NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHK116
Library CH
(Link to library)
Clone ID CHK116
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11302-1
Original site URL
Representative seq. ID CHK116P
(Link to Original site)
Representative DNA sequence 
>CHK116 (CHK116Q) /CSM/CH/CHK1-A/CHK116Q.Seq.d/
AATAAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATA
TATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTA
TGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAA
GTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAA
GAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAA
TTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCC
CACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAAC
AAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAG
AGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAAC
CACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACATAATGTAX
XXXXXXXXXTATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGT
CGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACA
ACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGAT
TTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCC
GATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGAC
CCTTCAGTACTAGGTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACC
TCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCA
ACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAAT
CCTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGT
GGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATAT
GATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAATAAATAAAATAATAAAT
AAATTTTAAAATATA
sequence update 2002.10.25
Translated Amino Acid sequence 
*ttnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNFEQS
NVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVKVCP
PDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLSNKP
PEESLVNYYLSNIAKKHNV---

---YEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAEREIVRDIKEKTSFVSQD
FYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLGKDQNGIHHLLNETIT
SCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQIKINAPPNRDISVWCG
GSVLGDLKTFSDQWITKQEYDEVGRGIVHRKCF*inkiinkf*ni
Translated Amino Acid sequence (All Frames) 
Frame A:
nklqiwmln*l*fvnitfciyiyiknkklk*qdfqfli*vcysn*qivdwyyikikilnk
vmfikkrlqnyyn*vkknkqelerfqllmkiitlkf*vf*lfivkl*qieleelkv*rya
hqi*kkhvvvlylhhhi*inklnyirlerd*lknlvknsqrnv*ivvvsiqrlyidyqin
hlknhw*tiilvilqrnim---

---YEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAEREIVRDIKEKTSFVSQD
FYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLGKDQNGIHHLLNETIT
SCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQIKINAPPNRDISVWCG
GSVLGDLKTFSDQWITKQEYDEVGRGIVHRKCF*inkiinkf*ni

Frame B:
inykfgc*inynlli*hfvyiyi*kiknsndrifns*yeyvtqisk**igit*k*kf*tk
*cs*krdcriitir*rrtsks*ngfsy**rlsh*sfryfnyll*nfsk*n*rn*rckgmp
trfkrsml*yyicitifr*ts*ii*d*kein*kiw*kiprgmyrlllyqskdct*iik*t
t*riigklls**ycket*c---

---mkvilfhmqlkesisvvvisqsi*cvyslkevmlslqppkeksyviskrklhlslki
smpl*mlqriiiysriircpinkf*plvkkdsvvmklsltlqy*vkikmvfits*mklsp
lvilifvvisikil*flvvqqwqqvlknvykkkwnf*fqilksklklmhhqivilvfgvv
vrf*vilklfringlqnknmmklveelfienvfk*ik**infki

Frame C:
*ttnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNFEQS
NVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVKVCP
PDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLSNKP
PEESLVNYYLSNIAKKHNV---

