>AFG421 (AFG421Q) /CSM/AF/AFG4-A/AFG421Q.Seq.d/
ATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTA
AATTTAGTGACAAAGAAGTTCAAAGTGATACGAAACATTGGCCATTCAAAGTTATTCCAA
AGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCAC
CAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTG
GTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTC
AAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAAC
CAACTGCTGCTGCTATTGCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTT
TAATTTTCGATTTAGGTGGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTG
TATTTGAAGTTAAAGCCACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATC
GTCTCGTTTCTCACTTTGTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTA
ATCAACGTGCTGTTCGTCGTTTAXXXXXXXXXXGGTGTATTAATTCAAGTTTATGAAGGT
GAACGGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCC
ACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTAT
CTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAA
TGATAAGGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATT
CAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTA
TGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTC
AGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCA
AACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCC
AATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTAGTGGTATGCCAGGTGG
TATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAA
TTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAA
TCAATAAAGCTTGCTCATANA

QVAMNPTNTVFDAKRLIGRKFSDKEVQSDTKHWPFKVIPKDGDKPHIQVEFKGETKVFSP
EEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEP
TAAAIAYGLEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNR
LVSHFVDEFKRKHKKDIMGNQRAVRRL---

---GVLIQVYEGERCYDQR**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*
inw*qtkdynyq**gssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn
*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tlsr
rryatr*wyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksiklahx

Frame A:
iklq*iqpiqylmlnv*lvvnlvtkkfkvirnighsklfqrmvishifklnskvklkfsh
qkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmn
qllllllmv*krkvlvkrif*fsi*vvvhlmflslplkmvylklkpplvili*vvkilii
vsfltllmnlnvnikkilwvinvlfvv---

---GVLIQVYEGERCYDQR**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*
inw*qtkdynyq**gssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn
*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tlsr
rryatr*wyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksiklahx

Frame B:
sscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*sflt
rrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**t
ncccyclwfrkerfw*keyfnfrfrwwyi*cfspyh*rwci*s*shrw*ysfrw*rf**s
srfslc**i*t*t*kryyg*stccssf---

---vy*fkfmkvngamtkdnnllgkfelsgippaprgvpqvevtfdvdangilnvsaedk
stgnkqkititndkgrlskeeiekmvadaekfkqqdeqqkdrvesknklenyaftvknsi
kdekvaakisdsdkstiesetesvlkwlesnqtaekdeyedkmkaleavvnpimsklyqe
ggmpqgsgmpggmsndspkssnnkvdeld*rnfseenisik*is*kiis**nq*slli

Frame C:
QVAMNPTNTVFDAKRLIGRKFSDKEVQSDTKHWPFKVIPKDGDKPHIQVEFKGETKVFSP
EEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEP
TAAAIAYGLEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNR
LVSHFVDEFKRKHKKDIMGNQRAVRRL---

---cinssl*r*tvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlkin
qlvtnkrlqlpmirvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiql
kmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsikk
evchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinkacsx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG421 (AFG421Q) /CSM/AF/AFG4-A/AFG421Q.Seq.d/                       2595   0.0
VHI336 (VHI336Q) /CSM/VH/VHI3-B/VHI336Q.Seq.d/                       1368   0.0
AFO615 (AFO615Q) /CSM/AF/AFO6-A/AFO615Q.Seq.d/                       1368   0.0
VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                       1362   0.0
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1362   0.0
VHO655 (VHO655Q) /CSM/VH/VHO6-C/VHO655Q.Seq.d/                       1362   0.0
VHO461 (VHO461Q) /CSM/VH/VHO4-C/VHO461Q.Seq.d/                       1362   0.0
VHK415 (VHK415Q) /CSM/VH/VHK4-A/VHK415Q.Seq.d/                       1362   0.0
VHJ108 (VHJ108Q) /CSM/VH/VHJ1-A/VHJ108Q.Seq.d/                       1362   0.0
VHE322 (VHE322Q) /CSM/VH/VHE3-A/VHE322Q.Seq.d/                       1362   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1306   0.0    2
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1298   0.0    2
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-164  8
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-157  12
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 105   e-116  10
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          105   e-115  10
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   105   e-115  10
M19753|M19753.1 Plasmodium falciparum heat shock protein (HSP70) homologue mRNA, complete cds.    94   e-105  14
AJ310880|AJ310880.1 Cryptosporidium baileyi partial hsp70 gene for heat shock protein  70.   100   e-104  10
AF221539|AF221539.1 Cryptosporidium baileyi heat shock protein 70 (HSP70) gene, partial cds.   100   e-104  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   410   e-113
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   409   e-112
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   381   e-104
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   378   e-103
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   355   1e-96
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           355   1e-96
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   351   3e-95
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   351   3e-95
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   351   4e-95
EU679412_1(EU679412|pid:none) Tetranychus cinnabarinus heat shoc...   348   3e-94

 psg: 0.78  gvh: 0.27  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.04
 nuc: 0.14  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: Golgi

>> prediction for AFG421 is nuc

>AFG421F.Seq
ATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTA
AATTTAGTGACAAAGAAGTTCAAAGTGATACGAAACATTGGCCATTCAAAGTTATTCCAA
AGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCAC
CAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTG
GTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTC
AAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAAC
CAACTGCTGCTGCTATTGCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTT
TAATTTTCGATTTAGGTGGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTG
TATTTGAAGTTAAAGCCACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATC
GTCTCGTTTCTCACTTTGTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTA
ATCAACGTGCTGTTCGTCGTTTA----------

>AFG421Z.Seq
----------GGTGTATTAATTCAAGTTTATGAAGGTGAACGGTGCTATGACCAAAGATA
ATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCAC
AAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATA
AATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAGGGTCGTCTTTCAAAAG
AAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAA
AAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAA
TTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTG
AAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATG
AAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAG
AAGGAGGTATGCCACAAGGTAGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGA
GTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTA
TCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTGCTCATANA

>AFG421P.Seq
ATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTA
AATTTAGTGACAAAGAAGTTCAAAGTGATACGAAACATTGGCCATTCAAAGTTATTCCAA
AGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCAC
CAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTG
GTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTC
AAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAAC
CAACTGCTGCTGCTATTGCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTT
TAATTTTCGATTTAGGTGGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTG
TATTTGAAGTTAAAGCCACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATC
GTCTCGTTTCTCACTTTGTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTA
ATCAACGTGCTGTTCGTCGTTTA----------GGTGTATTAATTCAAGTTTATGAAGGT
GAACGGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCC
ACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTAT
CTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAA
TGATAAGGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATT
CAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTA
TGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTC
AGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCA
AACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCC
AATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTAGTGGTATGCCAGGTGG
TATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAA
TTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAA
TCAATAAAGCTTGCTCATANA