>CHK177 (CHK177Q) /CSM/CH/CHK1-D/CHK177Q.Seq.d/
ATATTTATATTTTATAAATATATATTTTTAATATGGCACATATTGATCGTATTAATATAC
TTGCCAATCATCTTTCAAAGGAAGAAGAAGAAGAAAATGAATTATTAACTTCACCAGTTT
CTGCTGATAAAAAAAAAGAGACAGTTACAGTTACAGATAATAGAAATGGTAAAAGCTATG
ATTTCAAAATTAAAAATGACACTATTAATGCTTTAAACTTTAAAGAATTACAATTAGCCA
AAGGTGATGGTGGTTTAATGATTTATGATCCAGGTTTCCAAAATACAGCAGTTGTAACAT
CATACATTACATATATTGATGGTGATAAAGGAATTTTAAGATACAGAGGTTATCCAATTG
AAGAATTAGCAGAAAGATCAAACTTTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACT
TACCAAATAAATCACAATTAGATGGTTGGAGTAATAAAATTATGACTCATACTTTTCTTC
ATGAAAATTTGGXXXXXXXXXXGATATGTTAATTCATATCGGTTCCAAAGAGAATATTCC
ACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAAT
CTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGA
AAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGA
AGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTCAT
CTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGTGC
CGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTATCG
TCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCCAATCGATGGTAGACC
ACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAA

iyil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETVTVTDNRNGKSYD
FKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGDKGILRYRGYPIE
ELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENL---

---DMLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGK
EPLIEVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWL
AHWVEHLEDPETKIYRPRQVYKGEWFRNYVPIDGRPPAKVRSQDSYSSATTK

Frame A:
ififykyifliwhilivliylpiifqrkkkkkmny*lhqfllikkkrqlqlqiiemvkam
isklkmtllml*tlknyn*pkvmvv**fmiqvskiqql*hhtlhilmvikef*dteviql
kn*qkdqtf*rllif*smvtyqinhn*mvgvikl*lilffmkiw---

---DMLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGK
EPLIEVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWL
AHWVEHLEDPETKIYRPRQVYKGEWFRNYVPIDGRPPAKVRSQDSYSSATTK

Frame B:
ylyfiniyf*ygty*sy*ytcqssfkgrrrrk*iinftsfc**kkrdsysyr**kw*kl*
fqn*k*hy*cfkl*ritisqr*wwfndl*srfpkysscniihyiy*w**rnfkiqrlsn*
risrkiklfrgclsfnqw*ltk*itirwle**nydsyfss*kf---

---ic*fisvpkrifhnsfqm*rakrrn*wvsviestktmiqeprsfve*lmkfskv*vk
nh*lk*plswrnkpwkmntl*aenfiqmlistlvssikqwvsqlicsqfyslfhvplvg*
lig*ni*kiqklksivhvkftkvngletmsqsmvdhqlkfvlrivirllplk

Frame C:
iyil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETVTVTDNRNGKSYD
FKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGDKGILRYRGYPIE
ELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENL---