---*rlfystcn*knryrws*yhrvfnafth*krlcfhynrrkrnrt*yqrenficlsrf
lclyecsrg**ytqglydar*tsfnhw*rkipll*sfl*pfstr*rskwyssplk*nyhl
l*y*yss*sl*kycsfwwfnngnrf*ktftkrngtfsfkssspn*n*cttks*y*clvww
fgfr*s*nffgsmdyktri**sw*rncs*kmflnk*nnk*ilky
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHK116 (CHK116Q) /CSM/CH/CHK1-A/CHK116Q.Seq.d/                       2222   0.0
VHD820 (VHD820Q) /CSM/VH/VHD8-A/VHD820Q.Seq.d/                       1241   0.0
AHL385 (AHL385Q) /CSM/AH/AHL3-D/AHL385Q.Seq.d/                       1241   0.0
AHF512 (AHF512Q) /CSM/AH/AHF5-A/AHF512Q.Seq.d/                       1241   0.0
AHE579 (AHE579Q) /CSM/AH/AHE5-D/AHE579Q.Seq.d/                       1241   0.0
AHP667 (AHP667Q) /CSM/AH/AHP6-C/AHP667Q.Seq.d/                       1239   0.0
SHC311 (SHC311Q) /CSM/SH/SHC3-A/SHC311Q.Seq.d/                       1237   0.0
CHF491 (CHF491Q) /CSM/CH/CHF4-D/CHF491Q.Seq.d/                       1233   0.0
SHH289 (SHH289Q) /CSM/SH/SHH2-D/SHH289Q.Seq.d/                       1225   0.0
AHM227 (AHM227Q) /CSM/AH/AHM2-B/AHM227Q.Seq.d/                       1225   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X52604|X52604.1 Artemia mRNA for actin (clone pArAct302).          66   3e-10  2
AJ269585|AJ269585.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-7.    66   3e-10  2
AJ269586|AJ269586.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-14.    66   3e-10  2
AJ269584|AJ269584.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-6.    66   3e-10  2
AJ269580|AJ269580.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-13.    66   3e-10  2
AJ269583|AJ269583.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-3.    66   3e-10  2
AJ269578|AJ269578.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-11.    66   3e-10  2
AJ269574|AJ269574.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-17.    66   3e-10  2
AJ269577|AJ269577.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-19.    66   3e-10  2
AJ269573|AJ269573.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-12.    66   3e-10  2
dna update 2005. 1.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11426) RecName: Full=Actin;  &ATAXE(A29877;A54526;S33463) &M16...   256   9e-67
AM411663_1(AM411663|pid:none) Plasmodiophora brassicae actII gen...   256   2e-66
AY294156_1(AY294156|pid:none) Pelomyxa palustris actin mRNA, com...   254   3e-66
(P53458) RecName: Full=Actin-5; Flags: Fragment;  &U27836_1(U278...   254   3e-66
(P18600) RecName: Full=Actin, clone 205;  &S11450(S11450) &X5260...   254   4e-66
(O17320) RecName: Full=Actin;  &AF026063_1(AF026063|pid:none)         254   4e-66
(P45886) RecName: Full=Actin-3, muscle-specific;  &L12255_1(L122...   254   4e-66
AB036756_1(AB036756|pid:none) Pagrus major mRNA for B-actin, com...   254   6e-66
AB086826_1(AB086826|pid:none) Galaxea fascicularis LGfact mRNA f...   254   6e-66
AY335441_1(AY335441|pid:none) Chlamys farreri actin mRNA, comple...   253   7e-66
protein update 2009. 4. 3
PSORT 

 psg: 0.87  gvh: 0.48  alm: 0.41  top: 0.70  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: peroxisomal

>> prediction for CHK116 is mit
5' end seq. ID CHK116F
5' end seq. 
>CHK116F.Seq
AATAAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATA
TATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTA
TGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAA
GTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAA
GAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAA
TTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCC
CACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAAC
AAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAG
AGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAAC
CACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACATAATGTAN
NNNNNNNNN
Length of 5' end seq. 609
3' end seq. ID CHK116Z
3' end seq. 
>CHK116Z.Seq
NNNNNNNNNNTATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGG
TCGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTAC
AACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGA
TTTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCC
CGATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGA
CCCTTCAGTACTAGGTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCAC
CTCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTC
AACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAA
TCCTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGG
TGGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATA
TGATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAATAAATAAAATAATAAA
TAAATTTTAAAATATA
Length of 3' end seq. 676
Connected seq. ID CHK116P
Connected seq. 
>CHK116P.Seq
AATAAACTACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACATTTTGTATA
TATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAATATGAGTA
TGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTTGAACAAA
GTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAACAAGCAA
GAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGTATTTTAA
TTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAGGTATGCC
CACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTAGATAAAC
AAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAATTCCCAG
AGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCAAATAAAC
CACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACATAATGTA-
---------TATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGT
CGTGATATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACA
ACCGCCGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGAT
TTCTATGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCC
GATAAACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGAC
CCTTCAGTACTAGGTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACC
TCTTGTGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCA
ACAATGGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAAT
CCTCAAGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGT
GGTTCGGTTTTAGGTGATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATAT
GATGAAGTTGGTAGAGGAATTGTTCATAGAAAATGTTTTTAAATAAATAAAATAATAAAT
AAATTTTAAAATATA
Length of connected seq. 1265
Full length Seq ID -
Full length Seq. -
Length of full length seq. -