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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG420
Library AF
(Link to library)
Clone ID AFG420
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16339-1
Original site URL
Representative seq. ID AFG420P
(Link to Original site)
Representative DNA sequence 
>AFG420 (AFG420Q) /CSM/AF/AFG4-A/AFG420Q.Seq.d/
AATCGTAATATTATAATATTTACATATAAAAGAAAAAAAGAAAAAAATTAATTAAAATAA
TTGAAAAATAAAAAAAATGTCATTTAACATACCCACAATTCATACAAATAACTCCAAAAA
CGATAATAATGATGAAAATAATAATAATAATCAACACACAGAAAATCCATACATTTCAAT
AAATAATAAAATCACCCCAGAAAATAGTATCTCATCAAATCTTAATCAAATCGAGAAAGC
TGTTAAAGTTGCCAATTCTCAAAACCTTGATTTAGCATTGATTGGTAATTGCTCATTTGG
TGCTCTTATTGATAAGAAATCATGCATCAAATGGTGTTGCATTCCAAGATTTGATGCAGA
TCCAATATTTTGCTCATTAGTTAGAAAATCAAATGATTCAGGTTTTTATGATGTTGTGGT
TGAAAACTTTGCATTCAGTGAGCAGGTATATATAAAAAACACAGCGGTTTTATCAACAAA
ACTGTACAACACTAATGGAGGTGGTGTTGAAGTAATTGATTTTGCACCAAGATTCAATAT
GTTCTCTCGTCCATACAGACCATCAATGATTATTAGAGTCATTAAGCCATTGGGCCATTG
TAGAATTAAGATTAGACTAAGACCAACATTCGGTTATGGATGGGGTGCACCAGAAAAAAC
CAGAGGTACAAACCATATAAGATATATATTACCATCAATGGTAATGAGACTTXXXXXXXX
XXGAAGGTTTAGGTATGCAAGCATATGCGNATTATAATAAACCAGGATTGTGGTCCATTA
GGTAAAGGTTCAATTGAAGATGGTAAAATTTATACATTTTCATCATTTATGTGTTGGACA
GGTTGTGATAGATTAGCAAAGATTGCAAAGAGTTTAAAGATTCCATTACGTGCACATTTC
TGGCAAGAATCTGCAAATAAAATCAAGGAAGAAATGATGGAACGTTGCTTCAACAAGGAA
AAGAATTCATTTGTATCAACTTGGGATGGAGATGATGTTGATCCTTACCTTTTATTGATT
GCAGAATCTGGATTCACTAAAATTGATGATCCAAAATTCATTGGAACCGTTGAATATTTA
GAGAAAAAACTTAGAAGAGGCAAATACATTATTCAATCACCAGACAGTGTTAGTGCATCA
ACTACATTCACTCTCTACTATATTTCTTGTATTGCTAAAATGGGTAGAAAAGAAGAAGCA
AGAGAACTTTTTGAAAATATCCTTAATGATTGCACTCCATTGGGTTTAATTAGTCAAGAT
GTTAACCTTGAAACCAAAGAAATTTGGGGTAACTTTCCAAAAACAACTTCAATGGTTGGT
ATAATTAATGCTGCT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
ivil*ylhikekkkkin*nn*KIKKMSFNIPTIHTNNSKNDNNDENNNNNQHTENPYISI
NNKITPENSISSNLNQIEKAVKVANSQNLDLALIGNCSFGALIDKKSCIKWCCIPRFDAD
PIFCSLVRKSNDSGFYDVVVENFAFSEQVYIKNTAVLSTKLYNTNGGGVEVIDFAPRFNM
FSRPYRPSMIIRVIKPLGHCRIKIRLRPTFGYGWGAPEKTRGTNHIRYILPSMVMRL---

---EGLGMQAYAXYNKPGLWSIR*rfn*rw*nlyifiiyvldrl**iskdckefkdsitc
tflarick*nqgrndgtllqqgkeficinlgwr*c*slpfidcriwih*n**skihwnr*
ifrekt*krqihysitrqc*cinyihsllyflyc*ng*krrskrtf*kyp**lhsigfn*
src*p*nqrnlg*lsknnfngwyn*cc
Translated Amino Acid sequence (All Frames) 
Frame A:
nrniiiftykrkkekn*lk*lknkknvi*hthnsyk*lqkr****k****sthrksihfn
k**nhprk*yliks*snresc*scqfskp*fsidw*lliwcsy**eimhqmvlhski*cr
snillis*kik*frfl*ccg*klciq*agiykkhsgfinktvqh*wrwc*sn*fctkiqy
vlssiqtindy*sh*aigpl*n*d*tktnirlwmgctrknqrykpykiyitingnet---

---EGLGMQAYAXYNKPGLWSIR*rfn*rw*nlyifiiyvldrl**iskdckefkdsitc
tflarick*nqgrndgtllqqgkeficinlgwr*c*slpfidcriwih*n**skihwnr*
ifrekt*krqihysitrqc*cinyihsllyflyc*ng*krrskrtf*kyp**lhsigfn*
src*p*nqrnlg*lsknnfngwyn*cc

Frame B:
ivil*ylhikekkkkin*nn*KIKKMSFNIPTIHTNNSKNDNNDENNNNNQHTENPYISI
NNKITPENSISSNLNQIEKAVKVANSQNLDLALIGNCSFGALIDKKSCIKWCCIPRFDAD
PIFCSLVRKSNDSGFYDVVVENFAFSEQVYIKNTAVLSTKLYNTNGGGVEVIDFAPRFNM
FSRPYRPSMIIRVIKPLGHCRIKIRLRPTFGYGWGAPEKTRGTNHIRYILPSMVMRL---

---kv*vckhmriiinqdcgplgkgsiedgkiytfssfmcwtgcdrlakiakslkiplra
hfwqesankikeemmercfnkeknsfvstwdgddvdpyllliaesgftkiddpkfigtve
ylekklrrgkyiiqspdsvsasttftlyyisciakmgrkeearelfenilndctplglis
qdvnletkeiwgnfpkttsmvgiinaa

Frame C:
s*yyniyi*kkkrkklikiiek*kkchltypqfiqitpktiimmkiiiiintqkihtfq*
iikspqkivshqiliksrkllklpilktli*h*lviahlvlllirnhasngvafqdlmqi
qyfah*lenqmiqvfmmlwlktlhsvsryi*ktqrfyqqncttlmevvlk*lilhqdsic
slvhtdhq*lleslshwaivelrld*dqhsvmdgvhqkkpevqti*diyyhqw**d---