---yvnsyrfqreystihfrckeqkeeingfrs*nlqkl*sksqdhs*ssl*sfrkfr*r
tid*ssh*vgetslgr*ilckqktlskc*fllwshl*sngfpn*yvpsfihystcrwlvs
slgrtfrrsrn*nlsstsslqr*mv*klcpnrw*tts*ssfsg*lfvcyh*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK177 (CHK177Q) /CSM/CH/CHK1-D/CHK177Q.Seq.d/                       1772   0.0
SSG238 (SSG238Q) /CSM/SS/SSG2-B/SSG238Q.Seq.d/                       1007   0.0
SSF821 (SSF821Q) /CSM/SS/SSF8-A/SSF821Q.Seq.d/                       1007   0.0
SFE393 (SFE393Q) /CSM/SF/SFE3-D/SFE393Q.Seq.d/                       1007   0.0
AFN717 (AFN717Q) /CSM/AF/AFN7-A/AFN717Q.Seq.d/                       1007   0.0
AFM803 (AFM803Q) /CSM/AF/AFM8-A/AFM803Q.Seq.d/                       1007   0.0
AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/                       1007   0.0
AFC579 (AFC579Q) /CSM/AF/AFC5-D/AFC579Q.Seq.d/                       1007   0.0
SSK530 (SSK530Q) /CSM/SS/SSK5-B/SSK530Q.Seq.d/                        999   0.0
AFJ723 (AFJ723Q) /CSM/AF/AFJ7-A/AFJ723Q.Seq.d/                        991   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY124377|AY124377.1 Dictyostelium discoideum citrate synthase (cshA) mRNA, complete cds.  1007   0.0    3
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    80   2e-41  11
AB109907|AB109907.1 Tetrahymena thermophila tgCS mRNA for putative citrate synthase, complete cds.   101   5e-31  5
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    76   3e-21  2
AL385088|AL385088.1 Medicago truncatula EST MtBC26C06F1 : T3 end of clone MtBC26C06 of  cDNA library MtBC from arbuscular mycorrhiza of cultivar Jemalong of Medicago truncatula (barrel medic).   103   3e-21  2
Z70012|Z70012.1 Bartonella sp. gltA gene (strain N40).             52   3e-12  3
CV049517|CV049517.1 EST 14834 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bgh001o07q 5', mRNA sequence.    62   4e-10  2
CV052502|CV052502.1 EST 11950 Half-Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bge016g02q 5', mRNA sequence.    62   4e-10  2
BU044708|BU044708.1 PP_LEa0020E04f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0020E04f, mRNA sequence.    62   4e-10  2
BU040616|BU040616.1 PP_LEa0006L02f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0006L02f, mRNA sequence.    62   5e-10  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB109907_1(AB109907|pid:none) Tetrahymena thermophila tgCS mRNA ...   233   6e-60
AK120755_1(AK120755|pid:none) Oryza sativa Japonica Group cDNA c...   232   1e-59
AF310892_1(AF310892|pid:none) Dictyostelium discoideum citrate s...   230   6e-59
AB167269_1(AB167269|pid:none) Chlorobium limicola cit gene for c...   224   5e-57
CP001099_363(CP001099|pid:none) Chlorobaculum parvum NCIB 8327, ...   224   5e-57
(P49299) RecName: Full=Citrate synthase, glyoxysomal;          E...   222   1e-56
CP001110_2246(CP001110|pid:none) Pelodictyon phaeoclathratiforme...   220   5e-56
CP000096_332(CP000096|pid:none) Pelodictyon luteolum DSM 273, co...   220   5e-56
CP000607_394(CP000607|pid:none) Chlorobium phaeovibrioides DSM 2...   220   7e-56
CR954217_232(CR954217|pid:none) Ostreococcus tauri strain OTTH05...   218   2e-55

 psg: 0.76  gvh: 0.30  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for CHK177 is nuc

>CHK177F.Seq
ATATTTATATTTTATAAATATATATTTTTAATATGGCACATATTGATCGTATTAATATAC
TTGCCAATCATCTTTCAAAGGAAGAAGAAGAAGAAAATGAATTATTAACTTCACCAGTTT
CTGCTGATAAAAAAAAAGAGACAGTTACAGTTACAGATAATAGAAATGGTAAAAGCTATG
ATTTCAAAATTAAAAATGACACTATTAATGCTTTAAACTTTAAAGAATTACAATTAGCCA
AAGGTGATGGTGGTTTAATGATTTATGATCCAGGTTTCCAAAATACAGCAGTTGTAACAT
CATACATTACATATATTGATGGTGATAAAGGAATTTTAAGATACAGAGGTTATCCAATTG
AAGAATTAGCAGAAAGATCAAACTTTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACT
TACCAAATAAATCACAATTAGATGGTTGGAGTAATAAAATTATGACTCATACTTTTCTTC
ATGAAAATTTGGNNNNNNNNNN

>CHK177Z.Seq
NNNNNNNNNNGATATGTTAATTCATATCGGTTCCAAAGAGAATATTCCACAATTCATTTC
AGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAATCTACAAAAACTA
TGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGAAAGTTTAGGTAA
AGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGAAGATGAATACTT
TGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTCATCTATAAAGCAAT
GGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGTGCCGTTGGTTGGTT
AGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTATCGTCCACGTCAAGT
TTACAAAGGTGAATGGTTTAGAAACTATGTCCCAATCGATGGTAGACCACCAGCTAAAGT
TCGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAA

>CHK177P.Seq
ATATTTATATTTTATAAATATATATTTTTAATATGGCACATATTGATCGTATTAATATAC
TTGCCAATCATCTTTCAAAGGAAGAAGAAGAAGAAAATGAATTATTAACTTCACCAGTTT
CTGCTGATAAAAAAAAAGAGACAGTTACAGTTACAGATAATAGAAATGGTAAAAGCTATG
ATTTCAAAATTAAAAATGACACTATTAATGCTTTAAACTTTAAAGAATTACAATTAGCCA
AAGGTGATGGTGGTTTAATGATTTATGATCCAGGTTTCCAAAATACAGCAGTTGTAACAT
CATACATTACATATATTGATGGTGATAAAGGAATTTTAAGATACAGAGGTTATCCAATTG
AAGAATTAGCAGAAAGATCAAACTTTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACT
TACCAAATAAATCACAATTAGATGGTTGGAGTAATAAAATTATGACTCATACTTTTCTTC
ATGAAAATTTGG----------GATATGTTAATTCATATCGGTTCCAAAGAGAATATTCC
ACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAAT
CTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGA
AAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGA
AGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTCAT
CTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGTGC
CGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTATCG
TCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCCAATCGATGGTAGACC
ACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAA