATTG
AATTTCTCACGTCCAGGTGGTCAAATAGTATGCCAGTACTATAACTTTTTGCGCCATGGT
CGTAGTGGATATACCGCAGTTCATCAAGCTTGTCTAGATCATGGTATTTTCATTTCCAAG
CAAGTTAAAAAACACGGTATTTTTGATATTGTTTATGATGGTACTGGTGCACTTCCAGGT
GCATGTTGGAAATTGAAAAAAGATGCAAAAGTAAAATTCAATCTCTTTGACCTTTCAGAT
AGAATGAGAAATAGAGGTTGGCAAATTGCATCCTATACTCTACCAAACTTACCAGATATT
GTAGTTCAAAGAGTATTAATTAGACATGGTTTCAGTCATG