>AFG292 (AFG292Q) /CSM/AF/AFG2-D/AFG292Q.Seq.d/
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCA
GGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGXXXXXXXXXXATCAGTGGTA
GATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAA
GCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTT
TAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATC
TTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTG
ATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATC
AAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTG
ATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTA
ATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTC
TTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAG
ATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTC
CATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAG
GTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATATG

THLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILP---

---ISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFK
VENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASV
DIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSH
RSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFRKF
*fys*y

Frame A:
THLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILP---

---ISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFK
VENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASV
DIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSH
RSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFRKF
*fys*y

Frame B:
ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmq
vin*lcknl*fyq---

---svvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlplnsk
lkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qhql
ifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlai
vqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*lsvnf
nfthnm

Frame C:
plitlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssi
rcinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrw
ypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr
**isyariydft---

---qw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*iqs
*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*insis*
ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*ps
fr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcwfnfp*il
illii

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG292 (AFG292Q) /CSM/AF/AFG2-D/AFG292Q.Seq.d/                       2506   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1447   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1447   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1447   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1447   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1447   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1447   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1447   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1441   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1439   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   2e-72  11
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   1e-62  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-60  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-60  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   1e-60  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-58  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   5e-52  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   2e-48  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   5e-46  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   474   0.0
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   343   e-156
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   339   e-153
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   345   e-153
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   328   e-153
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   328   e-153
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   332   e-153
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   343   e-153
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   346   e-152
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   345   e-152

 psg: 0.95  gvh: 0.51  alm: 0.32  top: 0.57  tms: 0.07  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFG292 is cyt

>AFG292F.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCA
GGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAG----------

>AFG292Z.Seq
----------ATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGG
TTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGA
AAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAA
AGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTAT
CTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTC
AATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGT
TGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGG
TATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCAC
TGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCA
TCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGG
TCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGT
TAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATT
TTAATTTTACTCATAATATG

>AFG292P.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCA
GGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAG----------ATCAGTGGTA
GATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAA
GCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTT
TAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATC
TTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTG
ATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATC
AAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTG
ATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTA
ATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTC
TTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAG
ATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTC
CATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAG
GTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATATG