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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK215
Library CH
(Link to library)
Clone ID CHK215
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11045-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>CHK215 (CHK215Q) /CSM/CH/CHK2-A/CHK215Q.Seq.d/
XXXXXXXXXXTATGGAAATTGATATGGATTTATTTAATAAAATTAGTGAAAAATATAAAG
AAAGTAAAAAGAAACAACAACAAATACAACAACGTGAAAAATTCAAACAAAATGCGCCAG
AAACTCAAAAATCTAAACAAATTAACCAAAACAACAATAATAATAATAATAATATCAACA
ACAACAACAACAACAATAATAATAATAATGGTTCAACCGAAACTAAAGCTGATAAACCAT
CAATGATAAGAAGAAAATCATTATTACCAGTTGATCCTTCAACGATCGCTGCTTTATCTT
TCTCATCGTTCACTTGAAGATATTATGTCAGCAAATAGTAATAGCGGTAATGATGACGAT
GATGAAAATAATCATACCAATCATGATTCTGAAATTGAAAATGAAGTTAAAGAAGATTTT
AGGGTACCTGTAAATAATCGTTACNCCTT
sequence update 2002. 9.10
Translated Amino Acid sequence 
---MEIDMDLFNKISEKYKESKKKQQQIQQREKFKQNAPETQKSKQINQNNNNNNNNINN
NNNNNNNNNGSTETKADKPSMIRRKSLLPVDPSTIAALSFSSFT*ryyvsk***r***r*
*k*syqs*f*n*k*s*rrf*gtck*slxl
Translated Amino Acid sequence (All Frames) 
Frame A:
---MEIDMDLFNKISEKYKESKKKQQQIQQREKFKQNAPETQKSKQINQNNNNNNNNINN
NNNNNNNNNGSTETKADKPSMIRRKSLLPVDPSTIAALSFSSFT*ryyvsk***r***r*
*k*syqs*f*n*k*s*rrf*gtck*slxl


Frame B:
---wkliwiyliklvknikkvkrnnnkynnvknsnkmrqklknlnkltkttiiiiiistt
ttttiiiimvqpklklinhq**eenhyyqlilqrsllylshrsledimsansnsgndddd
ennhtnhdseienevkedfrvpvnnryx


Frame C:
---gn*ygfi**n**ki*rk*ketttnttt*kiqtkcarnski*tn*pkqq*****yqqq
qqqq****wfnrn*s**tindkkkiiits*sfndrcfiflivhlkilcqqiviavmmtmm
kiiipimilklkmklkkilgyl*iivtp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK215 (CHK215Q) /CSM/CH/CHK2-A/CHK215Q.Seq.d/                        470   e-132
AFM407 (AFM407Q) /CSM/AF/AFM4-A/AFM407Q.Seq.d/                        448   e-125
CHP634 (CHP634Q) /CSM/CH/CHP6-B/CHP634Q.Seq.d/                        432   e-120
VHI443 (VHI443Q) /CSM/VH/VHI4-B/VHI443Q.Seq.d/                        426   e-118
CHP174 (CHP174Q) /CSM/CH/CHP1-D/CHP174Q.Seq.d/                        404   e-112
AHQ619 (AHQ619Q) /CSM/AH/AHQ6-A/AHQ619Q.Seq.d/                        398   e-110
AHH552 (AHH552Q) /CSM/AH/AHH5-C/AHH552Q.Seq.d/                        398   e-110
CFF560 (CFF560Q) /CSM/CF/CFF5-C/CFF560Q.Seq.d/                        396   e-109
CHF184 (CHF184Q) /CSM/CH/CHF1-D/CHF184Q.Seq.d/                        392   e-108
AHL419 (AHL419Q) /CSM/AH/AHL4-A/AHL419Q.Seq.d/                        377   e-103
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC144801|AC144801.2 Mus musculus BAC clone RP24-276M2 from chromosome 18, complete sequence.    50   0.033  1
BX161543|BX161543.1 Danio rerio genomic clone DKEY-169L1, genomic survey sequence.    50   0.033  1
AC115683|AC115683.2 Dictyostelium discoideum chromosome 2 map complement(6135149-6105997) strain AX4, complete sequence.    44   0.050  3
AC094391|AC094391.5 Rattus norvegicus clone CH230-3L12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38   0.17   4
AC131635|AC131635.4 Rattus norvegicus clone CH230-10L8, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    42   0.18   3
BX571856|BX571856.1 Staphylococcus aureus subsp. aureus strain MRSA252, complete genome.    36   0.25   17
AC117103|AC117103.3 Rattus norvegicus clone CH230-247C12, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    32   0.27   5
BX571857|BX571857.1 Staphylococcus aureus strain MSSA476, complete genome.    36   0.42   17
BA000033|BA000033.2 Staphylococcus aureus subsp. aureus MW2 DNA, complete genome, strain:MW2.    36   0.46   17
AC128195|AC128195.3 Rattus norvegicus clone CH230-439L12, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    32   0.48   7
dna update 2005. 1.30
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 315,223,790
protein update 2009. 5.19
PSORT 

 psg: 0.75  gvh: 0.06  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for CHK215 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID CHK215Z
3' end seq. 
>CHK215Z.Seq
NNNNNNNNNNTATGGAAATTGATATGGATTTATTTAATAAAATTAGTGAAAAATATAAAG
AAAGTAAAAAGAAACAACAACAAATACAACAACGTGAAAAATTCAAACAAAATGCGCCAG
AAACTCAAAAATCTAAACAAATTAACCAAAACAACAATAATAATAATAATAATATCAACA
ACAACAACAACAACAATAATAATAATAATGGTTCAACCGAAACTAAAGCTGATAAACCAT
CAATGATAAGAAGAAAATCATTATTACCAGTTGATCCTTCAACGATCGCTGCTTTATCTT
TCTCATCGTTCACTTGAAGATATTATGTCAGCAAATAGTAATAGCGGTAATGATGACGAT
GATGAAAATAATCATACCAATCATGATTCTGAAATTGAAAATGAAGTTAAAGAAGATTTT
AGGGTACCTGTAAATAATCGTTACNCCTT
Length of 3' end seq. 449
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -