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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHI718
Library CH
(Link to library)
Clone ID CHI718
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U11787-1
Original site URL
Representative seq. ID CHI718P
(Link to Original site)
Representative DNA sequence 
>CHI718 (CHI718Q) /CSM/CH/CHI7-A/CHI718Q.Seq.d/
GTTAAAATTTATATCAATACTCATAATAATAATAATATAGTAACAATAATAATAATAATA
ATATAAAAAAAAAAAATTAATAATAATAGTTTTAAAAATAATATAATATACTANCAATTA
TAATAATAAAAAAAATAAAAAATAAAATAAAATAAAATAAATAAATAGAATTAAAAAAAA
AGGTTAAAAAAAAAAAAAAAAAAAANNCCNAAAAAAAAANCCCNAAAAAAAAAXXXXXXX
XXXTGTAAGATTTCCAGATTTCTTTATGAGTGTTCAGTTGCTAAGTCTTGGTGAATTCCT
TTTCAATATTCAATCAATGGTTTGTGTTTTCAACTATAGTGCATTGGATCCTGAAGAGGT
GAAATTTTGTTCACAATCCCGTTTCTTTGCTTTACCAGTTTTAAATGCCTTACCATATTG
GTGGCGTGTTGCTCAATGTTTTAGACGTTATTATGAAACTCGTCAATTCTTTCCACATAT
TACAAGTGCAATTCGTTCAATCTTTTCAATTATTGCATTGGTTTTAAATTATATTGCATT
GGAATATTCTCAACATGATTGGAGTATTATCAAGATAGCTTGGTTTGGTATTAATGTGGT
TGGTAGCTTTTATAAATTCTATGCCGATATGTCTGTAGATTGGGGTTTCTTTAATAATTA
TAAAACCAATCCTGCT
sequence update 2002.10.25
Translated Amino Acid sequence 
*nlyqys****ysnnnnnnnikkkn****f*k*ynilxiiiikkiknkik*nk*IELKKK
VKKKKKKXXXKKXPKKK---

---VRFPDFFMSVQLLSLGEFLFNIQSMVCVFNYSALDPEEVKFCSQSRFFALPVLNALP
YWWRVAQCFRRYYETRQFFPHITSAIRSIFSIIALVLNYIALEYSQHDWSIIKIAWFGIN
VVGSFYKFYADMSVDWGFFNNYKTNPA
Translated Amino Acid sequence (All Frames) 
Frame A:
vkiyinthnnnnivtiiiiii*kkkinnnsfknniiyxql***kk*kik*nkink*n*kk
rlkkkkkkxpkkkxxkk---

---ckisrflyecsvaksw*ipfqysinglcfql*cigs*rgeilftipflcftsfkclt
ilvaccsmf*tll*nssilstyykcnsfnlfnycigfklycigifst*leyyqdslvwy*
cgw*ll*ilcryvcrlgfl**l*nqsc

Frame B:
lkfisiliiiii**q*****ykkkkliiivlkii*ytxnynnkknkk*nkik*inrikkk
g*kkkkkkxxkkxpxkk---

---VRFPDFFMSVQLLSLGEFLFNIQSMVCVFNYSALDPEEVKFCSQSRFFALPVLNALP
YWWRVAQCFRRYYETRQFFPHITSAIRSIFSIIALVLNYIALEYSQHDWSIIKIAWFGIN
VVGSFYKFYADMSVDWGFFNNYKTNPA

Frame C:
*nlyqys****ysnnnnnnnikkkn****f*k*ynilxiiiikkiknkik*nk*IELKKK
VKKKKKKXXXKKXPKKK---