---rfryasicxl**trivvh*vkvqlkmvkfihfhhlcvgqvvid*qrlqrv*rfhyvh
isgknlqiksrkk*wnvastrkrihlyqlgmemmliltfy*lqnldslklmiqnslepln
i*rknleeantlfnhqtvlvhqlhslstiflvllkwvekkkqenflkislmialhwv*lv
kmltlkpkkfgvtfqkqlqwlv*lml
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG420 (AFG420Q) /CSM/AF/AFG4-A/AFG420Q.Seq.d/                       2428   0.0
AHE881 (AHE881Q) /CSM/AH/AHE8-D/AHE881Q.Seq.d/                       1178   0.0
CFE768 (CFE768Q) /CSM/CF/CFE7-C/CFE768Q.Seq.d/                       1174   0.0
AFA132 (AFA132Q) /CSM/AF/AFA1-B/AFA132Q.Seq.d/                       1174   0.0
CFI825 (CFI825Q) /CSM/CF/CFI8-B/CFI825Q.Seq.d/                       1170   0.0
SFC769 (SFC769Q) /CSM/SF/SFC7-C/SFC769Q.Seq.d/                       1164   0.0
CHC496 (CHC496Q) /CSM/CH/CHC4-D/CHC496Q.Seq.d/                       1162   0.0
CHB367 (CHB367Q) /CSM/CH/CHB3-C/CHB367Q.Seq.d/                       1162   0.0
CFJ243 (CFJ243Q) /CSM/CF/CFJ2-B/CFJ243Q.Seq.d/                       1162   0.0
AHF801 (AHF801Q) /CSM/AH/AHF8-A/AHF801Q.Seq.d/                       1162   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1130   0.0    5
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           56   9e-09  6
AE014833|AE014833.1 Plasmodium falciparum 3D7 chromosome 10 section 5 of 7 of the complete sequence.    46   2e-08  15
AC115606|AC115606.2 Dictyostelium discoideum chromosome 2 map 159760-209066 strain AX4, complete sequence.    44   4e-08  9
AC004709|AC004709.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    50   6e-08  13
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    50   6e-08  11
AE014820|AE014820.1 Plasmodium falciparum 3D7 chromosome 14 section 5 of 13 of the complete sequence.    48   7e-08  10
AL929358|AL929358.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 4/5.    50   7e-08  14
AE014840|AE014840.1 Plasmodium falciparum 3D7 chromosome 11 section 5 of 8 of the complete sequence.    44   2e-07  14
CR382399|CR382399.1 Plasmodium falciparum chromosome 6, complete sequence; segment 2/5.    52   2e-07  6
dna update 2004. 5. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116979_62(AC116979|pid:none) Dictyostelium discoideum chromoso...   392   e-110
AY923753_1(AY923753|pid:none) Physarum polycephalum glycosyl hyd...   233   1e-59
DQ384332_1(DQ384332|pid:none) Physarum polycephalum glycosyl hyd...   231   3e-59
DQ390347_1(DQ390347|pid:none) Hartmannella vermiformis glycosyl ...   183   1e-44
DQ384328_1(DQ384328|pid:none) Hartmannella vermiformis glycosyl ...   181   4e-44
DQ384329_1(DQ384329|pid:none) Hartmannella vermiformis glycosyl ...   181   5e-44
AY924416_1(AY924416|pid:none) Acanthamoeba castellanii glycosyl ...   174   5e-42
DQ384325_1(DQ384325|pid:none) Acanthamoeba castellanii NADPH:qui...   169   2e-40
AM039952_3335(AM039952|pid:none) Xanthomonas campestris pv. vesi...   159   3e-37
AE008923_3162(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   158   5e-37
protein update 2009. 4.20
PSORT 

 psg: 0.86  gvh: 0.27  alm: 0.44  top: 0.17  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFG420 is cyt
5' end seq. ID AFG420F
5' end seq. 
>AFG420F.Seq
AATCGTAATATTATAATATTTACATATAAAAGAAAAAAAGAAAAAAATTAATTAAAATAA
TTGAAAAATAAAAAAAATGTCATTTAACATACCCACAATTCATACAAATAACTCCAAAAA
CGATAATAATGATGAAAATAATAATAATAATCAACACACAGAAAATCCATACATTTCAAT
AAATAATAAAATCACCCCAGAAAATAGTATCTCATCAAATCTTAATCAAATCGAGAAAGC
TGTTAAAGTTGCCAATTCTCAAAACCTTGATTTAGCATTGATTGGTAATTGCTCATTTGG
TGCTCTTATTGATAAGAAATCATGCATCAAATGGTGTTGCATTCCAAGATTTGATGCAGA
TCCAATATTTTGCTCATTAGTTAGAAAATCAAATGATTCAGGTTTTTATGATGTTGTGGT
TGAAAACTTTGCATTCAGTGAGCAGGTATATATAAAAAACACAGCGGTTTTATCAACAAA
ACTGTACAACACTAATGGAGGTGGTGTTGAAGTAATTGATTTTGCACCAAGATTCAATAT
GTTCTCTCGTCCATACAGACCATCAATGATTATTAGAGTCATTAAGCCATTGGGCCATTG
TAGAATTAAGATTAGACTAAGACCAACATTCGGTTATGGATGGGGTGCACCAGAAAAAAC
CAGAGGTACAAACCATATAAGATATATATTACCATCAATGGTAATGAGACTT--------
--
Length of 5' end seq. 712
3' end seq. ID AFG420Z
3' end seq. 
>AFG420Z.Seq
----------GAAGGTTTAGGTATGCAAGCATATGCGNATTATAATAAACCAGGATTGTG
GTCCATTAGGTAAAGGTTCAATTGAAGATGGTAAAATTTATACATTTTCATCATTTATGT
GTTGGACAGGTTGTGATAGATTAGCAAAGATTGCAAAGAGTTTAAAGATTCCATTACGTG
CACATTTCTGGCAAGAATCTGCAAATAAAATCAAGGAAGAAATGATGGAACGTTGCTTCA
ACAAGGAAAAGAATTCATTTGTATCAACTTGGGATGGAGATGATGTTGATCCTTACCTTT
TATTGATTGCAGAATCTGGATTCACTAAAATTGATGATCCAAAATTCATTGGAACCGTTG
AATATTTAGAGAAAAAACTTAGAAGAGGCAAATACATTATTCAATCACCAGACAGTGTTA
GTGCATCAACTACATTCACTCTCTACTATATTTCTTGTATTGCTAAAATGGGTAGAAAAG
AAGAAGCAAGAGAACTTTTTGAAAATATCCTTAATGATTGCACTCCATTGGGTTTAATTA
GTCAAGATGTTAACCTTGAAACCAAAGAAATTTGGGGTAACTTTCCAAAAACAACTTCAA
TGGTTGGTATAATTAATGCTGCT
Length of 3' end seq. 613
Connected seq. ID AFG420P
Connected seq. 
>AFG420P.Seq
AATCGTAATATTATAATATTTACATATAAAAGAAAAAAAGAAAAAAATTAATTAAAATAA
TTGAAAAATAAAAAAAATGTCATTTAACATACCCACAATTCATACAAATAACTCCAAAAA
CGATAATAATGATGAAAATAATAATAATAATCAACACACAGAAAATCCATACATTTCAAT
AAATAATAAAATCACCCCAGAAAATAGTATCTCATCAAATCTTAATCAAATCGAGAAAGC
TGTTAAAGTTGCCAATTCTCAAAACCTTGATTTAGCATTGATTGGTAATTGCTCATTTGG
TGCTCTTATTGATAAGAAATCATGCATCAAATGGTGTTGCATTCCAAGATTTGATGCAGA
TCCAATATTTTGCTCATTAGTTAGAAAATCAAATGATTCAGGTTTTTATGATGTTGTGGT
TGAAAACTTTGCATTCAGTGAGCAGGTATATATAAAAAACACAGCGGTTTTATCAACAAA
ACTGTACAACACTAATGGAGGTGGTGTTGAAGTAATTGATTTTGCACCAAGATTCAATAT
GTTCTCTCGTCCATACAGACCATCAATGATTATTAGAGTCATTAAGCCATTGGGCCATTG
TAGAATTAAGATTAGACTAAGACCAACATTCGGTTATGGATGGGGTGCACCAGAAAAAAC
CAGAGGTACAAACCATATAAGATATATATTACCATCAATGGTAATGAGACTT--------
--GAAGGTTTAGGTATGCAAGCATATGCGNATTATAATAAACCAGGATTGTGGTCCATTA
GGTAAAGGTTCAATTGAAGATGGTAAAATTTATACATTTTCATCATTTATGTGTTGGACA
GGTTGTGATAGATTAGCAAAGATTGCAAAGAGTTTAAAGATTCCATTACGTGCACATTTC
TGGCAAGAATCTGCAAATAAAATCAAGGAAGAAATGATGGAACGTTGCTTCAACAAGGAA
AAGAATTCATTTGTATCAACTTGGGATGGAGATGATGTTGATCCTTACCTTTTATTGATT
GCAGAATCTGGATTCACTAAAATTGATGATCCAAAATTCATTGGAACCGTTGAATATTTA
GAGAAAAAACTTAGAAGAGGCAAATACATTATTCAATCACCAGACAGTGTTAGTGCATCA
ACTACATTCACTCTCTACTATATTTCTTGTATTGCTAAAATGGGTAGAAAAGAAGAAGCA
AGAGAACTTTTTGAAAATATCCTTAATGATTGCACTCCATTGGGTTTAATTAGTCAAGAT
GTTAACCTTGAAACCAAAGAAATTTGGGGTAACTTTCCAAAAACAACTTCAATGGTTGGT
ATAATTAATGCTGCT
Length of connected seq. 1325
Full length Seq ID -
Full length Seq. -
Length of full length seq. -