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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHJ469
Library CH
(Link to library)
Clone ID CHJ469
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15617-1
Original site URL
Representative seq. ID CHJ469P
(Link to Original site)
Representative DNA sequence 
>CHJ469 (CHJ469Q) /CSM/CH/CHJ4-C/CHJ469Q.Seq.d/
ATTATTTTATTACTAACTTAATATCATATTAAGTTTACATTAAAAATTTAAAAAATAAAT
AAAAAAATAAAAAATTAAATTAAAAAAAAATAAAAAAAATAAAAAAAAATAAAAAAAAAA
ATAAAAANCTAATAATTAAAAAAATTAAATNTTGNAGTAAAAAAATAAATAAATAAATAA
ATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAA
ATAAATAATAATANCATGAGTAACTCAAATAATAATANCTXXXXXXXXXXTGATGATGAT
ACNTNTGCNATCTATAATAAAATTTTGGCTGGTAGAATCACTTTTCCNTTAGGTTTTGAT
GTTGATGCTAAAGATTTAATTAAAAGATTATTAGCTGCTGATAGAACTCGTAGATTGGGT
GCTTTAAAGGATGGTGCATTAGATGTCAAAAATCATAGATGGTTTTCTGATATTAATTGG
GAAAGATTATATCAGAGAAGAGATAATGGACCATTCATTCCNAAGATTCAACATCAAGGT
GATTCTTCNAACTTTGAAATGTATGATGAAGAAGAAATGGTTGAAGAGCCTCCATCTTCN
AATTATGTTGATCCATATGCACATCTTTTCNAGGATTTTTAAAAAATAAAAGATAATAAC
TAATAGAAACTA
sequence update 2002.10.25
Translated Amino Acid sequence 
iilllt*yhikftlki*kinkkikn*ikkk*kk*kkikkkikx**lkklnxxvkk*ink*
ink*ink*ink*ink*ink*INNNXMSNSNNNX---

---DDDTXAIYNKILAGRITFPLGFDVDAKDLIKRLLAADRTRRLGALKDGALDVKNHRW
FSDINWERLYQRRDNGPFIPKIQHQGDSSNFEMYDEEEMVEEPPSSNYVDPYAHLFXDF*
kikdnn**kl
Translated Amino Acid sequence (All Frames) 
Frame A:
iilllt*yhikftlki*kinkkikn*ikkk*kk*kkikkkikx**lkklnxxvkk*ink*
ink*ink*ink*ink*ink*INNNXMSNSNNNX---

---***yxcxl**nfgw*nhfsxrf*c*c*rfn*kiisc**ns*igcfkgwcircqks*m
vf*y*lgkiisekr*wtihsxdstsr*ffxl*nv**rrng*rasifxlc*sictsfxgfl
knkr**liet

Frame B:
lfyy*lniilslh*kfkk*ikk*kiklkknkknkkk*kkk*kxnn*kn*xlx*knk*ink
*ink*ink*ink*ink*ink*iiix*vtqiiix---

---DDDTXAIYNKILAGRITFPLGFDVDAKDLIKRLLAADRTRRLGALKDGALDVKNHRW
FSDINWERLYQRRDNGPFIPKIQHQGDSSNFEMYDEEEMVEEPPSSNYVDPYAHLFXDF*
kikdnn**kl

Frame C:
yfitnlisy*vyiknlknk*knkkln*kkikkikknkkknkxliikkikxxskkink*in
k*ink*ink*ink*ink*ink**xhe*lk**x---

---mmixxxsiikfwlveslfx*vlmlmlki*lkdy*llielvdwvl*rmvh*mskiidg
fliligkdyireeimdhsfxrfnikvilxtlkcmmkkkwlkslhlximlihmhifsrifk
k*kiitnrn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHJ469 (CHJ469Q) /CSM/CH/CHJ4-C/CHJ469Q.Seq.d/                        710   0.0
CHP121 (CHP121Q) /CSM/CH/CHP1-A/CHP121Q.Seq.d/                        680   0.0
CHL103 (CHL103Q) /CSM/CH/CHL1-A/CHL103Q.Seq.d/                        680   0.0
CHI186 (CHI186Q) /CSM/CH/CHI1-D/CHI186Q.Seq.d/                        680   0.0
AHM265 (AHM265Q) /CSM/AH/AHM2-C/AHM265Q.Seq.d/                        680   0.0
SFH701 (SFH701Q) /CSM/SF/SFH7-A/SFH701Q.Seq.d/                        674   0.0
AFI637 (AFI637Q) /CSM/AF/AFI6-B/AFI637Q.Seq.d/                        674   0.0
CHH347 (CHH347Q) /CSM/CH/CHH3-B/CHH347Q.Seq.d/                        658   0.0
CHM664 (CHM664Q) /CSM/CH/CHM6-C/CHM664Q.Seq.d/                        644   0.0
CHK312 (CHK312Q) /CSM/CH/CHK3-A/CHK312Q.Seq.d/                        573   e-162
own update 2002. 9.30
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M38703|M38703.1 Dictyostelium discoideum protein kinase (Dd GPK2) gene, exons 1 and 2.   440   0.0    2
AL662989|AL662989.3 Oryza sativa genomic DNA, chromosome 4, BAC clone: OSJNBa0091C12.    46   6e-04  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38   0.007  12
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    38   0.030  8
AA581319|AA581319.1 nd43b09.r1 NCI_CGAP_AR1 Homo sapiens cDNA clone IMAGE:803033 5', mRNA sequence.    34   0.058  3
AQ635628|AQ635628.1 RPCI-11-480H19.TJ RPCI-11 Homo sapiens genomic clone RPCI-11-480H19, genomic survey sequence.    34   0.073  3
CQ751432|CQ751432.1 Sequence 37366 from Patent WO02068579.         34   0.12   3
AC097276|AC097276.9 Oryza sativa chromosome 3 BAC OJ1519_A12 genomic sequence, complete sequence.    42   0.15   4
BA000021|BA000021.3 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome.    40   0.18   14
AC096288|AC096288.6 Rattus norvegicus clone CH230-38G14, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48   0.19   1
dna update 2005. 1.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