---*dfqisl*vfsc*vlvnsfsifnqwfvfstivhwilkr*nfvhnpvsllyqf*mpyh
iggvllnvldvimklvnsfhilqvqfvqsfqllhwf*iilhwnilnmigvlsr*lglvlm
wlvafinsmpicl*igvsliiikpil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHI718 (CHI718Q) /CSM/CH/CHI7-A/CHI718Q.Seq.d/                        858   0.0
SSM554 (SSM554Q) /CSM/SS/SSM5-C/SSM554Q.Seq.d/                        793   0.0
VFD732 (VFD732Q) /CSM/VF/VFD7-B/VFD732Q.Seq.d/                         36   0.67
SLB414 (SLB414Q) /CSM/SL/SLB4-A/SLB414Q.Seq.d/                         36   0.67
FC-IC1771 (FC-IC1771Q) /CSM/FC-IC/FC-IC1771Q.Seq.d/                    36   0.67
VHN571 (VHN571Q) /CSM/VH/VHN5-C/VHN571Q.Seq.d/                         34   2.6
VHF608 (VHF608Q) /CSM/VH/VHF6-A/VHF608Q.Seq.d/                         34   2.6
VFM252 (VFM252Q) /CSM/VF/VFM2-C/VFM252Q.Seq.d/                         34   2.6
SSL812 (SSL812Q) /CSM/SS/SSL8-A/SSL812Q.Seq.d/                         34   2.6
SFJ489 (SFJ489Q) /CSM/SF/SFJ4-D/SFJ489Q.Seq.d/                         34   2.6
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z67753|Z67753.1 Odontella sinensis complete chloroplast genome.    46   2e-05  2
CE098810|CE098810.1 tigr-gss-dog-17000371091406 Dog Library Canis familiaris genomic, genomic survey sequence.    48   0.19   1
CC908565|CC908565.1 t043k24ba.f1 TAMBT Bos taurus genomic clone t043k24ba, genomic survey sequence.    48   0.19   1
G83884|G83884.1 S210P6167FD8.T0 BALB/cByJ Mus musculus STS genomic, sequence tagged site.    42   1.1    2
CE466721|CE466721.1 tigr-gss-dog-17000363556115 Dog Library Canis familiaris genomic, genomic survey sequence.    44   3.0    1
AC094350|AC094350.7 Rattus norvegicus clone CH230-3K9, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    34   5.1    7
CR450750|CR450750.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-234P18.    30   5.3    2
AC069057|AC069057.2 Homo sapiens chromosome 2 clone RP11-156G21 map 2, WORKING DRAFT SEQUENCE, 10 unordered pieces.    30   5.4    2
CR352253|CR352253.8 Zebrafish DNA sequence from clone CH211-267D1 in linkage group 13.    30   5.4    2
AC092840|AC092840.4 Homo sapiens BAC clone RP11-258O11 from 2, complete sequence.    30   5.5    2
dna update 2004.12.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54HI2) RecName: Full=SPX and EXS domain-containing protein 4;       303   2e-81
(Q86HQ3) RecName: Full=SPX and EXS domain-containing protein 2; ...   177   2e-43
AF131097_1(AF131097|pid:none) Mus dunni xenotropic and polytropi...    82   2e-14
(Q9QZ71) RecName: Full=Xenotropic and polytropic retrovirus rece...    82   2e-14
(Q9R031) RecName: Full=Xenotropic and polytropic retrovirus rece...    82   2e-14
AF131096_1(AF131096|pid:none) Mus musculus xenotropic and polytr...    80   4e-14
AF131099_1(AF131099|pid:none) Cricetulus griseus xenotropic and ...    75   1e-12
(Q9QZ70) RecName: Full=Xenotropic and polytropic retrovirus rece...    75   1e-12
(Q9TU72) RecName: Full=Xenotropic and polytropic retrovirus rece...    74   4e-12
AL133058_1(AL133058|pid:none) Homo sapiens mRNA; cDNA DKFZp434K0...    72   1e-11
protein update 2009. 4. 2
PSORT 

 psg: 0.39  gvh: 0.59  alm: 0.29  top: 0.40  tms: 0.07  mit: 0.22  mip: 0.05
 nuc: 0.48  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CHI718 is cyt
5' end seq. ID CHI718F
5' end seq. 
>CHI718F.Seq
GTTAAAATTTATATCAATACTCATAATAATAATAATATAGTAACAATAATAATAATAATA
ATATAAAAAAAAAAAATTAATAATAATAGTTTTAAAAATAATATAATATACTANCAATTA
TAATAATAAAAAAAATAAAAAATAAAATAAAATAAAATAAATAAATAGAATTAAAAAAAA
AGGTTAAAAAAAAAAAAAAAAAAAANNCCNAAAAAAAAANCCCNAAAAAAAAANNNNNNN
NNN
Length of 5' end seq. 243
3' end seq. ID CHI718Z
3' end seq. 
>CHI718Z.Seq
NNNNNNNNNNTGTAAGATTTCCAGATTTCTTTATGAGTGTTCAGTTGCTAAGTCTTGGTG
AATTCCTTTTCAATATTCAATCAATGGTTTGTGTTTTCAACTATAGTGCATTGGATCCTG
AAGAGGTGAAATTTTGTTCACAATCCCGTTTCTTTGCTTTACCAGTTTTAAATGCCTTAC
CATATTGGTGGCGTGTTGCTCAATGTTTTAGACGTTATTATGAAACTCGTCAATTCTTTC
CACATATTACAAGTGCAATTCGTTCAATCTTTTCAATTATTGCATTGGTTTTAAATTATA
TTGCATTGGAATATTCTCAACATGATTGGAGTATTATCAAGATAGCTTGGTTTGGTATTA
ATGTGGTTGGTAGCTTTTATAAATTCTATGCCGATATGTCTGTAGATTGGGGTTTCTTTA
ATAATTATAAAACCAATCCTGCT
Length of 3' end seq. 443
Connected seq. ID CHI718P
Connected seq. 
>CHI718P.Seq
GTTAAAATTTATATCAATACTCATAATAATAATAATATAGTAACAATAATAATAATAATA
ATATAAAAAAAAAAAATTAATAATAATAGTTTTAAAAATAATATAATATACTANCAATTA
TAATAATAAAAAAAATAAAAAATAAAATAAAATAAAATAAATAAATAGAATTAAAAAAAA
AGGTTAAAAAAAAAAAAAAAAAAAANNCCNAAAAAAAAANCCCNAAAAAAAAA-------
---TGTAAGATTTCCAGATTTCTTTATGAGTGTTCAGTTGCTAAGTCTTGGTGAATTCCT
TTTCAATATTCAATCAATGGTTTGTGTTTTCAACTATAGTGCATTGGATCCTGAAGAGGT
GAAATTTTGTTCACAATCCCGTTTCTTTGCTTTACCAGTTTTAAATGCCTTACCATATTG
GTGGCGTGTTGCTCAATGTTTTAGACGTTATTATGAAACTCGTCAATTCTTTCCACATAT
TACAAGTGCAATTCGTTCAATCTTTTCAATTATTGCATTGGTTTTAAATTATATTGCATT
GGAATATTCTCAACATGATTGGAGTATTATCAAGATAGCTTGGTTTGGTATTAATGTGGT
TGGTAGCTTTTATAAATTCTATGCCGATATGTCTGTAGATTGGGGTTTCTTTAATAATTA
TAAAACCAATCCTGCT
Length of connected seq. 666
Full length Seq ID -
Full length Seq. -
Length of full length seq. -