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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG366
Library AF
(Link to library)
Clone ID AFG366
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16173-1
Original site URL
Representative seq. ID AFG366P
(Link to Original site)
Representative DNA sequence 
>AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/
AAAAAAAAAAGAACAGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTT
ATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTG
ATCGTATCAGNGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAA
ATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTG
GATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATC
CAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTA
CAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCAC
GTAAAGTACATCCAACAGTTATTATTAGNGGTTTTAGATTAXXXXXXXXXXTAAACGTAT
CGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATC
ATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGA
TGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGC
CAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAA
GAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTC
TATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGA
ATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGC
CTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAA
GAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGA
GGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAG
CGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAATCCAAGAGAACAACAAGG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
kkknr*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRIXGNEVRAQNVLAVTAIAN
IVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGT
TTVVILAAELLKRANELVARKVHPTVIIXGFRL---

---KRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKSAS
IILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGSRK
QLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNK
VRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQ
Translated Amino Acid sequence (All Frames) 
Frame A:
kkkrtdslhqtifiyiqyifiy*ikilkkecqtry**lmvivsxvmklehkmf*qlqqlq
i*lkhhldqlv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvmv
qlql*f*qqny*nvpmnw*hvkyiqqlllxvld---

---*tyrqimwwyslnhtcksrg*rii*yhrlgycr*scsr*igr**inhcqklk*eisf
nhsswcq*invg*ngtfhs*lslyrkenfriwnhcsrwwcs*icplylsr*ycrhng*s*
tisnl*ic*iplgctktigckccs*cl*fsfkikslsscrsn*sikeilrilwsrfiq*q
s*k*frgwcfrtchcqnqmyqicn*kryhhpsyr**nhs*skrttr

Frame B:
kkkeqivstkrylytsniflyielkf*kknvkqgind*w*syqx**s*stkcfssysnck
ys*niiwtnwfr*nvn**ywinccyq*wcnhftkd*yrtssskdfstiiriarsrsw*wy
nyscnfssriietcq*igst*stsnsyy*xf*i---

---KRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKSAS
IILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGSRK
QLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFNNK
VRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQ

Frame C:
kkknr*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRIXGNEVRAQNVLAVTAIAN
IVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGT
TTVVILAAELLKRANELVARKVHPTVIIXGFRL---

---nvspnhvvvqs*shlqisrvknhlipppwvlpmklfkidwpmmn*slsktqirnqlq
sffvvpmn*cwmkwnvpfmtlsvs*rel*nleplfqvvvqlnlpslsisiilppqwvvvn
n*qslnllnpswlyqnnwl*mlllmpli*fqn*kpiimplklihqrnlthtlvsiysitk
leii*rlvf*nlplpksnvsnlqlkalspsfvsmikslliqennk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                       2127   0.0
CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                       1326   0.0
AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/                       1326   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                       1326   0.0
VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                       1318   0.0
VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                       1251   0.0
VFA730 (VFA730Q) /CSM/VF/VFA7-B/VFA730Q.Seq.d/                       1221   0.0
VFL307 (VFL307Q) /CSM/VF/VFL3-A/VFL307Q.Seq.d/                       1209   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                     1183   0.0
FC-116 (FC-116Q) /CSM/CG/CG-FC/FC-116Q.Seq.d/                        1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.  1326   0.0    2
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66   3e-16  3
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70   4e-14  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68   2e-12  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   2e-12  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   2e-12  2
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48   4e-10  4
CN099114|CN099114.1 EC2CAA15CG11.b1 Xenopus tropicalis xthr plasmid library Xenopus tropicalis cDNA clone xthr15N21 3', mRNA sequence.    70   8e-08  1
U43123|U43123.1 Naegleria fowleri T-complex polypeptide homolog mRNA, partial cds.    38   1e-07  4
AF143494|AF143494.1 Xenopus laevis t-complex polypeptide 1 (TCP1) mRNA, complete cds.    48   4e-07  4
dna update 2004. 5. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   422   e-117
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   321   3e-86
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   285   2e-75
(P18279) RecName: Full=T-complex protein 1 subunit alpha;       ...   285   2e-75
AY891878_1(AY891878|pid:none) Synthetic construct Homo sapiens c...   284   3e-75
(Q4R5G2) RecName: Full=T-complex protein 1 subunit alpha;       ...   284   3e-75
(P28480) RecName: Full=T-complex protein 1 subunit alpha;       ...   283   7e-75
AF143494_1(AF143494|pid:none) Xenopus laevis t-complex polypepti...   282   1e-74
(Q32L40) RecName: Full=T-complex protein 1 subunit alpha;       ...   282   1e-74
BC068901_1(BC068901|pid:none) Xenopus laevis t-complex polypepti...   281   3e-74
protein update 2009. 4.20
PSORT 

 psg: 0.57  gvh: 0.40  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFG366 is cyt
5' end seq. ID AFG366F
5' end seq. 
>AFG366F.Seq
AAAAAAAAAAGAACAGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTT
ATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTG
ATCGTATCAGNGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAA
ATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTG
GATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATC
CAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTA
CAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCAC
GTAAAGTACATCCAACAGTTATTATTAGNGGTTTTAGATTA----------
Length of 5' end seq. 461
3' end seq. ID AFG366Z
3' end seq. 
>AFG366Z.Seq
----------TAAACGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAA
ATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTC
AAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTT
CAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATG
ACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTG
CAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTA
AACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTG
TAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCG
CTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACA
AAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTA
TCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTA
ATCCAAGAGAACAACAAGG
Length of 3' end seq. 669
Connected seq. ID AFG366P
Connected seq. 
>AFG366P.Seq
AAAAAAAAAAGAACAGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTT
ATATATTGAATTAAAATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTG
ATCGTATCAGNGGTAATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAA
ATATAGTTAAAACATCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTG
GATCAATTGTTGTTACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATC
CAGCAGCAAAGATTTTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTA
CAACTACAGTTGTAATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCAC
GTAAAGTACATCCAACAGTTATTATTAGNGGTTTTAGATTA----------TAAACGTAT
CGCCAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATC
ATTTGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGA
TGAATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGC
CAATGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAA
GAGAACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTC
TATCTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGA
ATTTGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGC
CTCTGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAA
GAAATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGA
GGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAG
CGCTATCACCATCCTTCGTATCGATGATAAAATCACTCTTAATCCAAGAGAACAACAAGG
Length of connected seq. 1130
Full length Seq ID -
Full length Seq. -
Length of full length seq. -