JQ1150(JQ1150)protein kinase (EC 2.7.1.37) cAMP-dependent, catal...   212   9e-54
(P34099) RecName: Full=cAMP-dependent protein kinase catalytic s...   212   9e-54
(P51817) RecName: Full=Serine/threonine-protein kinase PRKX;    ...   107   4e-22
BX322647_6(BX322647|pid:none) Zebrafish DNA sequence from clone ...   105   1e-21
BC091203_1(BC091203|pid:none) Rattus norvegicus protein kinase, ...   101   2e-20
AK149937_1(AK149937|pid:none) Mus musculus bone marrow macrophag...   100   3e-20
Y12638_2(Y12638|pid:none) Trypanosoma brucei brucei brucei AnTat...   100   6e-20
FJ436988_1(FJ436988|pid:none) Phytophthora sojae cAMP-dependent ...   100   6e-20
A84453_1(A84453|pid:none) Sequence 68 from Patent WO9845704.  &A...    99   1e-19
M12303_1(M12303|pid:none) Mouse cAMP-dependent protein kinase ca...    99   1e-19
protein update 2009. 4. 3
PSORT 

 psg: 0.63  gvh: 0.35  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHJ469 is cyt
5' end seq. ID CHJ469F
5' end seq. 
>CHJ469F.Seq
ATTATTTTATTACTAACTTAATATCATATTAAGTTTACATTAAAAATTTAAAAAATAAAT
AAAAAAATAAAAAATTAAATTAAAAAAAAATAAAAAAAATAAAAAAAAATAAAAAAAAAA
ATAAAAANCTAATAATTAAAAAAATTAAATNTTGNAGTAAAAAAATAAATAAATAAATAA
ATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAA
ATAAATAATAATANCATGAGTAACTCAAATAATAATANCTNNNNNNNNNN
Length of 5' end seq. 290
3' end seq. ID CHJ469Z
3' end seq. 
>CHJ469Z.Seq
NNNNNNNNNNTGATGATGATACNTNTGCNATCTATAATAAAATTTTGGCTGGTAGAATCA
CTTTTCCNTTAGGTTTTGATGTTGATGCTAAAGATTTAATTAAAAGATTATTAGCTGCTG
ATAGAACTCGTAGATTGGGTGCTTTAAAGGATGGTGCATTAGATGTCAAAAATCATAGAT
GGTTTTCTGATATTAATTGGGAAAGATTATATCAGAGAAGAGATAATGGACCATTCATTC
CNAAGATTCAACATCAAGGTGATTCTTCNAACTTTGAAATGTATGATGAAGAAGAAATGG
TTGAAGAGCCTCCATCTTCNAATTATGTTGATCCATATGCACATCTTTTCNAGGATTTTT
AAAAAATAAAAGATAATAACTAATAGAAACTA
Length of 3' end seq. 392
Connected seq. ID CHJ469P
Connected seq. 
>CHJ469P.Seq
ATTATTTTATTACTAACTTAATATCATATTAAGTTTACATTAAAAATTTAAAAAATAAAT
AAAAAAATAAAAAATTAAATTAAAAAAAAATAAAAAAAATAAAAAAAAATAAAAAAAAAA
ATAAAAANCTAATAATTAAAAAAATTAAATNTTGNAGTAAAAAAATAAATAAATAAATAA
ATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAA
ATAAATAATAATANCATGAGTAACTCAAATAATAATANCT----------TGATGATGAT
ACNTNTGCNATCTATAATAAAATTTTGGCTGGTAGAATCACTTTTCCNTTAGGTTTTGAT
GTTGATGCTAAAGATTTAATTAAAAGATTATTAGCTGCTGATAGAACTCGTAGATTGGGT
GCTTTAAAGGATGGTGCATTAGATGTCAAAAATCATAGATGGTTTTCTGATATTAATTGG
GAAAGATTATATCAGAGAAGAGATAATGGACCATTCATTCCNAAGATTCAACATCAAGGT
GATTCTTCNAACTTTGAAATGTATGATGAAGAAGAAATGGTTGAAGAGCCTCCATCTTCN
AATTATGTTGATCCATATGCACATCTTTTCNAGGATTTTTAAAAAATAAAAGATAATAAC
TAATAGAAACTA
Length of connected seq. 662
Full length Seq ID -
Full length Seq. -
Length of full length seq. -